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131.
132.
Irene Sartini Beata Łebkowska-Wieruszewska Andrzej Lisowski Amnart Poapolathep Helen Owen Mario Giorgi 《Journal of veterinary pharmacology and therapeutics》2020,43(1):26-32
The purpose of this study was two-fold: I) to determine the pharmacokinetic profile of meloxicam (MLX) in geese after intravenous (IV) and oral (PO) administration and II) to assess tissue residues in muscle, heart, liver, lung, and kidney. Ten clinically normal female Bilgorajska geese were divided into two groups (treated, n = 8; control, n = 2). Group 1 underwent a 3-phase parallel study with a 1-week washout period. In phase I, animals received MLX (0.5 mg/kg) by IV administration; the blood was collected up to 48 hr. In phases II and III geese were treated orally at the same dosage for the collection of blood and tissue samples, respectively. Group 2 served as control. After the extraction procedure, a validated HPLC method with UV detection was used for plasma and organ analysis. The plasma concentrations were quantifiable up to 24 hr after both the administrations. The elimination phase of MLX from plasma was similar in both the administration groups. The clearance was slow (0.00975 L/hr*Kg), the volume of distribution small (0.0487 L/kg), and the IV half-life was 5.06 ± 2.32 hr. The average absolute PO bioavailability was 64.2 ± 24.0%. Residues of MLX were lower than the LOQ (0.1 µg/kg) in any tested tissue and at any collection time. The dosage used in this study achieved the plasma concentration, which provides analgesia in Hispaniolan Amazon parrots for 5 out of 24 hr after PO administration. MLX tissue concentrations were below the LOD of the assay in tissue (0.03 µg/ml). A more sensitive technique might be necessary to determine likely residue concentrations in tissue. 相似文献
133.
W. C. Davis D. J. Le Tourneau Mary V. Zaehringer Helen H. Cunningham 《American Journal of Potato Research》1973,50(2):35-41
Thin slices of tissue from low (1.075–1.078 and high (1.092–1.094) specific gravity tubers were soaked in distilled water (100g/250ml) for 0, 1, 2, 3, 4, and 6 hours. Following the soak periods, the soak water and remaining tissues were separated and analyzed. Analyses of the soak water included pH, electrical conductivity, total solids, K, P and citric acid. The tissue analyses included total ash, alkalinity of ash, Ca, Mg, total N and phytate P. Other tissue samples were soaked the same periods of time and used to evaluate the amount of sloughing that occurred during cooking. All constituents studied diffused into the water during the soak periods. The greatest loss from the slices occurred in the first 2 to 3 hours and were similar for both specific gravity groups. After 6 hours the average amounts of material in the soak water were: total solids — 23%, P — 68%, phytate P —55%, K — 71%, total ash — 62%, total nitrogen — 56%, Ca — 35% and Mg —50%. Citric acid diffused into the soak water during the first 3 hours and the amount in the water then decreased. This apparent change may be due to metabolism of the acid. The sloughing of tissue decreased with the length of the soak period. The decrease in sloughing was highly correlated (P<1 %) with the length of the soak period, increases in electrical conductivity of the soak water, and with the leaching of all constituents measured. 相似文献
134.
Reproducible, objective methods which conformed closely to visual appraisal were developed for measuring color differences in either cooked or raw potatoes. Experimental samples, selected from lots stored at various temperatures for different periods of time, differed in extent of graying and yellowing. Ricing was found to be preferable to mashing as a method of cooked sample preparation. A comparison of methods of sample presentation using the Gardner Color Difference Meter showed thin-layer measurement was most successful in separating cooked samples differing in amount of yellow pigment; deep-layer measurement was most successful in separating grayed samples. A simple method for measurement of color differences in raw tubers successfully separated the samples. All three methods of sample presentation were significantly correlated with visual ranking. 相似文献
135.
