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101.
102.
Mariana Garcia Kako Rodriguez Giovanna Serpa Maciel Ricardo Andres Ramirez Uscategui Victor Jos Correia Santos Ricardo Perecin Nociti Priscila Del Aguila da Silva Marcus Antnio Rossi Feliciano Felipe Zandonadi Brando Jeferson Ferreira Fonseca Maria Emilia Franco Oliveira 《Reproduction in domestic animals》2019,54(3):456-463
The aim was to compare the early luteal development in ewes superovulated with different doses of pFSH. Twenty‐nine Santa Inês ewes received a progesterone device (CIDR®) for 8 days. Gonadotrophic treatment started on Day 6: G200 (control, n = 9, 200 mg); G133 (n = 10, 133 mg); and G100 (n = 10, 100 mg of pFSH). On Day 6, all females received eCG (300 IU). B‐mode and spectral Doppler ultrasonography were performed daily during the early luteal phase (Days 11–15) to monitor the development of corpora lutea (CLs; dimensions) and ovarian arteries indices. CLs were also classified as normal or prematurely regressed (PRCL) on Day 15 by videolaparoscopy. Ewes from G100 and G133 showed gradual increase in luteal diameter during the early luteal phase (p < 0.001), whereas G200 animals presented increase from Day 11 to Day 13, and then decrease on Days 14 and 15 (p < 0.001). The G200 females showed greater percentage of PRCL (45.20%) than those of the other groups (p < 0.001). The normal CLs number was greater in G100 than in G133 (p = 0.04), while the PRCL number was greater in G200 than in the other groups (p = 0.03). Resistive index (RI) was greater in G200 than in G100 (p = 0.02). RI was lower in Day 12 than Day 15 (p = 0.02). Pulsatility index (PI) was greater on Days 14 and 15 (p < 0.01). In conclusion, the lowest dose of pFSH (100 mg) can be considered sufficient for an efficient superovulatory response in sheep, producing better CLs development dynamic in early luteal phase and ovarian blood perfusion and smaller number of PRCL than the traditional (200 mg) pFSH dose. 相似文献
103.
Jeferson Ferreira da Fonseca Fabiana Nunes Zambrini Jos Domingos Guimares Marcio Roberto Silva Maria Emilia Franco Oliveira Felipe Zandonadi Brando Pawel Mieczyslaw Bartlewski Joanna Maria Gonalves Souza‐Fabjan 《Reproduction in domestic animals》2019,54(1):118-125
This study examined the feasibility of transcervical embryo recovery after the hormonal treatment to induce cervical dilation, following the 7‐day oestrous synchronization protocol in multiparous Santa Inês ewes. A total of 23 cyclic ewes received two doses of 37.5 μg of d‐cloprostenol by latero‐vulvar route 7 days apart. After the second injection of d‐cloprostenol, the ewes were checked for oestrus (every 12 hr) and then mated by fertile rams throughout the oestrous period. All ewes received 37.5 μg of d‐cloprostenol (latero‐vulvar) and 1 mg of oestradiol benzoate by either intramuscular (EBim group; n = 12) or intravaginal (EBivg group; n = 11) route 16 hr before embryo flushing. Twenty minutes before the flushing, 50 IU of oxytocin were administered intravenously. The oestrous response (i.e., the percentage of ewes that showed signs of oestrous behaviour after the second d‐cloprostenol injection) was 91.3% (21/23). The proportion of successfully penetrated ewes (81.8% compared with 80.0%), the mean duration of embryo flushing (24.7 ± 2.0 min compared 26.2 ± 1.9 min), the flushing fluid recovery rate (94.8 ± 1.3% compared with 91.0 ± 2.9%) and the average number of structures recovered per ewe (0.5 ± 0.4 compared with 0.8 ± 0.4) did not vary (p > 0.05) between the EBim and EBivg groups. Viable embryos were recovered from 41.2% (7/17) of successfully penetrated ewes. It can be concluded that nonsurgical (i.e., transcervical) embryo collection can be performed in oestrous‐synchronized Santa Inês ewes pretreated with d‐cloprostenol, oxytocin and oestradiol benzoate, with the latter hormone administered by either the intramuscular or intravaginal route. 相似文献
104.
Juliana Dantas Rodrigues Santos Eduardo Kenji Nunes Arashiro Mrio Felipe Alvarez Balaro Joanna Maria Gonalves Souza‐Fabjan Pedro Henrique Nicolau Pinto Clara Vieira de Souza Ceci Ribeiro Leite Jeferson Ferreira da Fonseca Felipe Zandonadi Brando 《Reproduction in domestic animals》2019,54(1):126-128
This study evaluated the cervical transposition method as a tool to select ewes for embryo collection by transcervical route. Adult Santa Inês ewes (n = 50) received Day 0 protocol for superovulation treatments. The cervix transposition test was performed both at oestrus and at the embryo collection time. The latter was preceded by hormonal cervical dilation. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy were 85.7%, 66.6%, 85.7%, 66.6% and 80.0%, respectively. The kappa index yielded a moderate score (κ = 0.52). In conclusion, the high sensitivity and accuracy indicate that the cervical transposition test is a screening option to select ewes for embryo collection by transcervical route. 相似文献
105.
