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21.
A. C. Dario J. M. Salgado 《Plant foods for human nutrition (Dordrecht, Netherlands)》1994,46(3):213-219
The quality of the cowpea bean protein was improved through supplementations with flours from beans exposed to microwave oven treated with cereal proteins such as wheat, rice, corn, and sorghum. Biological assays results with these blends showed that the casein exceeded the other diets concerning digestibility only; however, in parameters such as biological value, net protein utilization (NPU), protein efficiency ratio (PER) and nutritional efficiency ratio (NER), no significant differences occurred. Among all elaborated blends, the one with irradiated beans submitted to microwave oven for 30 minutes (65%) + rice (35%) presented the best results. The soup elaborated with the best supplemented blend was satisfactory concerning color, odor, flavor and texture. 相似文献
22.
Castoria R Mannina L Durán-Patrón R Maffei F Sobolev AP De Felice DV Pinedo-Rivilla C Ritieni A Ferracane R Wright SA 《Journal of agricultural and food chemistry》2011,59(21):11571-11578
The infection of stored apples by the fungus Penicillium expansum causes the contamination of fruits and fruit-derived products with the mycotoxin patulin, which is a major issue in food safety. Fungal attack can be prevented by beneficial microorganisms, so-called biocontrol agents. Previous time-course thin layer chromatography analyses showed that the aerobic incubation of patulin with the biocontrol yeast Rhodosporidium kratochvilovae strain LS11 leads to the disappearance of the mycotoxin spot and the parallel emergence of two new spots, one of which disappears over time. In this work, we analyzed the biodegradation of patulin effected by LS11 through HPLC. The more stable of the two compounds was purified and characterized by nuclear magnetic resonance as desoxypatulinic acid, whose formation was also quantitated in patulin degradation experiments. After R. kratochvilovae LS11 had been incubated in the presence of (13)C-labeled patulin, label was traced to desoxypatulinic acid, thus proving that this compound derives from the metabolization of patulin by the yeast. Desoxypatulinic acid was much less toxic than patulin to human lymphocytes and, in contrast to patulin, did not react in vitro with the thiol-bearing tripeptide glutathione. The lower toxicity of desoxypatulinic acid is proposed to be a consequence of the hydrolysis of the lactone ring and the loss of functional groups that react with thiol groups. The formation of desoxypatulinic acid from patulin represents a novel biodegradation pathway that is also a detoxification process. 相似文献
23.
Carolina Fernandez Dario Mascolo Sean J. Monaghan Johanna L. Baily Lynn Chalmers Giuseppe Paladini Alexandra Adams James E. Bron Sophie Fridman 《Journal of fish diseases》2019,42(6):883-894
Two aqueous fixation methods (modified Davidson's solution and modified Davidson's solution with 2% (w/v) Alcian blue) were compared against two non‐aqueous fixation methods (methacarn solution and methacarn solution with 2% (w/v) Alcian blue) along with the standard buffered formalin fixation method to (a) improve preservation of the mucous coat on Atlantic salmon, Salmo salar L., gills and (b) to examine the interaction between the amoebae and mucus on the gill during an infection with amoebic gill disease. Aqueous fixatives demonstrated excellent cytological preservation but failed to deliver the preservation of the mucus when compared to the non‐aqueous‐based fixatives; qualitative and semi‐quantitative analysis revealed a greater preservation of the gill mucus using the non‐aqueous methacarn solution. A combination of this fixation method and an Alcian blue/Periodic acid–Schiff staining was tested in gills of Atlantic salmon infected with amoebic gill disease; lectin labelling was also used to confirm the mucus preservation in the methacarn‐fixed tissue. Amoebae were observed closely associated with the mucus demonstrating that the techniques employed for preservation of the mucous coat can indeed avoid the loss of potential mucus‐embedded parasites, thus providing a better understanding of the relationship between the mucus and parasite. 相似文献
24.
25.
