Rural housing is related to plant invasions in forests of southern Wisconsin,USA

Forests throughout the US are invaded by non-native invasive plants. Rural housing may contribute to non-native plant invasions by introducing plants via landscaping, and by creating habitat conditions favorable for invaders. The objective of this paper was to test the hypothesis that rural housing is a significant factor explaining the distribution of invasive non-native plants in temperate forests of the Midwestern US. In the Baraboo Hills, Wisconsin, we sampled 105 plots in forest interiors. We recorded richness and abundance of the most common invasive non-native plants and measured rural housing, human-caused landscape fragmentation (e.g. roads and forest edges), forest structure and topography. We used regression analysis to identify the variables more related to the distribution of non-native invasive plants (best subset and hierarchical partitioning analyses for richness and abundance and logistic regression for presence/absence of individual species). Housing variables had the strongest association with richness of non-native invasive plants along with distance to forest edge and elevation, while the number of houses in a 1 km buffer around each plot was the variable most strongly associated with abundance of non-native invasive plants. Rhamnus cathartica and Lonicera spp. were most strongly associated with rural housing and fragmentation. Berberis thumbergii and Rosa multiflora were associated with gentle slopes and low elevation, while Alliaria petiolata was associated with higher cover of native vegetation and stands with no recent logging history. Housing development inside or adjacent to forests of high conservation value and the use of non-native invasive plants for landscaping should be discouraged.     

Smallholder reforestation and livelihoods in the humid tropics: a systematic mapping study

Systematic mapping studies provide a snapshot of the literature based on systematic literature searches. In this systematic mapping study, the original research that links reforestation and livelihoods in the tropics was mapped and analysed to identify the trends, biases and gaps in the literature. In total, 339 papers from 92 journals were identified. Agroforestry Systems was the journal in which articles were most frequently published, and Cameroon and Indonesia the most frequently studied countries. The greatest number of authors came from the USA, and authors were most commonly affiliated with ICRAF. A limited collaboration between research groups in the tropical regions was identified. Anthropology and Social Sciences were the most frequent areas of research, especially in Africa. Latin America had more technical studies and more publications discussing payment for environmental services than the other regions. Based on the temporal analysis of the main terms in abstracts of the publications included, it was found that agriculture-related terms and terms related to the human component in the landscape were consistently prevalent in the literature relating reforestation and livelihoods throughout time. Agroforestry systems were especially important in small-scale reforestation and livelihoods. Trends, biases and gaps were discussed. Broader cooperation between tropical regions and between clusters of authors would be beneficial for research and practice.     

MAGNETIC RESONANCE IMAGING OF THE NORMAL EQUINE BRAIN

The purpose of this investigation was to define the magnetic resonance (MR) imaging appearance of the brain and associated structures of the equine head. MR images were acquired in oblique dorsal (T2-weighted), sagittal (T1-weighted), and transverse planes (T2-weighted), using a magnet of 1.5 Tesla and a human body coil. Relevant anatomic structures were identified and labeled at each level. The resulting images provided excellent anatomic detail of the cranioencephalic structures. Annotated MR images from this study are intended as a reference for clinical imaging studies of the equine head, specially in the diagnosis of brain diseases in the horse.     

Comparative Histological Study of the Small Intestine in Lizards (Reptilia)

A light and electron microscopica study of the small intestine of four species of lizard was conducted. The organization of the small intestine is described and comparisons are made between the species examined.     

Glomerulonephritis and immunosuppression associated with dietary essential fatty acid deficiency in gilthead sea bream, Sparus aurata L., juveniles

An experiment was conducted to determine the effect of an essential fatty acid (EFA) deficient diet on growth, immune status and renal morphology of juvenile gilthead sea bream, using two diets: a control diet containing 2% (DW) of n-3 high unsaturated fatty acid (n-3 HUFA) and a diet formulated to be deficient in EFA for this species and containing 0.5% DW of n-3 HUFA (diet NFA). After 9 weeks of feeding the EFA-deficient diet fish showed a reduction in growth compared with the control group (107.48 +/- 9.14 and 123.14 +/-11.87 g final weight respectively). Fish fed the NFA diet showed a reduction in the erythrocyte volume together with increased erythrocyte fragility, haemoglobin content and red blood cell count in comparison with fish fed the control diet. EFA deficiency also reduced cellular immunity in terms of neutrophil activity and the number of circulating lymphocytes. The serum alternative complement pathway was markedly reduced in fish fed the EFA deficient diet. Fish fed the control diet showed glomeruli with a well-defined Bowman's space and normal renal tubes. However, up to 88% of fish fed the EFA-deficient diet showed alterations in renal morphology affecting at least 50% of the glomeruli, which showed extreme dilation of capillaries and occlusion of Bowsman's capsule. Mesangial proliferation and diffuse thickening of the capillary walls, as well as renal tube degeneration, were also observed.     

