Sensitivity and specificity of latex agglutination tests used to identify Streptococcus agalactiae and Staphylococcus aureus isolated from bulk tank milk

Comparisons were made among rapid latex agglutination tests and conventional biochemical tests used to identify Streptococcus agalactiae and Staphylococcus aureus. Ninety-eight streptococci and 149 staphylococci isolated from bulk tank milk were tested. Sensitivity and specificity for the latex agglutination test used for identification of Str agalactiae were 97.6 and 98.2%, respectively. Sensitivity and specificity for the latex agglutination test used for identification of S aureus were 90.2 and 67.5%, respectively. Of 25 staphylococci considered false-positive by the latex agglutination test, 14 (56%) were considered tube coagulase-positive. Fifteen staphylococci considered false-positive by latex agglutination test had biotypes representative of S hyicus of S xylosus.     

Cranial vena caval syndrome secondary to transvenous pacemaker implantation in two dogs

Superior vena caval syndrome is a rare, but reported complication of transvenous pacemaker implantation in humans. This syndrome can occur secondary to fibrotic and/or thrombotic obstruction of venous blood flow into the right atrium. The therapeutic approach depends on the suspicion of the presence of an active thrombus and may include antithrombotics, angioplasty and/or surgical venoplasty. We describe two dogs that developed severe pleural effusion secondary to stricture formation in the cranial vena cava 4 years after dual chamber transvenous pacemaker implantation. The stenosis was most likely due to fibrosis secondary to the transvenous pacemaker leads. Balloon angioplasty of the lesion resulted in resolution of the pleural effusion in both patients. Balloon angioplasty appears to be a viable therapeutic approach in dogs with cranial vena caval syndrome caused by focal stenotic lesions.     

Simulated rainfall evaluation of revegetation/mulch erosion control in the Lake Tahoe basin—3: soil treatment effects

Revegetation, or other erosion control treatments of disturbed soil slopes in forested areas and along highways of the Lake Tahoe basin are directed at reduction of sediment loading to waterways reaching the lake. However, following treatment, little vegetation monitoring, or hydrologic evaluation has been conducted either to determine if the various treatments are successful or to assess the duration of erosion control anticipated in the field. Here, we build upon results from use of the portable rainfall simulator (RS) described in the first two papers of this series to evaluate cover and revegetation treatment effects on runoff rates and sediment concentrations and yields from disturbed granitic and volcanic soils in the basin. The effects of slope on rainfall runoff, infiltration and erosion rates were determined at several revegetated road cut and ski run sites. Rainfall simulation (∼60 mm h⁻¹, approximating a 100‐year, 15‐minute storm) had a mean drop size of ∼2·1 mm and approximately 70 per cent of ‘natural’ rainfall kinetic energy. Measurements of: time to runoff; infiltration; runoff amount; sediment yield; and average sediment concentration were obtained. Runoff sediment concentrations and yields from sparsely covered volcanic and bare granitic soils can be correlated to slope. Sediment concentrations and yields from nearly bare volcanic soils exceeded those from granitic soils by an order of magnitude across slopes ranging from 30–70 per cent. Revegetation, or application of pine‐needle mulch covers to both soil types dramatically decreased sediment concentrations and yields. Incorporation of woodchips or soil rehabilitation that includes tillage, use of amendments (biosol, compost) and mulch covers together with plant seeding resulted in little or no runoff or sediment yield from both soils. Repeated measurements of sediment concentrations and yields in the subsequent two years following woodchip or soil rehabilitation treatments continued to result in little or no runoff. Revegetation treatments involving only use of grasses to cover the soils were largely ineffective due to sparse sustainable coverage (< 35 per cent) and inadequate infiltration rates. Copyright © 2005 John Wiley & Sons, Ltd.     

Physiological and morphological responses to nitrogen limitation in jack pine seedlings: potential implications for drought tolerance

The morphological and physiological responses to nitrogen (N) limitation in jack pine (Pinus banksiana Lamb.) seedlings were studied following the initiation of four different dynamic N treatments for six and 15 weeks. The N treatments produced needle N concentrations from 11 to 31 mg g^-1dry weight, and seven-fold difference in dry weight at 15 weeks. Low-N jack pine seedlings: 1) had an higher root/shoot ratio; 2) extended their tap root more rapidly; 3) were better able to maintain turgor when shoot water potential declined; and 4) had a larger dry weight fraction and apoplasmic fraction than seedlings with higher foliar N concentrations. These responses may contribute collectively to enhance drought tolerance in N-limited plants, thereby affecting seedling quality. Modifying nursery fertilization regimes, other than optimal as usually applied, may thus be needed to produce stock for use on particularly droughty sites. Knowledge of the nature of drought at a particular site could be an important consideration when making decisions related to fertilization.     

Incomplete movement of Cryphonectria hypovirus 1 within a vegetative compatibility type of Cryphonectria parasitica in natural cankers on grafted American chestnut trees

American chestnut trees, grafted in 1980 from large survivors, were inoculated in 1982 and 1983 with four white (European) hypovirulent strains of Cryphonectria parasitica, infected with C. hypovirus 1 (CHV1); this hypovirus has been shown to be capable of moving rapidly within the mycelium of a vegetative compatibility (vc) type of C. parasitica in blight cankers. Using a 49‐cell lattice plot, 17.8×17.8 cm, the spatial patterns and frequencies of white and pigmented isolates and white and pigmented vc types were investigated within superficial cankers on the grafts located outside the hypovirulent‐strain‐inoculated zone. Four of six cankers assayed contained white isolates, and three of the four had random spatial patterns of white isolates, based on join‐count statistics. Vc tests, using pigmented isolates and pigmented single‐spore colonies of white isolates, indicated that the majority of white and pigmented isolates recovered from each of two cankers assayed were in one vc type. White and pigmented lattice‐plot cells of the same vc type were frequently in contact with each other, indicating incomplete movement of CHV1 within a vc type. Nine and 10 vc types were found in the two cankers; it is hypothesized that small, white vc type areas in each canker may be a source of CHV1 transmission to the major vc types. Based on join‐count statistics, the spatial pattern of the single, major vc type in one canker was non‐random (aggregated), whereas the other canker had a random major vc type pattern. White and pigmented in vitro variants (sectors) of C. parasitica, that resemble white and pigmented in vivo variants in spatial contact and vc compatibility, were intermediate hypovirulent and virulent on forest American chestnuts, and dsRNA positive and negative, respectively. Incomplete movement of CHV1 within a vc type could be a major cause of the prevalence of pigmented isolates in superficial cankers on chestnut trees.