Anniballi F Auricchio B Delibato E Antonacci M De Medici D Fenicia L 《Veterinary microbiology》2012,154(3-4):332-338
Clostridium botulinum type C and type D belonging to the group III organisms, are mainly responsible for animal botulism outbreaks. Clinical signs alone are often insufficient to make a diagnosis of botulism and a laboratory confirmation is required. Laboratory confirmation can be performed by demonstrating the presence of botulinum neurotoxins in serum, gastrointestinal contents, liver, wound of sick or dead animals, or by demonstrating the presence of C. botulinum in gastrointestinal contents, liver, and wound. Demonstration of spores in gastrointestinal contents or tissue of animals with clinical signs indicative of botulism reinforces the clinical diagnosis. With the aim of detecting and typing C. botulinum group III organisms, a multiplex real-time PCR SYBR Green was developed and in-house validated. Selectivity, limit of detection, relative accuracy, relative specificity, relative sensitivity, and repeatability of the method were investigated. The multiplex real-time PCR SYBR green used showed a 100% selectivity, 100% relative accuracy, 100% relative specificity, 100% relative sensitivity and a limit of detection of 277 and 580 DNA copies for C. botulinum type C and C. botulinum type D, respectively. The method reported here represents a suitable tool for laboratory diagnosis of type C and D botulism and for testing a large number of samples collected during the animal botulism surveillance and prevention activities. 相似文献
26.
Powdery mildew fungi are parasitized by strains of the genetically distinct Ampelomyces quisqualis. To investigate whether differences in the phylogeny and other cultural, morphological and physiological characteristics
of these different strains are related to differences in their geographic origins or the host species from which they were
isolated, several A. quisqualis strains isolated from different species of Erysiphaceae collected in different countries and possessing different ITS rDNA
sequences were selected and characterized. The results revealed some significant variation among the selected strains, which
provides evidence for the existence of different physiological forms within the A. quisqualis species. Two groups that display differential growth on artificial media were identified. These groups also differ in the
morphology of their mycelium, but not in the morphology of their pycnidia and conidia. Temperature greatly affected the in vitro growth of the A. quisqualis strains and growth rate was closely correlated to colony color. Differences in the conidial germination of distinct strains
were observed during the recognition phase of the parasitic relationship. The germination of each of the investigated strains
was greatly stimulated by all of the examined powdery mildew species and not only by the conidia of their original hosts.
An Italian strain isolated from grapevine in the Trentino Alto-Adige region was identified as the strain that germinates the
most quickly in the presence of powdery mildew conidia. Phylogenetic analysis revealed that these A. quisqualis strains can be classified into five different genetic groups, which generally correlate with the fungal host of origin and
morphological and growth characteristics. 相似文献
27.
L. Zavattaro M. Romani D. Sacco M. Bassanino C. Grignani 《Paddy and Water Environment》2006,4(1):61-66
The fertilization management of the rice crop in Piedmont was analyzed at a regional scale, and the agronomic and environmental
sustainability of the actual fertilization strategy of rice was evaluated through the analysis of its effect on the soils
and waters quality.
On average, a total amount of 127 kg ha−1 of N, 67 kg ha−1 of P2O5 and 161 kg ha−1 of K2O were supplied to the rice crop.
In most cases N and P fertilization was rather well balanced with crop removal. The N balance was in the range ±50 kg for
77% of the surface. The low concentration of N in the groundwater reflected the small N surplus. P fertilization resulted
to be smaller than removal for 53% of the surface. Nevertheless, the soil extractable P was very high, probably because of
former higher P inputs. This resulted in a high concentration in water courses and aquifers. The K fertilization was excessive
(surplus >100 kg ha−1) for 53% of the surface, but most soils showed a low K content. K is probably contributing to nutrient leaching to a great
extent.
The average soil organic matter (SOM) content of paddy fields was higher than that of normally-cultivated soils in Piedmont,
and the C/N was higher, owing to the low mineralization rate in waterlogged conditions. The SOM content was in relation with
the management of the crop residues, as the tradition of burning straw after harvest was still widespread on 65% of the paddy
surface. 相似文献
28.
