不同药剂对水木坚蚧防治效果

为探索广玉兰水木坚蚧防治方法,本文设计13个处理对广玉兰水木坚蚧化学药剂喷雾试验,不同处理后14天后调查统计分析,蚧必治800倍液、蚧必治600倍液、吡虫啉400倍液、蚧必治与阿维菌素(3∶2)复配剂防治效果明显较好,死亡率分别是99.21%、96.94%、88.92%、95.73%,防治效果分别是99.09%、96.49%、87.33%、95.11%。在蚧必治内添加阿维菌素对广玉兰水木坚蚧防治效果具有促进作用。     

纳米水雾环境对新鲜农产品采后贮藏过程中失重的调控

质量损失与冷害症状在新鲜农产品冷藏过程中经常发生,并会导致园艺产业巨大的经济损失。本试验分别研究了三种园艺产品,茄子(Solanum melongena)、日本芜青(Brassica rapa)和无花果(Ficus carica)在用两种类型加湿器制造的相对湿度为95%、温度分别为5.5℃和7℃的环境下贮藏10、6和8 d的贮藏品质。由纳米加湿器生成的水雾粒子平均直径小于100 nm,而由超声波加湿器生成的水雾粒子平均直径为216 nm。结果表明,纳米加湿器处理的日本芜菁、茄子和无花果样品的失重率分别为3.7%、5.3%和8.8%,超声波加湿器处理三种样品的失重率分别为7.3%、8.5%和14.7%。在纳米水雾贮藏室内放置的茄子果实的冷害指数要低于在超声波加湿环境中贮藏的果实。纳米水雾中的日本芜菁和无花果的气孔相比其初始状态下分别关闭了34.7μm2和51.5μm2,而在超声波水雾中则分别关闭了15.8μm2和25.5μm2。在纳米水雾中贮藏的日本芜菁的颜色要比在超声波水雾中贮藏的芫菁更绿。     

In vitro production of porcine embryos: current status,future perspectives and alternative applications

The pig is considered to be a suitable source of cells and organs for xenotransplants, as well as a transgenic animal to produce specific proteins, given the biological similarities it shares with human beings. However, the in vitro embryo production system in pigs is inefficient compared with those in other mammals, such as cattle or mice. Although numerous modifications have been applied to improve the efficiency of in vitro embryo production systems in pigs, not much progress has been made to overcome the problem of polyspermy, and low developmental ability due to insufficient cytoplasmic abilities of in vitro matured oocytes and improper culture conditions for the in vitro produced embryos. Recent achievements, such as the establishment of chemically defined medium and utilization of ‘zona hardening’ technique, have gained some success. However, further research for the reduction of polyspermy and detrimental effects of the culture systems in pigs is still needed.     

Calibrating fixed- and mixed-effects taper equations

Accurate and affordable measurements of upper-stem diameters are now possible thanks to recent advances in laser technology. Measurement of the midpoint upper-stem diameter can be employed to improve the accuracy of diameter predictions along the tree bole. Felled-tree data from a loblolly pine (Pinus taeda L.) plantation was used to evaluate two approaches: (1) calibrating a segmented taper equation by constraining a parameter, and (2) localizing the taper model by predicting the random effects for each tree. The calibration technique is much simpler and produced less-biased prediction of diameters and is therefore recommended. Calibration results were similar for both fixed- and mixed-effects taper models, even though a slight gain in accuracy and precision was attained with the mixed-effects model.     

