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991.
Purpose
Stimulating microbial degradation is a promising strategy for the remediation of soils contaminated with polycyclic aromatic hydrocarbons (PAHs). To better understand the functional microbial populations and processes involved in pyrene biodegradation in situ, the dynamics of pyrene degradation and functional microbial abundance were monitored during pyrene incubation in soils. We hope our findings will provide new insights into in situ pyrene biodegradation in soils and help to identify functional microbes from soils.Materials and methods
Pyrene (60 mg kg?1) was incubated with two different soils, one is lower PAH-containing agricultural soil (LS), and the other is higher PAH-containing industrial soil (HS). During incubation, triplicate samples were collected on days 0, 3, 7, 14, and 35. Pyrene in soil samples was analyzed using an Agilent gas chromatograph (7890A) equipped with a mass-selective detector (model 5897). DNA in soils was extracted with a FastDNA Spin kit for soil (Bio101, USA). The abundance of functional microbes and genes was monitored by a Taqman or SYBR Green based real-time PCR quantification using an iCycler iQ5 themocycler (Bio-Rad, USA). The diversity of PAH-RHDα GP genes was evaluated by constructing clone libraries and sequencing.Results and discussion
In both soils, more than 80 % of the added pyrene was degraded within 35 days. After 35-day incubation, there was a significant enrichment of Gram-positive bacteria harboring PAH-ring hydroxylation dioxygenase (PAH-RHDα GP) genes, and the abundance of Mycobacterium increased significantly. In PAH-RHDα GP clone libraries from two soils, Mycobacterium was detected, while most sequences were closely related to uncultured Gram-positive bacteria. In addition, two pyrene catabolic pathways might be involved in pyrene degradation, as pyrene dioxygenase genes, nidA and nidA3, were dramatically enriched during incubation. Moreover, the abundance and diversity of potential degraders in two soils showed significantly difference in responding to pyrene stress. This result indicates that soil condition can significantly affect functional microbial populations and biological process for pyrene biodegradation.Conclusions
These results revealed that Mycobacterium as well as uncultured Gram-positive PAH-RHDα genotypes may be the important group of pyrene degraders in soils, and two pyrene catabolic pathways, targeted by nidA and nidA3, might potentially contribute to in situ biodegradation of pyrene. This study characterized the response pattern of potential pyrene degraders to pyrene stress in two different soils, which would increase our understanding of the indigenous processes of pyrene biodegradation in soil environment.992.
定期公告水土保持监测情况是水土保持法等法律法规的主要规定之一,但如何利用监测站点的现测数据较为科学准确地推算区域土壤流失量及水土保持效益,是目前水土保持公告中存在的主要问题之一。以北京市为例,利用全市水土保持监测网络站点观测到的降雨量及水土流失量等资料,基于GIS平台和降雨侵蚀力模型,对2009年北京市土壤流失量进行定量计箅。结果表明,计箅结果可靠,方法可行,可为其他地区计箅土壤侵蚀量提供参考。 相似文献
993.
不同因子对青稞类黄酮提取率的影响 总被引:1,自引:0,他引:1
采用乙醇的水溶液为溶剂,青稞粉为原料来浸提青稞类黄酮,研究了乙醇体积分数、提取温度、提取时间、料液比对青稞类黄酮提取率的影响,得出最佳提取条件为:乙醇体积分数为75%,料液比为1:15,浸提温度为40℃,浸提时间1h。 相似文献
994.
995.
Xia P Liu Y Liu X Zhang Z Duan E Lu X Zhao J Cui B 《Veterinary immunology and immunopathology》2011,141(1-2):144-150
Receptors for the Fc fragment of IgG (FcγRs) constitute one of the main effector mechanisms through which IgG immune complexes exert their action. Four FcγRs, FcγRI (CD64) with high affinity, FcγRI with intermediate affinity, FcγRII (CD32) and FcγRIII (CD16) with low affinity, have been identified. There are three FcγRII isoforms (activating FcγRIIa and FcγRIIc, and inhibiting FcγRIIb) existing in humans, one isoform in mice (inhibiting FcγRIIb), and two isoforms in cattle (inhibiting FcγRIIb, activating FcγRIIc). Two splice sub-isoforms of FcγRIIb, FcγRIIb1(b1) and FcγRIIb2(b2), have been identified in humans, mice and cattle, however, few of FcγRIIb sub-isoforms have been investigated in pig. In this study, we describe the molecular cloning, sequencing and characterization of a porcine FcγRIIb sub-isoform, FcγRIIb1. The cDNA encoding porcine FcγRIIb1 was isolated from peripheral blood leucocytes RNA with RT-PCR. The porcine FcγRIIb1 cDNA contains a 951bp open-reading frame, encoding a 316 amino acid transmembrane glycoprotein composed of two immunoglobulin (Ig)-like extracellular domains, a transmembrane region and a cytoplasmic tail with an immunoreceptor tyrosine-based inhibiting motif (ITIM). The porcine FcγRIIb1 shares 98.3% homology and has a 19 amino acid in-frame insertion in cytoplasmic tail when compared with amino acid sequence of DQ026064. Immunofluorescence analysis showed that the glycoprotein encoded by the porcine FcγRIIb1 cDNA was expressed in the stable transfected COS-7 cells, and an immunoglobulin-binding assay showed that it had binding activity for IgG immune complexes. Identification of the porcine FcγRIIb1 will help our understanding of the molecular basis of IgG-FcγR interaction in the porcine immune response. 相似文献
996.
