Molecular structures of fructans from Agave tequilana Weber var. azul

Agave plants utilize crassulacean acid metabolism (CAM) for CO(2) fixation. Fructans are the principal photosynthetic products generated by agave plants. These carbohydrates are fructose-bound polymers frequently with a single glucose moiety. Agave tequilana Weber var. azul is an economically important CAM species not only because it is the sole plant allowed for tequila production but because it is a potential source of prebiotics. Because of the large amounts of carbohydrates in A. tequilana, in this study the molecular structures of its fructans were determined by fructan derivatization for linkage analysis coupled with gas chromatography-mass spectrometry (GC-MS), nuclear magnetic resonance (NMR), and matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-MS). Fructans were extracted from 8-year-old A. tequilana plants. The linkage types present in fructans from A. tequilana were determined by permethylation followed by reductive cleavage, acetylation, and finally GC-MS analysis. Analysis of the degree of polymerization (DP) estimated by (1)H NMR integration and (13)C NMR and confirmed by MALDI-TOF-MS showed a wide DP ranging from 3 to 29 units. All of the analyses performed demonstrated that fructans from A. tequilana consist of a complex mixture of fructooligosaccharides containing principally beta(2 --> 1) linkages, but also beta(2 --> 6) and branch moieties were observed. Finally, it can be stated that fructans from A. tequilana Weber var. azul are not an inulin type as previously thought.     

Surface-associated proteins of wheat starch granules: suitability of wheat starch for celiac patients

Wheat starch is used to make baked products for celiac patients in several European countries but is avoided in the United States because of uncertainty about the amounts of associated grain storage (gluten) proteins. People with celiac disease (CD) must avoid wheat, rye, and barley proteins and products that contain them. These proteins are capable of initiating damage to the absorptive lining of the small intestine in CD patients, apparently as a consequence of undesirable interactions with the innate and adaptive immune systems. In this study, starch surface-associated proteins were extracted from four commercial wheat starches, fractionated by high-performance liquid chromatography and gel electrophoresis, and identified by tandem mass spectrometry analysis. More than 150 proteins were identified, many of which (for example, histones, purothionins, and glutenins) had not been recognized previously as starch-associated. The commercial starches were analyzed by the R-5 enzyme-linked immunosorbent assay method to estimate the amount of harmful gluten protein present. One of these starches had a low gluten content of 7 ppm and actually fell within the range proposed as a new Codex Alimentarius Standard for naturally gluten-free foods (maximum 20 ppm). This low level of gluten indicates that the starch should be especially suitable for use by celiac patients, although wheat starches with levels up to 100 ppm are deemed safe in the proposed Codex standards.     

Residue depletion of nitrofuran drugs and their tissue-bound metabolites in channel catfish (Ictalurus punctatus) after oral dosing

The depletion of the nitrofuran drugs furazolidone, nitrofurazone, furaltadone, and nitrofurantoin and their tissue-bound metabolites [3-amino-2-oxazolidinone (AOZ), semicarbazide (SC), 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), and 1-aminohydantoin (AH), respectively] were examined in the muscle of channel catfish following oral dosing (1 mg/kg body weight). Parent drugs were measurable in muscle within 2 h. Peak levels were found at 4 h for furazolidone (30.4 ng/g) and at 12 h for nitrofurazone, furaltadone, and nitrofurantoin (104, 35.2, and 9.8 ng/g respectively). Parent drugs were rapidly eliminated from muscle, and tissue concentrations fell below the limit of detection (1 ng/g) at 96 h. Peak levels of tissue-bound AMOZ and AOZ (46.8 and 33.7 ng/g respectively) were measured at 12 h, and of SC and AH (31.1 and 9.1 ng/g, respectively) at 24 h. Tissue-bound metabolites were measurable for up to 56 days postdose. These results support the use of tissue-bound metabolites as target analytes for monitoring nitrofuran drugs in channel catfish.     

