配筋混凝土砌块高层设计时应注意的几个问题

针对配筋混凝土砌块高层设计,提出砌块空腔墙的应用,注意合理设置水平配筋,配筋砌块深梁,控制底框房屋层间抗侧刚度比及高层墙体的裂缝问题.     

Second round of an interlaboratory comparison of SARS-CoV2 molecular detection assays used by 45 veterinary diagnostic laboratories in the United States

The COVID-19 pandemic presents a continued public health challenge. Veterinary diagnostic laboratories in the United States use RT-rtPCR for animal testing, and many laboratories are certified for testing human samples; hence, ensuring that laboratories have sensitive and specific SARS-CoV2 testing methods is a critical component of the pandemic response. In 2020, the FDA Veterinary Laboratory Investigation and Response Network (Vet-LIRN) led an interlaboratory comparison (ILC1) to help laboratories evaluate their existing RT-rtPCR methods for detecting SARS-CoV2. All participating laboratories were able to detect the viral RNA spiked in buffer and PrimeStore molecular transport medium (MTM). With ILC2, Vet-LIRN extended ILC1 by evaluating analytical sensitivity and specificity of the methods used by participating laboratories to detect 3 SARS-CoV2 variants (B.1; B.1.1.7 [Alpha]; B.1.351 [Beta]) at various copy levels. We analyzed 57 sets of results from 45 laboratories qualitatively and quantitatively according to the principles of ISO 16140-2:2016. More than 95% of analysts detected the SARS-CoV2 RNA in MTM at ≥500 copies for all 3 variants. In addition, for nucleocapsid markers N1 and N2, 81% and 92% of the analysts detected ≤20 copies in the assays, respectively. The analytical specificity of the evaluated methods was >99%. Participating laboratories were able to assess their current method performance, identify possible limitations, and recognize method strengths as part of a continuous learning environment to support the critical need for the reliable diagnosis of COVID-19 in potentially infected animals and humans.     

基于SRAP分子标记的11份柑橘种质材料遗传多样性分析

为分析11份柑橘种质材料的遗传多样性,本研究以7份沙糖橘种质以及4份其他柑橘种质为试验材料,利用SRAP分子标记进行分析,并用UPGMA法进行聚类.结果表明:160对引物组合筛选出20对引物组合扩增条带清晰,11份种质材料两两之间遗传相似系数在0.322 1～0.931 6之间.沙糖橘和金葵蜜橘间的遗传相似系数最大,为...     

全球气候变化对松嫩草原土壤水分和生产力影响的研究

本文首先模拟计算松嫩草原土壤水量平衡,并结合未来气候情景估算松嫩草原土壤水分的变化。最后,在土壤水分研究的基础上,初步评估全球气候变化对松嫩草原生产力的影响。     

我国执业兽医资格考试现状

我国执业兽医准入制度已经建立,但执业兽医体系建设才刚刚开始。通过对全国执业兽医资格考试情况的分析,建议从三个方面加快建设：一是通过立法保障执业兽医社会职责的发挥及应有社会地位的获得;二是提高我国执业兽医的本科教育及继续教育水平,建立教育机构评估机制;三是完善执业兽医配套岗位和工作制度,通过行业协会更好地规范执业行为,加快与国际接轨的步伐。     

中国矮马与普氏野马的亲缘关系

产于中国西南山地的矮马是一种珍贵的资源,它的优良品质可以与原产于设得兰群岛的欧洲矮马媲美。本文研究了产自广西德保的中国矮马骨骼标本,通过与欧洲矮马的对比,发现它们之间的头骨、颊齿和肢骨上都 存在较大的差异,显然亲缘关系较远。而中国矮马与普氏野马的头骨的比例上却相当一致,头骨在马属的系统演化中具有非常重要的意义,因此,这一结果证明中国矮马与普氏野马有密切的亲缘关系。尽管从汉代起中国已广泛引入西方马种     

高效液相色谱法测定猪血浆及组织中喹赛多及其脱二氧代谢物

建立了测定猪血浆及肝脏、肾脏、肌肉等组织中的喹赛多及其代谢物脱二氧喹赛多的高效液相色谱(HPLC)法.血浆样用甲醇沉淀蛋白后离心取上清液进样测定.组织样先用乙腈匀浆,用正己烷脱脂后作HPLC检测.色谱柱为ODS C18柱;流动相血浆样测定为乙腈水(2080),组织样测定为甲醇水(4258),流速为1.0 mL/min;紫外检测波长305 nm.药物工作液质量浓度范围为0.005～0.500 mg/L时,血浆样品及组织样品测定条件下药物质量浓度与响应值均具良好线性关系,相关系数＞0.999.血浆中药物质量浓度为0.02、0.10、0.50 mg/L时,喹赛多及脱二氧喹赛多的回收率均大于70%,组织中药物含量为0.05、0.20、1.00μg/g时,肌肉样品的回收率均大于70%,肝脏、肾脏样品则为50%～80%.本试验条件下,喹赛多及其脱二氧代谢物的最低检出质量浓度,血浆样品分别为0.01、0.02mg/L,组织样品2种检测物均为0.025μg/g.测定了工作液3种质量浓度0.01、0.05、0.25 mg/L的仪器精密度,日内相对偏差＜8.0%,日间相对偏差＜17.0%.     

Mutant-prevention concentrations of enrofloxacin for Escherichia coli isolates from chickens

OBJECTIVE: To investigate the development of enrofloxacin resistance among Escherichia coli isolates obtained from chickens by determining mutant-prevention concentrations (MPCs) and sequence the quinolone resistance-determining regions (QRDRs) of gyrA and parC genes in selected isolates. SAMPLE POPULATION: 15 chicken-derived E coli isolates. PROCEDURES: For all isolates, MPC and minimal inhibition concentration (MIC) of enrofloxacin were determined. The MPCs and maximum serum drug concentrations attained with enrofloxacin doses recommended for treatment of E coli infections in chickens were compared. Mutation frequencies and QRDR sequence changes in gyrA and parC were also determined. RESULTS: In 2 of 15 E coli strains, MPCs were low (0.016 and 0.062 microg/mL), MPC:MIC ratios were 2 and 4, and the GyrA and ParC proteins had no mutations. In 9 susceptible isolates with a GyrA point mutation, MPCs ranged from 2 to 16 microg/mL. For isolates with double mutations in GyrA and a single mutation in ParC, MPCs were > 32 microg/mL (several fold greater than the maximal plasma concentration of enrofloxacin in chickens); mutation frequencies were also much lower, compared with frequencies for single-mutation isolates. CONCLUSIONS AND CLINICAL RELEVANCE: For E coli infections of chickens, MPC appears to be useful for determining enrofloxacin-dosing strategies. The high MPC:MIC ratio may result in enrofloxacin-treatment failure in chickens infected with some wild-type gyrA E coli isolates despite the isolates' enrofloxacin susceptibility (MICs 0.125 to 1 microg/mL). For infections involving isolates with high MPCs, especially those containing mutations in gyrA and parC genes, treatment with combinations of antimicrobials should be adopted.     

对奶牛胎衣不下的认识和处理

本文结合本场的生产实际，讨论了母牛胎盘的特点，胎衣不下的症状与诊断；着重讨论了本病的发病率及其影响因素，并将病因分析、归纳成一个示意图；最后综述了多项治疗和预防胎衣不下的措施，如灌服羊水，注射激素，用维生素A、D、E和亚硒酸钠，灌服中药，手术剥离以及电刺激等。