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141.
142.
Effect of an intensive mechanical removal effort on a population of non‐native rainbow trout Oncorhynchus mykiss in a South African headwater stream 下载免费PDF全文
Jeremy Shelton Olaf Weyl Johannes Van Der Walt Sean Marr Dean Impson Kristine Maciejewski Donovan Tye Helen Dallas Karen Esler 《水产资源保护:海洋与淡水生态系统》2017,27(5):1051-1055
143.
Association of genes encoding beta2 toxin and a collagen binding protein in Clostridium perfringens isolates of porcine origin 总被引:1,自引:0,他引:1
Clostridium perfringens is a cause of economically significant enteritis in livestock. Beta2 toxin, encoded by one of two cpb2 alleles, is implicated as a virulence factor in this disease. Previous studies determined that the consensus cpb2 allele is preferentially associated with C. perfringens isolated from pigs. In C. perfringens strain 13, the consensus cpb2 allele is found on the plasmid pCP13, which also carries cna, encoding a putative collagen binding protein, CpCna. This protein was shown to be a bona fide collagen adhesin, as recombinant, HIS-tagged CpCna bound collagen type I as determined by Far Western blotting. Genomic DNA from C. perfringens isolated from a variety of host species were subjected to PCR to determine the prevalence of cna in these strains and correlate its carriage with the presence and type of cpb2 allele. The cna gene was found in 55.8% of isolates from all host species (n=208) and 68.1% of porcine isolates (n=119). In cpb2+ isolates, cna was present in 69.9% of isolates from all hosts (n=153), but was found in 98.7% of porcine isolates (n=75). Furthermore in porcine isolates, the consensus cpb2 allele and cna were absolutely correlated with the presence of pcp12, a pCP13-encoded gene, and pcp12 was never found in any isolate that lacks either cpb2 allele. The finding that CpCna binds collagen and that the cna gene is associated with the consensus cpb2 allele implicates CpCna as a potential virulence factor in porcine enteritis caused by C. perfringens. 相似文献
144.
Hao Cui Zhaoguang Wang Jun Liu Yexiaoxu Wang Zhenxing Wang Jianping Fu Zhenyu Wan Ruodan Li Qingwen Li Janet Helen Fitton Yi Liu Ming Zhang 《Aquaculture Nutrition》2020,26(1):47-59
A six‐week feeding trial was conducted to determine the effects of different concentrations of fucoidan (1 g/kg, 10 g/kg and 30 g/kg; w/w) from Undaria pinnatifida on gibel carp (Carassius auratus gibelio). Our results demonstrated that 30 g/kg fucoidan significantly increased (p < .05) growth performance, intestinal digestive enzyme activities, acid phosphatase activity and immunoglobulin M content. Histological examinations revealed that gibel carp receiving 30 g/kg fucoidan had significant higher abundance of mucin‐containing goblet cells in middle and distal intestine as compared with control treatment (p < .05). Intestinal microbiota analysis showed that 30 g/kg fucoidan supplementation significantly increased (p < .05) the abundance of Cetobacterium and Aeromonas, but lowered (p < .05) the prevalence of pathogenic bacteria Plesiomonas and a mucin‐degrading bacterium Mucinivorans. Furthermore, RNA‐seq and RT‐qPCR analysis indicated that 30 g/kg fucoidan caused significant changes (p < .05) in the expression of genes involved in immune regulation (such as interleukin‐8 and cyclooxygenase), signal transduction (such as phosphatidylinositol‐4,5‐bisphosphate 3‐kinase and protein kinase B) and nutrition utilization (maltase–glucoamylase and muscarinic acetylcholine receptor 3). Together, the current study shows that fucoidan supplementation could elevate the activity of intestinal digestive enzymes, modulate intestinal microbial communities and potentiate a higher state of immune readiness, which might consequently improve growth performance and intestine health status of gibel carp. 相似文献
145.
146.
Hicks BD 《The Canadian veterinary journal. La revue veterinaire canadienne》1987,28(10):640-642
147.
