Mobile terrestrial laser scanner applications in precision fruticulture/horticulture and tools to extract information from canopy point clouds

LiDAR sensors are widely used in many areas and, in recent years, that includes agricultural tasks. In this work, a self-developed mobile terrestrial laser scanner based on a 2D light detection and ranging (LiDAR) sensor was used to scan an intensive olive orchard, and different algorithms were developed to estimate canopy volume. Canopy volume estimations derived from LiDAR sensor readings were compared to conventional estimations used in fruticulture/horticulture research and the results prove that they are equivalent with coefficients of correlation ranging from r = 0.56 to r = 0.82 depending on the algorithms used. Additionally, tools related to analysis of point cloud data from the LiDAR-based system are proposed to extract further geometrical and structural information from tree row crop canopies to be offered to farmers and technical advisors as digital raster maps. Having high spatial resolution information on canopy geometry (i.e., height, width and volume) and on canopy structure (i.e., light penetrability, leafiness and porosity) may result in better orchard management decisions. Easily obtainable, reliable information on canopy geometry and structure may favour the development of decision support systems either for irrigation, fertilization or canopy management, as well as for variable rate application of agricultural inputs in the framework of precision fruticulture/horticulture.     

How useful is plastination in learning anatomy?

In recent years plastination has begun to revolutionize the way in which human and veterinary gross anatomy can be presented to students. The study reported here assessed the efficacy of plastinated organs as teaching resources in an innovative anatomy teaching/learning system. The main objective was to evaluate whether the use of plastinated organs improves the quality of teaching and learning of anatomy. For this purpose, we used an interdepartmental approach involving the departments of Veterinary Anatomy, Human Anatomy, Veterinary Surgery, and Education Development and Research Methods. The knowledge base of control and experimental student groups was examined before and after use of the fixed or plastinated resources, respectively, to gather information evaluating the effectiveness of these teaching resources. Significant differences (p < 0.001) between control and experimental groups of Human and Veterinary Anatomy were observed in the post-test results. The Veterinary Surgery students had the most positive opinion of the use of plastinated specimens. Using these data, we were able to quantitatively characterize the use of plastinated specimens as anatomy teaching resources. This analysis showed that all the plastinated resources available were heavily used and deemed useful by students. Although the properties of plastinated specimens accommodate student needs at various levels, traditional material should be used in conjunction with plastinated resources.     

Adapting a model for even-aged Pinus pinea L. stands to complex multi-aged structures

Stone pine (Pinus pinea L.) stands have been usually managed as even-aged stands. Main objectives in management combine two main commercial productions, timber and pine nuts with other social aspects: soil protection, recreational use and biodiversity conservation. Multifunctional management, together with the occurrence of successive events affecting regeneration have oriented managers to propose a management schedule based on the establishment and preservation of a low-stocking multi-aged complex structure on favourable locations. Despite the recent effort on modelling growth and yield on even-aged stands of stone pine, no studies focusing on modelling dynamics for uneven-aged stands have yet been developed up to present.In this study, a proposal is presented for adapting and calibrating an existing tree-level model, originally developed for even-aged stands of stone pine (model PINEA2), to multi-aged complex stands. Data from four multi-aged trials and 61 plots from the National Forest Inventory were used to adapt the whole set of functions included in the original model. In our study, four different methods have been proposed to adapt the original equations: (1) direct validation and re-parameterization; (2) size class modelling; (3) refit of functions after removing typical even-aged covariates; and (4) multilevel calibration. Adaptation is based on assuming that a multi-aged stand of stone pine can be seen as the sum of independent, smaller, even-aged groups. The low densities of the stands, the early liberation of the most vigorous trees in all size classes and the major importance of root-level competition for water in Mediterranean forests are the main factors explaining these particular dynamics. Results show the suitability of the proposed method, attaining unbiased estimates with a degree of accuracy similar to that achieved in applying the original model to even-aged stands. The adapted model (PINEA_IRR) constitutes a flexible tool for the management and maintenance of stone pine stands, covering a wide range of within stand structural complexity, including forests in transition.     

