Isolation of differentially expressed genes during interactions between tomato cells and a protective or a non-protective strain of Fusarium oxysporum

Pathogenic isolates of Fusarium oxysporum applied on a non-host plant species, as soil-borne non-pathogenic isolates, are able to protect this plant against pathogenic strains inducing wilts. Several modes of action contribute to the biocontrol activity of these protective strains; however the genetic basis of the biocontrol mechanisms is far from being understood. The aim of this study was to identify genes involved in biocontrol activity of F. oxysporum using an original model made of Fom24, a strain protective on tomato and its mutant rev157 which has lost its protective capacity. A Rapid Subtractive Hybridization (RaSH) approach was chosen to identify genes up-regulated in the protective or in the non-protective interaction when germinated conidia of either Fom24 or rev157 are confronted to tomato cell cultures. A total of 86 up-regulated sequences were generated, 42 and 44 from the protective and the non-protective interaction respectively. Homology searches led to identification of both plant and fungal genes that were grouped according to their putative functions. Among plant genes, those involved in plant response to stresses were the most abundant. Expression profiles of genes homolog to a basic endochitinase, a ferredoxine-NADP(H) reductase (FNR), an ATP synthase and the RPM1-interacting protein 4 (RIN4) were confirmed by Northern blotting. A large proportion of fungal sequences were encoding genes of unknown function; among other, those involved in response to oxidative stress and a gene putatively encoding an enolase are the most promising to further study their potential role in the protective interaction between F. oxysporum and tomato.     

EFFECT OF DIFFERENT ROOTSTOCKS ON GROWTH,CHLOROPHYLL A FLUORESCENCE AND MINERAL COMPOSITION OF TWO GRAFTED SCIONS OF TOMATO

Two tomato scions (cvs. ‘Raf’ and ‘Gorety’) were grafted on three different rootstocks: S. torvum, ‘Beaufort’ (Lycopersicum esculentum × Lycopersicum hirsutum) and intermediate grafting of eggplant ‘Cristal’ between tomato and S. torvum (double graft). Plants were grown in Mediterranean greenhouse conditions. The response to grafting was measured through growth parameters, F_v/F_m and leaf macronutrients analysis, and it was compared with non-grafted plants. The scions grafted on S. torvum in simple and double graft showed lower fresh and dry weight of leaves, number of commercial fruits, plant height, F_v/F_m and decreased their capacity to absorb several nutrients resulting in a lower mineral concentration in scions leaves, as a result of a thickened graft union. On the other hand, both scions showed a good response when grafted on the rootstock ‘Beaufort’, with which growth parameters, yield and photosynthetic capacity were similar to non-grafted plants.     

Postharvest regulated deficit irrigation in ‘Summit’ sweet cherry: fruit yield and quality in the following season

We examined, over the postharvest seasons of 2005–2007, regulated deficit irrigation (RDI) for its potential of saving water and maintaining fruit yield and quality in ‘Summit’ sweet cherry. The postharvest irrigation treatments were: full irrigation (Control), receiving 80% of water in Control (RDI-80%), and receiving 50% of water in Control (RDI-50%). Midday stem water potential (Ψ_stem) was used for assessing plant water status. In 2006, trees produced a large crop and commercial fruit thinning had to be applied, whereas 2007 was a low crop year. The RDI treatment, first applied in 2005, reduced fruit set in 2006 and also reduced root winter starch concentration. In 2006, fruit set was lower in RDI-50% than in Control. But fruit thinning had still to be done with the final yield being the same among treatments. In 2007, RDI-50% produced more fruit and higher yields than Control. Relationship between postharvest Ψ_stem and crop load in the following season varied according to the year. They were negatively correlated in 2006 and positively correlated in 2007. Fruit firmness did not vary with irrigation treatments in any of the years. Fruit soluble solid concentration (SSC) and fruit relative dry matter (RDM) for RDI-50% was the highest in 2006 when RDI-50% trees had the lowest fruit set. In 2007, SSC and RDM for RDI-50% were the lowest with the trees having the highest fruit set and crop load at harvest. This study indicates that RDI-50% firstly applied in an “off” year, after crop has been harvested, can maintain fruit yield at similar levels to fully irrigated trees while saving water by 45%. Correction of biennial bearing and partial saving of thinning costs are additional advantages of this treatment.     

