全文获取类型
收费全文 | 29056篇 |
免费 | 920篇 |
国内免费 | 5篇 |
专业分类
林业 | 4596篇 |
农学 | 1685篇 |
基础科学 | 211篇 |
5761篇 | |
综合类 | 1576篇 |
农作物 | 2627篇 |
水产渔业 | 3030篇 |
畜牧兽医 | 6307篇 |
园艺 | 1307篇 |
植物保护 | 2881篇 |
出版年
2023年 | 122篇 |
2022年 | 181篇 |
2021年 | 337篇 |
2020年 | 338篇 |
2019年 | 304篇 |
2018年 | 3138篇 |
2017年 | 3090篇 |
2016年 | 1713篇 |
2015年 | 519篇 |
2014年 | 567篇 |
2013年 | 837篇 |
2012年 | 1716篇 |
2011年 | 2959篇 |
2010年 | 2537篇 |
2009年 | 1654篇 |
2008年 | 2055篇 |
2007年 | 2332篇 |
2006年 | 697篇 |
2005年 | 736篇 |
2004年 | 654篇 |
2003年 | 681篇 |
2002年 | 473篇 |
2001年 | 251篇 |
2000年 | 277篇 |
1999年 | 226篇 |
1998年 | 65篇 |
1997年 | 57篇 |
1996年 | 49篇 |
1995年 | 36篇 |
1994年 | 35篇 |
1993年 | 48篇 |
1992年 | 99篇 |
1991年 | 64篇 |
1990年 | 71篇 |
1989年 | 76篇 |
1988年 | 76篇 |
1987年 | 59篇 |
1986年 | 71篇 |
1985年 | 61篇 |
1984年 | 60篇 |
1983年 | 47篇 |
1982年 | 33篇 |
1980年 | 32篇 |
1979年 | 52篇 |
1978年 | 50篇 |
1977年 | 54篇 |
1974年 | 46篇 |
1973年 | 54篇 |
1972年 | 47篇 |
1971年 | 43篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
31.
Gripshover EM Givens MD Ridpath JF Brock KV Whitley EM Sartin EA 《Veterinary microbiology》2007,125(1-2):11-21
Bovine viral diarrhea virus (BVDV) affects cattle populations causing clinical signs that range from subclinical immunosuppression to severe reproductive and respiratory problems. Detection and removal of persistently infected (PI) calves is the single most important factor for control and eradication of BVDV. Current testing strategies to detect PI calves rely heavily on immunohistochemistry (IHC) and a commercially available antigen capture ELISA (ACE) assay. These viral assays depend on 1 or 2 monoclonal antibodies which target the E(rns) glycoprotein of BVDV. The sensitivity and specificity of these two tests have been reported previously. The purpose of this research was to characterize a strain of BVDV (AU501) that was undetectable using IHC and ACE based on a single monoclonal antibody, but was consistently detected in samples from a Holstein steer using virus isolation and PCR testing. Sequencing of this AU501 viral isolate revealed a unique mutation in the portion of the genome coding for the E(rns) glycoprotein. This unique field strain of BVDV demonstrates the risk of relying on a single monoclonal antibody for detection of BVDV. Multiple testing strategies, including polyclonal or pooled monoclonal antibodies that detect more than one viral glycoprotein may be necessary to detect all PI calves and facilitate eradication of BVDV. 相似文献
32.
Diniz PP Maggi RG Schwartz DS Cadenas MB Bradley JM Hegarty B Breitschwerdt EB 《Veterinary research》2007,38(5):697-710
The purpose of this study was to determine the serological and molecular prevalence of Bartonella spp. infection in a sick dog population from Brazil. At the S?o Paulo State University Veterinary Teaching Hospital in Botucatu, 198 consecutive dogs with clinicopathological abnormalities consistent with tick-borne infections were sampled. Antibodies to Bartonella henselae and Bartonella vinsonii subsp. berkhoffii were detected in 2.0% (4/197) and 1.5% (3/197) of the dogs, respectively. Using 16S-23S rRNA intergenic transcribed spacer (ITS) primers, Bartonella DNA was amplified from only 1/198 blood samples. Bartonella seroreactive and/or PCR positive blood samples (n=8) were inoculated into a liquid pre-enrichment growth medium (BAPGM) and subsequently sub-inoculated onto BAPGM/blood-agar plates. PCR targeting the ITS region, pap31 and rpoB genes amplified B. henselae from the blood and/or isolates of the PCR positive dog (ITS: DQ346666; pap31 gene: DQ351240; rpoB: EF196806). B. henselae and B. vinsonii subsp. berkhoffii (pap31: DQ906160; rpoB: EF196805) co-infection was found in one of the B. vinsonii subsp. berkhoffii seroreactive dogs. We conclude that dogs in this study population were infrequently exposed to or infected with a Bartonella species. The B. henselae and B. vinsonii subsp. berkhoffii strains identified in this study are genetically similar to strains isolated from septicemic cats, dogs, coyotes and human beings from other parts of the world. To our knowledge, these isolates provide the first Brazilian DNA sequences from these Bartonella species and the first evidence of Bartonella co-infection in dogs. 相似文献
33.
