In vivo kinetic study on uptake and distribution of intramuscular tritium-labeled polysulfated glycosaminoglycan in equine body fluid compartments and articular cartilage in an osteochondrial defect model

The uptake and distribution of intramuscularly (IM) administered tritium-labeled polysulfated glycosaminoglycan (³H-PSGAG) in serum, synovial fluid, and articular cartilage of eight horses was quantitated, and hyaluronic acid (HA) concentration of the middle carpal joint was evaluated in a pharmacokinetic study. A full-thickness articular cartilage defect, created on the distal articular surface of the left radial carpal bone of each horse served as an osteochondral defect model. ³H-PSGAG (500 mg) was injected IM, between 14 and 35 days after creation of the defects. Scintillation analysis of serum and synovial fluid, collected from both middle carpal joints at specific predetermined times up to 96 hours post-injection, revealed mean ³H-PSGAG concentrations peaked at 2 hours post-injection. ³H-PSGAG was detected in cartilage and subchondral bone 96 hours post-injection in samples from all eight horses. There were no statistically significant differences in ³H-PSGAG concentration of synovial fluid or cartilage between cartilage defect and control (right middle carpal) joints.

HA assay of synovial fluid revealed concentrations significantly increased at 24, 48, and 96 hours post-injection in both joints. The concentration nearly doubled 48 hours post-injection. However, no statistically significant differences were found between synovial concentrations of HA in cartilage defect and control joints.

³H-PSGAG administered IM to horses, was distributed in the blood, synovial fluid, and articular cartilage. HA concentrations in synovial fluid increased after IM administration of polysulfated glycosaminoglycan.     

农机安全管理体制的变革和构想

结合《中华人民共和国道路交通安全法》有关规定和黑龙江省农机安全管理的实践,总结了农机安全管理体制焦点问题和体制的变革,并对实施道路交通安全法提出设想和建议。     

鸡肾型传染性支气管炎的诊断和防治要点

鸡的肾型传染性支气管炎(以下简称肾传支)多发生于1～5周龄的雏鸡群,在疫区流行时无明显的季节性,但相对低温期发病率更高。因该病主要通过呼吸道感染,在密集饲养的肉仔鸡群中易呈暴发性流行。该病自然病程约10d左右。一旦发病,除直接因原发病和继发病引起死亡外,还严重影响病愈鸡的生产性能,例如能损伤患病鸡的性腺发育,降低性成熟后的繁殖功能,使肉仔鸡脱水失重,发育滞后,甚至形成僵鸡。肾传支病程有以下进行规律,现介绍如下供诊断参考:急性期:以上呼吸道症状为主,两眼流泪,鼻有清涕,干咳甩头,有轻湿啰音。但病鸡应激敏感,能自由饮食,此时…     

Comments on the method to test side effects of pesticides on the predatory mitePhytoseiulus persimilis Ath.-Hen

A paper published byKniehase & Zoebelein (1990) describes a new laboratory method to test the side effects of pesticides on the predatory mitePhytoseiulus persimilis and criticizes the laboratory test developed by the International Working Group pesticides and beneficial organisms of the International Organization for Biological Control (IOBC), West Palaearctic Regional Section (WPRS). The present publication discusses the advantages and disadvantages of both methods, responds to the criticism and mention the overall concept of the IOBC/WPRS Working Group including semifield and field test methods.In einer Arbeit vonKniehase undZoebelein (1990) wird ein neues Laborverfahren zur Prüfung der Nebenwirkung von Pflanzenschutzmitteln auf die RaubmilbePhytoseiulus persimilis beschrieben. Die genannten Autoren äußern sich kritisch über das seither von der Arbeitsgruppe Pflanzenschutzmittel und Nutzorganismen der International Organization for Biological Control (IOBC), West Palaearctic Regional Section (WPRS) praktizierte Verfahren. In der vorliegenden Arbeit werden die Vor- und Nachteile der beiden Prüfmethoden diskutiert. Einer Reaktion auf die Kritik folgt eine kurze Beschreibung der gesamten Konzeption der genannten Arbeitsgruppe, die auf einer Kombination aus Labor-, Halbfreiland- und Feldprüfverfahren beruht.     

海南油棕果腐病的病原鉴定及发病条件的研究

三年来作者对于幼龄結果油棕的果穗和果实腐烂的症状、分布、蔓延为害,其发生条件和栽培管理的关系进行了詳細的調查和观察,并进行了病原分离、培养、田间人工接种和試探性化学保护等試驗。同时对果实离层組織的形成进行了切片检查。 結果指出海南十二个地区的幼龄结实棕园普遍出現的花、果、穗腐与环境条件和栽培管理有密切关系。果腐是果实离体后从蒂部組織开始的。从腐果組織中經常可以分离到細菌、炭疽菌和鐮刀菌。多次田間接种証明这些菌对健康果实和果穗均无致病能力。大田喷药无效。看来,油棕果腐病是由于环境坏、管理差的条件下,未成熟或接近成熟的果实产生离层而与果柄分离,再由外界杂菌腐食脫果而致腐烂。可見,本病是屬于非侵染性的生理病害。     

防治地老虎杀卵兼治初龄幼虫的试验

两年来室内和試驗場試驗証实:有机磷杀虫剂如1605及敌百虫等能兼治小地老虎和黄地老虎的卵和初龄幼虫;有机氯杀虫剂如DDT及666等对卵几乎无效,但对初龄幼虫效果頁好。两类药剂混用杀卵效果提高。大田試驗用50％敌百虫乳剂1份、25％DDT乳剂1份、水600份,在卵孵化率为28.6—95.0％时亩施混合液50公斤,防治效果良好;防治适期比药杀初龄幼虫可提早十天左右,大大緩和了时間紧张的矛盾。     

推动江苏现代种业强省建设的路径和政策研究

根据江苏加快建设农业强省要求,种业作为农业全产业的源头,种业强省建设势在必行。在分析种业基础信息和调查研究的基础上,梳理当前江苏种业发展现状和主要特点,分析了当前江苏种业发展存在的弱项与短板,提出建设“当家品种强、市场主体强、竞争能力强、支撑保障强”的现代化种业强省江苏路径,并提出相关政策建议。