The effect of feeding behavior on Hg accumulation in the ecophysiologically different earthworms Lumbricus terrestris and Octolaseon cyaneum: A microcosm experiment

 A soil microcosm experiment was performed to assess the uptake of Hg from various Hg-spiked food sources (soil, leaf litter and root litter of Trifolium alexandrinum) by two earthworm species, Lumbricus terrestris (anecic) and Octolaseon cyaneum (endogeic). Treatments were applied in which one of the three food sources was Hg spiked and the other two were not. Additional treatments in which all or none of the food sources were Hg spiked were used as controls. Uptake of Hg from soil into tissues of both earthworm species was significantly higher than uptake of Hg from leaf litter or root litter, indicating that soil may be the most important pool for the uptake of Hg into earthworms. In addition, the anecic L. terrestris significantly accumulated Hg from all Hg-spiked food sources (leaf litter, root litter and soil), whereas the endogeic O. cyaneum took up Hg mainly from soil particles. Interestingly, there was no further increase in Hg in L. terrestris when all food sources were Hg spiked compared to the single Hg-spiked sources. This may be attributed to the relatively high Hg content in the soil, which may have influenced the feeding behavior of the earthworms, although their biomass did not significantly decline. We suggest that, in addition to the physiological differences, feeding behavior may also play a role in the contrasting uptake of Hg by the two earthworm species.     

Defective lipolysis and altered energy metabolism in mice lacking adipose triglyceride lipase

Fat tissue is the most important energy depot in vertebrates. The release of free fatty acids (FFAs) from stored fat requires the enzymatic activity of lipases. We showed that genetic inactivation of adipose triglyceride lipase (ATGL) in mice increases adipose mass and leads to triacylglycerol deposition in multiple tissues. ATGL-deficient mice accumulated large amounts of lipid in the heart, causing cardiac dysfunction and premature death. Defective cold adaptation indicated that the enzyme provides FFAs to fuel thermogenesis. The reduced availability of ATGL-derived FFAs leads to increased glucose use, increased glucose tolerance, and increased insulin sensitivity. These results indicate that ATGL is rate limiting in the catabolism of cellular fat depots and plays an important role in energy homeostasis.     

Long-term modelling of reservoir sedimentation with turbid underflows

Purpose

This paper presents the development of a turbid density current module (TDCM) for a one-dimensional quasi-steady reservoir sedimentation model suitable for long-term simulations and an example of its application.

Materials and methods

The module determines the plunging point of a density current based on the criterion of the densimetric Froude number. Following plunging, simulation continues in a supercritical regime as long as the energy of density flow and bed slope are sufficient to support transport in this mode, or until the dam is reached. A muddy pond is then formed. An adapted version of the theory of Toniolo et al. (2007) is used to compute water and sediment balance in a muddy pond. If the level of muddy pond exceeds the lowest level outlet, sediment is vented from the reservoir.

Results and discussion

The model was applied to one of the largest reservoirs in the world, the Nurek reservoir in Tajikistan. Historical data on bed levels and sediment inflow as well as a recent survey from 2015 were used to calibrate and validate the model. The model showed good results both in terms of modelled bed levels and composition of deposited sediment. For comparison purpose, the model was also calibrated without the TDCM module. However, the discrepancy between the observed and modelled data was significantly higher, confirming that the good match in the case of the TDCM module was not achieved solely by calibration, but rather by a more appropriate approach to modelling.

Conclusions

These results support the choice of using the model with TDCM for modelling a general pattern of long-term deposition in the Nurek reservoir, in which turbid density currents play an important role.

     

Effects on seedling development of direct contact of Pinus resinosa seeds or young seedlings with Captan1

Captan, at concentrations up to 2500 ppm did not affect seed germination of Pinus resinosa Ait. However, concentrations of 500 ppm or higher injured roots, stems, and cotyledons within 13 days. Root injury consisted of collapse of root hair cells, epidermal cells, and cortical cells; cotyledon injury of collapse of epidermal and mesophyll cells.     

The influence of plant litter diversity on decomposer abundance and diversity

Although there has been much recent interest in the effect of litter mixing on decomposition processes, much remains unknown about how litter mixing and diversity affects the abundance and diversity of decomposer organisms. We conducted a litter mixing experiment using litterbags in a New Zealand rainforest, in which treatments consisted of litter monocultures of each of 8 forest canopy and understory plant species, as well as mixtures of 2, 4 and 8 species. We found litter mixing to have little effect on net decomposition rates after either 279 or 658 days, and for each species decomposition rates in mixture treatments were the same as in monoculture. Litter species identity had important effects on litter microfauna, mesofauna and macrofauna, with different litter types promoting different subsets of the fauna. Litter mixing had few effects on densities of mesofauna and macrofauna, but did have some important effects on components of the microfauna, notably microbe-feeding and predatory nematodes. At day 279, litter mixing also consistently reduced the ratio of bacterial-feeding to microbe-feeding (bacterial-feeding+fungal-feeding) nematodes, pointing to mixing causing a significant switch from the bacterial-based to the fungal-based energy channel. Litter mixing sometimes influenced the community composition and diversity of nematodes and macrofauna, but effects of litter mixing on diversity were not necessarily positive, and were much weaker than effects of litter species identity on diversity. We conclude that litter mixing effects on the abundance and diversity of decomposer biota, when they occur, are likely to be of secondary and generally minor significance when compared to the effects of litter species identity and composition.