维生素A、E对獭兔繁殖性能的影响

将 2 8只繁殖獭兔随机均分 2组 ,基础日粮相同 ,但试验组在配种前 3天妊娠第 7天于日粮中添加VA8mg/kg、VE10 0mg/kg。结果与对照组相比 :试验组产活仔数提高了 2 0 2 3 %(P <0 0 5 ) ,育成率提高了 6 5 8%(P <0 0 5 ) ,增重速度提高 7 11%(P <0 0 5 )。     

北京鸭PRLR基因部分序列的克隆及分析

利用PCR方法克隆了北京鸭催乳素受体(PRLR)基因部分序列,该序列长1325bp。序列分析结果表明,该序列由第9外显子的一部分(57bp)、第9内含子(635bp)以及第10外显子的一部分(633bp)组成;在核苷酸水平上,北京鸭的该序列(除去内含子)与鹅(U07694)、鸡(D13154)、野鸽(DQ209271)、火鸡(L76587)的相似性分别为94.2%、88.7%、86.8%、86.1%;在氨基酸水平上,与鹅(ABA71353)、鸡(BAA02439)、野鸽(AAA20646)、火鸡(AAB01544)的相似性分别为93.5%、84.3%、80.8%、80.9%。     

纳罗克非洲狗尾草的开花习性

纳罗克非洲狗尾草小花开放顺序通常从中上部向基部逐渐开放,最上部小花最后开放;一日开花高峰在清晨3～4时,单个花序开放持续时间26～57d,平均42.5d;逐日开花高峰出现在开花第10～15d;前25d开花数占总数的90%;阴雨天将严重影响小花开放.     

热带亚热带优质高产牧草矮象草的试验研究

10年的系统研究结果表明,矮象草具有适应性与抗逆性强、产量较高质量好、粗蛋白质含量与消化率高、叶量大利用率高、动物利用增产效果显著的特点,是适于热带亚热带地区种植的优良牧草品种.     

蚕丝的纤维化机理研究

本文阐明了以蚕丝蛋白的凝胶－溶胶转移和液晶态,拉伸与液晶的组合纺丝而纺成蚕丝纤维的机理。     

应用RT-PCR方法从进境南美白对虾亲虾中检出桃拉综合征病毒(Taura syndrome virus)

应用PCR和RT—PCR技术，对一批进境的南美白对虾亲虾进行了多种病毒性病原的检疫。结果检出桃拉综合征病毒。临床观察也表明，该批亲虾体表散布大量黑色病灶，为TSV感染引起的TS典型病变。     

欧鳗(Anguilla anguilla)“狂游症”病调查报告

本文在国内首次较系统报告了欧鳗“狂游症”病的流行病学、病理组织学、诊断和防治方法，同时在国内外首次报告了侵袭欧鳗脑部的病毒是该病的致病原，这对今后深入研究起到很大的作用。     

长汀县山麻鸭寄生蠕虫调查及其群落生态分析

剖检长汀县２１羽山麻鸭，检得寄生蠕虫１０种，总感染率为８１％，平均感染强度和密度分别为３６条和３０条。各种蠕虫及其感染分别为：楔形前殖吸虫２８．６％，大多形棘头虫４．％，似椎低颈吸虫４．８％，福建单睾绦虫３３．３￥，美国膜壳绦虫４．８％。     

Effect of dietary supplementation of fish oil for lactating sows and weaned piglets on piglet Th polarization

The present study was designed to investigate the effect of dietary fish oil supplementation on piglet T helper cells (Th) polarization in relation to its impact on piglet serum interferon γ (IFN-γ) and interleukin 10 (IL-10) concentrations and splenic expression of Th1/Th2 characteristic genes. The diets of 18 gestating sows were supplemented with 7% lard (C) (n = 10) or 7% fish oil (T) (n = 8) from 10 d before parturition to weaning. At weaning, a split plot experiment was designed, 56 piglets, 28 each from sows fed with fish oil diet or lard diet, were divided into four groups of 7 replicates (one female and one castrated male per replicate) based on both sow diet during lactation and post-weaning piglet diet (C had 7% lard and T had 7% fish oil): CC, CT, TC, TT, and were fed the 7% fish oil or lard diet from day 35 to day 70. Serum concentrations of IFN-γ and IL-10, and Th1/Th2 related genes expression levels in spleen were measured and analyzed. The results showed that piglets fed with fish oil diet during post-weaning tended to have higher serum IFN-γ/IL-10 ratio (P = 0.09) than lard diet fed piglets. Lactation fish oil feeding increased splenic IL-12b, IL-12 receptor β2 (IL-12Rβ2), IL-2 and IFN-γ genes expression (P < 0.05 or P < 0.01) and post-weaning fish oil feeding increased splenic IL-12b (P = 0.06), IL-2 (P < 0.01) and IFN-γ (P = 0.08) mRNA expression than that in lard diet fed piglets at the end of this experiment. On the other hand, IL-4 gene expression (P = 0.01) in spleen was lower in weaned piglet from fish oil diet fed sows than that from lard diet fed sows. However, post-weaning piglets fed fish oil diet had higher splenic IL-4 (P = 0.06), IL-6 (P < 0.01) and IL-10 (P = 0.05) mRNA abundances than that fed with lard diet. These results indicated that dietary fish oil during lactation could increase Th1 polarization and accelerate immune maturation; while 7% fish oil in weaned piglets' diet was likely to increase Th2 cytokines expression.     

三七总皂甙对大黄鱼肾细胞生长及部分免疫相关基因表达的影响

本试验将三七总皂甙(PNS)体外作用于大黄鱼肾细胞,通过活细胞计数和荧光定量PCR检测,初步了解PNS对大黄鱼肾细胞生长及部分免疫相关因子表达的影响。结果显示,40~200μg/mL浓度的PNS对大黄鱼肾细胞生长无明显影响,但浓度达到1 mg/mL以上时,对细胞生长有负面影响;10~1 000μg/mL浓度的PNS对大黄鱼肾细胞Toll样受体5mRNA的表达有一定的上调作用,但对Toll样受体3的表达有一定的下调作用;PNS浓度为10~100μg/mL时对大黄鱼肾细胞碱性磷酸酶的表达无明显影响,但浓度达到1 000μg/mL时对该因子的表达有下调作用;PNS浓度为10μg/mL时对大黄鱼肾细胞溶菌酶的表达无明显影响,但浓度达到100μg/mL以上时对该因子的表达具有一定的下调作用。