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61.
Hartmann H Baumgardt T Höchel J 《Berliner und Münchener tier?rztliche Wochenschrift》2006,119(1-2):62-67
Established renal function tests for the quantitative determination of the glomerular filtration rate (GFR) in small animals by means of an exogenous clearance marker like creatinine are based on the intravenous or subcutaneous administration of the marker. In order to simplify performing the test, the suitability of the peroral administration of the marker substance was tested. Exogenous creatinine was administered to 17 Beagle dogs successively by the peroral (dose: 4 g/m2 BSA) and the subcutaneous route (dose: 2 g/m2 BSA). Both routes were tested sequentially in fasted and fed animals. In addition to the peroral administration of creatinine, the absorption marker D-Xylose (dose: 0.5 g/kg body weight) was given per os. Pharmacokinetic parameters were calculated based on serum concentration--time data of both markers. Maximum serum concentrations of the exogenous creatinine (C(max) = 1284 +/- 173 micromol/l) were observed 92 +/- 19 min post-dose (t(max)) in fasted dogs after peroral administration of creatinine. C(max) (956 +/- 209 micromol/l) and t(max) (67 +/- 13 min) were statistically significantly reduced in fed animals. The exogenous plasma clearance of creatinine was about 1/3 lower in fasted animals (94 +/- 15 ml/min/m2) than in fed ones (134 +/- 28 ml/min/m2). The apparent terminal disposition half-life of the exogenous creatinine showed mean values of about 170 min (fasted) and 200 min (fed). After peroral administration of D-Xylose, fasted animals showed higher C(max) (3.9 +/- 0.99 mmol/l) and t(max) values (60 +/- 18 min) than fed dogs (C(max) = 2.2 +/- 0.55 mmol/l, t(max) = 40 +/- 15 min). C(max) and t(max) did not differ between fed and fasted dogs after subcutaneous administration of creatinine. Creatinine clearance was again higher in fed (124 +/- 12.8 ml/min/m2) than in fasted dogs (104 +/- 9.0 ml/min/m2) after subcutaneous administration of the marker. The terminal disposition half-live was, however, similar with about 130-140 min. The route of administration (peroral vs. subcutaneous) did not influence the calculated clearance (no statistical significance when p < 0.01 is required). Creatinine in a dose of 4 g/m2 BSA can be administered by the peroral route of administration for assessing the GFR. For the quantitative determination of GFR standardized condition are required, i.e. animals have to be fasted for > or = 6 hours. 相似文献
62.
Franco Barbieri Claudio Colosio Helmut Schlitt Marco Maroni 《Pest management science》1995,43(4):259-262
The pharmacokinetics of pentachlorophenol (PCP) excretion in urine was examined in four workers exposed to the biocide in the tannery and wood industries. PCP concentration in urine was significantly higher in morning samples than in afternoon or evening samples. This excretion pattern was observed during an exposure of several shifts and following a four-week holiday period. The estimated half-life for PCP urine excretion was 10 days. Biological monitoring of occupational exposure to PCP should be carried out by measuring the concentration of the compound in urine collected in the morning or before the workshift. 相似文献
63.
Rabsch W Mirold S Hardt WD Tschäpe H 《Berliner und Münchener tier?rztliche Wochenschrift》2002,115(9-10):355-359
Salmonella bacteriophages seem to mediate horizontal transfer of virulence functions among Salmonella strains in two different ways: by general transduction and also by lysogenic conversion. The majority of wild phages isolated from Salmonella strains belong to the P22 like phages and were able to transduce. Our data show that the lysogenic conversion is generally accompanied by changes in the susceptibility to the typing phages used for epidemiological purposes. Similar phage type conversions to S. Typhimurium DT104 could be detected upon lysogenization with two other S. Typhimurium strains. For some S. Typhimurium strains the typical phage pattern is actually associated with alterations of virulence characteristics. For example, all tested wild type isolates of phage types DT49 and DT204 were found to be SopE phi-lysogens. The Anderson typing phages interfere with the prophages and/or cryptic phages and so the complex genetic short-term evolution can be demonstrated in the lab. This is one reason for the successful application of phage typing in Salmonella epidemiology since the 50s. 相似文献
64.
65.
Kirsch P Nusser P Hotzel H Moser I 《Berliner und Münchener tier?rztliche Wochenschrift》2008,121(7-8):270-277
During regular health status monitoring of the colony of amphibian, Mycobacterium (M.) gordonae were isolated from granulomatous lesions of the tiptoes from the South African clawed frog (Xenopus laevis) maintained at the Tierforschungszentrum of the University of Ulm. During a period of three years a total of 21 animals of the colony, consisting of 350-400 frogs, showed granuloma of the tip of the toes and a loss of the claws. The general condition and the behavior of the frogs appeared to be unchanged. Using a selective medium one isolate was cultured and identified by sequencing of the 16S rRNA gene. To apply a rapid diagnostic method for detecting mycobacteria, in particular M. gordonae in the health monitoring programme of the Xenopus laevis colony, we established the rpoB gene PCR followed by HaeIII restriction analysis of the PCR product. We identified M. gordonae from granuloma of the tiptoes and from unaltered tissue samples of the lungs and skin by PCR restriction analysis. Since mycobacterial species apparently are widespread in granulomatous lesions of the tiptoes of Xenopus laevis, we hypothesize a pathogenic potential. This view is supported by an increasing number of reports in the literature on infections with nontuberculous, "non-pathogenic" mycobacteria in Xenopus laevis. 相似文献
66.
