生化和ISSR分子标记在黑木耳品种鉴定中的应用

以生产收集的24个黑木耳栽培菌株为试材,采用拮抗、酯酶同工酶及ISSR分子标记技术对其进行分类鉴定和遗传多样性分析。结果表明:酯酶同工酶酶谱显示24个黑木耳菌株共扩增出11条迁移率不同的酶带,聚类分析表明当遗传系数为0.74时,将24个黑木耳菌株分为两大类;通过ISSR分子标记共扩增出67条清晰的DNA多态片段,大小介于0.2～2.0 kb,聚类分析表明当遗传系数为0.85时,将24个菌株分为两大类,聚类分析结果与拮抗试验结果基本一致。该研究为黑木耳的菌株选择及遗传育种提供了参考依据。     

青花菜新品种浙青80的选育

青花菜浙青80是利用萝卜质(ogura)雄性不育系技术选育的杂交品种,适合夏种秋冬收,从移栽至收获80 d左右。株型中等,较直立,株高约70 cm,开展度约70 cm;叶色深绿,侧枝少;花球紧实、颜色绿,遇低温不发紫,花蕾匀细,花球表面不易满天星,球茎不易空心,商品性好,球径15 cm左右,单球质量570 g左右。于2018年通过浙江省农作物品种认定委员会认定。     

Protective effect of curcumin analogue L6H4 on diaphragm of type 2 diabetic rats

AIM: To investigate the protective effect of curcumin analogue L6H4 on diaphragm of type 2 diabetic rats.METHODS: SPF male Sprague-Dawley rats (n=40) were randomly divided into 5 groups: normal control (NC) group, high fat (HF) group, high fat+L6H4 treatment (FT) group, diabetes mellitus (DM) group and DM+L6H4 treatment (DT) group. The rats in the later 4 groups were fed with high-fat diet. After 4 weeks of high-fat diet fee-ding, the rats in DM and DT groups were intraperitoneally injected with streptozotocin to induce type 2 diabetes melliutus. The rats in FT and DT groups were given L6H4 by gavage for 8 weeks. Blood glucose and blood lipid levels were detected biochemically. Fasting serum insulin (FINS) level was measured by radioimmunoassay and insulin resistance index (HOMA-IR) was calculated. Serum adiponectin (APN) level was measured by ELISA. The morphological changes of the diaphragm were observed under light and transmission electron microscopes. Lipid deposition and the activity of succinate dehydrogenase (SDH) and NADH-tetrazolium reductase (NADH-TR) were observed by enzyme histochemical staining. The content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in the diaphragm were measured by thiobarbituric acid method and hydroxylamine method, respectively. The protein expression of adiponectin receptor 1 (AdipoR1) in the diaphragm was determined by immunohistochemistry and Western blot. RESULTS: The levels of blood lipids, blood glucose, FINS and HOMA-IR in HF and DM groups were higher than those in NC group, but decreased after L6H4 treatment. The serum APN level in HF and DM groups was lower than that in NC group, but increased after treatment with L6H4. The muscle fibers of the diaphragm were shrunk, fat particles accumulated in the muscle fibers, and the mitochondria were slightly swollen in HF and DM groups. The diaphragmatic fibrosis was obvious in DM group. These lesions were relieved after L6H4 treatment. Compared with NC group, the level of MDA and the activity of SDH and NADH-TR in the diaphragm were increased in HF and DM groups, but decreased after treatment with L6H4. The activity of SOD and the expression of AdipoR1 in the diaphragm were lower than those in NC group, but increased after L6H4 treatment.CONCLUSION: The curcumin analogue L6H4 exerts a protective effect on diaphragm in type 2 diabetic rats. The strengthened protein expression of AdipoR1, the increased serum level of APN, and anti-lipid peroxidation may be involved in the process.     

气象因子对寒地水稻鞘腐病发生的影响

为明确主要气象因子对黑龙江省水稻主产区水稻鞘腐病发生的影响,从2007年开始连续10年进行定点调查,收集水稻鞘腐病发生时期的气象因子及病情指数,采用通径分析及逐步回归的方法,对影响水稻鞘腐病病情指数的气象因子进行了研究。结果表明,8月份的平均温度是对水稻鞘腐病影响最大的气象因子,其次为8月份的平均降雨量和9月份的平均日照时数。试验结果为适期施药防治水稻鞘腐病奠定了基础。     

农业司法鉴定收费问题的研究

分析了农业司法鉴定的特点、农业司法鉴定收费的现状与存在问题,提出了农业司法收费的性质、收费应考虑的因素、加快农业技术司法鉴定收费项目、标准建设和规范管理的对策建议。     

4种多年生禾草苗期抗旱性的比较研究

采用盆栽育苗法,在4种多年生禾本科牧草苗期连续干旱胁迫下测定植株的叶片相对含水量、质膜相对透 性、游离脯氨酸含量、丙二醛含量4个抗旱生理指标,以综合评价4种多年生禾草的抗旱性能。研究结果表明:供试材料 在连续干旱胁迫下,叶片相对含水量呈下降趋势,质膜相对透性、游离脯氨酸含量及丙二醛含量都有所增加。综合分析 结果,4种多年生禾草的苗期抗旱性按照从强到弱的顺序依次为无芒雀麦>苇状羊茅≥老芒麦>披碱草。     

阿维菌素·吡虫啉复配剂对菜蚜的室内毒力与田间防效

室内毒力测定结果显示,阿维菌素与吡虫啉等量复配对菜蚜(Lipaphiserysimi(Kaltenbach))的毒力指数分别为阿维菌素的1.6倍和吡虫啉的4.9倍,共毒系数达244.38,具有明显的增效作用。田间小区试验表明,使用1%阿·吡悬浮剂1000、2000、3000倍液防治菜蚜,药后7d的防效分别为98.79%、96.63%、94.31%。     

落叶松人工林根径材积表和合理经营密度研究

利用小兴安岭地区人工落叶松(Larix gmelinii)林标准地调查资料,建立了落叶松胸径d1.3与根径d0.0、胸径d1.3和冠径Cw的相关关系回归模型.结果表明,胸径d1.3与根径d0.0、胸径d1.3和冠径Cw的相关关系模型都以二次抛物线为最优;还利用胸径d1.3与根径d0.0的关系,编制了落叶松根径立木材积表,并探讨了落叶松人工林的理论密度和郁闭度为0.7和0.8时的适宜经营密度,对落叶松人工林的盗伐案件处理和经营管理具有一定的参考价值.     

分光光度法快速检测苏丹Ⅰ和Ⅳ

运用分光光度计建立快速有效的检测苏丹Ⅰ和Ⅳ的方法,优化苏丹红的萃取工艺.通过全波长分光光度计检测鸡蛋及饲料中苏丹I和Ⅳ的含量,结果显示用分光光度法检测苏丹Ⅰ和Ⅳ与HPLC法检测结果一致.     

大豆体细胞胚胎发生系统组织学研究

通过对栽培大豆品种体细胞胚胎发生频率组织学进行研究,了解在农杆菌和卡那霉素存在的条件下,大豆未成熟子叶胚性细胞的发生、分化、发育过程和胚胎发生规律,为大豆未成熟子叶诱导体细胞胚发生高效转化体系提供理论依据。