Osteopetrosis in a neonatal donkey

Osteopetrosis is a rare disorder characterised by a defect in osteoclastic bone resorption. This report describes osteopetrosis in a neonatal donkey that suffered a displaced tibial fracture. Radiographic examination identified generalised reduction in medullary cavity size, thickened mid‐diaphyseal cortices and conical metaphyseal bone extending toward the mid‐diaphysis of long bones. Postmortem examination identified additional fractures and brittle bones. Histologically, osteoclasts were absent in multiple bone sections. Diaphyseal cortices consisted of concentric bone lamellae with marrow tissue infiltration. Large wedges of secondary spongiosa extended from the metaphyseal growth plate. Clinical and histopathological features were similar to an osteoclast‐deficient, autosomal recessive form of osteopetrosis in humans.     

Effects of Exogenous Insulin on Luteolysis and Reproductive Cyclicity in the Mare

Insulin is a pancreatic hormone that classically regulates carbohydrate and fat metabolism, but also appears to play a role in various reproductive processes. A preliminary study suggested insulin production by day 10 to 18 equine conceptuses. The aim of the present study was to examine the hypothesis that insulin is the conceptus signal responsible for maternal recognition of pregnancy (MRP) in the mare, or otherwise influences reproductive cyclicity during the MRP period. Six Warmblood mares were treated daily during days 7 to 17 after ovulation of two successive oestrous cycles with either (short and intermediate acting) insulin or control saline. Mares were assigned randomly to treatment, and crossed over during the subsequent cycle. Time of ovulation and corpus luteum surface area were determined by serial transrectal ultrasonographic examination of the mares' ovaries, and daily jugular vein blood samples were analysed for progesterone and luteinizing hormone (LH) concentrations. On day 14 of dioestrus, the luteolytic drive was examined by measuring systemic 15-ketodihydroprostaglandin F_{2 α} (PG-metabolite) release in response to oxytocin challenge. In addition, yolk sac fluid recovered from 32 day 10 to 14 equine conceptuses was analysed for insulin concentrations. Insulin administration did not affect luteal size, dioestrus length, the interovulatory interval, or circulating LH concentrations. Insulin administration also failed to suppress oxytocin-induced PGF_{2 α} release, and tended to depress systemic progesterone concentrations. Finally, insulin could not be detected in the yolk sac fluid of day 10 to 14 equine conceptuses by radio-immunoassay. It is concluded that insulin administered daily during days 7 to 17 of dioestrus has little or no effect on reproductive cyclicity in the mare, and is unlikely to be the MRP signal.     

Vegetation community responses to different establishment regimes in loblolly pine (Pinus taeda) plantations in southern MS,USA

Management treatments involving multiple herbicide applications are standard procedure on most industrial pine plantation sites in the southern USA, raising concerns about biodiversity impairment. Management decisions impact not only plant communities but also the habitat potential they create for wildlife. We tested the effects of five intensities of stand establishment treatments on vegetation communities in loblolly pine plantations (n = 4) to age 5 in the Middle Coastal Plain (MCP) of Mississippi. Measurements were species richness, diversity, coverage, and community composition. Treatments were combinations of mechanical site preparation (MSP), chemical site preparation (CSP), and herbaceous weed control (HWC) both banded or broadcast for 1 or 2 years using the same herbicide mixtures. Tree richness and diversity were reduced by increasing treatment intensity; tree coverage, which included crop and non-crop trees, was less in moderate-intensity treatments. Vine richness and coverage were less in more intensive treatments, but 2 diversity indices differed on whether vine diversity was likewise affected. Richness and coverage of forbs and graminoids was lessened by broadcast HWC, with effects mostly limited to the year of application. Plant communities differed in all 5 years, with CSP acting as the primary factor for years 2–5. Early seral communities were favored by CSP, but broadcast HWC suppressed resulting herbaceous plants. Though CSP may somewhat reduce stand-level plant diversity, it may increase overall biodiversity within plantation-dominated landscapes by creating early succession plant communities that enhance wildlife habitat.     

Pulmonary alveolar microlithiasis in an alpaca (Vicugna pacos)

Pulmonary alveolar microlithiasis (PAM) is a rare, familial disease of unknown aetiology characterised by intra‐alveolar formation and accumulation of microliths. Multiple formalin‐fixed tissues were submitted from a 5‐month‐old female alpaca that died suddenly without significant clinical signs. No gross abnormalities were observed on postmortem examination. Histological findings included PAM and severe centrilobular hepatic necrosis. Although the hepatic lesion was the likely cause of death, PAM was an incidental finding that has not been reported previously in alpacas. An overview of PAM, including pathogenesis and histopathological characteristics, are discussed in relation to the concurrent hepatic disease in the present case.     

