Dominant sources of dietary carbon and nitrogen for shrimp reared in extensive rice-shrimp ponds

Stable isotope analysis was used to determine the sources of dietary nitrogen (N) and carbon (C) for shrimp during the rearing phase in extensive rice‐shrimp ponds in My Xuyen and Gia Rai districts, Vietnam. Farm‐made feed was added as a food source in shrimp ponds in My Xuyen district, and based on stable isotope analyses, was generally a poor dietary source. The commercial formulated feed used in Gia Rai also appeared to contribute little directly to the nutritional needs of the shrimp. In contrast, the natural biota in all ponds appeared to contribute substantially. In particular, biota from beam trawls and benthic organic matter were the most likely sources of nutrition in My Xuyen ponds, while benthic organic matter was the main source in Gia Rai ponds. δ¹⁵N ratios in the natural biota in My Xuyen farms decreased over the growing season, suggesting increased N fixation in the case of the benthic organic matter reaching values as low as 1‰. This suggests N‐limitation in the ponds and that natural biota become increasingly dependent on N fixed by algae and/or other microorganisms. There is the potential to promote the growth of the plankton and hence, the other natural biota, by the judicious addition of fertilizer.     

热空气处理对红富士苹果贮藏品质及其生理指标的影响

采用38℃72h和96h热空气处理红富士苹果,经过16周0℃的冷藏期后,置于货架条件1周。试验结果表明,冷藏期间,与对照组（未经热处理）相比,处理组果实果皮叶绿素含量较低,呼吸强度和乙烯释放量保持较低水平;果实硬度较高,水溶性果胶低,而非水溶性果胶含量较高;可溶性蛋白和热稳定蛋白含量下降缓慢,热稳定蛋白含量在可溶性蛋白中所占比例较高。热空气处理可以较好地保持果肉细胞膜的稳定性,使果实货架期果面底色较黄,固酸比上升,果汁pH值较高,具有较好的外观品质。     

Fucose-Rich Sulfated Polysaccharides from Two Vietnamese Sea Cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera: Structures and Anticoagulant Activity

Fucosylated chondroitin sulfates (FCSs) FCS-BA and FCS-HS, as well as fucan sulfates (FSs) FS-BA-AT and FS-HS-AT were isolated from the sea cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera, respectively. Purification of the polysaccharides was carried out by anion-exchange chromatography on DEAE-Sephacel column. Structural characterization of polysaccharides was performed in terms of monosaccharide and sulfate content, as well as using a series of non-destructive NMR spectroscopic methods. Both FCSs were shown to contain a chondroitin core [→3)-β-d-GalNAc-(1→4)-β-d-GlcA-(1→]_n bearing sulfated fucosyl branches at O-3 of every GlcA residue in the chain. These fucosyl residues were different in pattern of sulfation: FCS-BA contained Fuc2S4S, Fuc3S4S and Fuc4S at a ratio of 1:8:2, while FCS-HS contained these residues at a ratio of 2:2:1. Polysaccharides differed also in content of GalNAc4S6S and GalNAc4S units, the ratios being 14:1 for FCS-BA and 4:1 for FCS-HS. Both FCSs demonstrated significant anticoagulant activity in clotting time assay and potentiated inhibition of thrombin, but not of factor Xa. FS-BA-AT was shown to be a regular linear polymer of 4-linked α-L-fucopyranose 3-sulfate, the structure being confirmed by NMR spectra of desulfated polysaccharide. In spite of considerable sulfate content, FS-BA-AT was practically devoid of anticoagulant activity. FS-HS-AT cannot be purified completely from contamination of some FCS. Its structure was tentatively represented as a mixture of chains identical with FS-BA-AT and other chains built up of randomly sulfated alternating 4- and 3-linked α-L-fucopyranose residues.     

