全文获取类型
收费全文 | 673篇 |
免费 | 32篇 |
国内免费 | 3篇 |
专业分类
林业 | 61篇 |
农学 | 35篇 |
基础科学 | 4篇 |
121篇 | |
综合类 | 109篇 |
农作物 | 29篇 |
水产渔业 | 47篇 |
畜牧兽医 | 237篇 |
园艺 | 8篇 |
植物保护 | 57篇 |
出版年
2023年 | 7篇 |
2022年 | 5篇 |
2021年 | 15篇 |
2020年 | 8篇 |
2019年 | 16篇 |
2018年 | 17篇 |
2017年 | 26篇 |
2016年 | 24篇 |
2015年 | 12篇 |
2014年 | 12篇 |
2013年 | 25篇 |
2012年 | 46篇 |
2011年 | 43篇 |
2010年 | 27篇 |
2009年 | 28篇 |
2008年 | 50篇 |
2007年 | 42篇 |
2006年 | 22篇 |
2005年 | 26篇 |
2004年 | 26篇 |
2003年 | 31篇 |
2002年 | 24篇 |
2001年 | 8篇 |
2000年 | 6篇 |
1999年 | 5篇 |
1998年 | 5篇 |
1997年 | 3篇 |
1995年 | 6篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1991年 | 7篇 |
1990年 | 4篇 |
1989年 | 10篇 |
1988年 | 4篇 |
1986年 | 8篇 |
1985年 | 5篇 |
1984年 | 5篇 |
1983年 | 5篇 |
1982年 | 6篇 |
1979年 | 5篇 |
1978年 | 3篇 |
1977年 | 6篇 |
1976年 | 4篇 |
1975年 | 6篇 |
1972年 | 3篇 |
1971年 | 3篇 |
1969年 | 6篇 |
1967年 | 4篇 |
1964年 | 3篇 |
1939年 | 2篇 |
排序方式: 共有708条查询结果,搜索用时 0 毫秒
21.
Intan Mariana Maliki Mailin Misson Peik Lin Teoh Kenneth Francis Rodrigues Wilson Thau Lym Yong 《Marine drugs》2022,20(2)
Marine algae are an excellent source of novel lectins. The isolation of lectins from marine algae expands the diversity in structure and carbohydrate specificities of lectins isolated from other sources. Marine algal lectins have been reported to have antiviral, antitumor, and antibacterial activity. Lectins are typically isolated from marine algae by grinding the algal tissue with liquid nitrogen and extracting with buffer and alcohol. While this method produces higher yields, it may not be sustainable for large-scale production, because a large amount of biomass is required to produce a minute amount of compound, and a significant amount of waste is generated during the extraction process. Therefore, non-destructive extraction using algal culture water could be used to ensure a continuous supply of lectins without exclusively disrupting the marine algae. This review discusses the traditional and recent advancements in algal lectin extraction methods over the last decade, as well as the steps required for large-scale production. The challenges and prospects of various extraction methods (destructive and non-destructive) are also discussed. 相似文献
22.
Enterotoxigenic Escherichia coli (ETEC)-associated post-weaning diarrhea (PWD) is economically one of the most important diseases for the swine industry. Porcine ETEC strains typically express K88 or F18 fimbria and heat-labile (LT) and/or heat-stable (STa, STb) enterotoxins. However, recent studies indicate that EAST1 toxin, adhesin involved in diffuse adherence (AIDA-I) and porcine attaching and effacing-associated factor (paa) may also be expressed by ETEC strains associated with diarrhea. To better understand the virulence factors of E. coli strains that cause PWD, we applied PCR to screen for K88, F18, F41, 987P and K99 fimbrial genes; LT, STa, STb, Stx2e and EAST1 toxic genes; and AIDA-I, paa and EAE adhesin genes in E. coli strains recently isolated from young pigs with PWD in the US. Of 304 E. coli isolates from diarrheic pigs submitted for testing, 175 (57.6%) strains possessed fimbrial genes: K88 (64.6%), F18 (34.3%), F41 (0.57%), K99 (0.57%), 987P (0); toxin genes: LT (57.7%), STb (72.6%), STa (27.4%), STx2e (17.4%), EAST1 (35%); and adhesin genes: AIDA-I (26.9%), paa (60%), EAE (1.1%). All toxin genes except the EAST1 toxin gene, were almost exclusively associated with K88+ or F18+ isolates, and most of these isolates carried multiple toxin genes. The non-fimbrial adhesin paa was found present in over half of the K88+ isolates. A total of 129 (42%) isolates carried no fimbrial genes, including 66 (21.7%) isolates that did not have any of the above virulence genes. These results suggest a broad array of virulence genes associated with PWD in pigs. 相似文献
23.
