Embryonation and infectivity of Ascaris suum eggs. A comparison of eggs collected from worm uteri with eggs isolated from pig faeces

Ascaris suum eggs were collected from pig faeces or dissected from worms obtained from the same pigs. Eggs from the two sources were allowed to embryonate in 0.1 N H2SO4, in 1% buffered formalin or in tap water. The embryonation of the sulphuric acid and water cultures occurred at the same speed, while the formalin cultures developed slightly more slowly. By experimental inoculation of helminth-free pigs and subsequent counting of white spots in the livers and larvae in the lungs day 7 p.i., the infectivity of eggs dissected from worm uteri and embryonated in sulphuric acid (a normal laboratory procedure) was compared with that of eggs collected from faeces and embryonated in water (i.e. more naturally developed eggs). The results suggest that the two types of eggs were equally infective. For this reason the common practice of using Ascaris eggs dissected from worms for experimental infections might be acceptable.     

Ectomycorrhizal fungal resources of Inner Mongolia Autonomous Region: above ground macrofungi and belowground ectomycorrhizae

New Forests - Desertification and land use pressures reduced forest cover in Inner Mongolia by 3% between 1992 and 2001. These forests primarily form symbioses with ectomycorrhizal fungi, and...     

Transformation of even-aged European beech (Fagus sylvatica L.) to uneven-aged management under changing growth conditions caused by climate change

Transformation from even-aged to uneven-aged forest management is currently taking place throughout Europe. Climate change is, however, expected to change growth conditions—possibly quite radically. Using a deterministic approach, it was the objective of this study to investigate the influence of such changes on optimal transformation strategies for an even-aged stand of European Beech in Denmark. For a range of growth change scenarios, represented by changes in site index, optimal harvest policies were determined using a matrix modelling approach and a differential evolution algorithm. Transition probabilities were updated continuously based on stand level variables and the transition matrix was thus dynamic. With optimal transformation policies, stand development followed similar pathways during the transformation phase irrespective of climate change scenario. Optimal transformation policies were thus robust, suggesting that a good policy would work well under different outcomes of climate change, i.e., acting under erroneous assumptions about change would not lead to major economic loss. For the chosen case stand, the net present value (NPV) of the transformation phase (first 100 years) contributed 80–90 % of the total expectation value at a 2 % discount rate. To assess the robustness of the optimisation procedure and understand the nature of the response surface, 100 replications per scenario were carried out. Variation between replications peaked during the later stages of the transformation phase indicating several pathways for transformation, which were characterised by almost identical levels of profitability.     

The reproductive success of a Quercus petraea × Q. robur F1-hybrid in back-crossing situations

A 56 year old Quercus petraea × Q. robur F1-hybrid was back-crossed to both parental species. Pollen mixes were applied and paternity assigned to offspring based on microsatellite markers. The studied Q. petraea × Q. robur hybrid proved highly fertile and back-crossed well with both Q. robur and Q. petraea with slight but not significant preference for Q. robur. The results do not support the hypothesis about highly unidirectional gene flow between Q. robur and Q. petraea towards Q. robur as the observed back-crossing ability of the hybrid opens a route for nuclear gene flow from Q. robur to Q. petraea. However, Q. petraea × Q. robur hybrids may be rare in nature and the results do not tell us if the (probably more common) reciprocal hybrid also back-crosses easily to Q. petraea.     

Moisture-dependent elastic characteristics of walnut and cherry wood by means of mechanical and ultrasonic test incorporating three different ultrasound data evaluation techniques

Ultrasonic testing is a non-destructive testing method of choice for estimating the anisotropic elastic properties of wood materials. This method is reliable for estimating the Young’s and shear moduli. However, its applicability to Poisson’s ratios remains uncertain. On the other hand, despite their destructive nature, mechanical tests allow a direct measurement of all elastic properties including the Poisson’s ratios. In some cases (e.g. when assessing cultural heritage objects), destructive testing may not be an option. In this work, two types of hardwood walnut (Juglans regia L.) and cherry (Prunus avium L.), which often appear on cultural heritage objects, were tested using both ultrasonic and mechanical testing methods under four different moisture conditions below fibre saturation point. The results show that a higher moisture condition leads to a decrease in material elasticity. For walnut wood, their longitudinal Young’s modulus (\(E_{\rm L}\)) was reduced by 679 MPa under the compression load for a one per cent increase in moisture content. Moreover, three ultrasound data evaluation techniques, which differ in the way they incorporate the Poisson’s ratios (full stiffness inversion, simplified uncorrected, and simplified corrected), were used to estimate the Young’s moduli (E). The main goal is to obtain reliable material parameters using the ultrasound test. As a result, it is concluded that the chosen data evaluation method influences the accuracy of the calculated E. In a certain case, the simplified-corrected method, which requires only one specimen type, gave a closer agreement to mechanical tests (e.g. \(\Delta E_{\rm T}=6\,\%\) deviation on mechanical results). In another case, the full-stiffness-inversion method, which requires four specimen types, gave the best estimation (e.g. \(\Delta E_{\rm L}=2\,\%\)). In this corresponding direction, the simplified-corrected method can only partially reduce the overestimation of the simplified uncorrected from \(\Delta E_{\rm L}=47\) to 32 %. The variation of E produced by different evaluation procedures is due to the different correction factor values, which is a consequence of the variation in \(\nu\).     

The effects of temporal differences between map and ground data on map-assisted estimates of forest area and biomass

Key message

When areas of interest experience little change, remote sensing-based maps whose dates deviate from ground data can still substantially enhance precision. However, when change is substantial, deviations in dates reduce the utility of such maps for this purpose.

Context

Remote sensing-based maps are well-established as means of increasing the precision of estimates of forest inventory parameters. The general practice is to use maps whose dates correspond closely to the dates of ground data. However, as national forest inventories move to continuous inventories, deviations between map and ground data dates increase.

Aims

The aim was to assess the degree to which remote sensing-based maps can be used to increase the precision of estimates despite differences between map and ground data dates.

Methods

For study areas in the USA and Norway, maps were constructed for each of two dates, and model-assisted regression estimators were used to estimate inventory parameters using ground data whose dates differed by as much as 11 years from the map dates.

Results

For the Minnesota study area that had little change, 7-year differences in dates had little effect on the precision of estimates of proportion forest area. For the Norwegian study area that experienced considerable change, 11-year differences in dates had a detrimental effect on the precision of estimates of mean biomass per unit area.

Conclusions

The effects of differences in map and ground data dates were less important than temporal change in the study area.