Integrated freshwater aquaculture,crop and livestock production in the Mekong delta,Vietnam: Determinants and the role of the pond

Promotion of integrated aquaculture with agriculture, including crops and livestock (IAA-farming), requires consideration of both bio-physical and socio-economic contexts. The major factors influencing the adoption of IAA-farming by households at three sites in the Mekong delta were identified. Special attention was given to the multiple roles ponds play in IAA-farming systems. Information was collected through semi-structured interviews and discussions with focus groups and key individuals. Data were analyzed using multivariate factor analysis, analysis of variance or participatory ranking methods. Three major IAA-systems were identified: (1) low-input fish farming integrated with intensive fruit production (system 1), (2) medium-input fish farming integrated with less intensive fruit production (system 2), and (3) high-input fish farming integrated with less intensive fruit production (system 3). System 1 was commonly practised in a rural fruit-dominated area with fertile soils, while systems 2 and 3 were more evident in peri-urban rice-dominated areas with less fertile soils. In the study area, only 6% of poor farmers adopted IAA-farming, while this was 42% for intermediate and 60% for rich households. Richer farmers tended to intensify fish farming and seek a more commercial orientation. The major factors why farmers did not start aquaculture were the inappropriateness of technology, insufficient land holding or poor access to extension services, limited farm management, and through a fear of conflicts associated with pesticide use on crops. The main motivations for practising IAA-farming included increased income and food for home consumption from the available farm resources while reducing environmental impacts. Further improvements to IAA-systems can be realized by strengthening nutrient recycling between different IAA-system components while enhancing farming output and safeguarding the environment.     

Biology and management of barnyardgrass,red sprangletop and weedy rice

About 400 weed species of 73 families have been reported to occur in upland and lowland rice fields in Vietnam. Two important families are Poaceae and Cyperaceae having 42% of weed species with 21% each. The barnyardgrass, Echinochloa crus‐galli (L.) Beauv., is the most important weed in both transplanted and direct‐seeded rice in this country. The competi‐tion of 25 barnyardgrass plants/m² causes approximately 50% yield loss. Red sprangletop, Leptochloa chinensis (L.) Nees., is an emerging major weed in direct‐seeded rice. Weedy rice (Oryza sativa L.) was detected in 1994 and is now a new dangerous pest in rice fields. The main reasons are: herbicides which can kill weedy rice completely are not available in the market, non‐chemical methods are laborious and costly, and weedy rice can develop a new generation from contaminated seeds through rice sowing or emerging from soil seed bank. Research results on the Vietnamese situation of barnyardgrass, red sprangletop and weedy rice in terms of biology and management by chemical and non‐chemical methods have been reviewed in this paper.     

Estimation of the standardized ileal digestible lysine requirement and optimal sulphur amino acids to lysine ratio for 30–50 kg pigs

Two experiments were conducted to determine the standardized ileal digestible (SID) lysine (Lys) requirement and the ideal SID sulphur amino acids (SAA) to Lys ratio for 30–50 kg crossbred pigs. In experiment 1, a total of 72 crossbred pigs with an average initial body weight (BW) of 28.9 kg were allotted to one of six dietary treatments in a randomized complete block design. Each diet was assigned to six pens containing two pigs each. Six diets were obtained by supplementing graded levels of L‐Lysine?HCl to create six dietary levels of SID Lys (0.70%, 0.80%, 0.90%, 1.00%, 1.10% and 1.20%). Responses of weight gain (ADG) and gain:feed (G:F) to increasing the SID Lys content of the diet fitted well with the curvilinear‐plateau model; whereas, for plasma urea nitrogen (PUN) two‐slope linear broken‐line model was well fitted. The optimal SID Lys requirement for the pigs of this period was 1.10%. Experiment 2 was a dose–response study using SID Met+Cys to Lys ratios of 50%, 55%, 60%, 65%, 70% and 64%. A total of 72 crossbred pigs with initial BW of 32.9 kg were randomly allotted to receive one of the six diets. Diets 1–5 were formulated to contain 1.0% SID Lys to be second limiting in Lys and diet 6 contained 1.11% SID Lys to be adequate in Lys. The average optimal SID SAA:Lys ratio for maximal ADG and G:F and minimal PUN was 65.2% using curvilinear‐plateau and linear broken‐line models.     

