1H-NMR Method Enables Early Identification of Degeneration in the Quality of Sweet Potato Tubers

In sweet potato tuber, which is a tropical plant, long‐term storage leads to loss of water and carbohydrate, thus water mobility was investigated using ¹H‐NMR spectroscopy. Electrolyte leakage indicated that tubers stored at 15 °C for 1 year were partly injured and that frozen‐thawed tissues were dead. Nuclear magnetic resonance (NMR) spin–lattice relaxation time (T₁) and spin–spin relaxation time (T₂) clearly increased with the duration of storage, whereas these values decreased in the dead tissues. Furthermore, Arrhenius plots for T₁ and T₂ were determined at temperatures ranging from 20 to 0 °C in 2.5 °C steps. In the fresh tubers, a strong converse temperature dependency was shown in the T₂ measurement. On the contrary, there was no temperature dependency in the T₂ of the dead tissues. Thus, the existence of inverse temperature dependency reflected tissue viability. Additionally, any change in the T₂ of the fresh tubers occurred at about 14 °C, which virtually coincided with the storage temperature of 15 °C. The slope change in T₂ might have responded to a physiological change as a primary event. In conclusion, monitoring water status by NMR could provide early identification of changes in the quality of post‐harvest crops; this method shows great promise for use in environmental‐stressed crop yield research.     

Marketing systems of freshwater prawns in three coastal districts of Bangladesh

The present study analyzes the prawn marketing chain before and after the implementation of a Danish-funded project (GNAEP) in three coastal districts of greater Noakhali, southeast region of Bangladesh. The results reveal that, before GNAEP, there was a longer prawn marketing chain with a number of intermediaries who used to share a considerable amount of market margin. However, after implementation of GNAEP, the prawn marketing chain has become shorter with a significant reduction of intermediaries which facilitates direct selling of prawns to processing plants through local Community Based Organizations (CBOs). The present prawn marketing system in the regions is in favor of prawn farmers. Farmers have greater advantages because 1) CBOs provide reasonable market price and actual weight, 2) CBOs can bargain with processing plants as the former are aware of the latest international market prices through Marketing Information System 3) number of intermediaries is lowered and 4) transport and preservation costs are reduced. The displaced intermediaries, some of whom are powerful persons in the society, have again started to influence the prawn market in different ways. This fact has opened up another discussion on how the power structure is shifting in this region and how they are affecting prawn marketing.     

INVESTIGATIONS ON FOLIAR SPRAY OF BORON AND MANGANESE ON OIL CONTENT AND CONCENTRATIONS OF FATTY ACIDS IN SEEDS OF SUNFLOWER PLANT RAISED THROUGH SALINE WATER IRRIGATION

The objective of this experiment was to evaluate the effect of foliar fertilization of some micronutrients [i.e., manganese (Mn) and boron (B)] on reproductive yield and fatty acid concentrations of a standard sunflower hybrid (‘NuSun 636') irrigated with different concentrations of saline water made by dissolving sea salt. Reproductive yield showed a significant decrease with the increase in salt in the rooting medium. However, foliar sprays of boric acid (H₃BO₃) and manganese chloride (MnCl₂) showed a significant increase in seed number, seed weight, and oil content of seeds in the nonsaline control, which persisted even under saline water irrigation. An increase under the MnCl₂ spray was more than with H₃BO₃ irrespective of non saline or saline water irrigation. Increasing levels of salinity appeared to be responsible for a decrease in oleic monounsaturated fatty acid concentration and an increase in the linoleic polyunsaturated, palmitic and stearic saturated fatty acid content whereas no significant change was found in linolenic polyunsaturated fatty acid content. Foliar applications of H₃BO₃ and MnCl₂ brought some beneficial alteration in fatty acid concentrations of sunflower. Foliar application of H₃BO₃ caused a significant increase in palmitic and stearic saturated fatty acids and linoleic polyunsaturated fatty acids in control as well as under saline conditions. Oleic monounsaturated fatty acid concentration showed a decline under H₃BO₃ treatment irrespective to nonsaline or saline conditions. Foliar applications of MnCl₂ increased the concentration of palmitic saturated fatty acid and oleic monounsaturated fatty acid irrespective to the plant growth under non saline or saline conditions. While stearic saturated fatty acid, linoleic and linolenic polyunsaturated fatty acid decreased with the application of manganese as compared to the non sprayed control.     

多种植物提取物对小菜蛾产卵的驱避作用

小菜蛾仅取食为害十字花科植物，其他非嗜食植物的提取物对小菜蛾可能有好的控制作用，在温室内试验研究了14种常见植物的提取物对小菜蛾雌虫的产卵驱避作用，绝大部分供试植物的提取物可减少小菜蛾的落卵量40％以上，作用最强的是尾叶桉的抽提物，喷施24h后卵降低率达65．52％。植物提取物对小菜蛾雌虫的产卵驱避作用48h后下降，卵减少率最高为飞机草的抽提物，达52．91％，非嗜食植物次生物质在小菜蛾的控制中会有良好的前景。     

Allelic segregation of sex‐linked microsatellite markers in Nile tilapia (Oreochromis niloticus) and validation of inheritance in YY population

Stocking of all‐male fingerling produced by direct administration of male hormone 17‐α‐methyltestosterone is the most preferred method for present‐day aquaculture of the Nile tilapia Oreochromis niloticus. However, due to the growing concern of negative impact of steroid hormone in food fish, production of ‘genetically male’ tilapia, which depends on the concrete and thorough understanding of sex determination, has long been a scientific curiosity. The objective of the present study was to identify reliable sex‐linked markers and to evaluate the applicability of those markers in terms of monosex production approach. ‘XY’ neofemales were produced by using synthetic oestrogen and identified through selective breeding and progeny testing. Three females with progeny not deviating from 3:1 sex ratio (male:female) were designated as ‘XY’ neofemales and were used subsequently to produce putative YY progeny. Among the fifteen microsatellite markers tested, marker ARO172 was most informative in differentiating male and female genotypes. Twenty‐seven F₂ fish from three families were identified as putative YY males based on marker genotyping, and four of them were crossed to produce F₃ to validate marker association by progeny testing. The YY males produced 86%–100% male progeny indicating ARO172 a unique sex‐linked marker applicable in marker‐assisted selection.     