Analysis of host genotype x rhizobial strain interaction in N2 fixation

Summary A method of analyzing host genotype x rhizobial strain interaction for N₂ fixation potential, based on the principle of structural relationship analysis, is proposed. When this interaction is significant, selecting genotypes for high N₂ fixation potential is incorrect, because the N₂ fixation efficiency is dependent on the rhizobial strain used. Under such circumstances, grouping genotypes based on average fixing ability (AFA), a linear response to the effectiveness of rhizobial strain, and specific fixing ability (SFA), deviation from the linear response in terms of the magnitude of the error variance, is useful before initiating a breeding program for enhanced N₂ fixation. Host genotype x strain interaction effect is partitioned into two components, and two parameters are derived which estimate AFA and SFA. N₂ fixation data from 3 mungbean genotypes and 6 rhizobial strains are used to illustrate this method.     

A study of the effect of the interaction between site-specific conditions, residue cover and weed control on water storage during fallow

In the semiarid central region of Argentina the probability that rainfall meets crop requirements during growing season is less than 10%, therefore fallowing has been the most important practice to assure water availability during the growing season. Various site-specific and management factors have been identified as crucial for defining fallow efficiency (FE) and final available water contents (AW). The objective of the present study was to improve our knowledge about the interactions between residue cover, weed control, soil profile depth and water storage capacity (WSC) on FE. In 10 sites covering the environments of calcareous plains and sandy plains of the semiarid central region of Argentina and with different WSC, experiments with 3 different levels of residue cover (H, M, L) and with and without weed control (C and W respectively) during fallow were set up. A completely randomized block design with four repetitions and splits plots to consider weed control was used. Soil texture and organic matter were determined in samples of the A horizon (0.20 m). Bulk density, field capacity, permanent wilting point and soil water contents (monthly frequency) were measured at depth intervals of 0.20 m to the depth of the calcite layer or to 2.00 m depth. Soil temperature was taken in weekly intervals at 0.05 m depth and weed plants, separated by species, were counted at the end of fallow in 4 repetitions of 0.25 m² in each treatment. An empirical model was developed to predict final AW under these experimental conditions. Model parameters were: Residue level, weed control, WSC, profile depth, and rainfall during fallow. Site-specific conditions (WSC and profile depth) affected water storage during fallow; soils with highest values for both parameters showed highest final AW. Weed density was the most important factor that controlled AW, with on average 35 mm less AW in W than in C treatments. Residue level had a positive effect on final AW in both C and W treatments, with a difference of 18.5 mm between H and L. An interaction between residue level and weed density was observed, indicating weed suppression in H treatments. This was also confirmed by correspondence analysis between residue level and weed species which revealed that different species were related to each level. High residue levels also decreased soil temperature, thus affecting germination of post-fallow crops. The empirical model had an overall average prediction error of 13.7% and the regression between measured and predicted values showed a determination coefficient of 0.77.     

A comparative study of apple and pear tree water consumption measured with two weighing lysimeters

A five-year experiment (2002–2006) was conducted to determine apple (cv ‘Golden Smoothee’) and pear (cv ‘Conference’) crop coefficients (Kc) using two large weighing lysimeters. Daily reference evapotranspiration (ETo) and crop evapotranspiration (ETc) were evaluated. Midday canopy light interception of both crops planted in hedgerows, 4 × 1.6 m, was determined on a weekly basis from bud-break until leaf fall from year 2002 (fourth after planting) to year 2006 of both plantations. Relationships between canopy light interception and calculated Kc (ETc/ETo) were evaluated from bud-break until harvest. There were differences in Kc values between apple and pear trees. When daily Kc values from bud-break until harvest were adjusted to hyperbolic functions each year, adjusted curves for pear trees were very similar regardless of year (maximum Kc around 1.0). In apple trees, the maximum values of Kc increased over time, from 0.49 in 2002 to 1.04 in 2006. Midday light interception in both apple and pear trees increased during the 5 years of experiment from 29.0 to 45.6% in apples and from 27.5 to 41.6% in pears in midsummer. Although there was a significant positive correlation between midday canopy light interception and Kc in apple and pear trees, in different times within a specific year, these relationships were different between crops. While the apple data fitted into the same equation regardless of the year, different equations were needed to fit the pear data in different years. This discrepancy may have been related to differences in the canopy properties between apple and pear trees. Pear canopies had higher porosity than apple canopies and thus improved light penetration. Apple trees were more vigorous and produced taller and denser canopies. Pear Kc values were greatly influenced by the evaporative demands of different years and consequently differences in midday canopy light interception did not adequately reflect the differences in Kc across the two species.     

