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41.
42.
Kawakatsu H 《Science (New York, N.Y.)》2012,335(6075):1448-1449
43.
Kojima D Hatai H Oyamada T Park CH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(8):1045-1049
Extraskeletal myxoid chondrosarcoma was found in a five-month-old male Irish setter dog. At necropsy, the largest mass, measuring 15 × 13 × 13 cm, was found in the right caudal lobe of the lung, and metastatic small masses were observed in multiple organs. Microscopically, the tumor comprised mainly atypical mesenchymal cells and myxoid stroma, which stained positively with Alcian blue. Immunohistochemically, the tumor cells stained positively for vimentin, S-100 protein, neuron-specific enolase, calretinin, and chromogranin A. Ultrastructurally, the cytoplasm of the tumor cells was comprised abundant rough endoplasmic reticulum, mitochondria, Golgi complex, free ribosomes and short irregular microvillous processes extending from the cytoplasm. Based on these pathological findings, this tumor was diagnosed as extraskeletal myxoid chondrosarcoma. 相似文献
44.
Park CH Kojima D Hatai H Inoue S Oyamada T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(6):787-790
Traumatic neuroma of the cervical spinal cord was diagnosed in a 14-year-old male mixed-breed dog. A gross view showed two intradural extramedullary masses, measuring 1 and 0.6 cm in length and 0.7 and 0.4 cm in diameter, attached to the left side of the spinal cord at the level of the sixth and seventh cervical vertebrae. Microscopically, the cervical spinal masses comprised interlacing fascicles of axons and Schwann cells surrounded by collagenous stroma. Immunohistochemically, the fascicles were stained positively for neurofilament and S-100 proteins. Ultrastructurally, variably sized myelinated fibers and onion bulb-like structures were observed. To our knowledge, this is the first report of a traumatic neuroma in the cervical spinal cord of a dog. 相似文献
45.
Tatsuyuki YOSHIDA Makoto SEKI Norio WATANABE Hiroki FURUTA Itaru YOSHIMURA Masahiro OSADA Koji CHIBA Konosuke OKADA Koh KAWASUMI Hitoshi USHIJIMA 《Animal Science Journal》2012,83(3):207-212
Diagnosis of corpus luteum (CL) function by rectal palpation (RP) has been widely used for recipient selection of embryo transfer (ET), a technology essential for genetic improvements in cattle. To examine the accuracy of RP diagnosis method, the relationship between RP‐based CL function and reproductive performance was compared in this study. In Experiment 1, CL of Holstein heifers on day 7 after estrus was classified into functional or hypoplastic by RP, and the results were compared with ultrasonographic (US) images and plasma progesterone (P4) levels. As a result, heifers with functional CL judged by RP had a mean maximum CL diameter of 20.1 ± 3.1 mm on US and a mean P4 concentration of 8.1 ± 2.3 ng/mL. These values were significantly greater than those of heifers with hypoplastic CL (12.4 ± 5.4 mm, 4.0 ± 2.8 ng/mL) (P < 0.001). In Experiment 2, the length of the estrus cycle was examined between functional CL and hypoplastic CL. The rate of heifers with a normal estrus cycle length with 18–25 days was significantly lower with hypoplastic CL than with functional CL (16/24 vs. 43/46, P < 0.01). In Experiment 3, 543 inseminated heifers were similarly classified by CL function by RP 7 days after estrus. The heifers with functional CL showed higher pregnancy rate compared with the heifers with hypoplastic CL (75.2 vs. 47.9%, P < 0.0001). Finally, the CL function of 66 heifers was examined by RP on day 7 post‐estrus, and ET was performed in 49 (74.2%) heifers with functional CL. As a result, 27 (55.1%) of them became pregnant. Taken together, these results reconfirm that RP on day 7 after estrus is useful for selection of heifers with functional CL. 相似文献
46.
Masako Seki Makiko Chono Tsutomu Nishimura Mikako Sato Yasuhiro Yoshimura Hitoshi Matsunaka Masaya Fujita Shunsuke Oda Katashi Kubo Chikako Kiribuchi-Otobe Hisayo Kojima Hidetaka Nishida Kenji Kato 《Breeding Science》2013,63(3):309-316
The Ppd-A1 genotype of 240 Japanese wheat cultivars and 40 foreign cultivars was determined using a PCR-based method. Among Japanese cultivars, only 12 cultivars, all of which were Hokkaido winter wheat, carried the Ppd-A1a allele, while this allele was not found in Hokkaido spring wheat cultivars or Tohoku-Kyushu cultivars. Cultivars with a photoperiod-insensitive allele headed 6.9–9.8 days earlier in Kanto and 2.5 days earlier in Hokkaido than photoperiod-sensitive cultivars. The lower effect of photoperiod-insensitive alleles observed in Hokkaido could be due to the longer day-length at the spike formation stage compared with that in Kanto. Pedigree analysis showed that ‘Purple Straw’ and ‘Tohoku 118’ were donors of Ppd-A1a and Ppd-D1a in Hokkaido wheat cultivars, respectively. Wheat cultivars recently developed in Hokkaido carry photoperiod-insensitive alleles at a high frequency. For efficient utilization of Ppd-1 alleles in the Hokkaido wheat-breeding program, the effect of Ppd-1 on growth pattern and grain yield should be investigated. Ppd-A1a may be useful as a unique gene source for fine tuning the heading time in the Tohoku-Kyushu region since the effect of Ppd-A1a on photoperiod insensitivity appears to differ from the effect of Ppd-B1a and Ppd-D1a. 相似文献
47.
