Timing and propane dose of broadcast flaming to control weed population influenced yield of sweet maize (Zea mays L. var. rugosa)

Farmers are interested to produce sweet maize under organic production systems and propane flaming could be a potential alternative tool for weed control in organic sweet maize production. Therefore, the objective of this study was to investigate the response of sweet maize to broadcast flaming as influenced by propane dose and crop growth stage. Field experiments were conducted at the Haskell Agricultural Laboratory of the University of Nebraska, Concord, NE in 2008 and 2009 using five propane doses applied at three different growth stages of V2 (2-leaf), V5 (5-leaf) and V7 (7-leaf). The propane doses were 0, 13, 24, 44 and 85 kg ha⁻¹. The response of sweet maize to propane flaming was evaluated in terms of visual crop injury, effects on plant height, yield components (plants m⁻², tillers plant⁻¹, number of ears plant⁻¹, cob length and number of seeds cob⁻¹) and fresh marketable yield. The response of different growth stages of sweet maize to propane doses was described by log-logistic models. Based on most parameters tested, V7 was the most tolerant while V2 was the least tolerant stage for broadcast flaming. The maximum yield reductions with the highest propane dose of 85 kg ha⁻¹ were 22%, 12% and 6% for V2, V5 and V7 stages, respectively. Furthermore, a 5% yield reduction was evident with 23, 25 and 36 kg ha⁻¹ of propane for V2, V5 and V7 growth stages, respectively, suggesting that plants flamed at V7 stage can tolerate higher dose of propane for the same yield reduction compared to the other growth stages. We believe that flaming has a potential to be used effectively in organic sweet maize production if properly used.     

Impact of different combined doses of fertilizers with plant growth regulators on growth, yield attributes and yield of mustard (Brassica campestris cv. B9) under old alluvial soil of Burdwan, West Bengal, India

Field experiments were conducted with mustard (Brassica campestris cv. B₉) consecutively for three years (2005, 2006 and 2007) in the Crop Research and Seed Multiplication Farm of Burdwan University, Burdwan, West Bengal, India. In the first year, varietal screening of mustard under recommended dose of chemical fertilizer (100:50:50) were performed with seven mustard varieties during November 2005 to February 2006. In the second year, two experiments were conducted to study the effects of six different combined doses of chemical fertilizer and biofertilizer and six different levels of growth retardant cycocel. In the third year, six different levels of compost along with the best screened dose of growth retardant (CCC-300 ppm) and the best combined dose of biofertilizer and chemical fertilizer (3/4thchemical fertilizer:1/4th biofertilizer) from the previous year were applied to study the effects on agronomic traits and growth attributes of mustard, and the field data were analyzed statistically. The variety B₉ recorded a significant higher seed yield along with other yield contributing factors, which was found to be superior to other varieties under old alluvial soil of Burdwan, West Bengal, India, in 2005. In 2006 and 2007, seed yield was found to be the best for CCC-300 ppm treatment and the best combined dose of biofertilizer and chemical fertilizer was found to be 3/4th chemical fertilizer:1/4th biofertilizer. In 2007, the best yield was given by the treatment of 7.5 t·hm^?2 compost along with the best dose of growth retardant cycocel and the best combined dose of biofertilizer and chemical fertilizer.     

Row spacing impacts the critical period for weed control in cotton (<Emphasis Type="Italic">Gossypium hirsutum</Emphasis>)

The knowledge on the critical crop-weed competition period is important for designing an efficient weed management program. Field studies were conducted in 2012 and 2013 at the Agricultural Research Institute, Kahramanmaras, Turkey to determine the effects of three row spacing (50, 70 and 90 cm) on the critical period for weed control (CPWC) in cotton. A four parameter logistic equation was fit to data relating relative crop yield to both increasing duration of weed interference and length of weed-free period. The relative yield of cotton was influenced by the duration of weed-infested or weed-free period, regardless of row spacing. In cotton grown at 50 cm row spacing, the CPWC ranged from 117–526 growing degree days (GDD) (V2–V11 growth stages) in 2012 and 124–508 GDD (V2–V10) in 2013 based on the 5% acceptable yield loss level. At 70 cm row spacing, the CPWC ranged from 98–661 GDD in 2012 (V2–V13) and 144–616 GDD (V2–V12) in 2013. At 90 cm row spacing, the CPWC ranged from 80–771 GDD in 2012 (V1–V14) and 83–755 GDD (V1–V14) in 2013. In order to obtain a 95% weed-free yield, the weed management should start at 16 days after crop emergence (DAE) and continued until 52 DAE (V2–V11) for crops grown in 50 cm row spacing, 15 and 60 DAE (V2–V13) for 70 cm row spacing and 11 and 67 DAE (V1–V14) for crops grown in 90 cm row spacing. This suggests that cotton grown in narrow row spacing (50 cm) had greater competiveness against weeds compared with wider row spacing (70 and 90 cm). Cotton growers can benefit from these results by improving cost of weed control through better timing of weed management.     