油页岩有机碳矿化特征及在栽培基质中应用可行性分析

探讨油页岩有机碳矿化分解过程、有机碳组分变化特征以及环境因素作用规律可为评价油页岩在栽培基质中应用的可行性提供科学依据.试验在室内控制温度和水分条件下,分析了洗盐和未洗盐油页岩有机碳矿化动态变化特征.结果表明:油页岩基质在60 d培养期间,温度升高10℃使未洗盐基质总矿化量分别增加2%～28%(100%田间持水量)、2%～22%(80%田间持水量)和1%～15%(60%田间持水量);洗盐基质则分别增加2%～17%(100%田间持水量)、1%～5%(80%田间持水量)和7%～14%(60%田间持水量).将第60 d基质中活性有机碳含量进行回归分析,发现两种不同供试油页岩活性有机碳含量与温度和水分之间均呈正相关关系;未洗盐油页岩活性有机碳含量与温度和水分间相关性不显著,而洗盐后油页岩活性有机碳含量与温度和水分之间相关性显著(P=0.0214).用一级动力学方程拟合油页岩基质有机碳矿化动态得到未洗盐基质分解速率常数最大达1.2×10-3/d,洗盐基质其分解速率常数介于0.5×10-3～0.7×10-3/d.油页岩在长达60 d的培养过程中表现为有机碳持续分解、活性有机碳递增,证实其在基质栽培中的应用将对养分持续供给和维护作物根系生理活性发挥重要作用.     

黄土坡地径流收集膜下自流出滴灌溉系统设计及其生态效益研究

黄土坡地径流收集及膜下自流出滴灌溉技术可有效地收集丰雨期地表径流,就地储存,旱季利用自然高差灌入集水袋,经自流管进行膜下自流灌溉,可有效地提高该区降水利用率,有效地解决黄土高原地形破碎、渠系难以浇灌的难题.造林试验表明,该技术可明显地提高20～70 cm土层含水量,可使刺槐、油松、枣树造林成活率分达到97.7%、91.4%、90.5%,比对照分别提高了57.0%、77.8%和69.4%;结合幼树根基覆膜技术,在栽植第2年即可使刺槐、油松、枣树之主径分别比对照提高26.9%、55.2%和49.6%,树冠投影面积分别比对照提高90.9%、74.2%和51.8%.同时可明显地降低坡地土壤侵蚀模数,在降雨387 mm的情况下,处理区径流发生量及土壤侵蚀模数分别较对照下降38.3%和102.7%.具有良好的生态效益和社会效益,在立地条件差、地形破碎的黄土丘陵沟壑区是一项投资少、效益高、简单易行的抗旱新技术,在我国西北同类型干旱地区有着较好的推广前景.     

基于机器视觉的半干枣病害和裂纹识别研究

研究提出了一种基于机器视觉的病害和裂纹的识别方法。在H分量图中,依据半干枣在病害和非病害区域色调值差异提取病害区域,以提取的病害区域与枣表面积的比作为阈值确定较高的病害面积识别精度,可正确识别的感兴趣病害面积为16.87mm2,占枣投影面积的3.3%。为进一步提高在该病害面积识别精度的正确率,依据已确定的病害面积比阈值,将病害面积比值二值化,结合红枣区域颜色特征值H的均值和均方差,用SVM方法建立枣病害的识别模型,训练集和测试集的识别正确率分别为9 5.7 7%和9 5.7 9%。在I分量图中,对红枣区域进行Otsu’s阈值分割、图像局部属性统计和形态学处理,提取裂纹二值图像,依据裂纹图像不变距方法建立裂纹识别模型,训练集和测试集的识别正确率分别为94.90%和94.55%。     