Daniel Mageste de Almeida Marcos Inácio Marcondes Luciana Navajas Rennó Lívia Vieira de Barros Carla Heloísa Avelino Cabral Leandro Soares Martins David Esteban Contreras Marquez Felipe Vélez Saldarriaga Faider Alberto Castaño Villadiego Manuela Acevedo Cardozo Roman Maza Ortega Javier Enrique Garces Cardenas Virginia Lucia Neves Brandão Mário Fonseca Paulino 《Tropical animal health and production》2018,50(8):1771-1777
Beef cows’ milk yield is typically determined by measuring milk yield once daily and then doubling this value to estimate daily production. However, it is not known whether this is accurate. Thus, we aimed to determine the association between morning and afternoon milk yield in grazing Nellore cows. Eighty Nellore cows were used, with initial weight of 516.0?±?1.0 kg. The experiment was a completely randomized factorial scheme, with 20 replications and four treatments (i.e., +?or ??pre-partum supplementation in combination with +?or ??post-partum supplementation): PRMM—1 kg of supplement/cow/day for 90 days pre-partum; MMPS—1 kg of supplement/cow/day for 90 days post-partum; PRPS—1 kg of supplement/cow/day for 90 days pre-partum and 90 days post-partum; and MM—only mineral mix ad libitum during pre- and post-partum. Milk was sampled on days 45, 135, and 225 post-partum (early, middle, and late lactation, respectively). No effects were observed of pre- and post-partum supplementation on milk yield (P?>?0.05). The afternoon/morning proportion of 0.45 in the early third of lactation was higher than other stages, which had a proportion of 0.41 (P?<?0.05). Post-partum supplementation increased milk protein in the morning and afternoon milking (P?<?0.05). There was also no effect of pre- and post-partum supplementation on afternoon-morning proportion other milk components (P?>?0.05). We conclude that estimating daily milk production of grazing beef cattle by multiplying a once daily milking amount times two is not accurate. Under the conditions of this study, proportion of total daily production represented by the ratio of afternoon/morning milking was 0.45 in early lactation (first third) and 0.41 in mid- and late lactation. 相似文献
106.
107.
Mayara F. Brito Patrícia A. Auler Guilherme C. Tavares Cristiana P. Rezende Gabriel M. F. Almeida Felipe L. Pereira Carlos A. G. Leal Arlindo de Alencar Moura Henrique C. P. Figueiredo Marc Henry 《Reproduction in domestic animals》2018,53(5):1243-1246
The study aimed to describe the Bubalus bubalis seminal plasma proteome using a label‐free shotgun UDMSE approach. A total of 859 nonredundant proteins were identified across five biological replicates with stringent identification. Proteins specifically related to sperm maturation and protection, capacitation, fertilization and metabolic activity were detected in the buffalo seminal fluid. In conclusion, we provide a comprehensive proteomic profile of buffalo seminal plasma, which establishes a foundation for further studies designed to understand regulation of sperm function and discovery of novel biomarkers for fertility. MS data are available in the ProteomeXchange with identifier PXD003728. 相似文献
108.
Luis Felipe Falla Zuñiga Yhoiss Smiht Muñoz Cerón Liliana Salazar 《Anatomia, histologia, embryologia》2021,50(1):206-211
Alterations in heart valve development represent more than 20% of congenital cardiovascular malformations. Most of the functional properties of heart valves depend on extracellular matrix. Despite its relevance, little is known about fibrillar components on developing stages. Our objective is to define histological changes on valves fibrillar components in late embryonic development of Mus musculus. We found type III collagen as the predominant fibre type in the ECM in prenatal stages followed by a switch to a type I predominance for postnatal ages. The change in fibrillar components is necessary to support the normal mechanical function of adult heart valves. 相似文献
109.
110.
Felipe García-Oliva Juan F. Gallardo Lancho Noé Manuel Montaño Pilar Islas 《Agroforestry Systems》2006,66(2):93-100
Gains and losses of soil carbon (C), have been reported when tropical forests are converted to pastures. Regional studies
are crucial for setting regional baselines and explaining each particular trend, in order to solve this controversy. Tropical
deciduous forest (TDF) is under high deforestation pressure, mainly for conversion to pastures. The present study compared
soil organic C (SOC) and nitrogen (SON) in the surface layer (0–5 cm) of forest and pasture soils in a TDF of western Mexico.
SOC and SON concentrations were 18 and 60% lower in pasture soils than in forest soils, and C:N ratio increased in pasture
soils. Furthermore, pasture soils had lower labile C and available inorganic nitrogen (N) than forest soils. These results
can be explained as a reduction in C inputs to pasture soils and management-induced disruption of soil aggregates. In forest
soils, macroaggregates (> 250 μm) were predominant (85%), whereas in pasture soils they were reduced to 35% of dry sand-free soil mass. The estimated SOC
and SON losses from the top 5 cm of soil were 3 Mg C ha−1 and 0.9 Mg N ha−1, respectively. 相似文献