A conceptual framework for the governance of multiple ecosystem services in agricultural landscapes

Landscape Ecology - While the concept of ecosystem services (ES) is well established in the scientific and policy arenas, its operationalization faces many challenges. Indeed, ES supply, demand and...     

Phenology and drought affects the relationship between daily trunk shrinkage and midday stem water potential of peach trees

Summary

The relationship between maximum daily shrinkage in trunks (MDS), daily trunk growth (DTG), predawn water potential (Ψ_pd) and midday stem water potential (Ψ_stem) were studied in an irrigation experiment in peach trees. Control trees were irrigated to replace evapotranspiration, with trees receiving regulated deficit irrigation (RDI) watered at 35% of this rate during Stage II of fruit development and after harvest. The RDI trees were watered as controls during Stage III of fruit development. Minimum (Ψ_pd and Ψ_stem fell to –0.6.MPa and –1.2 MPa, respectively in RDI plots compared with –0.2 and –0.6 MPa in the controls. Trunk growth was less in the RDI plots than in the controls during drought. In contrast, MDS was higher when deficit irrigation was applied in the RDI trees. When site differences were considered the correlation between (Ψ_pd and accumulated trunk growth over an ample period was loose, while maximum daily shrinkage and midday stem water potential remarkably improved such a correlation. However, pooling all available data, the correlation between Ψ_stem and MDS was very poor (R²=0.44) and it substantially improved only when using data from specific phenological periods (i.e. R²=0.75). A seasonal drift in MDS values was observed and it was related to the seasonal changes in trunk growth rates, (i.e. highest shrinkage was found when growth rates were lowest). We concluded that phenology in combination with drought reduce the reliability of the water status information obtained from MDS.     

Donor effect on cultured pearl nacre development and shell matrix gene expression in Pinctada margaritifera reared in different field sites

Understanding the respective roles played by donor and recipient pearl oysters in pearl quality determination in relation to the environment is a challenge for the pearl industry. In most Pinctada species, pearl size is mainly related to recipient oyster growth performance but also relies to some extent on the biomineralisation activity of the pearl sac, a tissue that originates from the donor oyster mantle. We examined donor effect on pearl size in response to culture in the lagoons on Arutua and Apataki atolls. Overall, nacre weight and thickness were greater in Arutua than in Apataki, but sensitivity to the environment differed between donors. Some donors were associated with significantly heavier and thicker nacre in Arutua (I group), while others had similar results at the two sites (NI group). On average, up to 20% of the pearl size could be attributed to the donor but, in group I, donor effect was responsible for up to 36% of nacre weight determination. Additionally, a real‐time PCR expression study of eight matrix protein genes related to biomineralisation in the pearl sac showed that MSI60, pearlin and pif177 were significantly and positively correlated with nacre weight and thickness, with the latter two genes explaining the larger pearl size observed in Arutua. Donor oysters in P. margaritifera therefore play a key role in pearl size improvement, related to the role of the shell matrix protein genes. Understanding such contributions could help in the design of genetic selection plans for specific and adapted donor oyster lines.     

Tracking Agrobacterium strains by a RAPD system to identify single colonies from plant tumours

A molecular typing system for Agrobacterium strains based on the polymerase chain reaction–random amplified polymorphic DNA (PCR–RAPD) procedure was developed. It employs one to four different 10-mer primers and the results are highly reproducible. The band patterns obtained with the four primers for each of the 39 Agrobacterium strains analysed were different enough to differentiate the strains from each other. Strains with similar chromosomal background but different plasmid content, e.g. strains C58 and A281, gave the same band pattern with all the primers. Ten host plants were inoculated with eight Agrobacterium strains and the isolates obtained from the resulting tumours were analysed using the RAPD system developed here. The procedure allowed rapid identification of isolates recovered from tumours by comparison of their band patterns with band patterns of strains used as inoculum. The procedure also discriminated the various strains analysed. Purified bacterial cell suspensions, used for RAPD analyses, produced the same results as purified DNA, and greatly simplified the procedure. This system can be applied for rapid screening of Agrobacterium-like colonies isolated from plant tumours for epidemiological and genetic diversity studies.     

