对三农问题和农村城市化问题的若干思考

中国是一个农业大国，农业经济状况在很大程度上决定了整体经济的发展态势。对中国当前农业发展现状中的主要问题进行分析，认为只有积极进行城市化建设才能解决中国农业发展的根本问题，同时，就中国城市化的路径选择与城市化发展的中短期目标提出自己的看法。     

机组段运转特性曲线的绘制及其应用

机组段是水电站运行的基本单元。从运行的需要出发,采用机组段运转特性曲线来指导运行,比用水轮机运转特性曲线方便。本文介绍机组段运转特性曲线的绘制方法及其在水电站运行中的作用。     

棱锥型孔排种部件的研究

根据地膜覆盖精量播种玉米的要求，研制了棱锥型孔排种部件，介绍了它的工作原理及基本参数，经台架试验得出了该排种器结构参数的较优设计方案。在较优设计参数下，棱锥型孔排种圆盘的线速度ｖ＝０．８０７ｍ／ｓ时，单粒率为９３．６７％，空穴率为１．６７％，其指标达到了机械式膜上玉米精量播种的先进水平，为地膜覆盖播种玉米提供了一种机械式精量排种器。     

水牛脾切除术的研究

近3年来成功地作了20例水牛的脾切除术,没有1例因手术而招致死亡。成功地探索出适合于水牛脾切除术的麻醉方法、切口部位和大小、手术操作方法。     

A Novel Gelatinase from Marine Flocculibacter collagenilyticus SM1988: Characterization and Potential Application in Collagen Oligopeptide-Rich Hydrolysate Preparation

Although the S8 family in the MEROPS database contains many peptidases, only a few S8 peptidases have been applied in the preparation of bioactive oligopeptides. Bovine bone collagen is a good source for preparing collagen oligopeptides, but has been so far rarely applied in collagen peptide preparation. Here, we characterized a novel S8 gelatinase, Aa2_1884, from marine bacterium Flocculibacter collagenilyticus SM1988^T, and evaluated its potential application in the preparation of collagen oligopeptides from bovine bone collagen. Aa2_1884 is a multimodular S8 peptidase with a distinct domain architecture from other reported peptidases. The recombinant Aa2_1884 over-expressed in Escherichia coli showed high activity toward gelatin and denatured collagens, but no activity toward natural collagens, indicating that Aa2_1884 is a gelatinase. To evaluate the potential of Aa2_1884 in the preparation of collagen oligopeptides from bovine bone collagen, three enzymatic hydrolysis parameters, hydrolysis temperature, hydrolysis time and enzyme-substrate ratio (E/S), were optimized by single factor experiments, and the optimal hydrolysis conditions were determined to be reaction at 60 ℃ for 3 h with an E/S of 400 U/g. Under these conditions, the hydrolysis efficiency of bovine bone collagen by Aa2_1884 reached 95.3%. The resultant hydrolysate contained 97.8% peptides, in which peptides with a molecular weight lower than 1000 Da and 500 Da accounted for 55.1% and 39.5%, respectively, indicating that the hydrolysate was rich in oligopeptides. These results indicate that Aa2_1884 likely has a promising potential application in the preparation of collagen oligopeptide-rich hydrolysate from bovine bone collagen, which may provide a feasible way for the high-value utilization of bovine bone collagen.     

塑料薄膜培养单细胞藻技术

单细胞藻是水域中最主要的初级生产者,具有营养丰富,个体小,不败坏水质的优点.它是水产育苗中不可缺少的饵料生物,尤其在贝类育苗中对藻类需求尤为突出.2005年6～8月份,在丰南渤海资源增殖站利用塑料袋薄膜培养单细胞藻取得成功.现将其技术要点概述如下.     

第三次全国农作物种质资源调查与收集行动湖南茶树专项总结报告

2015年10月至2018年12月,项目组参与第三次全国农作物种质资源调查与收集行动,对湖南省茶树种质资源进行普查、调查和征集、收集,共普查、调查和征集、收集茶树种质资源165份,涉及45个农业县(市、区),范围为东经109.176821°~114.005865°、北纬24.943720°~29.819774°、海拔31.33~1469.00m,并对湖南茶树种质资源的保护和合理开发利用提出了建议。