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111.
    

Purpose

Knowledge of archaeal communities is essential for understanding of the mechanism of carbon and nitrogen cycle in the mangrove sediment ecosystem. Presently, little is known about archaeal communities in the Dongzhaigang mangrove sediments. This study aimed to characterize the archaeal communities in sediments of different mangrove stands and to find out the correlations between archaeal communities and the environmental factors of sediments.

Materials and methods

Sediment samples were collected from the Dongzhaigang mangrove forest for analysis of soil properties and archaeal communities, by national standard methods and Illumina Miseq archaeal 16S ribosomal RNA (rRNA) gene sequencing, respectively.

Results and discussion

The archaeal community in the Dongzhaigang mangrove forest was constituted by some phyla from “TACK” and “DPANN” supergroups, and dominated by Euryarchaeota. Among sediments of the four mangroves in Dongzhaigang, principal coordinates analysis (PCoA) scatter plot showed a trend of difference in the archaeal community structure in the Bruguiera gymnoihiza and Kandelia candel stands from that in the Laguncularia racemosa and Sonneratia apetala stands. The abundance of the order Methanosarcinales was the highest in the sediments of K. candel mangroves, whereas the order of Methanobacteriales dominated in B. gymnoihiza sediments. The highest richness and diversity values of Archaea occurred in K. candel sediments, while the lowest in B. gymnoihiza. Pearson correlation showed the significant relationships between sediment properties and some dominant genera, with a positive and significant correlation between sediment properties and genus Methanobacterium, coinciding with the maximum values of sediment properties and abundance of Methanobacterium in the sediment of B. gymnoihiza. Such results indicated that the difference of archaeal community structure among mangrove sediments may be caused by the different sediment characteristics. Methanogenic communities in the Dongzhaigang mangrove forest sediments were, at the order level, constituted by Methanobacteriales, Methanomicrobiales, Methanosarcinales, and Methanomassiliicoccales.

Conclusions

The investigation indicated that the Dongzhaigang mangrove sediment ecosystems support diverse archaeal communities and methanogenic communities, and that there was a general trend of difference in the archaeal community structure in the B. gymnoihiza and K. candel mangrove sediments from that in the L. racemosa and S. apetala sediments. Such difference may be caused by the difference in sediment characteristics.
  相似文献   
112.
  总被引:1,自引:0,他引:1  
Soil properties related to soil development were measured in six profiles over approximately 180 years of soil development on recessional moraines of the Hailuogou Glacier, Sichuan of southwestern China. It is hypothesized that soil development is strictly time-dependent. Field and laboratory work indicate that as soil develops from initially coarse gravel outwash, the properties undergo a progressive physical and chemical change such that there is a considerable profile differentiation between the youngest and the oldest soils. The pH is also highly stratified; it decreases with time from 8.5 to 4.2 in the upper mineral soil and increases with depth in all profiles. Accumulation of soil organic C and N increases with time but the rates of accumulation become slower with time. Within the investigated timespan, soil C and N accumulated to a considerable level of 3.5 and 0.6 kg/m2 respectively, with mean annual rates of 28 g/m2 for carbon and 3.5 g/m2 for nitrogen. The rapid accumulation of organic C and N accelerates the processes of soil formation, but the content of organic C and N did not reach a steady-state during the observed timespan. The accumulation of soil organic matter results in increased acid production and in the chemical weathering of minerals that promote formation and translocation of the clay fraction and leaching of carbonate; it is also directly responsible for development of soil properties such as cation exchange capacity (CEC), and bulk density.  相似文献   
113.
    