Sperm Pre-Incubation Prior to Insemination Affects the Sex Ratio of Bovine Embryos Produced in vitro

The objective of the present study was to determine whether sperm incubation prior to oocyte insemination in vitro affects the sex ratio of resulting blastocyst. Cumulus–oocyte‐complexes (COCs) collected from slaughterhouse ovaries were matured in vitro and inseminated with frozen‐thawed semen of three proven artificial insemination (AI) bulls pre‐incubated in vitro in Sperm‐Talp for 6 and 24 h. On day‐9 blastocysts were collected and processed for sex determination. More than 80% of blastocyst were successfully sexed. There were no significant differences in cleavage and blastocyst rates using sperm pre‐incubated for 6 h as compared with the 0‐h pre‐incubation control group. The cleavage and blastocyst rates were significantly lower in the 24‐h pre‐incubation group. The male to female ratio, when compared with the theoretical 1 : 1, differed significantly in favour of females among hatched (viable) blastocysts derived from sperm pre‐incubated for 24 h prior to insemination as well as among all blastocytsts in the 6‐h group. Moreover, when the sperm treatment was considered, the sex ratio was affected only among hatched blastocysts in 24‐h pre‐incubation group. It was concluded that prolonged sperm pre‐incubation influences the rate of development and the sex ratio among hatched blastocysts.     

Preclinical Pharmacology of BA-TPQ,a Novel Synthetic Iminoquinone Anticancer Agent

Marine natural products and their synthetic derivatives represent a major source of novel candidate anti-cancer compounds. We have recently tested the anti-cancer activity of more than forty novel compounds based on an iminoquinone makaluvamine scaffold, and have found that many of the compounds exert potent cytotoxic activity against human cancer cell lines. One of the most potent compounds, BA-TPQ [(11,12),7-(benzylamino)-1,3,4,8-tetrahydropyrrolo[4,3,2-de]quinolin-8(1H)-one], was active against a variety of human cancer cell lines, and inhibited the growth of breast and prostate xenograft tumors in mice. However, there was some toxicity noted in the mice following administration of the compound. In order to further the development of BA-TPQ, and in a search for potential sites of accumulation that might underlie the observed toxicity of the compound, we accomplished preclinical pharmacological studies of the compound. We herein report the in vitro and in vivo pharmacological properties of BA-TPQ, including its stability in plasma, plasma protein binding, metabolism by S9 enzymes, and plasma and tissue distribution. We believe these studies will be useful for further investigations, and may be useful for other investigators examining the use of similar compounds for cancer therapy.     

Carbon allocation and morphology of cherrybark oak seedlings and sprouts under three light regimes

• ? Continued problems in regenerating oak forests has led to a need for more basic information on oak seedling biology.
• ? In the present study, carbon allocation and morphology were compared between cherrybark oak (Quercus pagoda Raf.) seedlings and sprouts at 1-Lag grown in full, 47%, and 20% sunlight.
• ? Results indicated that cherrybark oak seedling carbon allocation and morphology responded plastically to light availability. In full light, roots were sinks for ¹⁴C, while shoots were sinks for ¹⁴C under reduced light availability. Cherrybark oak sprouts exhibited similar carbon allocation patterns in response to light availability, but displayed stronger shoot sinks than seedlings when grown underreduced light availability. We also showed that young oak sprout roots are a sink for ¹⁴C-photosynthates.
• ? Results from this study point to the need for a morphological index for oak sprout development so more precise comparisons in sprout development and physiology can be made with seedlings.

     

Alternative eucalypts for commercial pulpwood production at moderately dry sites in the warm temperate zone of KwaZulu-Natal,South Africa

During 2001, a series of four site–taxa interaction trials was established in the warm temperate (mean annual temperature range 16–19 °C) forestry climatic zone of KwaZulu-Natal (KZN), South Africa. The main objective was to investigate the commercial forestry potential of a range of alternative Eucalyptus and Corymbia taxa for moderately dry (mean annual precipitation < 850 mm) sites within the zone. At rotation end, all trials received final standing tree measurements. At one of the sites (Rockvale), trees of eight promising alternative taxa were felled, stem measurements and wood samples taken, and wood and fibre productivities determined. On the basis of tree growth and wood and fibre production, C. citriodora subsp. variegata, C. henryi, E. badjensis, E. benthamii and E. dorrigoensis (listed alphabetically) demonstrated the greatest potential as commercial alternatives for moderately dry sites in the KZN warm temperate zone. The majority of these taxa showed strong site specificity. Eucalyptus benthamii was the only taxon showing high growth adaptability to the entire range of site conditions applied in the series. The non-susceptibility of C. citriodora subsp. variegata and C. henryi to the current topical insect pests Thaumastocoris peregrinus, Leptocybe invasa and Glycaspis brimblecombei elevates the appeal of these taxa as potential alternative planting choices for moderately dry sites within lower altitude (<1 100 m asl) areas of the climate zone. The matching of the most promising alternative taxa in the series to the range of sites encountered in moderately dry areas of the KZN warm temperate forestry zone is discussed in this paper.