34.
35.
36.
Anna Bassols José Angel Robles-Guirado Laura Arroyo Lourdes Soler Natalia García Raquel Pato Raquel Peña Yolanda Saco Ramon Armengol Fermín Lampreave María A. Alava Francesca Canalias Matilde Piñeiro 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2023,52(Z1):64-74
37.
Intercropping, drip irrigation, and the use of plastic mulch are important management practices, which can, when utilized simultaneously, increase crop production and save irrigation water. Investigating soil water dynamics in the root zone of the intercropping field under such conditions is essential in order to understand the combined effects of these practices and to promote their wider use. However, not much work has been done to investigate soil water dynamics in the root zone of drip-irrigated, strip intercropping fields under plastic mulch. Three field experiments with different irrigation treatments (high T1, moderate T2, and low T3) were conducted to evaluate soil water contents (SWC) at different locations, for different irrigation treatments, and with respect to dripper lines and plants (corn and tomatoes). Experimental data were then used to calibrate the HYDRUS (2D/3D) model. Comparison between experimental data and model simulations showed that HYDRUS (2D/3D) described different irrigation events and SWC in the root zone well, with average relative errors of 10.8, 9.5, and 11.6 % for irrigation treatments T1, T2, and T3, respectively, and with corresponding root mean square errors of 0.043, 0.035, and 0.040 cm3 cm?3, respectively. The results showed that the SWC in the shallow root zone (0–40 cm) was lower under non-mulched locations than under mulched locations, irrespective of the irrigation treatment, while no significant differences in the SWC were observed in the deeper root zone (40–100 cm). The SWC in the shallow root zone was significantly higher for the high irrigation treatment (T1) than for the low irrigation treatment, while, again, no differences were observed in the deeper root zone. Simulations of two-dimensional SWC distributions revealed that the low irrigation treatment (T3) produced serious severe water stress (with SWCs near the wilting point) in the 30–40 cm part of the root zone, and that using separate drip emitter lines for each crop is well suited for producing the optimal soil water distribution pattern in the root zone of the intercropping field. The results of this study can be very useful in designing an optimal irrigation plan for intercropped fields. 相似文献
38.
Nyland R. Falkenberg Giovanni Piccinni J. Tom Cothren Daniel I. Leskovar Charlie M. Rush 《Agricultural Water Management》2007
The applicability of commercially available remote sensing instrumentation was evaluated for site-specific management of abiotic and biotic stress on cotton (Gossypium hirsutum L.) grown under a center pivot low energy precision application (LEPA) irrigation system. This study was conducted in a field where three irrigation regimes (100%, 75%, and 50% ETc) were imposed on areas of Phymatotrichum (root rot) with the specific objectives to (1) examine commercial remote sensing instrumentation for locating areas showing biotic and abiotic stress symptomology in a cotton field, (2) compare data obtained from commercial aerial infrared photography to that collected by infrared transducers (IRTs) mounted on a center pivot, (3) evaluate canopy temperature changes between irrigation regimes and their relationship to lint yield with IRTs and/or IR photography, and (4) explore the use of deficit irrigation and the use of crop coefficients for irrigation scheduling. Pivot-mounted IRTs and an IR camera were able to differentiate water stress among irrigation regimes. The IR camera distinguished between biotic (root rot) and abiotic (drought) stress with the assistance of groundtruthing. The 50% ETc regime had significantly higher canopy temperatures than the other two regimes, which was reflected in significantly lower lint yields when compared to the 75% and 100% ETc regimes. Deficit irrigation down to 75% ETc had no impact on lint yield, indicating that water savings were possible without reducing yield. 相似文献
39.
Řebíčková Kristýna Bajer Tomáš Šilha David Ventura Karel Bajerová Petra 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(4):495-504
Plant Foods for Human Nutrition - The purpose of this study was to compare the yield, chemical composition, antimicrobial and antioxidant properties of essential oils isolated from leaves of Laurus... 相似文献
40.
Palavecino Pablo Martín Curti María Isabel Bustos Mariela Cecilia Penci María Cecilia Ribotta Pablo Daniel 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(3):326-336
Plant Foods for Human Nutrition - Sorghum is a major cereal crop with various agronomic advantages, contains health-promoting compounds and is gluten-free. There is a growing tendency to use... 相似文献