Szeredi L Tenk M Jánosi S Pálfi V Hotzel H Sachse K Pospischil A Bozsó M Glávits R Molnár T 《Acta veterinaria Hungarica》2008,56(3):353-367
Cases of equine abortion and perinatal foal losses were investigated in Hungary during a three-year period (1998-2000). Samples from aborted equine fetuses and newborn foals (total n = 96) were examined using bacteriological, virological, pathological, immunohistochemical (IHC), molecular biological and serological methods. The cause of abortion and perinatal foal loss was identified in 67/96 cases (70%); viral infection was found in 22 (23%), viral and bacterial coinfection in 1 (1%), bacterial infection in 23 (24%), protozoan infection in 1 (1%) and fungal infection in 2 cases (2%). Morphological lesions suggestive of infection were recorded in 2 (2%) and non-infectious causes in 16 cases (17%). 相似文献
67.
Small-scale effects of historical land use and topography on post-cultural tree species composition in an Alpine valley in southern Switzerland 总被引:1,自引:0,他引:1
Investigations of spatial patterns in forest tree species composition are essential in the understanding of landscape dynamics,
especially in areas of land-use change. The specific environmental factors controlling the present patterns, however, vary
with the scale of observation. In this study we estimated abundance of adult trees and tree regeneration in a Southern Alpine
valley in Ticino, Switzerland. We hypothesized that, at the present scale, spatial pattern of post-cultural tree species does
not primarily depend on topographic features but responds instead to small-scale variation in historical land use. We used
multivariate regression trees to relate species abundances to environmental variables. Species matrices were comprised of
single tree species abundance as well as species groups. Groups were formed according to common ecological species requirements
with respect to shade tolerance, soil moisture and soil nutrients. Though species variance could only be partially explained,
a clear ranking in the relative importance of environmental variables emerged. Tree basal area of formerly cultivated Castanea sativa (Mill.) was the most important factor accounting for up to 50% of species’ variation. Influence of topographic attributes
was minor, restricted to profile curvature, and partly contradictory in response. Our results suggest the importance of biotic
factors and soil properties for small-scale variation in tree species composition and need for further investigations in the
study area on the ecological requirements of tree species in the early growing stage. 相似文献
68.
Ralf ELVERT Nicole EHRHARDT Martin TAUBE Martin KLINGENSPOR Valerie GAILUS‐DURNER Helmut FUCHS Martin Hrabe DE ANGELIS Veronique BLANQUET Gerhard HELDMAER 《Integrative zoology》2006,1(3):122-125
The German Mouse Clinic was established as a phenotyping center to provide the scientific community with systematic standardized phenotyping of mouse models from various genetic backgrounds. We found metabolic phenotypes in nine out of 20 mutant lines screened in a primary screen. Based on these findings, the mutants were analyzed in secondary and tertiary screens. Mice of a sample mutant line, isolated from the ENU‐screen at the National Research Center for Environment and Health in Munich, were found to have lower body weight, consume less food, but have higher ratios of metabolized energy per unit body weight compared with their wild‐type littermates. Basal metabolic rate and heat production were simultaneously increased by 16–18%, whereas body fat content was reduced by 11–16%. The combination of various parameters of energy consumption, expenditure and energy storage illustrate the metabolic demands of the sample mutant mouse line and demonstrate the utility of the powerful phenotyping tool used at the German Mouse Clinic. 相似文献
69.
Productivity is a poor predictor of plant species richness 总被引:5,自引:0,他引:5
Adler PB Seabloom EW Borer ET Hillebrand H Hautier Y Hector A Harpole WS O'Halloran LR Grace JB Anderson TM Bakker JD Biederman LA Brown CS Buckley YM Calabrese LB Chu CJ Cleland EE Collins SL Cottingham KL Crawley MJ Damschen EI Davies KF DeCrappeo NM Fay PA Firn J Frater P Gasarch EI Gruner DS Hagenah N Hille Ris Lambers J Humphries H Jin VL Kay AD Kirkman KP Klein JA Knops JM La Pierre KJ Lambrinos JG Li W MacDougall AS McCulley RL Melbourne BA Mitchell CE Moore JL Morgan JW Mortensen B 《Science (New York, N.Y.)》2011,333(6050):1750-1753
For more than 30 years, the relationship between net primary productivity and species richness has generated intense debate in ecology about the processes regulating local diversity. The original view, which is still widely accepted, holds that the relationship is hump-shaped, with richness first rising and then declining with increasing productivity. Although recent meta-analyses questioned the generality of hump-shaped patterns, these syntheses have been criticized for failing to account for methodological differences among studies. We addressed such concerns by conducting standardized sampling in 48 herbaceous-dominated plant communities on five continents. We found no clear relationship between productivity and fine-scale (meters(-2)) richness within sites, within regions, or across the globe. Ecologists should focus on fresh, mechanistic approaches to understanding the multivariate links between productivity and richness. 相似文献
70.
The downward velocity of Pb and Cs in undisturbed European forest soils is determined from the depth distribution of atmospheric 210Pb , and from the penetration depth of the bomb Cs peak in the soil. The downward velocity of Pb and Cs shows no correlation with soil type and pH. The downward migration of Pb and Cs is found to be due to an apparent downward movement of organic material, caused by the sedimentation of soil organic matter and subsequent turnover into CO2 . Cs migration, however, shows an additional velocity component which is found to depend on the turnover rate of soil organic matter. 相似文献