Prevalence of Campylobacter and four endoparasites in dog populations associated with Hearing Dogs

OBJECTIVES: This study established the prevalence of four gastrointestinal parasites (Isopora species, Giardia species, Uncinaria stenocephala, Toxocara canis) and one bacterial infection (Campylobacter jejuni/coli) in dogs associated with the charity Hearing Dogs for Deaf People. METHODS: Dogs' faeces were routinely sampled from dogs according to whether they were in Socialising, Kennelling or Visiting the Hearing Dogs site. A further group consisted of dogs with diarrhoea. RESULTS: Prevalence rates for dogs in Socialising (n=326), Kennelling (n=117), Visiting (n=106) and Diarrhoea (n=59) groups, respectively, were as follows: Campylobacter- 26, 21, 15 and 31 per cent; Coccidia - 3, 0, 0 and 2 per cent; Giardia- 13, 3, 2 and 10 per cent; hookworm - 1, 1, 4 and 5 per cent; and Toxocara- 4, 2, 3 and 2 per cent. There were significant differences in the levels of Giardia and hookworm found between the four different groups of dogs. No significant gender differences were found, and dogs that were positive for Campylobacter in the Visiting group were significantly younger. CLINICAL SIGNIFICANCE: This study provides current information on the infection rates in specific dog populations in the UK. This is relevant to the veterinary health of dogs and the possible risk of zoonotic infection to humans.     

Black pod: diverse pathogens with a global impact on cocoa yield

ABSTRACT Pathogens of the Straminipile genus Phytophthora cause significant disease losses to global cocoa production. P. megakarya causes significant pod rot and losses due to canker in West Africa, whereas P. capsici and P. citrophthora cause pod rots in Central and South America. The global and highly damaging P. palmivora attacks all parts of the cocoa tree at all stages of the growing cycle. This pathogen causes 20 to 30% pod losses through black pod rot, and kills up to 10% of trees annually through stem cankers. P. palmivora has a complex disease cycle involving several sources of primary inoculum and several modes of dissemination of secondary inoculum. This results in explosive epidemics during favorable environmental conditions. The spread of regional pathogens must be prevented by effective quarantine barriers. Resistance to all these Phytophthora species is typically low in commercial cocoa genotypes. Disease losses can be reduced through integrated management practices that include pruning and shade management, leaf mulching, regular and complete harvesting, sanitation and pod case disposal, appropriate fertilizer application and targeted fungicide use. Packaging these options to improve uptake by smallholders presents a major challenge for the industry.     

Freezing tolerance of conifer seeds and germinants

Survival after freezing was measured for seeds and germinants of four seedlots each of interior spruce (Picea glauca x engelmannii complex), lodgepole pine (Pinus contorta Dougl. ex Loud.), Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) and western red cedar (Thuja plicata Donn ex D. Donn). Effects of eight seed treatments on post-freezing survival of seeds and germinants were tested: dry, imbibed and stratified seed, and seed placed in a growth chamber for 2, 5, 10, 15, 20 or 30 days in a 16-h photoperiod and a 22/17 degrees C thermoperiod. Survival was related to the water content of seeds and germinants, germination rate and seedlot origin. After freezing for 3 h at -196 degrees C, dry seed of most seedlots of interior spruce, Douglas-fir and western red cedar had 84-96% germination, whereas lodgepole pine seedlots had 53-82% germination. Freezing tolerance declined significantly after imbibition in lodgepole pine, Douglas-fir and interior spruce seed (western red cedar was not tested), and mean LT50 of imbibed seed of these species was -30, -24.5 and -20 degrees C, respectively. Freezing tolerance continued to decline to a minimum LT50 of -4 to -7 degrees C after 10 days in a growth chamber for interior spruce, Douglas-fir and lodgepole pine, or after 15 days for western red cedar. Minimum freezing tolerance was reached at the stage of rapid hypocotyl elongation. In all species, a slight increase in freezing tolerance of germinants was observed once cotyledons emerged from the seed coat. The decrease in freezing tolerance during the transition from dry to germinating seed correlated with increases in seed water content. Changes in freezing tolerance between 10 and 30 days in the growth chamber were not correlated with seedling water content. Within a species, seedlots differed significantly in freezing tolerance after 2 or 5 days in the growth chamber. Because all seedlots of interior spruce and lodgepole pine germinated quickly, there was no correlation between seedlot hardiness and rate of germination. Germination rate and freezing tolerance of Douglas-fir and western red cedar seedlots was negatively correlated. There was a significant correlation between LT50 after 10 days in the growth chamber and minimum spring temperature at the location of seedlot origin for interior spruce and three seedlots of western red cedar, but no relationship was apparent for lodgepole pine and Douglas-fir.     

