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91.
    
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A cDNA encoding the subunit of thyrotropin (TSH) was isolated from a goldfish (Carassius auratus) pituitary gland cDNA library. By comparing the sequence with other teleost TSHs, a signal peptide of 19 amino acids and a mature hormone of 131 amino acids were predicted for goldfish TSH subunits. The resulting putative mature hormone of 131 amino acids had well-conserved cysteine positions and a putative N-linked glycosylation site; homology was 51–67% with TSHs from other teleosts, 38–43% with tetrapod TSHs, but only 27 and 29% with goldfish GTH-I and -II, respectively. We also examined the effects of thyroid hormones (TH) and thiourea (TU, an inhibitor of TH production) treatments on TSH and GTH subunit gene expressions in the goldfish pituitary gland. After thyroxine (T4) treatment, circulating T4 concentration increased and TSH mRNA level decreased. Supressing the amount of circulating T4 and triiodothyronine (T3) by TU treatment increased the TSH mRNA level. Moreover, T4 replacement therapy (simultaneous treatment of both TU and T4) caused a high level of circulating T4 and a low level of circulating T3, and a decrease in the TSH mRNA level. Thus, changing levels of circulating TH exert a negative feedback on the level of TSH subunit mRNA in goldfish in vivo. On the other hand, GTH subunit mRNA levels were not affected by changes in the levels of circulating TH.  相似文献   
95.
Infrared spectra of material captured from comet 81P/Wild 2 by the Stardust spacecraft reveal indigenous aliphatic hydrocarbons similar to those in interplanetary dust particles thought to be derived from comets, but with longer chain lengths than those observed in the diffuse interstellar medium. Similarly, the Stardust samples contain abundant amorphous silicates in addition to crystalline silicates such as olivine and pyroxene. The presence of crystalline silicates in Wild 2 is consistent with mixing of solar system and interstellar matter. No hydrous silicates or carbonate minerals were detected, which suggests a lack of aqueous processing of Wild 2 dust.  相似文献   
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The present study investigated the effects of lipid oxidation on quality deterioration in the ordinary and dark muscles of skipjack tuna Katuwonus pelamis during the early stages of ice storage for 72 h. The lipid hydroperoxide content of the dark muscle was significantly higher (P < 0.01) than that of the ordinary muscle throughout 72 h of ice storage. The metmyoglobin content of the ordinary muscle gradually increased, and was accompanied with darkening in the fish meat color. On the other hand, the addition of sodium ascorbate or 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox®) to the ordinary muscle of skipjack tuna significantly inhibited the formation of lipid hydroperoxide as well as metmyoglobin formation. Thus, a decrease in α-tocopherol content in the ordinary muscle with antioxidant addition was not observed during ice storage period. In conclusion, the rate of lipid oxidation of skipjack tuna ordinary muscle is closely related to metmyoglobin formation, and the addition of antioxidants to fish meat is effective at inhibiting lipid oxidation as well as myoglobin oxidation in post-mortem meat.  相似文献   
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Impact of crop rotation, fertilizer treatments and herbicidal applications on the long term maintenance of soil fertility
V. Comparative studies regarding chemical and physical factors of the yield potential of a soil over a ten year experimental period
In order to examine the influence of different crop management practices such as crop rotation, fertilizer treatments and herbicide applications on the yield potential of a soil, the Crop Science Department of the Swiss Federal Institute of Technology (ETH Zurich) conducted a long term field experiment commencing 1973. The experimental location was at the Swiss Federal Research Station for Farm Management and Agricultural Engeneering at Tanikon/TG (northeastern Switzerland).
After the ten year period, chemical and physical soil properties were not found to be substantially affected by the various experimental treatments. Organic carbon and total nitrogen were more influenced by fertilizer treatments than by crop rotation and herbicide applications. Both cation exchange capacity and pH proved to be fairly stable values.
Aggregate stability and organic matter exhibited similar profiles, although soil bulk density was affected by crop rotation. The volume of large and medium sized pores was similarly affected in that the corn-dominated crop rotation resulted in a quantifiable impact upon soil compaction.
Depending upon weed control efficiency, the grain yields of the winter wheat test crop clearly exhibited the effects of crop rotation whereas the lowest yields resulted from the corn-dominated crop rotation.  相似文献   
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Tarsal radiographs of 134 trotters were made prior to training. Radiographs were evaluated for changes commonly associated with spavin. Spavin, if present, was graded as mild, moderate, or severe. Sone of the 134 horses were radiographed annually for one, two, or three years. Radiographs of the 134 horses were compared with radiographs of 141 additional horses in which spavin had been diagnosed clinically. Only 66 of the 134 trotters (50.4%) had normal tarsal radiographs at the time of initial examination; the other 65 had some radiographic evidence of spavin. The incidence and severity of radiographic changes identified in a group of horses in which spavin was diagnosed clinically. Thus, the present results suggest that tarsal radiography is of little value in establishing the diagnosis and prognosis of spavin in trotters.  相似文献   
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