Aleman M Magdesian KG Peterson TS Galey FD 《Journal of the American Veterinary Medical Association》2007,230(12):1822-1826
CASE DESCRIPTION: A 4-month-old American Paint filly was evaluated because of sudden onset of ataxia that progressed to recumbency. Five additional horses from the same and neighboring premises developed signs of poor performance, generalized weakness, ataxia, and recumbency; 2 of those horses were also evaluated. A new batch of a commercial feed supplement had been introduced to the horses' diet on each farm within the preceding 3 days. CLINICAL FINDINGS: Other than recumbency, findings of physical and neurologic examinations of the foal were unremarkable. The other 2 horses had generalized weakness and mild ataxia, and 1 horse also had persistent tachycardia. The foal had mild leukocytosis with neutrophilia, hyperglycemia, and mildly high serum creatine kinase activity. Results of cervical radiography, CSF analysis, and assessments of heavy metals and selenium concentrations in blood and vitamin E concentration in serum were within reference limits. Feed analysis revealed high concentrations of the ionophore antimicrobial salinomycin. TREATMENT AND OUTCOME: The 5 affected horses survived, but the foal was euthanized. At necropsy, a major histopathologic finding was severe vacuolation within neurons of the dorsal root ganglia, which was compatible with ionophore toxicosis. The surviving horses developed muscle atrophy, persistent weakness, and ataxia. CLINICAL RELEVANCE: In horses, ionophore toxicosis should be considered as a differential diagnosis for acute weakness, ataxia, recumbency, or sudden death. Furthermore, ionophore toxicosis should be considered as a cause of poor performance, weakness, muscle wasting, and cardiac arrhythmias in horses. Surviving horses may have impaired athletic performance. 相似文献
24.
25.
26.
Ron A. Miller Francis R. Pelsor Andrew S. Kane Renate Reimschuessel 《Journal of aquatic animal health》2013,25(2):121-128
Abstract The pharmacokinetic–pharmacodynamic predictor of antimicrobial activity for tetracyclines is reported to be the area under the concentration–time curve at steady state (AUCss) divided by the minimal inhibitory concentration of the targeted pathogen. Here, we estimate AUCss values for oxytetracycline (OTC) in serum of rainbow trout Oncorhynchus mykiss by using a destructive sampling study design. Seventy-two rainbow trout were fed OTC-medicated feed at 74.7 ± 1.5 mg/kg (mean ± SD) body weight (BW) by oral gavage for 10 consecutive days. Serum was collected from nine fish at 1, 3, 6, 8, 10, 12, 15, and 22 d after dosing began. Serum OTC concentrations were measured by high-performance liquid chromatography with a 0.01-μg/mL limit of detection. The average OTC AUCss was 29.2 μg × h/mL and was estimated using nonlinear mixed-effects modeling and bootstrap resampling techniques. The elimination half-life was estimated as 85.0 h, and the fraction of steady state achieved was estimated as 0.85. The calculated AUCss (24.8 μg × h/mL) following 10 d of oral dosing with 75 mg OTC/kg BW was less than the estimated AUCss. Results suggest that the pharmacokinetics of OTC exposure, including the AUCss, is better evaluated by using multiday dosimetry than by using a standard single-dose protocol. Received September 29, 2011; accepted January 30, 2012 相似文献
27.
Gabriele Vaccari Cynthia H. Panagiotidis Cristina Acin Simone Peletto Francis Barillet Pierluigi Acutis Alex Bossers Jan Langeveld Lucien van Keulen Theodoros Sklaviadis Juan J. Badiola Olivier Andr��oletti Martin H. Groschup Umberto Agrimi James Foster Wilfred Goldmann 《Veterinary research》2009,40(5)
Scrapie is a fatal, neurodegenerative disease of sheep and goats. It is also the earliest known member in the family of diseases classified as transmissible spongiform encephalopathies (TSE) or prion diseases, which includes Creutzfeldt-Jakob disease in humans, bovine spongiform encephalopathy (BSE), and chronic wasting disease in cervids. The recent revelation of naturally occurring BSE in a goat has brought the issue of TSE in goats to the attention of the public. In contrast to scrapie, BSE presents a proven risk to humans. The risk of goat BSE, however, is difficult to evaluate, as our knowledge of TSE in goats is limited. Natural caprine scrapie has been discovered throughout Europe, with reported cases generally being greatest in countries with the highest goat populations. As with sheep scrapie, susceptibility and incubation period duration of goat scrapie are most likely controlled by the prion protein (PrP) gene (PRNP). Like the PRNP of sheep, the caprine PRNP shows significantly greater variability than that of cattle and humans. Although PRNP variability in goats differs from that observed in sheep, the two species share several identical alleles. Moreover, while the ARR allele associated with enhancing resistance in sheep is not present in the goat PRNP, there is evidence for the existence of other PrP variants related to resistance. This review presents the current knowledge of the epidemiology of caprine scrapie within the major European goat populations, and compiles the current data on genetic variability of PRNP. 相似文献
28.
This article outlines the principal gastrointestinal protozoal diseases that have been recorded as affecting reptiles. It covers 9 genera of flagellates/amoebae, 1 ciliate genus, and 5 genera of coccidia, describing their pertinent anatomy and what is known about their epidemiology, including clinical presenting signs and intestinal pathological changes. The agents are initially discussed individually and, to avoid repetition, common information about diagnostics, treatment, and control is then presented. 相似文献
29.
Identification of walnut husk (Juglans regia L.) volatiles and the behavioural response of the invasive Walnut Husk Fly,Rhagoletis completa Cresson 下载免费PDF全文
30.