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41.
Ji-young Jung Eui-hwa Nam Seol-hee Park Seung-hee Han Cheol-yong Hwang 《Journal of veterinary science (Suw?n-si, Korea)》2013,14(2):199-205
In humans, skin barrier dysfunction is thought to be responsible for enhanced penetration of allergens. Similar to conditions seen in humans, canine atopic dermatitis (CAD) is characterized by derangement of corneocytes and disorganization of intercellular lipids in the stratum corenum (SC) with decreased ceramide levels. This study was designed to evaluate the effects of a moisturizer containing ceramide on dogs with CAD. Dogs (n = 20, 3~8 years old) with mild to moderate clinical signs were recruited and applied a moisturizer containing ceramide for 4 weeks. Transepidermal water loss (TEWL), skin hydration, pruritus index for canine atopic dermatitis (PICAD) scores, and canine atopic dermatitis extent and severity index (CADESI) scores of all dogs were evaluated. Skin samples from five dogs were also examined with transmission electron microscopy (TEM) using ruthenium tetroxide. TEWL, PICAD, and CADESI values decreased (p < 0.05) and skin hydration increased dramatically over time (p < 0.05). Electron micrographs showed that the skin barrier of all five dogs was partially restored (p < 0.05). In conclusion, these results demonstrated that moisturizer containing ceramide was effective for treating skin barrier dysfunction and CAD symptoms. 相似文献
42.
国内众多专家、学者仁者见仁,从饲料企业的各个角度谈到了饲料企业执行力方面的问题,并提出了许多有针对性的建议。本篇文章,笔者根据多年对饲料企业的研究,从执行力执行的过程方面.对饲料企业指令下达的各成员要素进行分析.并对指令执行流程中的各参与要素进行初步研究.在此分析研究的基础上,从管理学角度给出一些合理化的建议,希望给相关的饲料企业在经营管理上能够有所帮助。 相似文献
43.
猪某些免疫指标及其与部分生产性状关系的研究 总被引:4,自引:1,他引:4
实验测定了哈白、杜洛克猪3月龄和5月龄的总数E-玫瑰花环形成细胞百分率、活性E-玫瑰花环形成细胞百分率、淋巴细胞转化率、血清IgG含量及5月龄接种布氏杆菌猪型二号苗后7、21、356的特异性抗体滴度,对这些免疫指标及其与部分生产性状的关系进行了分析,旨在为猪的抗病育种提供依据。实验结果表明:(1)3月龄和5月龄几个免疫指标均存在品种差异,哈白猪显著地高干杜洛克猪;(2)3月龄与5月龄间所测细胞免疫指标无显著差异,但5月龄血清IgG含量显著高于3月龄血清IgG含量;(3)哈白与杜洛克猪对布氏杆菌疫苗的抗体应答能力存在显著差异;(4)所测定的细胞免疫指标间大多表现正的相关,体液免疫指标间亦表现正的相关,而细胞免疫指标和体液免疫指标间大多具有较弱的相关;(5)所测定的几个免疫指标与生产性状间大多具有较弱的相关。 相似文献
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45.
This study was to investigate dynamic and evolution of PRRSV in a seed-stock farm by monitoring PRRSV status from 11 June 2009 to 4 August 2010. For laboratory test, around 18-24 umbilical cords from farrowed sows and 5-95 sera from nursery and grow/finish pigs were submitted around every 2 weeks interval during the study. The submitted samples were tested for PRRSV using IDEXX PRRS 2XR ELISA kit, RT-nested PCR. The PRRSV-positive samples were further sequences based on ORF5 and analyzed using MEGA 3.1 program and Beast 1.5.4 package. The surveyed farm was first infected with type II PRRSV but it was infected newly with type I PRRSV of unknown origin, showing rapid substitution to type I PRRSV as a dominant strain in 2 weeks. The type I PRRSV was first detected from umbilical cord of a farrowed sow in 12 January 2010, and secondly from nursery pigs in 26 January 2010. Although sudden increase of mean S/P ratio was found in grow/finish pigs around 2 months earlier than first type I PRRSV detection, no type I PRRSV viremia was found. Thirty three ORF5 full sequences from 14 type II to 19 type I PRRSVs were obtained chronologically in this farm and the genetic characteristics and evolution rates of those sequences were analyzed. The substitution rates (/site/day) of two types were 4.03×10(-5) (type I), 3.09×10(-5) (type II), respectively, which was more frequent than previous reports. The calculated divergence time of type I PRRSV was consistent with the time when the sudden elevation of serum IgG in grow/finish barn was first observed. This study provided fundamental data for type I PRRSV dynamic in a previously type II PRRSV-infected farm and suggested grow/finisher barn could be a primary site for PRRSV introduction. 相似文献
46.