Ardiansyah Ohsaki Y Shirakawa H Koseki T Komai M 《Journal of agricultural and food chemistry》2008,56(8):2825-2830
We studied the effects of a single oral administration of ferulic acid (FA) on the blood pressure (BP) and lipid profile in stroke-prone spontaneously hypertensive rats (SHRSP). Male 12-week-old SHRSP were administered FA (9.5 mg/kg of body weight) and distilled water as the control (C) (1 mL) via a gastric tube. The hypotensive effect of FA was observed at the lowest value after 2 h administration. A decrease in the angiotensin-1-converting enzyme (ACE) activity in the plasma corresponded well with the reduction of BP. Plasma total cholesterol and triglyceride levels were lower after 2 h administration. The mRNA expression of genes involved in lipid and drug metabolism was downregulated in the FA group. These results suggest that oral administration of FA appears beneficial in improving hypertension and hyperlipidemia. 相似文献
48.
Masahide Aikawa Takatoshi Hiraki Hitoshi Mukai Kentaro Murano 《Water, air, and soil pollution》2008,190(1-4):287-297
A field survey on the concentration of chemical species in particulate matter and gaseous compounds at two monitoring sites with different site classifications (urban and rural) was conducted over three years. Total (particulate matter + gaseous compounds) concentrations at the rural site were significantly lower than those at the urban site for all species (sulfur $\left( {{\text{SO}}^{{{\text{2 - }}}}_{{\text{4}}} {\left( {\text{p}} \right)}} \right.$ and SO2(g)), nitrate ${\text{(NO}}_{{{\text{3}}^{{\text{ - }}} }} {\left( {\text{p}} \right)}$ and HNO3(g)), ammonium ${\text{(NH}}_{{{\text{4}}^{{\text{ + }}} }} {\text{(p)}})$ and ammonia (NH3(g)), and chloride (Cl? (p) and HCl (g))), which is thought to reflect classification of the site. The difference in the sulfur concentration at the urban and rural sites was characterized by the difference in SO2 (g) concentration. Further, a clear seasonality was observed for the nitrate species. The HNO3 (g) concentration was high in the summer compared with other seasons at both the urban and rural sites. The ${\text{NH}}_4^ + \left( {\text{p}} \right)$ concentration levels were approximately the same as those of NH3 (g) at both sites. The molar ratios of the particulate matter concentration to the total concentration showed different characteristics; the nitrate, ammonium and ammonia, and chloride species showed a clear seasonal variation: low in summer and high in winter and the values were similar regardless of the site. On the other hand, the sulfur species showed constant values at both the urban and rural sites, however the concentrations were significantly different for the two sites. Ammonium accounted for the largest proportion of cations in the particulate matter, regardless of the site classification. In contrast, ${\text{SO}}_4^{2 - } \left( {\text{p}} \right)$ accounted for the largest proportion of anions at the rural site, whereas ${\text{NO}}_3^ - \left( {\text{p}} \right)$ was comparable to ${\text{SO}}_4^{2 - } \left( {\text{p}} \right)$ at the urban site. Ammonia accounted for the largest proportion of all chemical species at both sites. Seasonal analysis of the proportional distribution in particulate matter and gaseous compounds provides information on atmospheric conditions. 相似文献
49.
The total cell numbers (TCNs) of bovine embryos collected from superovulated donors (VIVO embryos) were counted 0-9 d after ovulation to quantify the developmental process. Using numerical analysis of embryo development, we also compared the developmental process of VIVO embryos, in vitro-fertilized (IVF) embryos and nuclear transfer (NT) embryos obtained from enucleated oocytes and blastomere nuclei. The TCNs of embryos were measured using the air-dry method. Cleavage divisions (CD) of the embryos were obtained using logarithmic transformation of the TCN. The TCN of the VIVO embryos increased significantly (P<0.001) with time. The relationship between the CD of the VIVO embryos at 0-9 d after ovulation and age in days was described by a linear equation with a high correlation (y=1.03x+0.16, r=0.99), showing that CD occurs about once each day for all blastomeres. However, compared to the VIVO embryos, the TCN of the IVF embryos did not increase from 3-4 d nor after 7 d; the TCN of the NT embryos did not increase after 7 d (P>0.05). The results suggest a delay in development at these developmental stages. The slopes of regression lines of the IVF and NT embryos were significantly (P<0.001) smaller, indicating that quantification of the developmental process of VIVO embryos according to TCN and CD would be useful as criteria for numerical evaluation of the developmental process of bovine in vitro produced embryos. 相似文献
50.
Yuka Maeda Keiko Nishimura Kazuhiro Kurosu Hitoshi Mizuguchi Shigeru Sato Fuminori Terada Shiro Kushibiki 《Animal Science Journal》2019,90(4):523-532
This study aimed to examine the effects of feeding kraft pulp (KP) on the growth performance, feed digestibility, and rumen fermentation of Japanese Black fattening steers. Ten Japanese Black fattening steers (aged 26 months) were randomly divided into control and KP groups. The control group (n = 5) was fed concentrate feed without KP, and the KP group (n = 5) was fed concentrate feed containing 10% KP. Both the groups were provided rice straw as roughage. The experiment was conducted over a period of 12 weeks. There was no significant difference in dry matter intake, daily body weight gain, and nutrient digestibility between both groups. No difference was observed in the ruminal concentrations of volatile fatty acids among the groups. At weeks 8 and 12 after the onset of the experiment, the acetate‐to‐propionate ratio in the ruminal fluid of the KP group was significantly higher than that of the control group. The average daily pH of ruminal fluid and activity of ruminal lipopolysaccharide did not differ between the groups. Our results suggested that the growth performance and feed digestibility in the Japanese Black fattening steers were not influenced by replacing concentrate feed with KP. 相似文献