Advances on Detection Methods for Residues of Nitrofurans in Food

Nitrofurans are broad-spectrum antibiotics which have been widely used in food animals to the prevention and treatment of gastrointestinal infections.Due to abuse of drugs by criminals,animals could produce drug-resistant strains,which reduces the role of antibiotics.Meanwhile,excessive drugs residues in food has carcinogenic,teratogenic and mutagenic effects to the human body.In this paper,the damage and the detection methods of the residues of the nitrofurans in food are surveyed.The main detection methods include high performance liquid chromatography,liquid chromatography-mass spectrometry,liquid chromatography tandem mass spectrometry,enzyme-linked immunoassay,colloidal gold immunoassay and fluorescence immunity analysis method.The research status of various detection methods are summarized and the suggestions for further research are put forward.It is expected to provide reference for future research.     

Identification of Suspected Stem/progenitor Cells in Bovine Mammary Epithelial Cells Culture System

To develop the potential function of dairy cow mammary stem cells (DCMECs) in regulation of lactation,we identify putative DCMECs which were BrdU label retaining epithelial cells,at the same time,analysis the location of two new mammary stem cells molecular marks FNDC3B and PROCR to verify the feasibility of them to indicate DCMECs.The mRNA levels of prolactin,growth hormone,insulin-like growth factor-1 and their receptors were detected along with cell passage by Real-time quantitative PCR.The results showed that the proportion of BrdU label-retaining epithelial cells was nearly 0.4% after 25 d continuous culture (passaged 4 times) and few cells were positive for FNDC3B or PROCR.Moreover,we observed the BrdU labelled epithelial cells by asymmetric division.The mRNA levels of prolactin,growth hormone,insulin-like growth factor-Ⅰ and their receptors in primary and passage cells were extremely significant difference(P<0.01).DCMECs would rapidly lose some physiological characteristics and the ability of milk synthesis when not under the condition of induction of lactation differentiation,but a certain percentage of mammary stem/progenitor cells will be retained,whose potential effects on the regulation of lactation and mammary acinar remodeling were worthy of attention.     

RFID技术应用于古籍管理的利弊分析

RFID技术的应用为图书馆的古籍管理带来了便利,同时也面临应用上的不适与困境。从利弊两个方面来展开探讨RFID技术在古籍管理中的应用现状,并提出解决措施,同时对未来提出展望。     

大豆皂苷Bb乙二胺衍生物的制备

天然的皂苷分子结构不一定表现出免疫佐剂活性的最佳状态,为获得不同皂苷结构修饰衍生物,研究选用碳二亚胺法,借助于乙二胺衍生物的氨基,使大豆皂苷单体Bb分别与β-苯丙酸、L-脯氨酸发生缩合反应,将亲脂性的,带正电荷的、亲水性的化合物基团分别连接到大豆皂苷C-3-O位的葡萄糖醛酸羧基上,形成不同程度亲水、亲脂性的衍生物。分别使用分析及制备型HPLC分离纯化制备的衍生物,并利用傅立叶红外光谱、质谱等方法鉴定衍生物结构。研究结果形成了不同结构的衍生物,可用于后续的大豆皂苷结构与免疫佐剂活性的研究。     

内蒙古草原污染源及对畜体危害分析

随着内蒙古草原牧区工农业、旅游业的发展,草原生态受到了一定影响。工业及生活污染日趋严重,造成放牧场牧草污染,污染残留物的转移和积蓄也影响了家畜正常健康的生长。简单概述了草原牧草的污染源、污染元素及对畜体危害。     

蚕豆耐盐性的研究进展

综述了盐胁迫对蚕豆生长和生理响应的影响及提高蚕豆耐盐性方法等方面的研究进展,旨在为进一步揭示蚕豆耐盐生理和分子遗传机制及培育耐盐蚕豆新品种提供参考依据,并对今后蚕豆耐盐性的研究方向进行展望。     

基于最优投影逼近法的X射线硬化校正

射束硬化是导致CT重建图像下降的物理原因之一。该文分析了射束硬化校正的常用方法,比如线性化方法、基于原始投影正弦图的校正方法,并在此基础上提出了一种适用于非圆形检测对象的最优投影逼近法。该方法将射线穿透距离最短的扫描角度作为最优投影角度,将该角度下的原始投影作为校正标准,并建立硬化校正方程,通过牛顿迭代法解得各个投影角度下的校正值。实验结果表明,采用本文方法进行硬化校正后,由射束硬化引起的杯状伪影得到较好地抑制。该方法具有自适应性,执行效率高,并且可以推广到多材质扫描对象的硬化校正。     

山东省冬小麦2001-2010年生长季变化分析

从中国近年来农作物生长发育状况资料数据集提取山东省2001-2010年的主要农作物重要物候期,包括生长季开始时间、结束时间和生长季长度,分析了10a间山东省主要农作物物候期的年际变化趋势和空间分异特征。结果表明:(1)山东省冬小麦生长季为2月下旬至6月上旬,平均生长季长度为98d。2001-2010年山东省冬小麦的生长季开始时间显著推迟,结束时间有提前趋势,但提前不显著,生长季有所缩短,10a缩短约5d;(2)不同地区冬小麦物候特征差异较大,分析菏泽、惠民、文登、临沂等4个典型研究区冬小麦的物候年际变化发现,菏泽、惠民、文登等地区生长季长度有缩短趋势,只有临沂地区生长季有延长趋势,且延长变化不显著。     

基于熵权的模糊物元模型在城市人居环境质量综合评价中的应用

基于城市人居环境质量优劣的模糊性和单项指标评价结果的不相容性,在物元分析的基础上,结合模糊集理论和欧氏贴近度概念,建立了城市人居环境质量评价的模糊物元模型,并应用信息熵所反映数据本身的效用值来计算指标的权重系数,有效避免了权重分配困难的问题和主观判断城市人居环境质量优越标准的不确定性。实例研究表明,运用熵模糊物元模型评价城市人居环境质量状况,方法是可行的,结论是可靠的。     

沼肥在温室蔬菜生产中的应用和发展势趋

总结了近几年沼肥技术在农业生产中的应用,如配营养土盆栽、预防农作物病虫害、沼肥改良土壤、作为肥料代替化肥等。其中用得最多、最好的是将其作为肥料直接用于粮食和蔬菜生产。业已证明,沼肥具有提高蔬菜产量、改善蔬菜品质、防治病虫害的功效,为生产无公害蔬菜提供了一条很好的途径。