应用热扩散技术对柠条锦鸡儿主根液流速率的研究

运用热扩散技术对柠条锦鸡儿根部液流速率(Fs)进行连续测定,同步进行环境要素的实时监测。选择2008年4—10月30天典型晴天日观测数据。结果表明:1)Fs在晴天日表现出典型的宽峰型正态分布,可划分为4个特征差异明显的阶段,反映了大气环境因子对液流的直接驱动效应,以及柠条锦鸡儿叶片气孔行为对液流变化的调控作用。2)液流的变化受多个环境因子共同驱动,其中潜在蒸发散(ET0)综合性强、与Fs相关紧密,是分析液流特征更为可靠的复合型环境变量。Fs与主要环境因子间的耦合关系因时段而异,在早晨启动的液流上升阶段,Fs与太阳辐射、ET0间均呈线性关系,反映了太阳辐射等因子对液流的驱动与对光合作用的激活效应,而在峰值后的下降阶段基本呈Sigmoidal-Hill函数关系;Fs随大气水分亏缺(VPD)的变化与随太阳辐射、ET0的变化趋势相反,表明VPD在达到一定值后启动气孔调节行为以及抑制过度蒸腾耗水的效应。3)柠条锦鸡儿根部Fs与主要环境要素间的变化近似同步,试验期间没有出现典型的时滞现象。4)在整个生长季的大部分时间内观测到柠条锦鸡儿根部夜间持续存在着液流现象,夜间液流量平均占全天液流量的3.83%。试验结果可例证热扩散技术在根部测定液流并用来计算灌木树种单株耗水方法的科学性与优越性。     

6种杀菌剂对西瓜炭疽病的防效试验

采用6种杀菌剂对西瓜炭疽病进行田间药效试验。3次药后试验结果表明:50%咪鲜胺锰盐可湿性粉剂和25%吡唑醚菌酯乳油效果最好,防效在73%以上;10%苯醚甲环唑水分散粒剂和25%溴菌腈可湿性粉剂防效次之,防效在67%以上;50%多菌灵可湿性粉剂和80%代森锰锌可湿性粉剂防效较差,防效为50%左右。药剂处理后,50%咪鲜胺锰盐可湿性粉剂、25%吡唑醚菌酯乳油和对照,中心可溶性固形物含量分别为11.8%、12.0%、9.8%;两种药剂比对照分别增产30.96%、28.26%,增产效果显著。因此,推荐50%咪鲜胺锰盐可湿性粉剂和25%吡唑醚菌酯乳油为目前防治西瓜炭疽病的理想药剂。     

大豆生育期性状QTL定位

选用中豆31×B13杂交组合的182个F2单株构成的群体,构建了一张包含155个SSR标记的简单遗传图谱。利用构建的遗传图谱采用区间作图法对在田间自然条件下大豆的出苗至初花日数、初花至完熟日数和全生育期进行QTL定位,以研究大豆生育期性状的遗传规律。结果发现在C1连锁群上有1个QTL与出苗至初花日数有关,可解释66.40%的遗传变异;发现7个QTL与初花至完熟日数有关,分别位于A2、B2、C1、L、M 5个不同的连锁群上,可解释6.70%~18.00%的遗传变异;2个QTL与全生育期有关,均在C1连锁群上,可分别解释31.90%和36.70%的遗传变异。这些QTL的发现可以为大豆生育期QTL的发掘和利用以及分子辅助育种提供参考。     

酸法提取青稞麸皮结合酚工艺优化

为了提高青稞的附加利用价值,该文以青稞麸皮为研究对象,考察了提取试剂、酸质量分数、萃取pH值、料液比和提取温度对多酚质量分数和 DPPH·自由基清除能力的影响。采用中心组合设计优化其结合酚提取工艺。结果表明,青稞结合酚提取最优工艺条件为：提取试剂为硫酸,酸质量分数为11.10%,水料比为1：17 g/mL,提取温度为75℃,pH值不作处理,此条件下提取得到的结合酚质量分数达224.33 mg/(100 g),DPPH·自由基清除能力达9919.28μmol/(100 g),与预测值多酚质量分数243.80 mg/(100 g),其DPPH·自由基清除能力9087.02μmol/(100 g)基本一致。高效液相色谱法检测到最优工艺下所得青稞结合酚中含有8种酚酸及8种黄酮类物质,总量达325.104 mg/(100 g)。研究结果为全面评价青稞结合酚含量及青稞麸皮的高效利用提供科学依据。