Heme oxygenase-1 protects renal function in septic rats via influencing expression of thrombomodulin

AIM: To investigate the protective effect of heme oxygenase-1 (HO-1) on the kidney of septic rats and the influence of HO-1 on the expression of thrombomodulin (TM) in the kidney. METHODS: Sepsis in rats was developed with cecal ligation and puncture (CLP). The septic rats were randomly divided into sham group, CLP group, CLP+HO-1 inducer group and CLP+HO-1 inhibitor group (n=18). The plasma levels of creatinine (Cr), cystatin-C (Cys-C), carboxyhemoglobin (COHb), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and TM, and the changes of prothrombin time (PT) and activated partial thromboplastin time (APTT) in each group were measured. Histopathological examination was performed in the kidney. The expression of TM in the kidney tissue was detected by Western blot. RESULTS: Compared with sham group, significantly elevated plasma levels of Cr, Cys-C, TNF-α, IL-1β and TM (P<0.05), shortened PT and APTT (P<0.05), significantly increased microthrombus formation, and lowered TM expression in the kidney (P<0.05) of CLP group were observed. The administration of hemin lowered the plasma levels of Cr, Cys-C, TNF-α, IL-1β and TM (P<0.05), prolonged PT and APTT (P<0.05), attenuated microthrombus formation, and up-regulated the expression of TM in the kidney (P<0.05). In contrast, ZnPP had the opposite effects. CONCLUSION: HO-1 increases the expression of TM in the kidney and exerts anticoagulatory and antiinflammatory effects, thereby improving renal function in the septic rats.     

薄皮甜瓜新品种‘陕甜9号’

‘陕甜9号’是用‘E13’作母本,‘C20’作父本杂交育成优质抗病薄皮甜瓜新品种。植株生长势中强,果实短梨形,平均单果质量500 g,果皮光滑,黄白色,果肉浅绿至白色,中心可溶性固形物含量14.7%,耐贮运。全生育期85～100 d,果实发育期28～32 d,抗病、抗逆性较强。适应于黑龙江、河北、甘肃、新疆等地区保护地及露地栽培。     

内生菌与雷公藤细胞悬浮共培养的生理生化特性

将2株雷公藤内生真菌Fusarium oxysporum NS33与Penicillium steckii NS6、2株内生细菌Enterobacter cloacae sub sp LG3与Serratia marcescens LY1及其组合分别与雷公藤细胞悬浮共培养,对不同培养体系内雷公藤细胞的生长及其生理生化特征进行研究。结果显示,在共培养前期,与对照相比,接种单一内生菌株提高了细胞的干重,其中菌株NS6的促生效果最明显;而在共培养后期,无论是单一内生菌还是混合内生菌均对细胞生长具有抑制作用。内生真菌和菌株组合处理的培养液p H值有明显的升高,而内生细菌LY1则明显降低了培养液的p H值,其具有产酸性。另外,当雷公藤细胞同混合菌株共培养时,培养基中总糖消耗量是最大的,而接种单独菌株时则对细胞可溶性蛋白含量具有一定的提高作用。接种内生菌会影响雷公藤细胞的POD、CAT及SOD活性,与对照相比,接种单独菌株会更加提升POD与CAT的活性,而细胞MDA含量则明显下降。     