Florfenicol (FF) is a broad-spectrum antibiotic used increasingly in aquaculture, livestock, and poultry to treat diseases. To avoid using labor-intensive instrumental methods to detect residues of FF in food and food products, a simple and convenient indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method for florfenicol's major metabolite, florfenicol amine (FFA), was developed using a polyclonal antibody prepared in this study. FFA was covalently attached to carrier protein as immunogen by using the glutaraldehyde method. The antibodies obtained were characterized by an ELISA method and showed excellent specificity and sensitivity with the 50% inhibition values (IC 50) of 3.34 microg/L for FFA in PBS buffer. In the ELISA, sample extractions were performed by ethyl acetate/ammonium hydroxide (90 + 10, v/v) following combined acid hydrolysis of FF and its known metabolites. The limits of detection (LOD) calculated from the analysis of 20 known negative swine muscle, chicken muscle, and fish samples were 3.08, 3.3, and 3.86 microg/kg (mean + 3 SD), respectively. Recoveries of FFA fortified at the levels of 5, 50, 100, and 300 microg/kg ranged from 64.6 to 124.7%, with coefficients of variation of 11.3-25.8% over the range of FFA concentrations studied. Validation of the ELISA method with FFA-fortified swine muscle at the levels of 10, 50, 100, and 200 microg/kg was carried out using GC, resulting in a similar correlation in swine muscle ( r = 0.97). The results suggest that this ELISA is a specific, accurate, and sensitive method, which is suitable for use as a screening method to detect residues of FFA in animal edible tissues.  相似文献   
114.
    
A new indirect competitive fluorescence-linked immunosorbent assay (cFLISA) method for the detection of sulfamethazine (SM2) in chicken muscle tissue was demonstrated using quantum dots (QDs) as the fluorescence label coupled with secondary antibody. The sensitivity of the cFLISA was compared with that of the HPLC method. The 50% inhibition value (IC50) and the limit of detection (LOD) of the cFLISA were 7.7 and 1.0 ng/mL, respectively. When SM2 was spiked at levels of 50, 100, and 200 ng/g, recoveries ranged from 80.6% to 117.4%, with a coefficient of variation of 6.9-9.6%. In the incurred sample analysis, the amounts of SM2 quantified by cFLISA were similar to the results obtained by the HPLC method. This study shows that cFLISA could be used as a screening method in practice.  相似文献   
115.
<正>随着石化能源的价格上涨及其所带来的环境问题,生物能源作为一种可再生能源越来越受到重视,其中燃料乙醇和生物柴油尤其被看好。目前,燃料乙醇的原料以玉米为主,生物柴油的原料以大豆和菜籽  相似文献   
116.
River floodplains have been recognised as important sinks for storing suspended sediment and associated contaminants mobilised from the upstream catchment. However, information on contemporary rates of overbank sedimentation is difficult to obtain using conventional methods. Measurements of the 137Cs content of floodplain sediments provide an alternative approach to obtaining; estimates of medium-term rates (ca. 40 years) of overbank sediment deposition. The 137Cs approach requires only a single site visit and minimum sample preparation. Furthermore, it is capable of providing information on spatial patterns of sediment deposition on floodplains, which is needed to improve our understanding of the processes involved in overbank flow and sediment deposition. This paper reviews the basis for using 137Cs measurements in floodplain sedimentation studies and presents improved procedures for interpreting 137Cs profiles in floodplain sediments and for obtaining estimates of sedimentation rates from single measurements of the total. 137Cs inventories of bulk sediment cores. The results obtained from a case study undertaken on the floodplain of the River Stour, Dorset, UK, are presented.  相似文献   
117.
Absorption of dietary anthocyanins is limited; however, fecal anthocyanin excretion has been rarely studied. We developed a method for extraction and analysis of fecal anthocyanins. Aqueous methanol (60%) maximized extraction efficiency (approximately 88%). Severe anthocyanin degradation (monitored by high-performance liquid chromatography) was observed in feces stored at -18 degrees C; therefore, storage time should be minimized and lower temperatures used. Fecal and cecal content samples were collected from 32 rats receiving either chokeberry, bilberry, grape-enriched (3.85 g monomeric anthocyanin per kg diet), or control diet for 14 weeks. Fecal anthocyanin concentrations were significantly different among groups (0.7/1.8/2.0 g/kg wet feces, chokeberry/bilberry/grape). Anthocyanin profiles of cecal contents and feces were similar. Losses in the intestinal contents were high for anthocyanin glucosides, moderate for galactosides, and negligible for arabinosides or xylosides. Acylation or diglucosylation enhanced anthocyanin stability. High anthocyanin concentration in the fecal content may favor anthocyanin absorption into the colon epithelial cells, resulting in potential health benefits.  相似文献   
118.
An aggregation index (AI) to quantify spatial patterns of landscapes   总被引:43,自引:0,他引:43  
There is often need to measure aggregation levels of spatial patterns within a single map class in landscape ecological studies. The contagion index (CI), shape index (SI), and probability of adjacency of the same class (Qi), all have certain limits when measuring aggregation of spatial patterns. We have developed an aggregation index (AI) that is class specific and independent of landscape composition. AI assumes that a class with the highest level of aggregation (AI =1) is comprised of pixels sharing the most possible edges. A class whose pixels share no edges (completely disaggregated) has the lowest level of aggregation (AI =0). AI is similar to SI and Qi, but it calculates aggregation more precisely than the latter two. We have evaluated the performance of AI under varied levels of (1) aggregation, (2) number of patches, (3) spatial resolutions, and (4) real species distribution maps at various spatial scales. AI was able to produce reasonable results under all these circumstances. Since it is class specific, it is more precise than CI, which measures overall landscape aggregation. Thus, AI provides a quantitative basis to correlate the spatial pattern of a class with a specific process. Since AI is a ratio variable, map units do not affect the calculation. It can be compared between classes from the same or different landscapes, or even the same classes from the same landscape under different resolutions.  相似文献   
119.
    