Base-age invariant cork growth model for Spanish cork oak (<Emphasis Type="Italic">Quercus suber</Emphasis> L.) forests

A cork growth model for Spanish cork oak forests was developed using data from 432 cork samples. Ten dynamic equations derived from the generalised algebraic difference approach (GADA) were considered for analysis, and both numerical and graphical methods were used to compare alternative models. All of the equations are base-age invariant and directly estimate accumulated cork thickness in complete years at any moment of the cork rotation. The fittings were done using the nested iterative method of the stochastic regression approach. The GADA formulation derived by Krumland and Eng (2005) from the Richards model by considering a ₁ and a ₃ as related to site productivity was finally selected. The cork growth model developed in this study allows the total accumulated cork thickness in complete years to be estimated in the fourth year of cork rotation with close to 80% reliability for any debarking period, increasing to nearly 90% from the eighth year onwards.     

A Critical Overview of Forestry Seedling Production Policies and Practices in Relation to Smallholder Forestry in Developing Countries

This paper reviews forestry seedling production systems in South-east and East Asia and identifies problems with respect to seedling quality, seedling distribution and financial sustainability, and measures which have been adopted or advocated to improve performance in this sector. The paper draws in particular on experience in a series of research projects on smallholder forestry in the Philippines. Some observations are also drawn from the following papers in this combined special issue of Small-scale Forestry. It is found that a mix of public and private sector models are adopted for forestry seedling production, between and even within countries. Often nurseries are set up to provide seedlings for a government-directed expansion in tree planting, and have difficulty surviving once the initial planting purpose is completed. Private nurseries often lack resources, and depend on contracts to supply seedlings for financial viability. Demand tends to be highest for fast-growing species (often exotics), fruit trees, and ornamentals in the case of urban nurseries. Government policies typically favour quantity over quality of the seedlings produced. Considerable scope exists for adopting best or at least improved management practice in seedling nurseries.     

Enhancing Tree Seedling Supply to Smallholders in Leyte Province, Philippines: An Evaluation of the Production System of Government Nursery Sector and Support to Smallholder Tree Farmers

A decentralized seedling production is regarded as the most effective means of providing smallholders with planting materials. However, the sustainability of smallholder nursery operation is constrained by several factors, including the limited availability of germplasm, lack of technical skills and low seedling demand low viability of seedling market. The government nursery sector could complement the weaknesses of the smallholder nursery group but government nurseries are not operating effectively to enhance the availability of high quality seedlings of a wide species base for smallholder forestry. Seedling production in government nurseries is quantity-orientated with low emphasis on the production of high quality planting materials. Germplasm used in seedling production is mostly collected from unselected sources and seedlings produced are mostly of low physical quality. The species selection in government nurseries is heavily based on the availability of germplasm which resulted in the production of a few species which are commonly raised on private nurseries. The duplication of species of most seedlings raised in government nurseries with those produced in private nurseries and the far distance of government nurseries from the villages has resulted in low uptake of seedlings by smallholder tree farmers. For the government nursery sector to become more effective in providing support in the present paradigm of decentralized seeding production, a change from its production system and a broadening of the role it plays is needed. The social mobilization on seedling production as a scheme of implementing the Green Philippines Program has led the Department of Environment and Natural Resources to acknowledge the seedling production activity of private individual and communal nursery groups. This can be an opportunity for organizing the private and government nursery sectors to promote the operational effectiveness of the overall forest nursery industry.     

