Effects of three esca-associated fungi on Vitis vinifera L.: III. Enzymes produced by the pathogens and their role in fungus-to-plant or in fungus-to-fungus interactions

When the esca-associated fungi Phaeomoniella chlamydospora (Pch), Togninia minima (Tmi) and Fomitiporia mediterranea (Fme) were grown in liquid stationary cultures, it was seen that they were able to live in media containing resveratrol (RES) or tannic acid (TA) as the sole carbon source and that the fungi were able to convert both compounds. Particular attention is paid here to detecting RES and TA conversion. Pch, Tmi and Fme were partially inhibited by RES or TA. Pch, Tmi and Fme produced extracellular tannase, laccase and peroxidase enzymes in liquid or agarized cultures, whether glucose was present or not. When colonies of Pch, Tmi and Fme were confronted, they showed spatially and temporally heterogeneous patterns of laccase and peroxidase activity. The results indicate the non-synergistic, competitive association of Pch and Tmi and the inhibition of Fme growth. Muconic acid, a well-known intermediate in a large number of lignin and phenol oxidative processes, can partly or completely inhibit the lignolytic agent Fme, but is tolerated by Pch and Tmi. An explanation for wood pigmentation patterns by Pch, Tmi and Fme is given.     

Nitrogen Release from Slow-Release Fertilizers in Soils with Different Microbial Activities

Soil microbial activity is recognized as an important factor affecting nitrogen (N) release from slow-release fertilizers. However,studies on the effect of size and activity of soil microflora on fertilizer degradation have provided contrasting results. To date, no clear relationships exist between soil microbial activity and the release of N from slow-release fertilizers. Hence, the aim of this study was to better understand such relationships by determining the release of N from three slow-release fertilizers in soils with different microbial activities. Soils were amended with urea-formaldehyde (UF), isobutylidene diurea (IBDU), and crotonylidene diurea (CDU). Urea, a soluble fertilizer, was used as the control. Fertilized soil samples were placed in a leaching system, and the release of N was determined by measuring ammonium-N and nitrate-N concentrations in leachates during 90 d of incubation. Non-linear regression was used to fit N leaching rate to a first-order model. In all the treated soils, N was released in the order: urea (89%–100%) IBDU (59%–94%) UF (46%–73%) CDU (44%–56%). At the end of incubation, N released from CDU did not differ (P 0.05) among soils. On the contrary, UF and IBDU released significantly lower (P 0.05) amounts of N in the soil with higher microbial activity and lower pH.The rate constant (K_0) for UF was lower (P 0.05) in the soil with lower pH. Taken together, our results indicated that soil microbial size and microbial activity had a marginal effect on fertilizer mineralization.     

Développements récents de l'Application de la chromatographie en phase gazeuse des aliments,et proposition d'Une nouvelle méthode complémentaire ou subsidiaire pour la micro-détermination spécifique De quelques composants, à l'aide de la diagnose enzymatique

Resumé La chromatographie en phase gazeuse étant à la fois une méthode qualitative et quantitative, constitue une méthode idéale destinée à résoudre la complexité des analyses des additifs alimentaires. Après une énumération des perfectionnements récents apportés à ces appareils, l'auteur fait un exposé de l'application et des résultats, notamment dans les domaines: Huile d'olive, Beurre, Saindoux, Vin, Cacao, Chocolat. Identification des acides gras, table des constituants, recherches de falsifications, analyse des substances étrangères, altération des vins, discussion des résultats d'analyse.Pour le dosage des résidus de pesticides et pour la recherche des agents antifermentaires, la chromatographie en phase gazeuse s'avère d'une grande utilité en raison de sa précision et sa rapidité.Comme un complément de la chromatographie en phase gazeuse l'auteur insiste sur l'importance, dans le secteur alimentaire, d'une nouvelle méthode d'analyse, basée sur des réactions enzymatiques et sur les études exécutées par son Institut pour la mise ou point de telles méthodes enzymatiques: détermination du glucose, du fructose, du saccharose dans les moûts et les vins, etc...

