Fusarium crown rot under continuous cropping of susceptible and partially resistant wheat in microcosms at elevated CO2

This study examines the CO₂‐mediated influence of plant resistance on crown rot dynamics under continuous cropping of partially resistant wheat line 249 and the susceptible cultivar Tamaroi. Disease incidence, severity, deoxynivalenol and Fusarium biomass were assessed after each cycle in microcosms established at ambient and 700 mg kg^?1 CO₂ using soil and stubble of these wheat lines from a field experiment with free to air CO₂ enrichment. Monoconidial isolates from wheat stubble were collected initially, and after five cropping cycles, to compare the frequency and aggressiveness of Fusarium species in the two populations. Aggressiveness was measured using a high‐throughput seedling bioassay. At elevated CO₂, the higher initial incidence in Tamaroi increased with cropping cycles, but incidence in 249 remained unchanged. Incidence at ambient CO₂ did not change for either line. Elevated CO₂ induced partial resistance in Tamaroi, but not in 249. Increased Fusarium biomass in wheat tissue at elevated CO₂ matched raised deoxynivalenol of the stem base in both lines. After five cycles of continuous wheat cropping, aggressiveness increased in pathogenic F. culmorum and F. pseudograminearum by 110%, but decreased in weakly pathogenic F. equiseti and F. oxysporum by 50%. CO₂ and host resistance interactively influenced species frequency, and the highly aggressive F. pseudograminearum became dominant on Tamaroi irrespective of CO₂ concentration, while its frequency declined on 249. This study shows that induced resistance at elevated CO₂ will not reduce crown rot severity, or impede the selection and enrichment of Fusarium populations with increased aggressiveness.     

Comparative proteomic analysis of high‐ and low‐fertile buffalo bull spermatozoa for identification of fertility‐associated proteins

The present study identified few potential proteins in the spermatozoa of buffalo bulls that can be used as an aid in fertility determination through comparative proteomics. The sperm proteome of high‐fertile buffalo bulls was compared with that of low‐fertile buffalo bulls using two‐dimensional difference gel electrophoresis (2D‐DIGE), and the differentially expressed proteins were identified through mass spectrometric method. The protein interaction network and the functional bioinformatics analysis of differentially expressed proteins were also carried out. In the spermatozoa of high‐fertile bulls, 10 proteins were found overexpressed and 15 proteins were underexpressed at the level of twofold or more (p ≤ 0.05). The proteins overexpressed in high‐fertile spermatozoa were PDZD8, GTF2F2, ZNF397, KIZ, LOH12CR1, ACRBP, PRSS37, CYP11B2, F13A1 and SPO11, whereas those overexpressed in low‐fertile spermatozoa were MT1A, ATP5F1, CS, TCRB, PRODH2, HARS, IDH3A, SRPK3, Uncharacterized protein C9orf9 homolog isoform X4, TUBB2B, GPR4, PMP2, CTSL1, TPPP2 and EGFL6. The differential expression ranged from 2.0‐ to 6.1‐fold between the two groups, where CYP11B2 was high abundant in high‐fertile spermatozoa and MT1A was highly abundant in low‐fertile spermatozoa. Most of the proteins overexpressed in low‐fertile spermatozoa were related to energy metabolism and capacitation factors, pointing out the possible role of pre‐mature capacitation and cryo‐damages in reducing the fertility of cryopreserved buffalo spermatozoa.     

Quercetin 3-O-(6"-caffeoyl)-beta-D-galactopyranoside from Polygonum viscosum

The methanol extract of the whole plant parts of Polygonum viscosum has yielded a flavonol glycoside, 3-O-(6"-caffeoyl)-beta-D-galactopyranoside (1), the structure of which has been determined unambiguously by UV and a series of one- and two-dimensional NMR experiments, notably, (1)H, (13)C, DEPT, COSY45, HMBC and HMQC.     

Genetic analysis of adult plant leaf rust resistance in three bread wheat (Triticum aestivum L.) cultivars

Genetic basis of adult plant leaf rust resistance in three released Indian wheat cultivars viz. DWR195, RAJ3765 and HP1731 was investigated through detailed inheritance study under controlled polythene house condition at Flowerdale, India. The F₂, F_3, F₄ and F₅ generations were analyzed with the most frequent and virulent Indian leaf rust pathotype 121R63-1. Two complementary recessive genes imparted resistance in DWR195, two complementary dominant genes governed the resistance of RAJ3765 whereas two independent dominant genes were involved in the resistance of HP1731. The genes responsible for adult plant resistance in the three cultivars were not allelic. The two complementary genes of DWR195 and two independent dominant genes of HP1731 have been isolated as single gene lines. Utilization of resistance from HP1731, which carries two independent dominant genes, will be easy as compared to DWR195 and RAJ3765.     

