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991.
The release of CO2 by soil microorganisms after the addition of nitrogen and glucose in excess and calibration additions of phosphorus has successfully
been used to assess microbial available P, assuming the native soil P pool is then limiting respiration. However, in P-fixing
soils and soils with high P content, carbon can be exhausted before the available soil P pool. It is not possible to simply
increase the amount of glucose as then the glucose concentration would be lethal for microorganisms. A modified method was
tested where soil is mixed with perlite. It was hypothesised that perlite, having a high water holding capacity, would dilute
the concentration of glucose, while maintaining the bioavailability of added nutrients, thus avoiding carbon limitation. Factorial
combinations of amount of soil and perlite (both adjusted to −25 kPa water potential) were tested to examine if perlite as
such had any effect on the respiration. Five tropical soil samples with a sharp gradient in P availability and one N-limited
compost material were used. The method successfully reduced the risk of carbon limitation. Microbial indices, such as basal
respiration, substrate-induced respiration and maximum P-limited respiration, were directly proportional to the amount of
soil in the experiments but unrelated to the amount of perlite, showing that perlite did not affect microbial measurements. 相似文献
992.
Anthropogenic N-deposition represents a significant input of N into semi-arid chaparral and coastal sage scrub (CSS) shrublands
of southern California. High levels of atmospheric N deposition have the potential to increase soil C and N mineralization,
and we hypothesize that semi-arid shrubland soil exposed to long-term (decades) high N deposition will have significantly
higher C and N mineralization potentials. This hypothesis was tested in a laboratory incubation where the inorganic N (NH4+NO3) and CO2 production of soils maintained at a constant temperature of 25°C and a soil moisture of 0.25 g H2O/g (65% water-filled pore space) were sampled sequentially over a 50-week period. The temporal trend in cumulative C and
N mineralization was well described by a first- and zero-order model, respectively. Long-term atmospheric N deposition significantly
increased potential N mineralization but not C mineralization, and both the rate and total N mineralization were significantly
positively correlated with the surface (0–10 cm) soil δ
15N natural abundance and negatively correlated with the surface soil C:N ratio. While the incubation techniques used here do
not provide realistic estimates of in situ C or N mineralization, these assays indicate that atmospheric N deposition has
significantly altered ecosystem N storage and cycling. 相似文献
993.
Unharvested stubbles or harvested straw of rice (Oryza sativa L.) gets incorporated into soil and interferes with the seedling growth of crop plants. In this paper, we investigated whether
rice straw, either through releasing allelochemicals and/or through manipulating soil properties, influences seedling growth
of Phalaris minor Retz., a non-native weed largely restricted to wheat (Triticum aestivum L.) fields. One hundred twenty grams of soil was amended with rice straw (0.5, 1, 2, 4, or 6 g/pot) and its effect on fresh
shoot biomass of P. minor was examined. Any modification of rice straw phytotoxicity through the use of washed rice straw, activated charcoal, soil
sterilization, or nitrogen fertilization was also studied. We carried out chemical and microbial analysis of soils to examine
the role of soil properties in influencing P. minor growth. Incorporation of rice straw into soil suppressed the growth of P. minor through modifying soil properties. A dose-dependent increase in total phenolics was observed in soil amended with rice straw.
Activated carbon or washing of rice straw, however, could not ameliorate the phytotoxic effects of rice straw. Our results
provide initial evidence that rice straw restricts P. minor growth by manipulating soil chemical and microbiological properties.
Authors contributions IJ conceived of and supervised the study, and wrote the paper; SK carried out the work. 相似文献
994.
Xiao-Ping Jia Yun-Su Shi Yan-Cun Song Guo-Ying Wang Tian-Yu Wang Yu Li 《Genetic Resources and Crop Evolution》2007,54(2):233-236
The development of EST-SSR in foxtail millet (Setaria italica) for polymorphism and transferability study was reported here. From 1213 EST sequences, 30 SSRs were obtained and primers
were designed for 26 SSRs. Among them, four pairs of SSR primers amplified polymorphic products in 12 foxtail millet cultivars
and one accession of Setaria viridis, a wild relative of foxtail millet, with 10 alleles detected for the four loci and 2.5 alleles per locus. In addition, ten
SSR markers could be transferred to other nine Gramineae species. The putative functions of 11 ESTs containing polymorphic
and transferable SSRs were also identified. 相似文献
995.
Elizabeth Rice 《Genetic Resources and Crop Evolution》2007,54(4):701-713
Using survey data from Jala, Mexico, this case-study evaluates in situ maize conservation of the variety ‘Jala’ (Zea mays L.). Though historically ‘Jala’ was the dominant variety grown in the valley of Jala, today less than 20% of farmers grow
it on only 5% of the maize area. Younger growers of the ‘Jala’ variety specialize in it, growing relatively large amounts
for niche markets. Older, diversified farmers grow small areas for household use and to compete in a local contest. Conservation
of the ‘Jala’ variety has been heavily influenced by shifting ideal concepts of maize, as determined by market and consumption
demands and by a contest designed to promote in situ conservation. The current move away from nationalized purchasing may
favor ‘Jala’s’ continued conservation. 相似文献
996.
In terrestrial ecosystems, gross nitrogen mineralisation is positively correlated to microbial biomass but negatively to soil
organic matter C-to-N ratios; the influence of the microbial community structure is less well known. Here, we relate rates
of gross N mineralisation to fungi-to-bacteria ratios in three natural forest types of contrasting N availability and in a
long-term N-loading experiment in a boreal forest. We report, for the first time, a strong negative correlation between gross
N mineralisation and the fungi-to-bacteria ratio ( = 0.91, P = 0.0005, N = 7). There was also a negative correlation between gross N mineralisation and the C-to-N ratio ( = 0.89, P = 0.001, N = 7), but a weaker positive correlation between gross N mineralisation and soil pH ( = 0.64, P = 0.019, N = 7). Our analysis suggests that soil fungi-to-bacteria and C-to-N ratios are interrelated and that they exert strong influences
on soil N cycling in boreal forests. 相似文献
997.