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Summary Gas-chromatography being a qualitative and quantitative method at the same time, makes an ideal method enabling us to solve the complexity of the analyses of food additives. After enumerating the recent improvements in the gas-chromatography equipment the author explains its applications and shows the results obtained, more specially in the following fields: olive oil, butter, lard, wine, cocoa, chocolate, identification of fatty acids, table of components, search for adulterants, analysis of foreign substances, deterioration of wines, discussion of the results of analyses.In the determination of pesticide residues, and the search for antiferments, gas-chromatography appears to be extremely useful, thanks to its precision and rapidity.As a supplement to gas-chromatography the author insists on the importance, in this field of foods, of a new analytical method based on enzymatic reactions, and on the investigations, carried on in this Institute, aimed to set up such enzymatic methods, to be used in the study of glucose, fructose, and saccharose deteriorations in musts and wines, etc...

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Zusammenfassung Die Gaschromatographie ist gleichzeitig eine quantitative und qualitative Analysenmethode. Sie ist daher hervorragend für die Lösung der komplexen Probleme auf dem Gebiete der Lebensmittel-Zusatzstoffe geeignet. Nach Aufzählung der letzten Verbesserungen in der Apparatetechnik, werden Anwendungsmöglichkeiten und Ergebnisse besprochen, insbesondere für Analysen von Olivenöl, Butter, Schmalz, Wein, Kakao und Schokolade. Bestimmung der Fettsäuren, Liste der Bestandteile, Nachweis von Fälschungen, Fremdstoffanalysen, Weinalterung, kritische Betrachtung der Ergebnisse.Für den Nachweis der Pestizid-Rückstände und die Bestimmung von Antifermenten, leistet die Gaschromatographie infolge der Genauigkeit und Schnelligkeit der Methode bereits große Dienste.Zur Ergänzung der gaschromatographischen Verfahren schlägt der Autor, insbesondere für Lebensmittelanalysen ein auf enzymatischen Reaktionen fußendes neues Verfahren vor. Hierzu sind die Untersuchungen in seinem Institut weit fortgeschritten, insbesondere für die Bestimmung von Glukose, Fruktose, Saccharose in Most und Wein.

     

Agriculture and society: Remarks on transformations and new social profiles in the case of Italy

In this paper the authors analyze the two most important interrelated processes of social change in Italian agriculture: first the increasing productive specialization of family farming, both full and part-time, lending to the persistence of small farms but also to their growing integration and complementarity with other economic activities; and second the increasing heterogeneity of agricultural workers accompanied by the destructuring of their strong working-class identity, which had matured in the previous decades. This identity, however, also reflected a deep separation between agricultural laborers, their labor market and institutions, and the rest of the working class. The authors argue that these changes are progressively contributing to the full integration of agricultural social relations within an increasingly complex society. The analysis of these two processes in recent Italian experience shows that this trend towards integration differs totally from the hypothesis often advanced in the past according to which the integration of agriculture would occur through the development of green factories, more and more similar to large Fordist manufacturing plants. On the contrary, at least in present-day Italy, integration means increasing heterogeneity, also of organizational patterns in manufacturing, and growing inter-dependence.     

Application of a low-cost camera on a UAV to estimate maize nitrogen-related variables

Precision Agriculture - The development of small unmanned aerial vehicles and advances in sensor technology have made consumer digital cameras suitable for the remote sensing of vegetation. In this...     

Daily rhythm of salivary and serum urea concentration in sheep

Background  

In domestic animals many biochemical and physiological processes exhibit daily rhythmicity. The aim of the present study was to investigate the rhythmic pattern of salivary and serum urea concentrations in sheep.     

Assay of aroma active components of virgin olive oils from southern Italian regions by SPME-GC/ion trap mass spectrometry

An SPME-GC/ion trap method was exploited to determine the chromatogram of volatile compounds of organic olive oils of southern Italian regions. The method is based on the assay of the terminal species of the "lipoxygenase pathway", which are present in the volatile fraction of the sampled compounds. Ethyl isobutanoate was used as internal standard in either the EI or CI ionization mode. The absolute concentration values of each analyte were evaluated through good-to-excellent calibration curves. Case studies on oils obtained from different cultivars or harvesting times are presented. The quantitative data for each compound were subjected to principal component analysis to characterize the different cultivars of this work.     