Hybridomas secreting a monoclonal antibody (mAb) against the herbicide chlorimuron-ethyl (CE) were produced by fusing the mouse myeloma cell line (SP2/0) with splenocytes from a mouse immunized against the conjugate of the sulfonamide moiety of CE and bovine serum albumin (BSA). The mAb, designated 1F5C5A10, had very weak affinity with metsulfuron, ethametsulfuron, pyrazosulfuron, bensulfuron, and chlorsulfuron. Two mAb-based indirect competitive enzyme-linked immunosorbent assays (icELISA) were developed. A conventional icELISA (icELISA-I) showed a concentration of half-maximum inhibition (IC(50)) of 11.6 ng/mL with a dynamic range of 1.6-84 ng/mL. A simplified icELISA (icELISA-II) had an IC(50) of 28.7 ng/mL and a dynamic range of 2.2-372 ng/mL. The two assays were tested on spiked water and soil samples. CE (1-500 ng/mL) fortified in water samples could be analyzed directly without any sample preparation by both immunoassays with an average recovery between 74 and 114%. icELISA-II, but not icELISA-I, was able to accurately analyze the herbicide residues in the crude soil extracts with recoveries between 99 and 129% without obvious matrix effects due to its lesser amount of sample used. In contrast to icELISA-I, icELISA-II is more convenient, whereas it consumes more reagents of coating antigen and goat anti-mouse IgG-peroxidase.  相似文献   
120.
Biomass production and nutrient (N, P, K, Ca and Mg) accumulation, distribution and cycling were quantified in young, mature and over-mature (10-, 22-, and 34-year old) Chinese fir [Cunninghamia lanceolate (Lamb.) Hook] plantations in southern China. Total stand biomass of young, mature and over-mature stands was 38, 104 and 138 t ha?1 respectively. Biomass production increased significantly with age. Stem wood represented the highest percentage of stand biomass, accounting for 41, 55 and 63 % in the young, mature and over-mature plantations respectively. Nutrients concentration was highest in live needles and branches, and lowest in stem wood. The plantations accumulated more N, followed by K, Ca, Mg, and P. Nutrient return amount, nutrient utilization efficiency, nutrient turnover time, the ratio of nutrient return and uptake increased with stand age, which implies that young Chinese fir deplete soil nutrients to maintain growth, and efficiently utilize nutrients to decrease dependence on soil nutrients as they age. Harvesting young Chinese fir plantations would therefore lead to high nutrient loss, but prolonging the rotation length could improve soil recovery, and help sustain productivity in the long-term. Improved nutrient return through litterfall as stands get older may also be beneficial to nutrient pool recovery.  相似文献   
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