Identification of Stable Reference Genes for Gene Expression Studies Using Quantitative Real Time PCR in Buffalo Oocytes and Embryos

The present study was aimed to validate expression stability of 6 housekeeping genes (viz. YWHAZ, SDHA, GAPDH, RPS15, RPS18 and RN18S1) in the oocytes and embryos of different stages in buffalo. A modified Trizol protocol was optimized for RNA isolation from as few as five oocytes. The expression level of selected genes was studied by an optimized real time PCR using DCT method and their stability of expression was evaluated by Microsoft Excel based visual application, geNORM. The analysis revealed that the RPS15 and GAPDH were the most stable genes across different samples. Also, the geometric mean of three genes (i.e. RPS15, RPS18 and GAPDH) were found suitable for normalization of real time PCR data from buffalo oocytes/embryos. The information would help in more accurate interpretation of gene expression data from oocytes/embryos towards understanding the molecular events in these cells during development.     

Bt籼稻对褐飞虱取食、产卵行为的影响

比较了褐飞虱在2个Bt籼稻纯合品系，即TT9-3、TT9-4，及亲本对照IR72上的取食和产卵行为。结果表明，在选择性条件下，褐飞虱于稻株上的着虫比率在Bt籼稻与对照间无显著差异，而稻株中部和整个叶鞘的取食刺探痕数、产卵选择比率和产卵量则均以Bt籼稻显著为低。在非选择性条件下，稻株叶鞘上的取食刺探痕数在Bt籼稻与对照间无显著差异，而排泄的蜜露量则多以Bt籼稻尤其是TTP9-4显著为少。可见，供试的2个Bt籼稻品系不利于褐飞虱的取食或产卵，表现有抗褐飞虱的能力。     

Growth characteristics of multipurpose tree species,crop productivity and soil properties in agroforestry systems under subtropical humid climate in India

Multipurpose tree species （MPTs） were studied in an agroforestry arboretum under subtropical humid climate in Northeast India. Out of 12 MPTs planted under agroforestry systems, Acacia auriculiformis in spacing of 2 m × 2 m （2500 stems·hm^-2） could have the potentiality to meet the timber/fuelwood requirement due to its high wood production of 635 m^3·hm^-2 with mean annual increment （MAI） of 2.54×10^-2 m^3.treel.a^-1 in a short rotation period of 10 years. Thus, A. auriculiformis is a short rotation forest tree species suitable to grow in subtropical humid climate. On the other hand, at 16 years of age, Eucalyptus hybrid and Michelia champaca in spacing of 3 m × 3 m （1111 stems.hm^2） produced appreciably high timber volume of 315 m^3.hm^-2 and 165 m^3.hm^-2 with MAI of 1.77×10^-2 m^3.tree^-1·a^-1 and 0.92×10.2 m^3.tree^-1.a^-1, respectively. At 16 years of age, Gmelina arborea produced a timber volume of 147 m^3.hm^-2 with MAI of 1.47×10^-2 m^3.tree^-1.a^-1 followed by Samania saman （140 m^3.hm^-2）, Albizziaprocera （113 m^3·hm^-2） and Tectona grandis （79 m3.hm^-2） with MAI of 1.40, 1.13 and 0.78 × 10^-2 m^3 .tree^-1a^-1, respectively in 4 m × 4 m spacing （625 stems.hm^-2）. Gliricidia maculata and Leucaena leucocephala could be used as live fences around the farm boundary to supply their N-rich leaves for mulch as well as manure to crops. In agroforestry arboretum, direct seeded upland rice （Oryza sativa - variety, AR-11）, groundnut （Arachis hypogaea - variety, JL-24） and sesamum （Sesamum indicum - variety, B-67） were grown during the initial period upto 8 years of tree establishment. Under other MPTs, there was a reduction in crop productivity as compared to open space. After 8 years of tree establishment, horti-silvi and silvi-pastoral systems were developed and pineapple （Ananas comosus - variety Queen）, turmeric （Curcuma longa -variety RCT -1） and cowpea （Vigna sinensis - variety Pusa Barsati） as forage crop were raised. The productivity of p     

Ethno-medicinal plants used by Bengali communities in Tripura,northeast India

Northeastern India has high medicinal plant diversity due to variance in topography and physiognomy. We documented the uses of various medicinal plants by the Bengali people of West district and South district of Tripura state for their own health care as well as for domesti-cated animals. Based on semi structured interviews, group discussions and information from local informants, a total of 93 species of medicinal plants of 52 families and 83 genera were documented. These plants were used to treat more than 55 different human diseases and 6 diseases of livestock. Sixty-eight plant species were used singly and the rest were used in combination with other species for therapeutic formulations of various diseases. Leaves of plants were most often used for most of the ethnobotanical preparations. Maximum consensus value of 96% was recorded for Chromolaena odorata （L.） King ＆amp;H. Rob., and the mini-mum was 15%for Bambusa balcooa Robx. Of the 93 plant species, 75 species showed pharmacological properties. Prospects for augmenting existing knowledge and enhancing the use of traditional medicinal plants are discussed.