天然提取物对珙桐茎诱导愈伤组织的影响

[目的]筛选诱导珙桐茎愈伤组织的最佳培养基,为建立悬浮细胞系以及大规模繁殖珙桐提供前期技术基础。[方法]采集5年生以下珙桐的嫩枝条幼嫩茎为外植体,环割后接种在改良MS培养基上,培养基设25个处理,分别添加浓度为0、0.1、0.2、0.3、0.4 mg/L的NAA,浓度为0、10、20、30、40 mg/L的豆芽汁和浓度为0、5、10、15、20 mg/L的番茄汁。[结果]天然提取物和植物生长调节剂对珙桐茎诱导愈伤组织都有影响,其影响程度是NAA>豆芽汁>番茄汁。改良MS培养基添加30 mg/L豆芽汁+20 mg/L番茄汁+0.4 mg/LNAA是诱导珙桐茎愈伤组织的最佳培养基,诱导出的愈伤组织质量好,出愈率高达98%。[结论]添加天然提取物可诱导出质量好的珙桐茎愈伤组织,这与豆芽汁和番茄汁含有丰富的维生素B和维生素C有关。     

鸢尾属部分园艺品种遗传多样性的ISSR分析

以34份鸢尾属园艺品种为试材,采用ISSR分子标记方法,研究鸢尾属园艺品种的遗传特性及亲缘关系。结果表明:10个ISSR随机引物检测到153个多态位点,多态性条带比例为100%,表明供试材料具有丰富的遗传多态性。各品种之间的DICE遗传相似系数在0.148~0.657之间,平均相似系数为0.365。由聚类分析可知,当遗传系数为0.354时,可将供试材料分为4个品种群。其中Ⅰ群有21个品种,全部为须毛状附属物亚属的德国鸢尾。Ⅱ群有4个品种,包括无附属物亚属的西伯利亚鸢尾和2个花菖蒲品种。Ⅲ群有4个品种,包括2个花菖蒲品种以及2个从荷兰引进的德国鸢尾。Ⅳ群为无附属物亚属的路易斯安那鸢尾品种群。主坐标分析所反映的关系和UPGMA聚类图相似。     

Effect of gonadotropin-releasing hormone analogue on human breast cancer cell line in vitro

AIM: To investigate the effects of gonadotropin-releasing hormone (GnRH) analogue on the growth of breast cancer cell lines MCF-7 and MDA-MB-231 in vitro and to explore the related mechanisms with PI3K/Akt or ERK/MAPK pathways. METHODS: The proliferation of human breast cancer cell lines MCF-7 and MDA-MB-231 treatment with triptorelin was detected by MTT assay and the distribution of the cell cycle was determined by flow cytometry. The phosphorylation of the ERK1/2 and Akt was evaluated by Western blotting. RESULTS: Triptorelin inhibited the proliferation of MCF-7 cells at concentration of 10^-5 mol/L after treated for 192 h or at concentration of 10^-4 mol/L after treated for 168 h and 192 h. Triptorelin inhibited the proliferation of MDA-MB-231 cells at concentration of 10^-4 mol/L after treated for 192 h (P<0.05).Treatment with triptorelin for 192 h at concentration of 10^-4 mol/L had no statistical significance effect on phosphorylation of ERK1/2 and Akt(P>0.05).CONCLUSION: Inhibitory effect of GnRH analogue triptorelin on human breast cancer cells is not just the connection with the down-regulation of pituitary hormone, but also a direct inhibitory effect. The role may not be involved in the activation of ERK/MAPK and PI3K/Akt signaling pathways.     

Downregulation of DEK induces cell apoptosis via inhibition of NF-κB signaling pathway in gastric carcinoma SGC-7901 cells

AIM: To investigate the effect of DEK downregulation on the apoptosis of gastric carcinoma SGC-7901 cells, and to explore its associations with NF-κB signaling pathway and apoptosis related proteins. METHODS: SGC-7901 cells with different treatments were divided into 3 groups including untreated group, control siRNA group and DEK siRNA group. The expression of DEK at mRNA and protein levels in the SGC-7901 cells was detected by real-time PCR and Western blot. The cell apoptosis was examined by flow cytometry. Furthermore, the activities of caspase-3 and caspase-9 in the SGC-7901 cells were investigated by Caspase-Glo^®-3/9 kit. Finally, the expression of key regulatory protein p65 of NF-κB signaling pathway and apoptosis-related proteins Bcl-2 and Bax in the SGC-7901 cells was investigated by Western blot. RESULTS: Compared with untreated group and control siRNA group, the expression of DEK at mRNA and protein levels was significantly downregulated in DEK siRNA group (P<0.05). In addition, the ratios of early phase apoptosis and total apoptosis in DEK siRNA group were markedly higher than those in untreated group and control siRNA group (P<0.05). Most notably, the decrease in p65 and Bcl-2 proteins, increase in Bax protein and the increases of caspase-3 and caspase-9 activities were observed in DEK siRNA group. CONCLUSION: Downregulation of DEK mediates cell apoptosis of gastric carcinoma may be tightly associated with NF-κB signaling pathway.     