抗草甘膦栽培大豆种质挖掘及抗性生理研究

利用田间试验结合生物化学分析研究了不同的栽培大豆品系对草甘膦的抗性,试验结果显示:在草甘膦有效剂量为0.31~0.92 kg·hm~(-2)时,不同栽培大豆品种对草甘膦抗性存在明显差异,在草甘膦剂量为0.92 kg·hm~(-2)时,石豆1号存活率为100%,表现了较高的抗性,8份材料的存活率为4%~48%,表现出一定的抗性;44份材料表现敏感,存活率为0。试验研究了抗性材料石豆1号、中抗材料Williams和敏感材料中黄35对草甘膦的生理生化反应的差异,结果表明:随着草甘膦处理剂量的增加和处理时间的延长,石豆1号莽草酸含量、叶绿素含量和超氧化物歧化酶(SOD)相对活性没有明显变化。而Williams和中黄35的莽草酸含量和SOD相对活性明显升高,叶绿素含量明显降低。结果表明草甘膦(浓度0.92 kg·hm~(-2))处理下,不同抗性栽培大豆叶片中莽草酸含量有明显差异,处理5~14 d时敏感材料叶片中莽草酸含量保持较高水平,因此栽培大豆叶片莽草酸含量可以作为判断其对草甘膦抗性高低的主要生理指标之一,而SOD的活性和叶绿素含量可以作为判断栽培大豆对草甘膦抗性的辅助生理指标。     

Effect of xeroderma pigmentosum group D gene on proliferation of human umbilical arterial smooth muscle cells induced by Ox-LDL

AIM: To investigate the effects of xeroderma pigmentosum group D (XPD) gene on the proliferation of human umbilical arterial smooth muscle cells (HUASMCs) induced by oxidized low-density lipoprotein (Ox-LDL). METHODS: The recombinant plasmid pEGFP-N2/XPD was transfected into HUASMCs by liposome. The cells were divided into blank control group, pEGFP-N2 group, pEGFP-N2/XPD group, Ox-LDL group, Ox-LDL+pEGFP-N2 group and Ox-LDL+pEGFP-N2/XPD group. The proliferation rate of the cells was detected by MTT and EdU assays. The apoptotic rate and cell cycle distribution were analyzed by flow cytometry. The protein levels of XPD, caspase-3, Bcl-2 and Bax were determined by Western blot. RESULTS: Compared with blank control group, the expression of XPD was increased in pEGFP-N2/XPD group (P<0.05). According to the results of MTT and EdU assays, the cell proliferation in pEGFP-N2/XPD group was reduced compared with blank control group (P<0.05). Compared with Ox-LDL group, the cell proliferation in Ox-LDL+pEGFP-N2/XPD group was significantly inhibited (P<0.05). According to the results of flow cytometry, the cell proportion of S phase decreased and the G₀/G₁-phase cell proportion increased significantly in pEGFP-N2/XPD group and Ox-LDL+pEGFP-N2/XPD group compared with blank control group and Ox-LDL group, repectively (P<0.05). Compared with blank control group and Ox-LDL group, the protein level of Bcl-2 decreased and the protein levels of Bax and cleaved caspase-3 increased in pEGFP-N2/XPD group and Ox-LDL+pEGFP-N2/XPD group, respectively (P<0.05). CONCLUSION: XPD inhibits the proliferation of HUASMCs and promotes their apoptosis, and reduces the promoting effect of Ox-LDL on the proliferation of HUVSMCs. XPD may be the target for treatment of atherosclerosis.     

引黄灌区不同肥料类型和施肥技术对稻田氮磷流失的影响

为减少引黄灌区稻田退水导致的农业面源污染,寻找水稻稳产和环境友好的最佳结合点,研究了大田示范条件下不同肥料类型和施肥技术对稻田田面水和排水沟退水氮磷变化特征的影响。结果表明,与常规施肥处理比较,优化施肥和侧条施肥均可以显著降低稻田田面水和排水沟中TN质量浓度,其中以侧条施肥处理效果最好,田面水和排水沟中TN质量浓度仅为常规施肥的47.4%和34.3%,各施肥处理间田面水和排水沟中TP质量浓度变化差异不显著。优化施肥和侧条施肥处理在肥料减量的条件下水稻产量并没有降低,其中有机种植处理产量最低,相对产量仅为常规处理的73.5%。综合考虑水稻产量和环境因素,优化施肥和侧条施肥技术可以作为环境友好型施肥技术在引黄灌区进行推广。     