Identification of Atlantic bluefin tuna (Thunnus thynnus) stocks from putative nurseries using otolith chemistry

Chemical signatures in the otoliths of teleost fishes represent natural tags that may reflect differences in the chemical and physical characteristics of an individuals' environment. Otolith chemistry of Atlantic bluefin tuna (Thunnus thynnus) was quantified to assess the feasibility of using these natural tags to discriminate juveniles (age 0 and age 1) from putative nurseries. A suite of six elements (Li, Mg, Ca, Mn, Sr and Ba) was measured in whole otoliths using solution‐based inductively coupled plasma mass spectrometry. Otolith chemistry of age‐1 T. thynnus collected from the two primary nurseries in the Mediterranean Sea and western Atlantic Ocean differed significantly, with a cross‐validated classification accuracy of 85%. Spatial and temporal variation in otolith chemistry was evaluated for age‐0 T. thynnus collected from three nurseries within the Mediterranean Sea: Alboran Sea (Spain), Ligurian Sea (northern Italy), and Tyrrhenian Sea (southern Italy). Distinct differences in otolith chemistry were detected among Mediterranean nurseries and classification accuracies ranged from 62 to 80%. Interannual trends in otolith chemistry were observed between year classes of age‐0 T. thynnus in the Alboran Sea; however, no differences were detected between year classes in the Tyrrhenian Sea. Age‐0 and age‐1 T. thynnus collected from the same region (Ligurian Sea) were also compared and distinct differences in otolith chemistry were observed, indicating ontogenetic shifts in habitat or elemental discrimination. Findings suggest that otolith chemistry of juvenile T. thynnus from different nurseries are distinct and chemical signatures show some degree of temporal persistence, indicating the technique has considerable potential for use in future assessments of population connectivity and stock structure of T. thynnus.     

Evaluation of salt tolerance in rice genotypes by physiological characters

The use of physiological characters as selection criteria in salt tolerance breeding requires the identification of the contribution each individual character makes to salt tolerance. Rice genotypes were evaluated for salt tolerance in terms of grain yield and physiological characters. Plants of twelve genotypes were grown in sand tanks in a greenhouse and irrigated with Yoshida nutrient solution. Sodium chloride and calcium chloride (5:1 molar ratio) were added at two concentrations to give moderate (4.5 dS m^-1) and high (8.3 dS m^-1) salinity treatments. One set of plants was harvested at 635 ^°Cċd (accumulative thermal time) after planting to determine LAI and mineral ion concentrations. Another set of plants was allowed to grow to maturity. High genotypic diversity for LAI and shoot ion contents was observed. LAI contributed the most to the variation of the grain yield under salt stress. Significant correlations between LAI and yield components in both salt-tolerant and-sensitive genotypes further confirmed the significant contribution of LAI to grain yield. K-Na selectivity increased with increasing salinity. Conversely, Na-Ca selectivity decreased with increasing salinity. Significant correlations were identified between grain yield and both Na-Ca and K-Na selectivity. Highly significant (p<0.001) correlations were identified between Na-Ca selectivity and the rankings among genotypes for grain yield. Thus, Na-Ca selectivity could be one salt tolerance component and an useful selection criterion in screening for salt tolerance. This revised version was published online in July 2006 with corrections to the Cover Date.     

Importance of dietary arachidonic acid for the growth,survival and stress resistance of larval European sea bass (Dicentrarchus labrax) fed high dietary docosahexaenoic and eicosapentaenoic acids

Together with docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), arachidonic acid (ARA) is being considered to be an essential fatty acid in marine fish larval diets. The objective of the present study was to determine the importance of dietary ARA levels for larval European sea bass performance, when EPA and DHA are also present in the diet. Eighteen‐day‐old larvae were fed, for 14 days, gelatine‐based microdiets containing the following ARA levels: 0.3%, 0.6% or 1.2%. Elevation of dietary ARA up to 1.2% showed a positive correlation with larval survival and a significant improvement in the specific growth rates, body weight and total length. Arachidonic acid was efficiently incorporated into larval lipids, even at a higher proportion than that in the diets. Increased accumulation of ARA did not affect the incorporation of DHA or EPA from the diet into larval total lipids. A significant positive correlation was found between dietary ARA levels and survival after handling stress, indicating the importance of this fatty acid in sea bass larvae response to acute stressors. The results show the importance of ARA for sea bass larvae, but higher dietary levels should be tested to determine whether there is a negative effect of ARA in sea bass as reported for other species.     

Characterization of extracellular products from an isolate of Vibrio harveyi recovered from diseased post-larval Penaeus vannamei (Bonne)

Vibrio harveyi recovered from diseased post-larval Penaeus vannamei produced a thermostable exotoxin, which was lethal to Dublin Bay prawns, Nephrops norvegicus L., when injected intramuscularly. The extracellular products (ECPs) concentrated from tryptone soya broth supplemented with 1% (w/v) sodium chloride or from cellophane overlays on marine 2216E agar with incubation at 15, 22 and 27 °C were toxic, with the lethal dose 50% of the crude ECPs estimated to be 4.4 μgprotein prawn^−1. Proteolytic, haemolytic and cytotoxic activities were detected, although the occurrence and quantity of these activities were influenced by cultural conditions. The ECPs which had been heated (100 °C for 10 min) or digested with protease K produced the same pathology as crude, untreated ECPs. Western blotting demonstrated that all the ECP preparations contained low molecular weight lipopolysaccharides, which may constitute the lethal toxin of V. harveyi.