Soil and sediment quality and composition as factors in the distribution of damage at the December 26, 2003, Bam area earthquake in SE Iran (M s = 6.6)

Background, aim, and scope

The rapid growth of the world’s population over the past few decades has led to a concentration of people, buildings, and infrastructure in urban areas. The tendency of urban areas to develop in sedimentary valleys has increased their vulnerability to earthquakes due to the presence of soft soil and sediment. Several earthquakes have clearly demonstrated that local soil and sediment conditions can have a significant influence on earthquake-induced ground motion and damage pattern, respectively. Many studies confirm the relationship between site effect and ground motion (Borcherdt in Bull Seismol Soc Am 60:29–61, 1970; Bouckovalas et al. in Geotech Geolog Eng (Historical Archive) 14(2):111–128, 1996; Fäh et al. in Seismology 1:87–10, 1997; Atakan et al. in Nat Hazards 15(2–3):139–164, 1997; Christaras et al. in Geodynamics 26(2–4):393–411, 1998; Raptakis et al. in Bull Earthquake Eng 2(3):285–301, 2004a; Raptakis et al. in Soil Dyn Earthq Eng 25:871–887, 2005; Marka et al. in Pure Appl Geophys 158:2349–2367, 2001; Marka et al. in Soil Dyn Earthq Eng 25(4):303–315, 2005; Importa et al. in Seismology 9(2):191–210, 2005; Tyagunov et al. in Nat Hazards 38:199–214, 2006; Lombardo et al. in Nat Hazards 38:339–354, 2006; Rayhani et al. in Geotech Geol Eng 21(1):91–100, 2008). In order to classify the suitability of the soil and subsurface sediment units for urban planning and compare their mechanical behavior with the non-uniform damage observed in the 2003 earthquake, we performed some geotechnical and geophysical analyses of soil and sediment samples collected from different locations in Bam City.

Methodology

Geophysical and geotechnical properties, such as grain size distribution, sorting, plasticity, Poison’s ratio, shear strength, compression index, permeability, and P and S wave velocities in soil and subsurface sediments, were measured. Maps (in GIS environment) and cross-sections were prepared for the study area.

Results

According to our observations, a great number of buildings were damaged in areas of the city where silty and clayey soils dominate, presenting very low permeability, low wave velocity together with high plasticity, and compressibility. In the study area, we recognized eight sediment types. Shear wave propagation velocities allowed for the identification of four seismic layers referred to as the surface layer, second layer, and third layer and seismic bedrock. We found that the damages observed in the Bam area were related to the physical and mechanical properties of the soil and subsurface sediment units. We also found that the soil thickness that was estimated by geophysical surveying shows a direct relationship with damage rate observations. Furthermore, we observed that landslide and qanat collapses have occurred in some areas where sand and silty sand soils and subsurface sediments dominate.

Discussion

The distribution of the damage shows a microzonation that is very serious in some points in the city along the main fault, especially where it is located on thick, fine, medium, and loose soil and sediments. In general, there is a discernable west to east increase in the damage across the city. The average level of destruction for the entire city was ~75%, while the eastern part of the city locally reached 100% destruction level. The major factors that influenced the damage and destruction in the Bam region were the distance of a given site from the seismic source, the quality of foundation soil and subsurface sediment, and the type of building. The Bam earthquake occurred on a single fault network comprising the Bam and Arg-e-Bam faults (Funning et al. in J Geophys Res 100(B09406):1–23, 2005). The sediments and soil of the area (unconsolidated silty sand and sandy gravel) belong to braided fluvial and alluvial facies. Most of the buildings near the epicenter area were old and constructed of mud bricks using mud cement.

Recommendations and perspectives

A combined sedimentological, geological, neotectonic, geotechnical, paleoseismological, and geophysical investigation in urban areas (especially in alluvial valleys) will give the detailed knowledge of the subsurface structure required for the accurate and precise seismic hazard assessments needed for effective earthquake protection planning. This paper shows that for the Bam situation, sedimentological data are required to provide an interpretive context for the geophysical data.     

我国禽用活疫苗生产SPF化势在必行

改革开放以来,随着我国畜牧业持续、健康和稳定的发展,畜产品质量与产量显著提高.据统计,2006年畜牧业总产值约1.4万亿元,占农业总产值的34%.肉、蛋、奶产量分别达到7 980、2 940和3 290万吨,比2000年分别增长58.8%、25.8%和193%,我国肉、蛋总产量已名列世界首位,奶产量跃居世界第五位.畜牧业的成就与我国畜禽疫病防治水平的提高密不可分,由于我国养殖业的生物安全管理比较差,主要靠疫苗免疫接种防控畜禽疫病.免疫接种安全、高效的疫苗是防控家禽疫病的关键,而实行禽用活疫苗的SPF化生产是保证疫苗纯净安全、有效的重要手段.……