Growth patterns and morphology of fine roots of size-controlling and invigorating peach rootstocks

We compared growth patterns and morphology of fine roots of size-controlling and invigorating peach (Prunus persica (L.) Batsch) rootstocks. Peach trees were grafted on five rootstocks: a vigorous control (Nemaguard), three intermediate vigor rootstocks (K119-50, P30-135 and Hiawatha), and a semi-dwarfing rootstock (K146-43). Minirhizotron tubes were installed at the base of trees on each rootstock and root images captured with a minirhizotron digital camera system. Number, visible length, and diameter of new roots were recorded at fixed soil depths from April 19, 2000 to December 19, 2001. Root diameter, specific root length, root tissue density and root length density were also measured periodically for each rootstock on roots collected from in-growth cores. Rootstocks had similar seasonal patterns of new root production. Fine root production was lowest in winter and appeared to decline during the final stages of fruit growth. A rootstock with almond in its genetic background (K119-50) produced the greatest quantity of fine roots and had the greatest number of new roots below 69 cm, whereas there were no differences among the other four rootstocks in the total number of roots produced. Rootstock K146-43 had thicker fine roots than the other rootstocks. Independent of rootstock, fine roots produced during spring had greater specific root length than those produced later in the season. The seasonal pattern of fine root production did not appear to be associated with the previously reported effects of these dwarfing rootstocks on shoot growth and stem water potential early in the growing season.     

Seasonal sensitivity of stem water potential to vapour pressure deficit in grapevine

Boundary lines of stem water potential (Ψ_stem) responses to vapour pressure deficit (VPD) have been reported for several species and are generally referred to as VPD reference lines (RL). In order to study the response of Ψ_stem to VPD, RLs were determined in plants that received full (Control) and deficit (SSDI) irrigation during three consecutive years. The Control plants received irrigation equivalent to full crop water evapotranspiration minus effective rainfall and the SSDI plants were irrigated at 50% of the Control level. Ψ_stem values for the Control treatment during crop development tended to decrease, and those corresponding to SSDI plants were always lower than those for Control plants. Considering the 3-year data set, no relationship was found between Ψ_stem and VPD. However, there was a differential seasonal response between Ψ_stem and VPD, which depended on the stage of fruit development. A separate phenological analysis enabled the detection of RL for stage II (from fruit-set to veraison) and for stage III (post-veraison), whereas during stage I (from bud-break to fruit set) RL was not apparent. RL slopes increased as the season progressed and were significantly correlated to average values of Ψ_stem. The seasonal decrease in midday Ψ_stem for Control plants was interpreted as being a result of a progressive increase in canopy size and water consumption, which led to increased water depletion before each afternoon’s daily irrigation event. The apparent lack of RL during stage I was related to lower levels of water demand and high Ψ_stem.     

Influence of branch autonomy on fruit,scaffold, trunk and root growth during stage III of peach fruit development

We studied the influence of branch autonomy on the growth of reproductive and vegetative organs by establishing different patterns of fruit distribution within and between large branch units (scaffolds) in mature peach trees (Prunus persica (L.) Batsch cv. 'Elegant Lady'). Different patterns of fruit distribution were established by defruiting either whole scaffolds (uneven fruit distribution between scaffolds; US) or several selected hangers (small fruiting branches) per tree (uneven fruit distribution between hangers; UH). The effects of these patterns were compared with the effects of an even fruit distribution treatment (EVEN) in which fruits were thinned to achieve maximum uniformity of fruit distribution within the canopy. The desired fruit loads were obtained by differentially thinning the remaining bearing parts. On a tree basis, the response of mean fruit mass to fruit load was strongly affected by fruit distribution. The steepest mean fruit mass to fruit load relationship was found in US trees, whereas the relationship in UH trees was intermediate between the US and EVEN trees. On a scaffold basis, differences in fruit size between EVEN and US trees with similar fruit loads, though statistically significant, were relatively small, indicating that scaffolds were almost totally autonomous with respect to dry matter partitioning to fruit during the final stage of peach fruit growth. Hangers also appeared to exhibit significant autonomy with respect to the distribution of dry matter during the final phase of fruit growth. Branch autonomy was evident in scaffold growth: defruited scaffolds in the US treatment grew more than fruited scaffolds, and fruit distribution treatments had little impact on scaffold cross-sectional area on a tree basis. On the other hand, as observed for fruit growth, branch autonomy did not appear to be complete because the fruited scaffolds grew more in US trees than in EVEN trees under heavy cropping conditions. However, the effect of fruit distribution occurred only over short distances, and was negligible on organs located farther away from the source of heterogeneity (fruits), such as the trunk and roots.