Vinod Kumar Yadav Sandeep Kumar Ravindra K. Panwar 《Genetic Resources and Crop Evolution》2007,54(6):1285-1289
The present investigation was carried out on fifteen germplasm lines of Pisum sativum L. were used for characterization using Randomly Amplified Polymorphic DNA (RAPD) markers. While 12 random primers were taken,
out of them 11 primers gave amplification. These primers gave a total of 133 bands out of which 106 were polymorphic. Genetic
similarities of the RAPD profiles were estimated by using Jaccard’s coefficient with NTSYSpc 2.0 software. The similarity
index values ranged from 0.263 to 0.793 indicating the presence of enormous genetic diversity at molecular level. A dendrogram
generated by cluster analysis divided fifteen fieldpea genotypes into two Groups A and B. Major Group A have five genotypes
and major Group B have nine genotypes. 相似文献
998.
In industrial areas, heavy metals may accumulate in forest soil organic horizons, affecting soil microorganisms and causing
changes in the chemical composition of the accumulated organic matter. The objectives of this study were to test the ability
of near-infrared spectroscopy (NIRS) to detect heavy metal effects on the chemical composition of forest soil O horizons and
to test whether NIRS may be used to quantitatively determine total and exchangeable concentrations of Zn and Pb (Znt, Pbt, Znex, Pbex) and other chemical and microbial properties in forest soil O horizons polluted with heavy metals. The samples of O horizons
(n = 79) were analyzed for organic C (Corg), total N and S (Nt, St), Znt, Pbt, Znex, Pbex, basal respiration (BR), microbial biomass (Cmic) and Cmic-to-Corg ratio. Spectra of the samples were recorded in the Vis-NIR range (400–2,500 nm). To detect heavy-metal-induced changes in
the chemical composition of O horizons principal components (PC1–PC7) based on the spectral data were regressed against Znt + Pbt values. A modified partial least squares method was used to develop calibration models for prediction of various chemical
and microbial properties of the samples from their spectra. Regression analysis revealed a significant relationship between
PC3 and PC5 (r = −0.27 and −0.34, respectively) and Znt + Pbt values, indicating an effect of heavy metal pollution on the spectral properties of the O horizons and thus on their chemical
composition. For quantitative estimations, the best calibration model was obtained for Corg-to-Nt ratio (r = 0.98). The models for Corg, Nt, and microbial properties were satisfactory but less accurate. NIRS failed to accurately predict St, Corg-to-St, Znt, Pbt, Znex, and Pbex. 相似文献
999.
The effect of soil freeze–thaw cycles on the denitrification potential was examined based on the C2H2 inhibition method. The gross N2O production curve of the soil sample (incubation with C2H2) showed minor changes between the freeze–thaw treatment and the unfrozen control. However, kinetics analysis revealed that
the initial production rate, an indicator of the population density of denitrifying communities, decreased (P = 0.043) and the specific growth rate constant, an indicator of the activity of denitrifying communities, increased (P = 0.039) as a result of the freeze–thaw cycles in five of six soil samples examined. The increase in the specific growth
rate constant suggested the stimulation of the activity of denitrifying communities that survived after the freeze–thaw cycles
and may explain the minor suppression on the gross N2O production in spite of decreasing the population density of denitrifying communities that was suggested by the initial production
rate. The net N2O production curve of the soil sample (incubation without C2H2) showed a remarkable change in one out of six soil samples, and in that one soil sample, N2O release to the atmosphere was largely stimulated (7.6 times) by the freeze–thaw cycles. However, the stimulation of the
N2O release by the freeze–thaw cycles was even observed in two other selected soil samples (4.6 and 1.8 times), suggesting that
an imbalance in the N2O-producing and N2O-reducing activities of denitrifying communities might complementally explain the N2O release stimulated by the freeze–thaw cycles. 相似文献
1000.
A. de Varennes M. O. Torres C. Cunha-Queda M. J. Goss C. Carranca 《Biology and Fertility of Soils》2007,44(1):49-58
We investigated conservation and cycling of N under oat–oat and lupine–oat rotations in disturbed and undisturbed soil, when
roots or roots plus aboveground residues were retained. Crop residues were labelled with 15N in Year 1, and differential soil disturbance was imposed after harvest. In Year 2, plant growth, N transfer from residue
into the various sinks of the second crop (plant, soil, and residual residues), and changes in microbial activity and numbers
were determined. Oat biomass was greater after lupine than after oat due to differences in supply of N from these residues.
Buried residues of both crops appeared to decompose faster than when left on the soil surface. Lupine residues decomposed
faster than oat residues. Oat biomass was not affected by soil disturbance if grown after lupine but decreased when oat straw
was buried in the soil. More residue N was recovered from soil than from the crop. Most 15N was recovered from disturbed soil, which also had greater dehydrogenase activity and more culturable fungi. At the end of
the oat–oat rotation, 20 and 5 kg N ha−1 were derived from the roots of the first crop in undisturbed or disturbed soil, respectively. Equivalent values for the lupine–oat
rotation were 18 and 44 kg N ha−1. Returning aboveground residues provided an extra 52–80 kg N ha−1 for oat and 61–63 kg N ha−1 for lupine relative to treatments where they were removed. Over a year, lupine contributed 9 to 20 kg N ha−1 more to the agroecosystem than did oat. 相似文献