大麦赤霉病抗性的亲子相关分析

在二棱大麦中赤霉病抗性很强的Ｒｕｓｓｉａ６号和六棱大麦中抗性很弱的ＨＥＳ４的杂交后代Ｆ２代的５６０个个体中，以随机抽出８０个个体所展开的Ｆ３—Ｆ５系统来分析赤霉病抗性的亲子相关．结果表明，随着世代的推进，系统内标准差逐渐变小，相关系数逐渐提高，到Ｆ５抗性分离逐渐稳定，Ｆ４个体和由这个个体所展开的Ｆ５系统平均值之间的相关系数为０．６７０，Ｆ４个体所在的系统平均值和Ｆ５系统的平均值之间的相关系数为０．７９０，系统之间的相关系数大于亲代个体和次代系统平均值之间的相关系数．     

A limited role for SsrA/B in persistent Salmonella Typhimurium infections in pigs

Virulence genes regulated by the SsrA/B system are indispensable for systemic disease in BALB/c mice. The role of this regulating system in the pathogenesis of Salmonella Typhimurium infections in pigs is not documented. In the present study, the interactions of Salmonella Typhimurium and an ssrA/B mutant were compared in vitro and in vivo. The ssrA/B mutant strain displayed decreased Salmonella Pathogenicity Island 2 (SPI-2) expression levels, showed a replication defect in mouse macrophages and was attenuated in a mouse model after oral inoculation. Using real time qRT-PCR and a porcine ileal loop model, it was shown that the ssrA/B mutant strain was not significantly attenuated in overall virulence and SPI-1 expression in specific. Flowcytometric analysis demonstrated that the ssrA/B mutant strain was defective in intracellular replication in porcine macrophages. After oral inoculation of piglets with the wild type strain or the ssrA/B mutant strain, the animals of both groups excreted Salmonella and were colonized by Salmonella to the same extent. In an intravenous mixed infection model, the ssrA/B mutant strain was defective in the colonization of several internal organs. These results suggest that the ssrA/B gene of Salmonella Typhimurium plays a limited role in the persistent intestinal colonization of pigs.     

Effect of chorionic gonadotropin administration on the concentration of testosterone in the blood of boars]

In intact boars, intravenous administration of 100 to 6000 I. U. chorionic gonadotropin elicited a pronounced rise in the plasma level of testosterone, depending on the time interval from the administration of chorionic gonadotropin and on the size of the administered dose. In the blood of castrated boars such an application of choronic gonadotropin did not influence the concentration of testosterone. It was inferred that the rise in the levels of blood testosterone in boars after administration of chorionic gonadotropin was specific for testicular incretion and indicated the endocrine reserve of the testes. There is a discussion concerning the importance of the functional testing of the incretion capacity of testes for differential diagnosis different forms o incretion hypogonadism.     

Effect of bilateral orchidectomy on serum testosterone levels in boars]

Bilateral orchidectomy caused a marked decrease in the concentration of testosterone in the bood plasma of boars already in the early post-castration stage of three hours after the operation. In the period from 3 to 207 days after castration, the pre-operation levels of plasma testosterone decrease by 84.4%, on an average. Adreno-cortical compensation of the concentration of the hormone did not occur in the post-castration period. The conclusion is that the levels of testosterone in the blood of boars are mostly of testicular origin and can be considered as the main criterion of the incretion function of the testes. The discussion concerns the practical importance of the determination of testosterone levels in the blood of boars for clinical evaluation of the hormonal function of the testes and for diagnostics of incretion hypogonodism.     

Genomic signatures predict migration and spawning failure in wild Canadian salmon

Long-term population viability of Fraser River sockeye salmon (Oncorhynchus nerka) is threatened by unusually high levels of mortality as they swim to their spawning areas before they spawn. Functional genomic studies on biopsied gill tissue from tagged wild adults that were tracked through ocean and river environments revealed physiological profiles predictive of successful migration and spawning. We identified a common genomic profile that was correlated with survival in each study. In ocean-tagged fish, a mortality-related genomic signature was associated with a 13.5-fold greater chance of dying en route. In river-tagged fish, the same genomic signature was associated with a 50% increase in mortality before reaching the spawning grounds in one of three stocks tested. At the spawning grounds, the same signature was associated with 3.7-fold greater odds of dying without spawning. Functional analysis raises the possibility that the mortality-related signature reflects a viral infection.     