日光温室番茄品种比较试验

以‘多彩’、‘L30’、‘L31’、‘L40’、‘天正粉奥’、‘LC-10’6个番茄品种为试材,当地畅销品种‘LC-10’为对照,研究比较了不同番茄品种在日光温室中的植株性状、抗病性和产量及品质等性状。结果表明:6个番茄品种的植株性状、抗病性和产量都有一定程度的差异;其中‘多彩’优势明显,果实商品性状好,始花节位最低,株高和节间长度适中,花穗间叶片数较多并且茎粗最粗;果实扁圆形,果形端正,着色均匀,比对照增产17%,抗病、耐储运,为日光温室优良番茄品种。     

棉花不同节位棉铃纤维品质差异性研究初报

2005-2006年按棉株不同节位研究了棉花纤维品质三项主要指标的差异性.结果表明:(1)果节由内而外,麦克隆值表现为由高到低,同位铃间麦克隆值无显著差异.(2)1～3果枝不同节位棉铃纤维上半部平均长度由内向外逐渐变短,4果枝以上不同节位由内向外逐渐变长.(3)断裂比强度1～3果枝不同节位棉铃由内向外逐渐变低,5果枝以上不同节位棉铃由内向外逐渐变高.     

不同粒型花生品种衰老过程中生理特性的变化

以不同粒型的花生品种为材料，研究衰老过程中叶片的生理特性及产量性状。结果表明：后期叶片NR、PAL的活性呈下降趋势，但大粒型品种较小粒型品种下降速度慢；开花50d以后，大粒型品种AsA-POD的变化呈抛物线下降（Y=-0．0492X^2＋5．5791X-146．82）；小粒型品种呈直线下降（Y=-0．553X＋39．302）。MDA含量在开花后60—65d迅速上升，至成熟期，大粒型品种MDA含量较小粒型品种降低44．9％，百仁重、百果重平均分别增加46．8％、61．4％，产量提高33．2％。     

牛GDF-9基因的克隆与组织表达的分析

为了检测牛GDF-9基因mRNA在怀孕期牛生殖系统的表达情况,为GDF-9基因在雌性动物繁殖上的研究提供一定的理论依据。本试验根据GenBank中报道的牛GDF-9基因序列设计引物,从牛卵巢、输卵管、子宫、肾脏中提取RNA,采用RT-PCR技术进行cDNA扩增,扩增产物与载体pGM-T连接,转化大肠杆菌DH5α,筛选阳性克隆,测序。结果表明,已成功克隆的阳性重组子经测序鉴定其片段大小与预期大小完全一致,为264 bp。序列同源性分析比较表明,该cDNA及其推导的氨基酸序列与绵羊GDF-9cDNA和氨基酸序列同源性分别为97%和94%。以-βactin为内参照物,采用RT-PCR技术进行半定量分析,通过检测比较牛GDF-9基因在卵巢、输卵管、子宫、肾脏组织中mRNA的表达情况。结果表明,牛GDF-9基因在卵巢中表达量最高,在输卵管、子宫、肾脏中均有表达,但表达量不高。     

作物产量“三合结构”定量表达及高产分析

针对目前作物产量水平长期徘徊难以突破，产量分析理论缺乏量化指标体系，可操作指导作用小等问题，依据“三合结构”模式二级结构层各因素的关系，建立了“三合结构”定量表达式，并通过田间试验与模型模拟相结合的方法，对春玉米、夏玉米、水稻和冬小麦高产实例进行定量化分析，明确了限制产量进一步提高的关键因素，提出了高产突破的可能方向。结果表明，提高叶片平均净同化率（MNAR），改善群体的物质生产能力，是水稻产量进一步提升的关键；适当提高平均叶面积指数（MLAI）或经济系数（HI）可能会进一步增加冬小麦产量；春玉米籽粒产量主要伴随着MLAI和单位面积穗数（EN）的增加而提高，其实质是平均作物生长率（MCGR）的提高增加了单位面积上总粒数（TGN）。进一步研究确定了“三合结构”定量表达式参数间的函数关系式，通过公式代换可推导出某一参数与目标参数的函数关系。作物产量“三合结构”定量表达式的建立为作物群体定量化研究提供了新的思路和方法，有助于全面掌握群体参数变化与产量形成的定量关系，为指导作物生产进行有效的技术调控提供依据。     

Mechanical Performance and Thermology Performance of 20CrMo Steel at High Temperature

Employing the Gleeble-1500D thermal analogue machine and the STA449 synthesis thermal analyzer,the study on hot ductility and parts of thermology performance of 20CrMo steel has been carried out , many consulted foundation data has been obtained, the optimum ductility temperature arrange has been found, contraction of cross sectional area is only 30% between 600 degree and 775 degree, but contraction of cross sectional area is more than 80% between 800 degree and 1 250 degree, contraction of cross sectional area drops to under 60% rapidly between 1 350 degree and melting point,a curve is drawn showing the relationshi Pbetween heat capacity and the variation of temperature for the first time, the counting formulas of the heat capacity are regressed by disjunction. A curve is drawn showing the relationshi Pbetween the coefficient of thermal expansion and the variation of temperature, and their application in continuous casting is discussed herein.