Bitters Matthew E. Hicks Andrew Holtz Spencer Acruri Paulina Wilson Robert Resasco Julian Davies Kendi F. 《Landscape Ecology》2022,37(6):1483-1495
Landscape Ecology - Earth's forests are fragmented. Species' long-term persistence depends on their conservation in fragmented landscapes with remnants embedded in a matrix of human land... 相似文献
136.
Flávia E. de Mello Valéria S. Lopes-Caitar Sheila A. Xavier-Valencio Helen P. da Silva Sören Franzenburg Andreas Mehl Joseph-Alexander Verreet Maria I. Balbi-Peña Francismar C. Marcelino-Guimaraes Cláudia V. Godoy 《Plant pathology》2022,71(2):373-385
Fungicide sprays on soybean in Brazil have contributed to the selection of less sensitive isolates of Corynespora cassiicola. We collected 59 isolates of C. cassiicola from three Brazilian states and two isolates from Paraguay. We investigated their EC50 to quinone outside inhibitors (QoI) and methyl benzimidazole carbamate (MBC), any cross-resistance to compounds within QoI and MBC groups, and characterized the polymorphisms in their cytb and β-tubulin genes. Local associations of polymorphisms identified in each gene were statistically correlated with assays results. In total, 79% and 74% of the isolates were classified as resistant to QoI and MBC fungicides, respectively. There was positive cross-resistance to active ingredients within QoI and MBC groups. For QoI, all isolates presented heteroplasmy in G143A of cytb gene; the mutations F129L and G137R were not found. For MBC, 63% of isolates possessed E198A and 21% possessed F200Y mutations, associated with reduced control by MBC fungicides. Heteroplasmy was identified in two and one isolates from Brazil with E198A and F200Y mutations, respectively. The resistance factor for isolates with E198A (10.9) was statistically similar to the isolate with F200Y (8.8) mutation. Genic association analysis of the in vitro assays using discriminatory doses proved them to be accurate. Reduced sensitivity of C. cassiicola to QoI and MBC was also identified in isolates from Paraguay and resistance to QoI and MBC was widely present in C. cassiicola isolates from the main soybean-producing states in Brazil. Thus, integrated management measures should be adopted to manage soybean target spot in these countries. 相似文献
137.
Michelle T. Hulin Andrea Vadillo Dieguez Francesca Cossu Samantha Lynn Karen Russell Helen C. Neale Robert W. Jackson Dawn L. Arnold John W. Mansfield Richard J. Harrison 《Plant pathology》2022,71(4):949-965
Bacterial canker is a major disease of stone fruits and is a critical limiting factor to sweet cherry (Prunus avium) production worldwide. One important strategy for disease control is the development of resistant varieties. Partial varietal resistance in sweet cherry is discernible using shoot or whole tree inoculations; however, these quantitative differences in resistance are not evident in detached leaf assays. To identify novel sources of resistance to canker, we used a rapid leaf pathogenicity test to screen a range of wild cherry, ornamental Prunus species and sweet cherry × ornamental cherry hybrids with the canker pathogens, Pseudomonas syringae pvs syringae, morsprunorum races 1 and 2, and avii. Several Prunus accessions exhibited limited symptom development following inoculation with each of the pathogens, and this resistance extended to 16 P. syringae strains pathogenic on sweet cherry and plum. Resistance was associated with reduced bacterial multiplication after inoculation, a phenotype similar to that of commercial sweet cherry towards nonhost strains of P. syringae. Progeny resulting from a cross of a resistant ornamental species Prunus incisa with susceptible sweet cherry (P. avium) exhibited resistance indicating it is an inherited trait. Identification of accessions with resistance to the major bacterial canker pathogens is the first step towards characterizing the underlying genetic mechanisms of resistance and introducing these traits into commercial germplasm. 相似文献
138.