Effect of aggregate size on water uptake by peanut seeds

In a lateritic sandy clay loam soil contained in a soil bin, three aggregate size ranges (0.25–0.50, 1.0–2.0 and 2.0–4.0 mm) were established by different tillage intensity to vary the seed—soil contact and to determine the best seedbed for peanut seedling emergence. The general objective was to study the water uptake and diffusivity of the seeds as well as the aeration, water transmission and thermal properties of the seedbed. In the seedbed with the finest aggregate size, the water uptake was maximum and the time needed for germination was least.     

Interspecific hybridization between Corchorus sidoides F. Muell. and C. siliquosus L., a natural polyploid

In Corchorus sidoides F. Muell x C. siliquosus L. , the pod set was 19.05 per cent. and the percentage of pod harvested (out of set pods) was 100 per cent.. All seeds of the cross pods were non-viable.In C. siliquosus L. x C. sidoides F. Muell. , the pod set was 8.7 per cent. and the percentage of pod harvested was 100. In this case the seeds were also non-viable.The genus Corchorus comprises species which are systematically widely related. The possibility of getting viable hybrids in this case is impossible because the phylogenetic relationship does not allow of getting viable seeds. A karyotypic study of these species will throw light on this aspect and breeders will then get a tool for handling this subject.Department of Agriculture, Calcutta University     

EST‐SSR analysis provides insights about genetic relatedness,population structure and gene flow in grass pea (Lathyrus sativus)

Grass pea (Lathyrus sativus) is an important food‐legume crop for resource‐poor farmers in the developing world. However, given its cultivation in the most underprivileged regions, the crop has not received appropriate scientific attention particularly from the genomic perspective, thereby giving it a status of genomic orphan. Nevertheless, some recent studies have attempted to develop modern molecular tools to strengthen the genetic and genomic research. In the present investigation, a comprehensive collection comprising 176 accessions was analysed using EST‐simple sequence repeats (SSRs). The SSR analysis revealed existence of a total of 51 alleles with an average polymorphic information content value of 0.35. A moderate level of gene diversity was noticed that ranged from 0.04 to 0.73 with an average of 0.43. Noticeably, two distinct subpopulations were recovered using cluster analysis. In addition, the presence of admixtures in population reflected the strong possibilities of gene flow between the accessions across the geographical boundary. In summary, we provide additional insights about the informativeness of available EST‐SSR markers along with an extended understanding of relatedness, genetic structure and gene flow in an under‐researched legume crop.     

Maize response to broadcast flaming at different growth stages: Effects on growth,yield and yield components

Weed management is a major constraint in organic crop production. Propane flaming could be an additional tool for weed control in organic maize (Zea mays L.) production. However, tolerance of maize to broadcast flaming must be determined first to optimize the use of propane. Field experiments were conducted at the Haskell Agricultural Laboratory of the University of Nebraska, Concord, NE in 2008 and 2009 to determine maize response to five propane doses applied at three growth stages of V2 (2-leaf), V5 (5-leaf) and V7 (7-leaf). The propane doses tested were 0, 13, 24, 44 and 85 kg ha^?1. Flaming treatments were applied utilizing a custom built research flamer mounted on the back of a four-wheeler moving at a constant speed of 6.4 km h^?1. The response of maize to propane flaming was evaluated in terms of visual crop injury (1, 7, 14 and 28 days after treatment—DAT), effects on dry matter (14 DAT), yield components (plants m^?2, ears plant^?1, kernels cob^?1 and 1000-kernel weight) and grain yield. The response of different growth stages of maize to propane doses was described by log–logistic models. Overall, maize response to broadcast flaming varied among growth stages and propane doses. In general, maize at V5 was the most tolerant stage for broadcast flaming, whereas V2 stage was the most susceptible to flaming resulting in the highest visual crop injury ratings, dry matter reductions and the largest loss of yield and its components. At 28 DAT, the maximum visual crop injury rating of 7% was estimated for flaming done at V5 stage compared to significantly higher injuries of 43% and 12% for V2 and V7 growth stages, respectively. An arbitrarily assigned 2.5% yield reduction was evident with 33, 16 and 11 kg ha^?1 of propane for V5, V2 and V7 growth stages, respectively, suggesting that maize flamed at V5 stage can tolerate higher dose of propane for the same yield reduction compared to other growth stages. Moreover, the maximum yield reductions with the highest propane dose of 85 kg ha^?1 were 3% for V5, 11% for V7 and 17% for V2 stage. Based on these results, flaming has a potential to be used effectively in organic maize production when conducted properly at V5 stage. However, there are both benefits and concerns associated with the use of flame weeding. The preservation of the soil from erosion and the protection of the surface and underground water from chemical pollution can be seen as benefits, while the concerns include higher energy use, and the release of greenhouse gasses.