优质稻中浙优8号在惠安县种植表现及高产栽培技术

中浙优8号是中国水稻研究所和浙江勿忘农种业有限公司选育的优质高产晚籼杂交稻,2013年通过福建省引种认定。2015—2016年引进惠安县黄塘镇种植,表现生长整齐,分蘖力强,穗大粒多,产量高,米质优,适应性好,抗病性强,适合当地种植推广。总结了中浙优8号在惠安县种植表现及高产栽培技术。     

保水剂对千里光种子萌发及幼苗生长的影响研究

为探明保水剂对千里光(Senecio scandens)种子萌发、幼苗生长的影响以及最佳使用量,采用室内盆栽试验,研究了5个梯度的保水剂浓度[0(CK)、0.10%、0.20%、0.30%、0.40%]对千里光种子萌发及幼苗生长的影响。试验结果表明,添加适宜浓度的保水剂可提高千里光种子发芽率,最佳保水剂浓度为0.30%,较对照提高了7.62%。添加保水剂可以提升千里光幼苗生物量、株高与根系长度,株高随保水剂浓度增加呈先增加后下降趋势,根系长度随保水剂浓度增加而增加,在0.40%浓度处理达到最大值。在采用千里光进行绿化时建议加入浓度为0.30%的保水剂,有利于提高其成活率及绿化效果。     

淮河水系沙塘鳢形态特征和分类地位初步研究

研究分析了淮河水系沙塘鳢(Odontobutis obscura)的形态特征,并对其分类地位进行了探讨。可数性状:背鳍Ⅶ,Ⅰ-9~10,尾鳍14~15,臀鳍Ⅰ-7~8,腹鳍Ⅰ-5,胸鳍14~15,纵列鳞36~41,横列鳞14~18,体侧感觉乳突线鳞28~30,第一鳃弓鳃耙数9~11,脊椎骨数28~29。可量性状:全长是体长(1.23±0.03)倍;体长是体高(3.82±0.45)倍,是头长(3.08±0.49)倍,是尾柄长(5.19±0.54)倍,是消化道长(1.86±0.52)倍,是肠长(6.99±2.86)倍;头长是吻长(2.94±0.42)倍,是眼径(6.09±0.87)倍,是眼间距(3.86±0.61)倍,是口裂宽(1.94±0.27)倍;体高是体厚(0.96±0.11)倍;尾柄长是尾柄高(1.66±0.18)倍。     

有机物料添加对内蒙古河套灌区碱化土壤可溶性有机碳的影响

通过研究不同有机物料添加对内蒙古河套灌区碱化土壤可溶性有机碳含量变化的影响,寻找合理高效的增碳方式,降低碱化土壤有机碳损失。以内蒙古河套灌区轻度、中度盐碱土为对象进行田间试验,设置生物炭（BC）添加、羊粪（GM）添加,对照（CK）3个处理,对比和分析不同有机物料添加后土壤有机碳（SOC）、可溶性有机碳（DOC）、土壤理化性质的变化。结果表明：（1）与CK相比,轻度碱化土壤BC、GM处理土壤DOC含量分别增加3.28%,20.66%,SOC含量增加5.40%,10.30%,中度碱化土壤BC和GM处理下可溶性碳增加41.32%,74.10%,土壤SOC含量增加60.24%,79.16%;（2）轻度碱化土壤BC和GM处理土壤DOC含量与SOC含量呈负相关,中度碱化土壤BC和GM处理下呈正相关;（3）盐碱土壤SOC、DOC含量主要与土壤pH、电导率的变化有关;BC处理较GM处理相比土壤电导率低约1.93%~29.15%,而GM处理土壤pH、碱化度比BC处理低0.31%~1.53%,7.10%~24.63%。总体来看,有机物料添加后均能使土壤中SOC、DOC含量提高,不同程度地降低土壤碱化程度,且羊粪添加较生物炭略好,因此羊粪添加对河套灌区碱化土壤碳含量的提高比生物炭添加更有效,土壤改良效果更好,土壤理化性质改善更明显。