J. W. Plant B.V.Sc. G. P. Gard B.V.Sc. Ph.D. Helen M. Acland B.V.Sc. 《Australian veterinary journal》1976,52(6):247-249
An experiment was designed to investigate whether a condition in Australian sheep with clinical and pathological similarites to Border Disease was caused by the infection of the pregnant ewe with a Mucosal Disease virus (MDV). Forty ewes, at 58 to 63 days after mating, were inoculated with material from lambs in which all, some or none of the tissues examined contained MDV. The clinical condition was observed only in lambs born to ewes inoculated with MDV-positive material and then only to ewes in the group which had serological evidence of MDV infection. It is concluded that the Border Disease-like condition in Australian sheep is caused by the infection of the pregnant ewe with a Mucosal Disease virus. 相似文献
139.
Maria Fernanda de M. Costa Adriana K. Carmona Marcio F. M. Alves Timothy M. Ryan Helen M. Davies Garry A. Anderson Ron F. Slocombe 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(1):21-25
Angiotensin-I converting enzyme (ACE) is a key regulator of blood pressure, electrolytes and fluid homeostasis through conversion of angiotensin I into angiotensin II. Recently, a genetic polymorphism of the ACE gene, which accounts for 47% of the variation of ACE activity in blood, has been advocated as a biomarker of athletic aptitude. Different methods of analysis and determination of ACE activity in plasma have been used in human and equine research without a consensus of a "gold standard" method. Different methods have often been used interchangeably or cited as being comparable in the existing literature; however, the actual agreement between assays has not been investigated. Therefore, in this study, we evaluated the level of agreement between three different assays using equine plasma obtained from 29 horses. Two spectrophotometric assays using Furylacryloyl-phenylalanyl-glycyl-glycine as substrate and one fluorimetric assay utilizing o-aminobenzoic acid-FRK-(Dnp)P-OH were employed. The results revealed that the measurements from the different assays were not in agreement, indicating that the methods should not be used interchangeably for measurement of equine ACE activity. Rather, a single method of analysis should be adopted to achieve comparable results and critical appraisal of the literature is needed when attempting to compare results obtained from different assays. 相似文献
140.
Ana Afonso Jose Cortinas Abrahantes Franz Conraths Anouk Veldhuis Armin Elbers Helen Roberts Yves Van der Stede Estelle Méroc Kristel Gache Jane Richardson 《Preventive veterinary medicine》2014
During the Schmallenberg virus (SBV) epidemic, the European Food Safety Authority (EFSA) collected data on SBV occurrence across Europe in order to provide an assessment of spread and impact. By May 2013, twenty-nine countries were reporting to EFSA and twenty-two countries had reported cases of SBV. The total number of SBV herds reported was 13,846 and the number of SBV laboratory confirmed herds was 8730. The surveillance activities were based on the detection of SBV clinical cases (either adults or newborns). Malformation in newborns was the most commonly reported clinical sign of SBV-infection. All countries were able to provide the date when the first suspicion of SBV in the herd was reported and nineteen could report the location of the herd at a regional level. This allowed the spread of SBV in Europe to be measured both temporally and spatially. The number of SBV confirmed herds started to increase in December 2011 and two peaks were observed in 2012 (February and May). Confirmed herds continued to be reported in 2012 and into 2013. An increase during winter 2012 and spring 2013 was again observed, but the number of confirmed herds was lower than in the previous year. SBV spread rapidly throughout Europe from the initial area of detection. SBV was detected above the latitude of 60° North, which exceeds the northern expansion observed during the bluetongue virus serotype 8 epidemic in 2006–2009. The impact of SBV was calculated as ratio of the number of herds with at least one malformed SBV positive foetus and the total number of herds in this region. The 75th percentile of the malformations ratio in the various affected countries for the whole reporting period was below 1% and 3% for cattle and sheep herds, respectively. International data collection on emerging diseases represents a challenge as the nature of available data, data quality and the proportion of reported cases may vary widely between affected countries. Surveillance activities on emerging animal diseases are often structured only for case detection making the estimation of infection/diseases prevalence and the investigation of risk factors difficult. The impact of the disease must be determined to allow risk managers to take appropriate decisions. Simple within-herd impact indicators suitable for emerging disease outbreaks should be defined that could be measured as part of routine animal health surveillance programmes and allow for rapid and reliable impact assessment of emerging animal health diseases. 相似文献