麦类赤霉病菌菌丝的伸长速度及其温度反应

供试赤霉病菌２４９个菌株，探讨菌丝的伸长速度和对温度的反应，结果表明：２５℃条件下，菌丝的伸长速度在菌株之间的差异很大，对高温或低温的耐性程度在菌株之间也有很大的差异，缩小菌株数，扩大温度梯度所做了试验结果表明：菌株之间对高温和低温耐性、温度反应、最适菌丝伸长温度等都有很大的差异。     

Sorption properties of iron impregnated activated carbon web for removal of methylene blue from aqueous media

In this study, impregnation of iron chloride was carried out on needle punched web of waste acrylic fibers, which was subsequently carbonized under layer of charcoal by physical activation in high temperature furnace to produce iron impregnated activated carbon (FeAC). For comparison purpose, one more sample of activated carbon (AC) was prepared without impregnation of iron chloride. Both the webs were carbonized at 1200 °C with no holding time, and characterization of BET surface area, SEM morphology, EDX elemental analysis, XRD crystalline structure was performed. The FeAC web was used as adsorbent for the removal of methylene blue from aqueous solution. The dye removal percentage was investigated at different experimental parameters like different dye concentrations, adsorbent dosage, stirring speed and different pH. The obtained results were analyzed using linear and non-linear forms of Langmuir and Freundlich isotherms and adsorption kinetics (i.e. pseudo first order and pseudo second order model).     

Identification of monoclonal antibodies against 2,4-D herbicide by ELISA and DNA sequencing

Seven hybridoma clones, E2/G2, E2/B5, E4/C2, G5/E10, F6/C10, B5/C3, and B7, produced within one fusion experiment in 1991 and the clone E4/C2 originated from 1995 were characterized by sequencing of genes coding for variable domains of the antibodies against 2,4-D herbicide. Amino acid sequences of selected antibodies, deduced from DNA analysis, were confirmed by mass spectrometry. Surprisingly, nucleotide sequence analysis of the clones E2/G2 and E2/B5, producing the most sensitive antibodies, proved to have sequence homology of their variable domains, although the IC(50) values determined for these antibodies 9 years prior to the DNA analysis were 2.0 and 8.2 ng/mL, respectively. The same findings arose from the comparison of the immunochemical to DNA data established for G5/E10, F6/C10, and B5/C3 clones producing antibodies with IC(50) values in the range of 26.3-43.1 ng /mL. The clone E4/C2, originating from the later fusion experiment, did not share nucleotide homology with any of the examined clones. Data obtained by ELISA, immunosensor, and DNA analysis within a 9 year period are discussed with respect to hybridoma stability, methodic artifacts, measurement reliability, and other possible factors influencing the result interpretation.     

Breeding for value in a changing world: past achievements and future prospects

Large-scale tree improvement programs began in the 1950s. Tree improvement is now part of operational silviculture programs in many companies and countries around the world and tree breeding programs have produced very impressive results: (1) realized gains in plantations being established today of some 40–50 % in volume yield above unimproved material for many programs; (2) increased efficiencies in all aspects of breeding, selection, testing and deployment; and (3) a shortening of the generation interval by a factor of two from approximately 30 years in the first generation to less than 15 years today for pine programs. What about the future? What should tree breeders be thinking, planning and doing to ensure that results 60 years from now are even more impressive than those from the previous 60 years? Tree breeders today live in a rapidly changing world faced with: increasing demands for food, energy and water; globalization leading to an interconnectedness of markets and rapid spread of exotic organisms; climate change and its implications for genetic deployment; burgeoning technology in robotics, communications and molecular tools; shifting ownership patterns of forest land; and the real possibility of completely new forest products and markets in the future. Three ideas for “Breeding for Value in a Changing World” are: (1) adopt a robust philosophy that aims to ensure maximum value produced per ha even in a future world that will be quite different; (2) embrace technology at every phase in the tree improvement process; and (3) encourage interdisciplinary teams of scientists to solve complex problems that require expertise ranging from molecular to landscape scales.