New Perspectives in the Chemistry of Marine Pyridoacridine Alkaloids

Secondary metabolites from marine organisms are a rich source of novel leads for drug development. Among these natural products, polycyclic aromatic alkaloids of the pyridoacridine type have attracted the highest attention as lead compounds for the development of novel anti-cancer and anti-infective drugs. Numerous sophisticated total syntheses of pyridoacridine alkaloids have been worked out, and many of them have also been extended to the synthesis of libraries of analogues of the alkaloids. This review summarizes the progress in the chemistry of pyridoacridine alkaloids that was made in the last one-and-a-half decades.     

The crop as indicator for sidedress nitrogen demand in sugar beet production — limitations and perspectives

In 2‐years field experiments near Nienstädt (60 km west of Hannover, northern Germany), the effects of rate and timing of nitrogen (N) application on leaf N‐concentration, leaf greenness (SPAD chlorophyll meter readings), canopy greenness (canopy light reflectance), leaf area development, photosynthetic activity of leaves, and yield and quality of sugar beet were studied. In 1999 (pre‐planting soil mineral N: 15 kg ha^—1), N fertilizer was applied at rates of 0, 105, 125, 145, 165 and 205 kg N ha^—1. In 2000 (pre‐planting soil mineral N: 60 kg ha^—1), an N rate of 100 kg ha^—1 was applied at planting (100/0/0/0) or split applied at planting and 8 (60/40/0/0), 12 (60/0/40/0), and 16 (60/0/0/40) weeks after planting (WAP), respectively. In both years, canopy greenness as indicated by ”︁sensor values” (a combination of the reflectance of visible and near infrared light) changed with crop age. However, at each time of measurement, sensor values precisely reflected the different N application treatments and were significantly correlated with leaf N‐concentrations and SPAD chlorophyll meter readings. Beet yield and processed white sugar yield increased up to an N supply (fertilizer N + pre‐planting soil mineral N) of 160 kg ha^—1. Split N application slightly retarded leaf growth but had no effect on photosynthetic activity per unit leaf area. Beet yield and beet quality were not systematically affected by the timing of N application. Certain application schemes tended to favor either beet yield or beet quality, resulting in similar processed white sugar yields. Our data suggest that moderate N topdressing can be integrated in site‐specific N management systems in sugar beet production. Canopy light reflectance might serve as a useful diagnostic tool to assess the N status and sidedress N demand of sugar beets. However, due to changing sensor values over time, on‐site calibration (using established standard methods or reference plots receiving extra N at planting) will be necessary. The applicability of this approach has to be tested in further field studies.<?show $6#>     

Mobilization of phosphorus contributes to positive rotational effects of leguminous cover crops on maize grown on soils from northern Nigeria

Previous research has demonstrated a positive rotational effect of tropical leguminous cover crops on maize growth on a luvisol from Nigeria. This effect could not be explained by a better N supply. The objective of the present work was to further clarify whether improved P nutrition has been a contributing factor. Nine cover crops and maize were studied in nutrient solution‐culture with 1 and 20 μM P and with NO₃‐N as N source for root physiological parameters that may affect P mobilization. Zea mays, Lablab purpureus, and Centrosema pubescens responded to P deficiency by higher rates of proton excretion. Clitoria ternatea excreted OH^— with only small differences due to P nutrition. At low P supply, Chamaecrista rotundifolia, Clitoria, and Centrosema had the highest exudation rates of organic acid anions, especially citrate and malate. A major difference between plant species was found in root‐surface acid phosphatase activity. Cajanus cajan expressed the highest phosphatase activity. V_max of P uptake increased markedly under P deficiency, particularly for maize. Compared to the other plant species Cajanus, Chamaecrista, and Clitoria were characterized by a greater capability to absorb P at low external P concentrations. The nine cover crops and maize were also grown in pot experiments using two soils from northern Nigeria low in available P. The ultisol from Jos had a high P fixation capacity and was more acidic than the alfisol from Zaria. All plant species were precultured in the first season at 100 (Zaria) and 250 (Jos) mg P per pot. In the subsequent season, maize was uniformly seeded into all pots containing the remaining roots and the incorporated shoot dry mass (according to 15 mg P per pot) of the pre‐crop. No P and 250 (Zaria) or 500 (Jos) mg P per pot were freshly applied. Maize growth and P uptake were enhanced after legumes in Zaria soil. Cajanus showed the highest residual effect, and also Clitoria, Chamaecrista, and Lablab showed effects superior to the mean. In Jos soil, Clitoria, Cajanus, and Lablab enhanced maize growth above average. Also, a highly significant positive correlation between P uptake and biomass production was obtained. These results indicate that enhancement of maize growth after leguminous cover crops, at the low P supply of the soil used, mainly depends on P mobilization capacity of the cover crop.     

Determination of the fate of C labelled maize and wheat rhizodeposit-C in two agricultural soils in a greenhouse experiment under C-CO2-enriched atmosphere

A deeper understanding of the contribution of carbon (C) released by plant roots (rhizodeposition) to soil organic matter (SOM) can help to increase our knowledge of global C-cycling. These insights can eventually lead to sustainable management of SOM especially in agricultural systems. This study was conducted to determine the fate of ¹³C labelled rhizodeposit-C of maize and wheat plants. They were grown in a greenhouse in permeable nylon bags filled with upper soil material from two agricultural soils of the same location, but with different crop yields. The bags were placed into pots, which were also filled with soil surrounding the bags. Soil inside the bags was considered as rhizosphere soil, wheras the one outside the bags represented bulk soil. The contributions of rhizodeposits to water extractable organic carbon (WEOC), microbial biomass-C (MB-C), CO₂-C evolution, and total organic carbon (C_org) were investigated during a 7-week growing period. The WEOC, MB-C, CO₂-C, C_org contents and the respective δ¹³C values were determined regularly, and a newly developed method for determining δ¹³C values in soil extracts was applied.In both soils, regardless of crop yield potential, significant incorporation of rhizodeposition-derived C was observed in the MB-C, CO₂-C, and C_org pool, but not in the WEOC. The pattern of C incorporation into the different pools was the same for both soils with both plants, and rhizodeposit-derived C was recovered in the order MB-C<C_org<CO₂-C. This showed that rhizodeposits were mainly respired, but since C_org was the second largest pool of the overall balances, they were also stabilized in the soils at least in the short term. It is suggested that the increased SOM mineralization observed in this study (positive priming effects) was probably induced by C exchange processes between the soil matrix and soluble rhizodeposits. Moreover, soluble rhizodeposit-C was detected in MB-C and CO₂-C evolved outside the direct root zone, showing the availability of these C-components in the bulk soil.