Plasma lipoprotein concentration as an indicator of fatness in broilers: Development and use of a simple assay for plasma very low density lipoproteins

1. A turbidimetric assay for plasma very low density lipoproteins (VLDL) was developed, based on their selective precipitation with heparin and Mg²⁺.

2. Measurements of plasma VLDL concentration using the turbidimetric method showed a correlation of 0–98 with plasma VLDL plus low density lipoprotein triglyceride concentration estimated chemically.

3. Correlations between plasma VLDL concentration measured turbidimetrically and body fat content were similar to or higher than those obtained previously using a chemical assay. Correlations for broiler grandparent stock fed on a low‐fat diet were 0.70 and 0.65 for males and females respectively.

4. Turbidimetric measurement of plasma VLDL concentration provides a simple, rapid and effective method of estimating fatness in live broilers that could be applied to commercial broiler breeding programmes.     

The response of cage‐reared broiler cockerels to dietary supplements of nitrovin,zinc bacitracin or penicillin used singly or in paired combinations

1. The effect of feeding diets supplemented with procaine penicillin (50 mg/kg), zinc bacitracin (25 mg/kg) or nitrovin (20 mg/kg) singly or in combinations on the performance of broiler cockerels from 1 to 56 d was assessed.

2. The growth of treated birds was significantly better than that of controls fed on a basal diet, but no combination of additives was significantly superior to the additives given singly.

3. Performance up to 56 d of birds fed continuously on a diet supplemented with zinc bacitracin (25 mg/kg) from 1 d old was better than that obtained when the supplement was either introduced or withdrawn at different ages.     

Herbicide Effects on Sugarcane Growth, Pythium Root Rot, and Pythium arrhenomanes

ABSTRACT Six herbicides were evaluated for their effects on Pythium root rot and growth of sugarcane in greenhouse experiments and on in vitro mycelial growth rate of Pythium arrhenomanes. Pendimethalin and atrazine were most inhibitory to mycelial growth, but neither reduced root rot severity. Asulam, atrazine, and metribuzin were not phytotoxic to sugarcane and did not affect root rot symptom severity in clay loam or silt loam field soils. Atrazine and metribuzin increased shoot number, and atrazine increased total shoot weight for treated plants in silt loam soil. Glyphosate, pendimethalin, and terbacil were phytotoxic to sugarcane. These herbicides increased root rot severity, but the extent to which growth reductions resulted from increased disease severity or from direct herbicide injury was not clear. Adverse effects on plant growth and root rot severity were greater in clay loam than in silt loam soil. The results suggest that sugarcane injury from some herbicides is compounded by increased severity of root rot.     

Comparison of Edwardsiella ictaluri isolates from different hosts and geographic origins

The intraspecific variability of E. ictaluri isolates from different origins was investigated. Isolates were recovered from farm‐raised catfish (Ictalurus punctatus) in Mississippi, USA, tilapia (Oreochromis niloticus) cultured in the Western Hemisphere and zebrafish (Danio rerio) propagated in Florida, USA. These isolates were phenotypically homologous and antimicrobial profiles were largely similar. Genetically, isolates possessed differences that could be exploited by repetitive‐sequence‐mediated PCR and gyrB sequence, which identified three distinct E. ictaluri genotypes: one associated with catfish, one from tilapia and a third from zebrafish. Plasmid profiles were also group specific and correlated with rep‐PCR and gyrB sequences. The catfish isolates possessed profiles typical of those described for E. ictaluri isolates; however, plasmids from the zebrafish and tilapia isolates differed in both composition and arrangement. Furthermore, some zebrafish and tilapia isolates were PCR negative for several E. ictaluri virulence factors. Isolates were serologically heterogenous, as serum from a channel catfish exposed to a catfish isolate had reduced antibody activity to tilapia and zebrafish isolates. This work identifies three genetically distinct strains of E. ictaluri from different origins using rep‐PCR, 16S, gyrB and plasmid sequencing, in addition to antimicrobial and serological profiling.     