Pharmacokinetic profiles of metamizole (dipyrone) active metabolites in goats and its residues in milk 下载免费PDF全文
Tae Won Kim Andrejs Sitovs Amnart Poapolathep Helen Owen Andrzej Lisowski Zulkyya Abilova Mario Giorgi 《Journal of veterinary pharmacology and therapeutics》2018,41(5):699-705
Metamizole (dipyrone, MET) is a nonopioid analgesic drug commonly used in human and veterinary medicine. The aim of this study was to assess two major active metabolites of MET, 4‐methylaminoantipyrin (MAA) and 4‐aminoantipyrin (AA), in goat plasma after intravenous (IV) and intramuscular (IM) administration. In addition, metabolite concentration in milk was monitored after IM injection. Six healthy female goats received MET at a dose of 25 mg/kg by IV and IM routes in a crossover design study. The blood and milk samples were analyzed using HPLC coupled with ultraviolet detector and the plasma vs concentration curves analyzed by a noncompartmental model. In the goat, the MET rapidly converted into MAA and the mean maximum concentration was 183.97 μg/ml (at 0.08 hr) and 51.94 μg/ml (at 0.70 hr) after IV and IM administration, respectively. The area under the curve and mean residual time values were higher in the IM than the IV administered goats. The average concentration of AA was lower than MAA in both groups. Over 1 μg/ml of MAA was found in the milk (at 48 hr) after MET IM administration. In conclusion, IM is considered to be a better administration route in terms of its complete absorption with long persistence in the plasma. However, this therapeutic option should be considered in light of the likelihood of there being milk residue. 相似文献
148.
Knut Egil Bøe Tor Gunnarson Homme Grete Helen Meisfjord Jørgensen 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2018,68(4):202-206
ABSTRACTThe aim of this study was to investigate the use and capacity of electronic feed stations (EFS) on commercial sheep farms. The study was conducted on four commercial farms and the numbers of pregnant ewes per EFS were 36, 70, 72 and 80, respectively. Each farm was visited once and behavioural observations were carried out. In addition the date and time for both entering and leaving the EFS and the amount of concentrates dispensed at each visit for extracted. The vast majority of the ewes used the EFS regularly. The number of rewarded visits per ewe per day varied from 3.2 to 5.9, whereas the number of unrewarded visits ranged from 6.0 to 21.5 per ewe per day. We conclude that feeding concentrates to groups of pregnant ewes in EFS function satisfactory, but the design of the entrance and exit gate still have to be improved considerably. 相似文献
149.
Awe was defined by Shiota, Keltner, and Mossman (2007) as an emotional human response to stimuli that overwhelm current ways of thinking and feeling as people attempt to accommodate the stimuli. For the purposes of these findings, wildlife-inspired awe was defined as the attempted accommodation of overwhelming emotional human–wildlife interactions. To explore the development of a tool for measuring wildlife-inspired awe, an online questionnaire was employed and sent to a convenience sample in which memories of wildlife-inspired awe were chronicled. Exploratory factor analysis (EFA) was used for identifying and sorting factors related to how people made sense of these memorable wildlife encounters. Three factors of wildlife-inspired awe emerged from the EFA and were labeled beauty, transcendence, and threat. The complexity of awe as an emotional response appeared to be captured within this study as awe has been framed as having both positive and negative affects. Results in the report provide insight into potential future strategies for measuring wildlife-inspired awe. 相似文献
150.
se Helen Garseth Torfinn Moldal Siri Kristine Gsnes Monika Jankowska Hjortaas Vegard Pedersen Sollien Anne‐Gerd Gjevre 《Journal of fish diseases》2019,42(3):391-396
In 2017, a PCR‐based survey for Piscine orthoreovirus‐3 (PRV‐3) was conducted in wild anadromous and non‐anadromous salmonids in Norway. In seatrout (anadromous Salmo trutta L.), the virus was present in 16.6% of the fish and in 15 of 21 investigated rivers. Four of 221 (1.8%) Atlantic salmon (Salmo salar L.) from three of 15 rivers were also PCR‐positive, with Ct‐values indicating low amounts of viral RNA. All anadromous Arctic char (Salvelinus alpinus L.) were PCR‐negative. Neither non‐anadromous trout (brown trout) nor landlocked salmon were PRV‐3 positive. Altogether, these findings suggest that in Norway PRV‐3 is more prevalent in the marine environment. In contrast, PRV‐3 is present in areas with intensive inland farming in continental Europe. PRV‐3 genome sequences from Norwegian seatrout grouped together with sequences from rainbow trout (Oncorhynchus mykiss Walbaum) in Norway and Coho salmon (Oncorhynchus kisutch Walbaum) in Chile. At present, the origin of the virus remains unknown. Nevertheless, the study highlights the value of safeguarding native fish by upholding natural and artificial barriers that hinder introduction and spread, on a local or national scale, of alien fish species and their pathogens. Accordingly, further investigations of freshwater reservoirs and interactions with farmed salmonids are warranted. 相似文献