The effects of soil disturbance and residue retention on the functionality of the symbiosis between medic (Medicago truncatula L.) and arbuscular mycorrhizal fungi (AMF) were assessed in a two-stage experiment simulating a crop rotation of wheat (Triticum aestivum L.) followed by medic. Plants were inoculated or not with the AMF, Glomus intraradices and Gigaspora margarita, separately or together. The contribution of the arbuscular mycorrhizal (AM) pathway for P uptake was determined using 32P-labeled soil in a small hyphal compartment accessible only to hyphae of AMF. In general AM colonization was not affected by soil disturbance or residue application and disturbance did not affect hyphal length densities (HLDs) in soil. At 4 weeks disturbance had a negative effect on growth and phosphorus (P) uptake of plants inoculated with G. margarita, but not G. intraradices. By 7 weeks disturbance reduced growth of plants inoculated with G. margarita or AMF mix and total P uptake in all inoculated plants. With the exception of plants inoculated with G. margarita in disturbed soil at 4 weeks, the AM pathway made a significant contribution to P uptake in all AM plants at both harvests. Inoculation with both AMF together eliminated the negative effects of disturbance on AM P uptake and growth, showing that a fungus insensitive to disturbance can compensate for loss of contribution of a sensitive one. Application of residue increased growth and total P uptake of plants but decreased 32P in plants inoculated with the AMF mix in disturbed soil, compared with plants receiving no residue. The AMF responded differently to disturbance and G. intraradices, which was insensitive to disturbance, compensated for lack of contribution by the sensitive G. margarita when they were inoculated together. Colonization of roots and HLDs in soil were not good predictors of the outcomes of AM symbioses on plant growth, P uptake or P delivery via the AM pathway. 相似文献
997.
998.
基于单片机技术的温室自动控制系统的设计 总被引:3,自引:0,他引:3
随着我国农业科技的发展,温室自动控制技术越来越引起人们的关注,成为未来优质高效设施农业的发展趋势。为此,通过市场调研,采用单片机控制技术,进行了温室自动化控制系统的设计开发。通过性能测试实验,取得较好的效果,为将单片机技术引入到温室自动控制系统的后续研究提供了借鉴。 相似文献
999.
利用多体动力学分析软件adams/car,建立了麦弗逊前悬架虚拟样机仿真模型.采用轮跳和静载侧向力加载的方法,对汽车在弯道行驶过程中的K&C特性及其对整车稳态转向特性的影响展开分析研究.仿真结果表明,模型具有较好的不足转向特性,为预测整车稳态转向特性提了仿真与理论依据. 相似文献
1000.
基于仙农熵的土壤多样性和土地利用多样性关联评价 总被引:12,自引:0,他引:12
以南京市为例,将多样性理论与方法应用于土壤和土地利用相关性评价中,对该市1986年和2003年的遥感数据进行了土地利用分类,计算了各土壤类型和各土地利用类型的构成组分多样性指数以及多网格尺度下的空间分布多样性指数,并提出一种用于评价土壤和土地利用相互关系的关联系数,求算了5 km网格尺度下南京市典型土种单元同9种土地利用类型之间的关联系数。研究结果表明,1986年至2003年这十余年间,南京市土地利用的构成组分变化明显,其中1986年的构成组分多样性为0.361,2003年为0.444。2003年南京市土地利用的空间分布离散性更高,5种主要土地利用类型的空间分布多样性均有明显增加。南京市典型土种与各土地利用类型关联系数的计算结果表明,多组关联系数的变化趋势相同,由此推断相比于1986年,2003年南京市土壤与土地利用之间的相互联系更为复杂多变。 相似文献