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旨在建立鸡血液样品中多种喹诺酮类药物残留的超高效液相色谱—串联质谱方法。样品经乙腈提取,经Waters Acquity UPLC BEH C18色谱柱分离,以甲醇和0.1%甲酸水溶液为流动相进行梯度洗脱,电喷雾正离子(ESI+)模式电离,MRM扫描模式检测。经方法学验证,该方法的线性关系、回收率、精密度均符合要求。应用超高效液相色谱—串联质谱法建立了鸡血液样品中多种喹诺酮类药物同时检测的方法,实现了动物血液样品中12种喹诺酮类药物同时定性、定量分析。 相似文献
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社会林业与五指山的自然保护 总被引:1,自引:0,他引:1
五指山自然保护区是世界上现存地势最高的原始热带雨林之一,是我省目前面积最大的自然保护区,也是海南三大河流的发源地。但由于保护区横跨琼中、通什、保亭三县、市,紧邻保护区还生存着众多贫困的黎、苗族村民。因此,保护区与周边社区的联系是十分紧密的,社区的发展也直接影响到五指山热带天然林的保护。海南省林业局中德海南热带林保护和恢复项目在社会林业与五指山森林的保护上做出了有益的尝试,就在全省来说,也是首次对社会林业与森林保护上进行的探索。五指山地区社会林业的研究,对保护海南热带雨林也有着积极的意义。特对社会林业与五指山的森林保护进行了阐述、分析和设想。 相似文献
25.
In a feeding trial lasting for months Halothane-positive fattening pigs were put on a diet containing different concentrations of vitamin E and selenium. After a Myostress application vitamin E and selenium were additionally injected in order to examine stress resistance (CK-test) and regenerative capacity (histological examination of Musculus longissimus dorsi) of skeletal muscle. The study revealed that neither high dietary levels of vitamin E/selenium nor a vitamin E/selenium treatment applied after stress are capable of preventing stress related myopathy nor can they influence its healing up favourably. 相似文献
26.
The aerobic bacterial flora of the genital tract was characterized in 15 stud dogs in an 18-month study. The dogs represented 4 breeds and were from 3 kennels. Bacterial samples from the prepuce and semen were collected every month, except in connection with matings, when they were collected weekly (464 samples). The dogs that were included all mated at least once during the study. The mean pregnancy rate, litter size, and pup mortality for the bitches with which they had mated were all within normal limits. The most frequent bacteria isolated from the prepuce and semen were Pasteurella multocida, beta-hemolytic streptococci, and Escherichia coli. There was a tendency for breeds to differ in frequency of the most common bacterial species. Bacterial culture yielded no aerobic growth in 14.2% of the preputial samples and 69.8% of the semen samples. Bacteria were transferred between dog and bitch at mating. In this study of healthy breeding dogs, neither the fertility of the dog nor that of the bitch was affected by the bacteria transferred. 相似文献
27.
R K?stlin 《Tier?rztliche Praxis》1988,16(4):351, 436-351, 437
28.
选用1026只1周龄罗斯蛋用雏鸡为试验动物,研究不同日粮Se水平对雏鸡组织Se含量的影响。结果表明雏鸡的Se缺乏或过量均可通过各组织Se含量的变化反映出来,但反映的敏感性及临界指示值因组织及发育期不同而异。日粮Se水平缺乏或过量时,各组织Se含量相应降低或增加。在本试验条件下得出罗斯蛋用雏鸡的Se需要量范围为0.145×10 ̄(-6)~0.495×10 ̄(-6),其中以0.195×10 ̄(-6)最佳;日粮Se含量≤0.095×10 ̄(-6)或≥0.995×10 ̄(-6),雏鸡出现Se缺乏症或中毒症。 相似文献
29.
Eradication of lice in cattle. 总被引:8,自引:0,他引:8
The purpose of this field study was to develop and evaluate eradication as a strategy to control lice in cattle. Thirty-three herds of cattle were selected and observed during a period of two and a half years. Before eradication, biting lice (Damalinia bovis) were present in 94% of the herds and 27% of the animals. Sucking lice (Linognathus vituli) were present in 42% of the herds and 5% of the animals. These levels were very similar to those reported from other countries in Northern Europe. The eradication strategy was successful in 28 of 33 herds, but lice were still present in 5 herds 3 to 6 months after treatment. Biting lice were present in all these 5 herds, sucking lice were present in 3 herds. During the next 12 months, nine of the 28 herds were reinfected with lice. Six herds were reinfected with just biting lice, 2 herds with just sucking lice and one herd was reinfected with both. There was no significant difference between the 2 louse species regarding the risk of unsuccessful eradication or reinfection. The only significant risk factor for reinfection was either purchase of livestock or use of common pasture, combined with failure in pre-treatment of newly introduced animals. 相似文献
30.
M D Pérez Alenza E Tabanera L Pe?a 《Journal of the American Veterinary Medical Association》2001,219(8):1110-1114
OBJECTIVE: To determine epidemiologic, clinical, and pathologic characteristics of inflammatory mammary carcinoma (IC) in dogs. DESIGN: Retrospective study. ANIMALS: 33 dogs with IC and 153 dogs with malignant mammary tumors other than IC. PROCEDURES: Medical records were reviewed, and signalment, history, physical examination findings, and results of thoracic radiography and necropsy were obtained. RESULTS: 33 of 436 (7.6%) dogs examined at a veterinary teaching hospital because of dysplasia or tumors of the mammary glands and 33 of 186 (17.7%) dogs with at least 1 malignant tumor had IC. Thirty-two of the 33 dogs were sexually intact. Dogs with IC were significantly older than were dogs with other malignant mammary tumors, and in dogs with IC, the tumor was initially noticed a mean of 52 days after the beginning of the last observed estrus, whereas in dogs with other mammary tumors, the tumor was initially noticed a mean of 137 days after the beginning of the last observed estrus. Dogs with IC were more likely to be anorectic and to have generalized weakness, weight loss, and thoracic metastases. Dogs with IC survived a mean of 25 days with palliative treatment. Histologically, involvement of dermal lymphatic vessels was identified in 14 of 19 (74%) dogs with IC. Two clinical forms of IC (primary and secondary) were identified. Dogs with primary IC had a worse clinical condition. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that IC is an uncommon but distinct entity in dogs. A histologic finding of dermal lymphatic involvement should be considered a hallmark for the pathologic diagnosis of IC in dogs. 相似文献