韩占兵 《河南畜牧兽医(综合版)》2001,22(11):7-7,48
14只2岁非洲黑鸵鸟按公母比例1:2~3分成4个繁殖组合,对产蛋、受精、孵化情况进行观察分析。结果显示,每只母鸵鸟年平均产蛋量为55.2枚,最高约为73枚,最低为39枚。4个组合鸟群之间种蛋的受精率存在显著的差异P<0.01,I组的受精率最高,为59.6%;IV组的受精率最低,仅8.9%。各组的受精率、孵化率差异不显著P>0.05,平均受精蛋孵化率达到92.6%。本次受精率较低的原因为公鸟年龄小,没有完全达到性成熟。 相似文献
47.
Identification of lactic acid bacteria in the feces of dairy cows fed whole crop maize silage to assess the survival of silage bacteria in the gut 下载免费PDF全文
Hongyan Han Chao Wang Yanbing Li Zhu Yu Qingfang Xu Guangpeng Li Tang Thuy Minh Naoki Nishino 《Animal Science Journal》2018,89(1):97-104
In order to assess the survival of lactic acid bacteria (LAB) in whole crop maize silage in the gut of dairy cows, one representative silage sample and three different feces samples were collected from dairy cows on three dairy farms in Hua Bei, China and three dairy farms in Kyushu, Japan. The composition of the bacterial community was examined by denaturing gradient gel electrophoresis and quantitative polymerase chain reaction. Lactobacillus acetotolerans was detected in all bunker‐made maize silage samples, regardless of the dairy farm or sampling region from which they were sourced. A total of eight LAB species were detected in the maize silage samples, of which three (L. acetotolerans, L. pontis and L. casei) appeared to survive digestion. The populations of L. acetotolerans in silage and feces were 106–7 and 103–4 copies/g, respectively, indicating that, even for the LAB species showing potential survival in the gut, competition in this niche may be harsh and the population may substantially decrease during the digestion process. It may be difficult for silage LAB to survive in the gut of silage‐fed dairy cows, because marked decrease in population can take place during the digestion process, even for surviving species. 相似文献
48.
某规模羊场的羊布鲁氏菌病(Brucellosis,以下简称布病)阳性率高达25.16%,为了公共卫生安全和提高生产水平,自2012年4月起,采用检测+扑杀的方法,每月对该场羊群检测1次,隔离饲养可疑感染羊,淘汰阳性羊,至2013年4月,每月仍有阳性羊被检出。自2013年5月起,对该场群采用免疫+检测+扑杀的方法,至2014年后的检测中,均没有出现阳性羊,净化了本病,说明对于感染率较高的羊群,采用疫苗免疫是必要的。本文将净化的经验教训加以总结,期望为该病的净化提供参考资料。 相似文献
49.
50.
选用健康乌苏里幼公貉63只,随机分为7组(每组9只)。1~4组用以探讨适宜蛋白质水平,其蛋白质水平分别为31.54%、36.39%,40.37%和43.53%;5-7组用以探讨适宜能量水平,其能量水平分别为19589.78,21860.94和22600.04k J/kg。试验分育成前期和冬毛生长期两个阶段进行,饲喂同一营养水平的日粮。结果表明,育成前期饲喂蛋白质水平为36.39%的日粮,可获得理想的体重、体长及经济效益。 冬毛生长期,日粮蛋白质水平在31.54%~43.53%范围内,对幼貉的体重和体长无显著影响。育成期以饲喂能量水平为21860.94~22600.04 k J/kg的日粮为宜。育成前期适宜的蛋能比为16.1~16.6,冬毛生长期为14.0~14.4。 相似文献