Multielement fingerprinting as a tool in origin authentication of PGI food products: Tropea red onion

Tropea red onion ( Allium cepa L. var. Tropea) is among the most highly appreciated Italian products. It is cultivated in specific areas of Calabria and, due to its characteristics, was recently awarded with the protected geographical indications (PGI) certification from the European Union. A reliable classification of onion samples in groups corresponding to "Tropea" and "non-Tropea" categories is now available to the producers. This important goal has been achieved through the evaluation of three supervised chemometric approaches. Onion samples with PGI brand (120) and onion samples not cultivated following the production regulations (80) were digested by a closed-vessel microwave oven system. ICP-MS equipped with a dynamic reaction cell was used to determine the concentrations of 25 elements (Al, Ba, Ca, Cd, Ce, Cr, Dy, Eu, Fe, Ga, Gd, Ho, La, Mg, Mn, Na, Nd, Ni, Pr, Rb, Sm, Sr, Tl, Y, and Zn). The multielement fingerprint was processed using linear discriminant analysis (LDA) (standard and stepwise), soft independent modeling of class analogy (SIMCA), and back-propagation artificial neural network (BP-ANN). The cross-validation procedure has shown good results in terms of the prediction ability for all of the chemometric models: standard LDA, 94.0%; stepwise LDA, 94.5%; SIMCA, 95.5%; and BP-ANN, 91.5%.     

Mixotrophy in a Local Strain of Nannochloropsis granulata for Renewable High-Value Biomass Production on the West Coast of Sweden

A local strain of Nannochloropsis granulata (Ng) has been reported as the most productive microalgal strain in terms of both biomass yield and lipid content when cultivated in photobioreactors that simulate the light and temperature conditions during the summer on the west coast of Sweden. To further increase the biomass and the biotechnological potential of this strain in these conditions, mixotrophic growth (i.e., the simultaneous use of photosynthesis and respiration) with glycerol as an external carbon source was investigated in this study and compared with phototrophic growth that made use of air enriched with 1–2% CO₂. The addition of either glycerol or CO₂-enriched air stimulated the growth of Ng and theproduction of high-value long-chain polyunsaturated fatty acids (EPA) as well as the carotenoid canthaxanthin. Bioassays in human prostate cell lines indicated the highest antitumoral activity for Ng extracts and fractions from mixotrophic conditions. Metabolomics detected betaine lipids specifically in the bioactive fractions, suggesting their involvement in the observed antitumoral effect. Genes related to autophagy were found to be upregulated by the most bioactive fraction, suggesting a possible therapeutic target against prostate cancer progression. Taken together, our results suggest that the local Ng strain can be cultivated mixotrophically in summer conditions on the west coast of Sweden for the production of high-value biomass containing antiproliferative compounds, carotenoids, and EPA.     

FEASIBILITY FOR USING HYPOFRACTIONATED STEREOTACTIC VOLUMETRIC MODULATED ARC RADIOTHERAPY (VMAT) WITH ADAPTIVE PLANNING FOR TREATMENT OF THYMOMA IN RABBITS: 15 CASES

Thymoma is a relatively common tumor in rabbits. Treatment with surgery, radiation therapy, and chemotherapy alone or in combination has been reported with varying outcomes. Stereotactic volumetric modulated arc radiotherapy delivered in a hypofractionated manner allows high doses of radiation to be delivered to the target volume while allowing sparing of adjacent critical structures. This therapy is ideally suited for thymomas in rabbits given their size, the difficulty of multiple anesthesia episodes and the complexity of the radiotherapy plans required due to the tumor's proximity to the heart, lungs, and mediastinal structures. Fifteen rabbits with thymoma were prospectively recruited for this observational, single institution, single arm clinical study. All rabbits were imaged with both computed tomography (CT) and magnetic resonance imaging (MRI). A total dose of 40 Gy in six fractions was delivered using a single arc over an 11‐day period with repeat CT simulation done every other fraction for adaptive planning. Follow‐up evaluation was done through repeat CT and MRI imaging and revealed complete responses using the Response Evaluation Criteria in Solid Tumors (RECIST) criteria. Two rabbits had died at 618 and 718 days, 10 were alive and three were lost to follow‐up. Observed acute and late effects were graded according to the Veterinary Radiation Therapy Oncology Group (VRTOG) criteria and were found to be minimal.