白菜类富含亮氨酸重复（LRR）抗病蛋白基因BcLRR cDNA克隆及其介导的植物软腐病抗性分析

利用前期构建的软腐欧文氏菌(Erwinia carotovora subsp.carotovora)菌株BC1侵染诱导表达的大白莱(Brassica campestris subsp.pekinensis)SSH cDNA文库和cDNA芯片.在芯片分析的基础上,选取一个与拟南芥(Arabidopsis thaliana)类富含亮氨酸重复(LRR)抗病蛋白Cf-5高度同源的EST序列(GenBank登录号:DN962361)进行进一步分析.用5'-和3'-RACE方法克隆了该基因全长1 286 bp的cDNA序列,包含一个完整ORF和3'UTR,并具有Poly(A)加尾信号,将其命名为BcLRR基因(GenBank登录号:EU424347).BcLRR推导蛋白序列由327个氨基酸组成,除N端跨膜螺旋结构域外,大部分为LRR结构域,包含8个胞外LRR重复单元,由7个规范的LXXLXLXN结构和1个不规范LXXLXXXN组成.氨基酸序列比对分析发现,BeLRR蛋白与拟南芥类LRR抗病蛋白Cf-5同源性高达96.6%,与棉花(Gossypium hirsutum L.)类LRR抗病蛋白GhLRR同源性为82.3%,它们之间大部分变异限定在N端信号肽区.系统发育关系表明,在三种植物中功能均未知的这一蛋白属于一个新的胞外LRR蛋白类型,在结构上不同于其它抗性已知的胞外型LRR蛋白.将受CaMV 35S启动子驱动的BcLRR基因完整ORF序列转化拟南芥Col-0,PCR鉴定、Southern杂交和RT-PCR分析表明,BcLRR基因已整合到拟南芥基因组中,并在拟南芥中获得了转录.软腐病病情指数分析表明,转BcLRR植株提高了对软腐欧文氏菌的抗性.     

浅论《孙子兵法》谋略思想对大学古代汉语教学的启示

《孙子兵法》这部我国古代最早最伟大的兵书,作为一种能激发学生兴趣的教学资源,它的理论在选择教师、教师做好授课前的准备、教学中采用灵活多样的授课方法等方面给古汉语教学提供了有益的启示。     

地方文化资源数字化保护与文化创意品牌塑造

以惠州龙门农民画产业化为例,惠州龙门农民画是以"南蛮文化"作为独特视角,展现南国传统民俗风情的民间绘画,被誉为世界民间艺术奇葩。分析了产业化发展进程中龙门农民画的艺术特色和文化价值,阐述了基于知识管理的龙门农民画数字化保护思路,并从文化创意角度对龙门农民画的文化品牌塑造进行了探讨。     

ENDOSCOPIC ULTRASOUND INSTRUMENTATION, APPLICATIONS IN HUMANS, AND POTENTIAL VETERINARY APPLICATIONS

Endoluminal scanning under endoscopic guidance, or endoscopic ultrasonography (EUS), has become the most significant advance for imaging the gastrointestinal (GI) tract wall and contiguous organs in the past 20 years. It was originally designed to overcome the limitations in humans to imaging the abdominal organs transabdominally, such as large penetration depths and GI air. This imaging modality provides detailed images of pathological processes both within and outside of the GI wall since a high-frequency transducer can be brought into close proximity with the target regions. It has found most success in humans for the staging of lung, gastric, and esophageal cancer, the detection of both lymphatic and hepatic metastases, and diagnosis of pancreatitis and pancreatic cancer, as well as achieving an important role in interventional and therapeutic procedures. The EUS examination can be performed to examine both the thorax and abdomen in animals when both conventional transthoracic or transabdominal ultrasound are inadequate due to intervening air, bone, large penetration depths, or obesity. The echoendoscope is similar to a conventional endoscope but has an ultrasound transducer at its tip. Both radial and linear multifrequency scanners are available. Linear scanners allow fine-needle aspiration (FNA) of the bowel wall or extraluminal structures. Transducer coupling is either by direct mucosal contact or by inflation of a water-filled balloon surrounding the transducer. Current thoracic applications for EUS in veterinary medicine include examination of the mediastinum, bronchial lymph nodes, esophagus, and pulmonary lesions as well as FNA of pulmonary masses. Abdominal applications include examination of both pancreatic limbs and the liver, including portosystemic shunts, detection of lymphadenomegaly, and examination of the gastric wall, duodenum, and jejunum. Other potential applications in dogs and cats include tumor staging and intrapelvic ultrasound.     

6-BA和GA3促进三色堇再生的研究

为了解决三色堇生产周期长，观赏期短等问题，对三色堇进行了再生利用的研究。结果表明，对过期三色堇进行刈割，留茬再生，喷施6－BA和GA3，并加强肥水管理，能生产出具有良好商业价值的再生三色堇。其中喷施6－BA的花朵数多、花大、叶宽、株型适中，具有良好的观赏价值，观赏期延长1倍。     

DOPPLER MEASUREMENT OF SPLANCHNIC BLOOD FLOW DURING DIGESTION IN UNSEDATED NORMAL DOGS

The purpose of this study was to measure splanchnic blood flow during digestion in unsedated dogs by using duplex Doppler sonography. The study population consisted of 12 healthy dogs. Blood flow in the cranial mesenteric artery, the celiac artery, and the aorta was measured before a test meal and at 20, 60, and 90 minutes after eating. The following measurements were made or calculated: vessel diameter, peak systolic velocity, end diastolic velocity, mean velocity, resistive index, pulsatility index, and flow volume. There was a significant postprandial decrease in the resistive and pulsatility indices in both the cranial mesenteric (preprandial RI = 0.867, postprandial RI = 0.796, preprandial PI = 3.033, postprandial PI = 2.173) and the celiac (preprandial RI = 0.854, postprandial RI = 0.769, preprandial PI = 2.639, postprandial PI = 1.930) arteries. In both vessels the end diastolic velocity, the mean velocity, and the flow volume increased significantly postprandially. These changes occurred significantly earlier in the celiac artery than in the cranial mesenteric artery. The findings most likely correspond to postprandial splanchnic vasodilation. Doppler ultrasound provide a good methode of detecting changes in postprandial splanchnic blood flow in the dog.     

Research on PDM Technology Oriented Dynamical Data

对冬枣采后主要病害种类、症状及防治方法进行阐述,并介绍了生物防治技术在果蔬贮运病害防治中的应用,展望了该技术在冬枣采后病害防治中的应用前景.     

浙江省西部地区双低油菜品比试验

选用多个双低油菜品种在浙江省西部山区进行试种，筛选出适合本生态区种植的优质、高产双低油菜品种。试验结果表明：杂2005-1生育期适中，产量高，可参加下一年度省级区试。湘杂油3号、沪油杂1号，丰产性好，可分别进一步试验示范和参加下一年度区试。