Comparative genetic analysis and molecular mapping of fertility restoration genes for WA,Dissi, and Gambiaca cytoplasmic male sterility systems in rice

The genetic relationship among three cytoplasmic male sterility (CMS) systems, consisting of WA, Dissi, and Gambiaca, was studied. The results showed that the maintainers of one CMS system can also maintain sterility in other cytoplasmic backgrounds. The F₁ plants derived from crosses involving A and R lines of the respective cytoplasm and their cross-combination with other CMS systems showed similar pollen and spikelet fertility values, indicating that similar biological processes govern fertility restoration in these three CMS systems. The results from an inheritance study showed that the pollen fertility restoration in all three CMS systems was governed by two independent and dominant genes with classical duplicate gene action. Three F₂ populations, generated from the crosses between the parents of good-performing rice hybrids, that possess WA, Dissi, and Gambiaca CMS cytoplasm, were used to map the Rf genes. For the WA-CMS system, Rf3 was located at a distance of 2.8 cM from RM490 on chromosome 1 and Rf4 was located at 1.6 cM from RM1108 on chromosome 10. For the Dissi-CMS system, Rf3 was located on chromosome 1 at 1.9 cM from RM7466 and Rf4 on chromosome 10 was located at 2.3 cM from RM6100. The effect of Rf3 on pollen fertility appeared to be stronger than the effect of Rf4. In the Gambiaca-CMS system, only one major locus was mapped on chromosome 1 at 2.1 cM from RM576. These studies have led to the development of marker-assisted selection (MAS) for selecting putative restorer lines, new approaches to alloplasmic line breeding, and the transfer of Rf genes into adapted cultivars through a backcrossing program in an active hybrid rice breeding program.     

Analysis of uniformity of sprinkle irrigation in a semi-arid area

One of the most appropriate sprinkler systems for arid or semi-arid areas – where a great deal of irrigating water is required – are the permanent set systems and the continuous-move laterals. To know the reality of water application in this type of areas, many field evaluations of solid set systems and centre pivot irrigation were conducted in Castilla-La Mancha region (Spain). The main factors affecting water application and evaporation and drift losses with these systems (pressure, wind speed, sprinkler type, etc.) were analysed. A set of performance guidelines and recommendations for the design and management of sprinkle irrigation is presented to attain the highest uniformity and efficiency in water application in semi-arid areas. To use working pressure as low as possible, but with sprinklers that produce a great deal of middle size water drops along with night irrigation for minimising evaporation and drift losses are important aspects.     

Usefulness of establishing trunk diameter based reference lines for irrigation scheduling in almond trees

The suitability of trunk diameter reference baselines for irrigation scheduling of mature drip-irrigated almond trees [Prunus dulcis (Mill). D.A. Webb cv Marta] was assessed in a 3-year experiment conducted on well-watered trees in south-eastern Spain. Continuous measurements of trunk daily growth rate (DGR) and maximum daily trunk shrinkage (MDS) were carried out throughout the growing season (April–November). Day-to-day variations in MDS and DGR were related with meteorological variables. DGR was found to be weakly correlated to all the meteorological variables, while MDS showed significant relationships with most of them. The mean air vapour pressure deficit during the period 10.00–15.00 h solar time (VPD_mx) was the environmental variable that best correlated with MDS. The relationships obtained were similar for all the phenological phases, except postharvest, when MDS showed a tendency towards lower values for a given atmospheric evaporative demand. The increase in trunk diameter over the 3 years also appeared to affect the relationships to some extent. As a practical procedure, it is proposed determining the baseline periodically (every 1 or 2 years) during the early stages (II–III) of the almond phenological cycle, for use as reference MDS for the subsequent growth stages, because over longer periods several factors affecting the stability of the baseline, in particular trunk diameter growth, might introduce changes in the baseline.     

Serum haemolytic and agglutinating activity as indicators of fish immunocompetence: their suitability in stress and dietary studies

Gilthead sea bream, Sparus aurata, were subjected to different stressful situations and the haemolytic activity mediated by the alternative complement and the agglutinating serum activity were tested as indicators of immunocompetence. The results show that both parameters were significantly compromised after chronic or repeated acute stress as well as in groups fed with vitamin E or 3 highly unsaturated fatty acids (HUFA) deficient diets and in infected fish. These results suggest that both haemolytic and agglutinating activity are suitable indicators for immunocompetence as they are non-specific responses induced by non-specific stressors. In addition, a comparison is performed with other immune indicators subjected to the same stressors.