Competitive adsorption of water soluble plasma proteins from egg yolk at the oil/water interface

Water soluble plasma proteins were fractionated from hen's egg yolk, and the molecular weight and pI of the most abundant protein species were characterized with gel electrophoresis. The proteins were identified by mass spectrometry. The protein fraction was used to produce oil-in-water emulsions, both at various protein concentrations and at various pH values, and the surface load was determined through serum depletion. The competitive adsorption was studied through the determination of nonadsorbing species with gel electrophoresis. The results show that it was possible to form an oil-in-water emulsion for which droplet size and maximum surface load depended on the protein concentration and pH. Serum albumin and YGP40 adsorbed selectively at the oil/water interface throughout the pH range investigated, and for albumin the selectivity increased close to its pI. It is suggested that this selective adsorption is due to long hydrophobic stretches in the polypeptide chain, which are present in the selectively adsorbing species but absent in less adsorbing species.     

Influence of organic amendments on Fe,Cu, Mn,and Zn availability and clay minerals of different soils

The effects of application of composted olive mill wastewater sludge (A) and depotassified sugarbeet vinasse (V) on total diethylenetriaminepentaacetic acid (DTPA) and sequential extracted micronutrients were investigated. The mineralogy of the fine fraction of soils was also studied. The soils used were a Typic Rhodoxeralf (soil R), a Typic Xeropsamment (soil S), and a Typic Xerorthent (soil C). Fertilization with A and V during 3 years, in general did not significantly affect the total concentration of Fe, Cu, Mn, and Zn versus the control. However, the elements extracted with DTPA generally increased with the organic amendments, more with A than with V. The BCR (European Community Bureau of Reference) sequential extraction indicated that the addition of organic matter generally increased Zn and Mn in the two more available fractions. A great amount of Fe was found in the second and third fractions from the unamended and amended soils. Nevertheless, the sum of the three fractions was enhanced for the organic amendment, except for calcareous soil. The distribution of these elements in the different fractions was significantly affected by the type of soil. The addition of both fertilizers caused modifications in particle size and consequence redistribution of the calcite content between the different fractions.     

Removal of carbaryl, linuron, and permethrin by Lupinus angustifolius under hydroponic conditions

The metabolism of organic pollutants by plants normally requires contaminant direct uptake by cells. Factors affecting this uptake and the later distribution of chemicals within the plant include the physicochemical properties of the compounds (concentration, structure, solubility, log k(ow), diffusion rate) and the biochemical characteristics of the plant. This paper reports the tolerance, uptake, and effects of the pesticides carbaryl, linuron, and permethrin on Lupinus angustifolius germination and growth as well as contaminant intraplant distribution and possible degradation. Lupine plants were grown in hydroponic culture containing either 1 or 5 mg of the individual pesticides, or combinations of these (1, 5, or 10 mg of each), in 100 mL nutrient and water solutions. Analysis of the remaining solutions 8 days post-germination showed the water solutions to have higher remaining pesticide concentrations than nutrient solutions. Furthermore, in the presence of pesticides, germination was more frequent in the water solutions. After 16 days of growth, the plants were harvested, and their tissues were microwaved digested and analyzed by reversed-phase liquid chromatography. Although only minor quantities of each pesticide were detected in plant tissues, their amount in the roots was higher than in the stems. No accumulation was noted in the cotyledons, and only 2% of linuron was detected in the leaves. Mass recovery at the end of the experiment showed that 57, 53, and 55% of carbaryl, linuron, and permethrin, respectively, were degraded and/or bound in an irreversible manner to plant material. The results suggest that L. angustifolius could be useful for the cleaning/remediation of pesticide-contaminated water.