Sacco RE Waters WR Rudolph KM Drew ML 《Comparative immunology, microbiology and infectious diseases》2006,29(1):1-11
Bighorn sheep are more susceptible to respiratory infection by Mannheimia haemolytica than are domestic sheep. In response to bacterial challenge, macrophages produce a number of molecules that play key roles in the inflammatory response, including highly reactive nitrogen intermediates such as nitric oxide (NO). Supernatants from monocyte-derived macrophages cultured with M. haemolytica LPS were assayed for nitric oxide activity via measurement of the NO metabolite, nitrite. In response to LPS stimulation, bighorn sheep macrophages secreted significantly higher levels of NO compared to levels for non-stimulated macrophages. In contrast, levels of NO produced by domestic sheep macrophages in response to M. haemolytica LPS did not differ from levels detected in non-stimulated cell cultures. Nitrite levels detected in supernatants of LPS-stimulated bighorn macrophage cultures treated with an inducible nitric oxide synthase (INOS) inhibitor, N(G)-monomethyl-L-arginine, were similar to that observed in non-stimulated cultures indicating a role for the iNOS pathway. 相似文献
29.
Alexandre Alonso Alves Carla Cristina Gonçalves Rosado Danielle Assis Faria Lúcio Mauro da Silva Guimarães Douglas Lau Sérgio Hermínio Brommonschenkel Dario Grattapaglia Acelino Couto Alfenas 《Euphytica》2012,183(1):27-38
Eucalypts are susceptible to a wide range of diseases. One of the most important diseases that affect Eucalyptus plantations worldwide is caused by the rust fungus Puccinia psidii. Here, we provide evidence on the complex genetic control of rust resistance in Eucalyptus inter-specific hybrids, by analyzing a number of full-sib families that display different patterns of segregation for rust
resistance. These families are totally unrelated to those previously used in other inheritance studies of rust resistance.
By using a full genome scan with 114 genetic markers (microsatellites and expressed sequence tag derived microsatellites)
we also corroborated the existence and segregation of a resistance locus, explaining 11.5% of the phenotypic variation, on
linkage group 3, corresponding to Ppr1. This find represents an additional validation of this locus in totally unrelated pedigree. We have also detected significant
additive × additive digenic interactions with LOD >10.0 on several linkage groups. The additive and epistatic QTLs identified
explain between 29.8 and 44.8% of the phenotypic variability for rust resistance. The recognition that both additive and non-additive
genetic variation (epistasis) are important contributors to rust resistance in eucalypts reveals the complexity of this host-pathogen
interaction and helps explain the success that breeding has achieved by selecting rust-resistant clones, where all the additive
and non-additive effects are readily captured. The positioning of epistatic QTLs also provides starting points to look for
the underlying genes or genomic regions controlling this phenotype on the upcoming E. grandis genome sequence. 相似文献
30.
Dario D'Ovidio Giacomo Rossi Raffaele Melidone Francesca Menna Alessandro Fioretti 《Journal of Exotic Pet Medicine》2012,21(3):238-242
A 6-year-old spayed female ferret (Mustela putorius furo) exhibiting clinical signs of weakness, anorexia, lethargy, weight loss, and frequent urination was presented for a veterinary evaluation to determine the underlying cause of the aforementioned abnormal behavior and clinical condition. Physical examination revealed a large, firm, painless, movable subcutaneous mass at the base of the tail. Radiographic and ultrasound images confirmed the presence of a soft tissue mass compressing the sacrococcygeal vertebrae, but there was no evidence of metastatic lesions. Because of the poor prognosis and progressive deterioration of the animal's clinical condition, the ferret was humanely euthanized. Gross necropsy revealed a 4 × 4 × 3.5 cm, firm, yellow-tan, ovoid, subcutaneous mass wrapped around the rectum and the anus. The mass did not appear to breach the serosa. Evaluation of the abdominal cavity revealed a pale yellow liver, possibly associated with hepatic lipidosis with no gross evidence of metastasis in the body cavity. The histopathological features of the mass were consistent with a liposarcoma. To the best of the authors' knowledge, this is the first case of subcutaneous liposarcoma reported in a ferret. 相似文献