Prevalence of Neospora caninum and bovine viral diarrhoea virus in dairy cows in Southern Vietnam

The main purpose of this study was to investigate the prevalence of Neospora caninum and bovine viral diarrhoea virus (BVDV) in some dairy herds in Southern Vietnam, and to ascertain whether there were differences in seroprevalences between herds with imported and locally bred cows. Serum samples collected on five state farms and 97 smallholder herds were analysed for the presence of antibodies to N. caninum and BVDV. All BVDV antibody-negative sera were further tested by antigen-ELISA in order to identify persistently infected individuals. The N. caninum prevalence varied between 16% and 53% in the state herds, and was higher in the four herds that had imported cows than in the herd that only had locally bred cows. Nineteen percent of the samples collected on smallholder farms, which all had only locally bred cows, had antibodies to N. caninum. The BVDV seroprevalence varied between 58% and 93% on the state farms. In smallholder herds, the prevalence of BVDV among the sampled cows was 18% and even lower on the state farms. Despite the high seroprevalence for BVDV in the state herds, no persistently BVDV infected cows were found. Given the high prevalence for Neospora and BVDV among herds with imported cows, it seems advisable to test for both infections before cattle are imported into the country.     

The role of rpoS,hmp, and ssrAB in Salmonella enterica Gallinarum and evaluation of a triple-deletion mutant as a live vaccine candidate in Lohmann layer chickens

Salmonella enterica Gallinarum (SG) causes fowl typhoid (FT), a septicemic disease in avian species. We constructed deletion mutants lacking the stress sigma factor RpoS, the nitric oxide (NO)-detoxifying flavohemoglobin Hmp, and the SsrA/SsrB regulator to confirm the functions of these factors in SG. All gene products were fully functional in wild-type (WT) SG whereas mutants harboring single mutations or a combination of rpoS, hmp, and ssrAB mutations showed hypersusceptibility to H₂O₂, loss of NO metabolism, and absence of Salmonella pathogenicity island (SPI)-2 expression, respectively. A triple-deletion mutant, SGΔ3 (SGΔrpoSΔhmpΔssrAB), was evaluated for attenuated virulence and protection efficacy in two-week-old Lohmann layer chickens. The SGΔ3 mutant did not cause any mortality after inoculation with either 1 × 10⁶ or 1 × 10⁸ colony-forming units (CFUs) of bacteria. Significantly lower numbers of salmonellae were recovered from the liver and spleen of chickens inoculated with the SGΔ3 mutant compared to chickens inoculated with WT SG. Vaccination with the SGΔ3 mutant conferred complete protection against challenge with virulent SG on the chickens comparable to the group vaccinated with a conventional vaccine strain, SG9R. Overall, these results indicate that SGΔ3 could be a promising candidate for a live Salmonella vaccine against FT.     

Production of Ban miniature pig embryos by in vitro fertilization: A comparative study with Landrace

Ban is an endangered miniature pig breed in Vietnam. This study aimed to set up an in vitro embryo production (IVP) system for this breed. Ban's epididymal sperm concentration (1240 ± 35 × 10⁶/mL) was lower (P < 0.01) compared with Landrace (4160 ± 42 × 10⁶/mL). However, sperm characteristics before and after freezing in Ban and Landrace were similar. The numbers of follicles with diameter larger than 2 mm per ovary in Ban females treated with equine chorionic gonadotropin and human chorionic gonadotropin (27.1 ± 1.3) were higher (P < 0.05) than those in Landrace (12.9 ± 2.0) and in non‐hormone stimulated Ban (no > 2 mm follicles). After in vitro maturation, the percentages of oocytes with expanded cumulus cells and the first polar body (matured oocytes) were not different among Ban, hormone‐stimulated Ban and Landrace. The percentages of two‐cell embryos and morulae derived from oocytes collected from three sources did not differ. However, the rate of blastocysts derived from oocytes in non‐stimulated Ban (4.0 ± 3.8%) was lower (P < 0.05) than that in Landrace (15.3 ± 1.8%). In conclusion, an effective IVP system for good quality embryos in Ban, that is essential for genetic conservation of this breed, was established.