一种高质量天门冬基因组DNA提取方法

针对天门冬[Asparagus cochinchinensis(Lour.)Merr.]成熟叶片富含多糖、多酚、蛋白质及其他次生代谢物质的特点,采用改良CTAB法对其基因组总DNA进行提取,并对DNA进行质量检测。结果表明,改良CTAB法可从天门冬成熟叶片中获得纯度高、完整性好的总DNA,其OD260 nm/OD280 nm为1.86,OD260 nm/OD230 nm为2.36,浓度为390μg/mL,产率达11.7μg/g,多糖、多酚和RNA杂质被去除干净,无降解现象,说明该方法提取的天门冬基因组总DNA质量较高。     

农药除蜗灵对土壤微生物的影响

指出了农药除蜗灵近年来的大量使用,对土壤环境质量产生了一定的影响,进行了其对土壤-植物根际微生物数量上的影响的试验,并设计了农药浓度和施药时间的不同,从而了解了农药除蜗灵对微生物的长远影响。为农药除蜗灵的施用时间、施用浓度及其对土壤环境质量的影响提供了一定的科学依据。通过采用倾注法分离土壤中微生物,利用平板计数法进行计数,可得知农药除蜗灵对土壤中微生物的影响如下:对细菌和放线菌都产生了明显的促进作用,而对霉菌产生了显著的抑制作用。随着时间的推移,农药除蜗灵对放线菌的增加作用最先减弱,霉菌次之,细菌最晚恢复,这又说明了土壤本身对农药具有一定的净化作用。     

黄河流域机采棉植株性状的筛选

为筛选适合黄河流域棉区机采的品种,对16份棉花材料的果枝始节高度、株高、产量构成因素、纤维品质指标进行系统调查、分析与研究。结果表明:适合进一步培育机采品种的材料为590032、中棉所49。     

冬闲地种草养鹅模式下不同鹅品种生长性能、屠宰性能、肉品质的比较

为比较冬闲地种草养鹅模式下不同鹅品种的生长发育、屠宰性能和肉品质,以28日龄四川白鹅、浙东白鹅、扬州鹅和霍尔多巴吉鹅为研究对象,采取全程草地放牧加补饲方式,每组3个重复,每个重复10只,于70日龄开展屠宰测定和肉品质测定。结果显示:①在整个生长发育期间,四个品种鹅在生长发育前期增重较大,后期趋于缓慢。在生长发育前期(28~49日龄),扬州鹅的周增重均不同程度地高于四川白鹅和浙东白鹅,但在36~42日龄时扬州鹅的体增重低于霍尔多巴吉鹅;在生长发育后期(50~70日龄),扬州鹅的体增重均高于其他三个品种鹅。②扬州鹅的屠宰率高于其他三个品种的鹅;扬州鹅的腹脂率为1.39%,显著低于其他三个品种鹅。③扬州鹅不同部位肌肉中水分含量最低,胶原含量最高;扬州鹅的胸肌中蛋白质含量显著高于其他三个品种鹅。综上所述,扬州鹅可作为种草养鹅的理想品种,研究结果为种草养鹅模式下适宜品种的选择提供了理论依据。     

Development and Application of Cotton InDel Markers Based on High Throughput Sequencing

[Objective] Dimorphic InDel markers can be used for cotton variety identification and purity detection, to improve the accuracy and efficiency of cotton seed testing, and to play a role in molecular breeding of cotton. [Method] Based on the whole genome sequencing of 121 cotton varieties from different sources, the InDel markers with high polymorphism were developed according to polymorphism information content(PIC) and were applied in the genetic distance analysis and cluster analysis by using 66 cotton varieties in China. [Result] Totally 10 967 InDel were identified based on the next generation sequencing data of 121 cotton varieties. Among the 85 pairs of InDel primers synthesized, 64 were selected including 35 from At group and 29 from Dt group. The minimum average allelic frequency(MAF) of At and Dt chromosomes were 0.45 and 0.32, respectively, while the PIC were 0.49 and 0.40, respectively. The genetic distances of the 66 cotton varieties ranged from 0.04 to 0.65 centimorgan (cM), with an average of 0.39 cM. The two varieties with the largest genetic distance were Simian 3 and CCRI 36, and the two varieties with the smallest genetic distance were Xumian 18 and Xuza 3. [Conclusion] The 64 cotton dimorphic InDel markers can effectively reveal the relationships among varieties based on the genetic distance, and distinguish cotton varieties from different sources, which has certain theoretical significance and application value.     

优质黑猪育种30年工作体会

品种是影响养猪生产中的最重要因素,猪育种是一项复杂且系统的工程,文章从育种素材筛选、育种方案设计、育种方法选择、品种审定以及扩群产业化推广等方面进行详细的阐述,并结合育种经验提出相应猪育种建议。     

广州萝岗玉岩书院

玉岩书院坐落在广州市萝岗区萝峰山,是广州历史最早的书院之一。萝岗又名萝岗洞,洞即垌,盆地之意。自秦以来,是古代番禺县管辖的偏僻之地,古籍《萝峰纪略》说：＂萝峰,即萝岗洞之最深处。＂自明代后,萝峰山一带,已成为文人墨客赏梅胜地,萝岗自然条件独特,梅树常梅开二度,     

镁肥不同施用方法对水稻产量构成因素及产量影响

对镁肥不同施用方法进行试验研究,结果表明:合理施用镁肥,对防治水稻黄叶病、提高水稻产量有显著效果,可降低水稻黄叶率4.2%~15%,每公顷增产7.4%~10.8%。供试品种在镁肥施用量每公顷基施100 kg硫酸镁处理下产量最高,达到8 157.6 kg/hm2。