Soil water in relation to irrigation, water uptake and potato yield in a humid climate

Efficiently controlling soil water content with irrigation is essential for water conservation and often improves potato yield. Volumetric soil water content (θ_v) in relation to irrigation, plant uptake, and yield in potato hills and replicated plots was studied to evaluate four water management options. Measurements of θ_v using a hammer driven probe were used to derive a θ_v index representing the relative θ_v status of replicated plots positioned along a hill slope. Time series for θ_v were determined using time domain reflectometry (TDR) probes at 5 and 15 cm depths at the center, shoulder, and furrow locations in potato hills. Sap flow was determined using flow collars in replicated field plots for four treatments: un-irrigated, sprinkler, surface drip, and sub-surface drip irrigation (40 cm depth). Irrigated yields were high/low as the θ_v index was low/high suggesting θ_v excess was a production problem in the wetter portions of the study area. The diurnal pattern of sap flow was reflected in the θ_v fluctuation it induces at hill locations with appreciable uptake. Hill locations with higher plant uptake were drier as was the case for the 5 cm (dry) depth relative to the 15 cm (wet) depth and for locations in the hill (dry) relative to the furrow (wet). The surface drip system had the lowest water use requirement because it delivers water directly to the hill locations where uptake is greatest. The sub-surface drip system wetted the hill gradually (1-2 days). Measurement of the θ_v index prior to experimental establishment could improve future experimental design for treatment comparisons.     

Spatial econometric analysis of a field-scale site-specific nitrogen fertilizer experiment on wheat (Triticum aestuvum L.) yield and quality

Knowledge of site-specific response may help farmers to tailor their management decisions with the help of precision farming technologies. However, farmers often have only a vague idea of the economic potential for site-specific management of their fields, which is important for investment decisions on precision farming technologies. This study presents an on-farm experimental approach to identify the economic potential of site-specific fertilization strategies at low costs. A strip trial with winter wheat (Triticum aestivum, L.) was established with precision farming technologies. Twelve different nitrogen fertilizer rates split in two applications were applied to 30 plots over a total strip length of 1.5 km. Geo-referenced yield was recorded with the harvester. Furthermore, electrical conductivity of the soil was measured and grain quality was surveyed with hand selected samples. With the help of advanced spatial statistical methods, within-field site-specific response was modeled with sufficient accuracy at comparably low costs. Electrical conductivity of the soil, elevation above sea level, and derivates of a digital elevation model were used as covariates to identify a possible economic potential for site-specific fertilization. Yield and protein response was best predicted with spatially adjusted regression models with site-characteristics or their interaction with management variables. Protein content was essential for achieving best economic results. The economic potential for site-specific fertilization strategies for the analyzed field was below 2 €/ha. However, the approach to identify the potential may be transferred to other locations with greater potential for site-specific farming.     

Reduced fertility in triploids of <Emphasis Type="Italic">Acacia auriculiformis</Emphasis> and its hybrid with <Emphasis Type="Italic">A. mangium</Emphasis>

Polyploid breeding offers the possibility of increased variability in the search for improved growth, site adaptation and disease resistance in tropical acacias. A key focus of breeding in Vietnam has been the production and testing of vigorous triploid clones which are expected to have the added advantage of being sterile. Triploids obtained by manual crosses between diploid and tetraploid trees or by bulk screening of seedlings derived from open-pollinated seedlots were verified using flow cytometry. Thirteen clones are under field testing for growth rate, tree form and fertility. Six of these are now reproductively mature and flowered as prolifically as diploids. However no pollen germinated, either on agar or on their own stigmas under controlled pollination. Only one clone (X01—F₁ hybrid of tetraploid A. mangium and diploid A. auriculiformis) produced open pollinated pods on 0.05% of hermaphrodite flowers and these contained an average of 1.3 filled seeds per pod, about one-fifth the number observed in diploids. Less than 25% of germinated progeny from this triploid clone survived at 3 months after sowing and survivors were severely stunted with arrested growth. Ploidy and genotype analysis revealed them to be predominantly aneuploids, ranging from hyperdiploid to hypotetraploid with 95% being selfs. We are confident that we can select triploid clones which will be effectively infertile if deployed in plantations and may be of particular value in situations where the normal prolific natural regeneration of tropical acacias is highly undesirable.