Elevated temperature affects growth and hemato‐biochemical parameters,inducing morphological abnormalities of erythrocytes in Nile tilapia Oreochromis niloticus

Temperature is considered as an important environmental factor, and the increasing water temperature resulting from global warming is a great concern. The present study was conducted to examine the effects of elevated water temperature on growth, hemato‐biochemical parameters in Nile tilapia, Oreochromis niloticus acclimatized to three temperatures (31°C, 34°C and 37°C) for 60 days. Additionally, erythrocytic cellular abnormalities (ECA) and erythrocytic nuclear abnormalities (ENA) tests were assayed using peripheral erythrocytes after exposure to the three temperatures. Fish were sacrificed on days 7, 15, 30 and 60 of exposure. Growth performances viz., weight gain, % weight gain and specific growth rate (SGR) showed decreasing tendency at 34°C but significantly declined at 37°C compared to 31°C. The abundance of haemoglobin (Hb) and red blood cells (RBCs) significantly decreased in response to temperature increases, while white blood cells (WBCs) displayed the opposite response. At days 7 and 15, blood glucose levels significantly increased in response to the temperature increase, while at days 30 and 60 glucose declined. Frequencies of ECA and ENA were significantly enhanced at the highest temperature throughout the experimental period. Dissolved oxygen decreased and free CO₂ increased significantly with increasing temperature throughout the study period. The present study revealed that temperatures higher than 34°C may be hazardous to O. niloticus.     

Antiproliferative and Antimicrobial Potentials of a Lectin from Aplysia kurodai (Sea Hare) Eggs

In recent years, there has been considerable interest in lectins from marine invertebrates. In this study, the biological activities of a lectin protein isolated from the eggs of Sea hare (Aplysia kurodai) were evaluated. The 40 kDa Aplysia kurodai egg lectin (or AKL-40) binds to D-galacturonic acid and D-galactose sugars similar to previously purified isotypes with various molecular weights (32/30 and 16 kDa). The N-terminal sequence of AKL-40 was similar to other sea hare egg lectins. The lectin was shown to be moderately toxic to brine shrimp nauplii, with an LC₅₀ value of 63.63 µg/mL. It agglutinated Ehrlich ascites carcinoma cells and reduced their growth, up to 58.3% in vivo when injected into Swiss albino mice at a rate of 2 mg/kg/day. The morphology of these cells apparently changed due to AKL-40, while the expression of apoptosis-related genes (p53, Bax, and Bcl-XL) suggested a possible apoptotic pathway of cell death. AKL-40 also inhibited the growth of human erythroleukemia cells, probably via activating the MAPK/ERK pathway, but did not affect human B-lymphoma cells (Raji) or rat basophilic leukemia cells (RBL-1). In vitro, lectin suppressed the growth of Ehrlich ascites carcinoma and U937 cells by 37.9% and 31.8%, respectively. Along with strong antifungal activity against Talaromyces verruculosus, AKL showed antibacterial activity against Staphylococcus aureus, Shigella sonnei, and Bacillus cereus whereas the growth of Escherichia coli was not affected by the lectin. This study explores the antiproliferative and antimicrobial potentials of AKL as well as its involvement in embryo defense of sea hare.     

Genetic variability in bread wheat (<Emphasis Type="Italic">Triticum aestivum</Emphasis> L.) of Pakistan based on polymorphism for high molecular weight glutenin subunits

Variation in bread wheat including pre and post green revolutions varieties of Pakistan along with landraces was investigated for high molecular weight Glutenin subunits (HMW Gs) encoded at three genes (Glu-A1, Glu-B1, Glu-D1) with SDS-PAGE. The germplasm was diverse and unique on the basis of HMW Gs compositions and out of 14 alleles detected at all the Glu-1 loci, three belonged to Glu-A1, nine to Glu-B1 and two to Glu-D1 locus. High variation was observed in the landraces and higher gene diversity was observed between the populations as compared to the gene diversity within populations, whereas a reverse pattern of gene diversity was observed when populations were pooled across the region (higher within the regions than between the regions). A lack of relationship between the HMW Gs diversity and the altitude of collection site was observed. A data base has been generated in this study which could be expanded/exploited for cultivar development or management of gene bank.     

Bacillus thuringiensis C25 which is rich in cell wall degrading enzymes efficiently controls lettuce drop caused by Sclerotinia minor

European Journal of Plant Pathology - Sclerotinia is a phytopathogenic genus of fungi that form sclerotia: black, hard, seed-like functioning bodies. Sclerotinia sclerotiorum and Sclerotinia minor...     

Wheat leaf senescence and its regulatory gene network

Wheat leaf senescence is a developmental process that involves expressional changes in thousands of genes that ultimately impact grain protein content (GPC), gr...