黄瓜种质资源遗传亲缘关系的RAPD分析

利用ＲＡＰＤ技术对多个生态型的黄瓜材料的遗传亲缘关系进行了研究。从２００个１０碱基随机引物中筛选出２０个用于ＰＣＲ反应,３９．２％的扩增条带表现多态性;每个生态型品种都具有其特有的扩增（缺失）带以区别其他生态型品种;聚类分析将供试材料分为３大类群：华北类群、华南类群和欧洲温室类群。从分子水平验证了传统的黄瓜地域分类标准及黄瓜是遗传基础狭窄的蔬菜作物。对利用ＲＡＰＤ技术进行亲缘控制研究的可能性进行了探讨。     

西瓜野生种质耐冷性基因连锁的RAPD标记

运用ＲＡＰＤ技术进行了西瓜野生材料ＰＩ４８２３２２耐冷性基因连锁的分子标记的研究,找到了一个分子连锁标记ＯＰＧ１２／１９５０与耐冷基因连锁,其遗传距离为６．９８ｃＭ。     

Research Advances in Escherichia coli K1 Penetration Across Blood-brain Barrier

E.coli K1 strain is a representative strain of neonatal sepsis and meningitis, which causes disease by blood circulation to the brain. The molecular mechanism of E.coli K1 that adhering and invading the brain microvascular endothelial cells (BMECs) and cross the blood brain barrier (BBB) has been focused on by many scholars. In this review, we focused on the gene regulation mechanisms and signaling pathway of E.coli K1 crossing the blood brain barrier to understand the molecular mechanism of the infection caused by E.coli and provide reference for prevention and treatment programs.     

獭兔被毛检测仪的研制与开发初报

以现代光电仪器及计算机科学和技术原理与方法,开发将实物描述为图像,建立数学模型转换为数据读出结果的软件；研制取样系统、显像系统、识别系统、显示系统、传导系统,并进行便携、实用、美观的部件加工、组装、调试,成功研制出了獭兔皮毛长度、直径、密度快速检测仪.试用结果精准度优于切片检测,为獭兔和毛皮动物毛皮质量的检测提供了先进的仪器和检测技术.     

2012/13年度大豆市场回顾与2013/14年度展望

2012/13年度大豆市场的表现与2011/12年度迥异,增产预期在多数时间里抑制着价格,使得2012/13年度的大豆价格在2011/12年度屡创新高之后盛极而衰,此前活跃的需求因素陷入沉寂也是不应被忽视的重要原因。展望2013/14年度,除了预期继续增产可能带来的压力之外,需求形势及其背后局势的演变有望成为重要看点,预计会给大豆市场带来更多不确定性,这可能成为影响价格走势的X因素,初步预计价格将宽幅震荡。     

关于水力报警阀的几点看法

本文主要论述了水力报警阀的工作原理及其应用。     

Effects of STK15 overexpression on growth of human esophageal carcinoma cell line KYSE150

AIM:To investigate the effects of serine/threonine kinase 15 (STK15) overexpression on the growth of human esophageal squamous-cell carcinoma (ESCC) cell line KYSE150. METHODS:Recombinant pEGFP-C1-STK15 expression vector was transfected into KYSE150 cells using LipofectamineTM 2000 and the expression of STK15 was detected by fluorescence microscopy and Western blotting. The proliferation of the cells in vitro was measured by MTT assay. The cell cycle distribution and apoptosis were detected by flow cytometry. The proliferation of the cells in vivo was measured by tumorigenicity experiment in nude mice. RESULTS:After recombinant pEGFP-C1-STK15 expression vector was stably transfected into KYSE150 cells, GFP-STK15 fusion protein localized to centrosome and spindle. The STK15-overexpressing colonies were further confirmed by Western blotting. MTT assay showed that the proliferation of the cells in STK15 overexpression group was increased compared with control group (P<0.01). Flow cytometry analysis showed that the percentage of the cells in G0/G1 phase and the cell apoptosis in STK15 overexpression group were decreased compared with control group (P<0.01). The tumorigenicity experiment in nude mice showed that the proliferation of the cells in STK15 overexpression group was increased compared with control group (P<0.01). CONCLUSION: Overexpression of STK15 in human ESCC KYSE150 cells promotes the cell growth in vitro and in vivo, indicating that STK15 may serve as a novel therapeutic target for esophageal carcinoma.     

宝鸡市豆类产业现状及发展对策探讨

通过对宝鸡市豆类产业化现状的分析研究,找出了存在问题和不足,并针对性地提出了今后的发展对策,以促进豆类产业健康、快速向前发展.     

城市公园地域特色塑造探析——以泗阳生态公园规划为例

作为城市的有机组成部分,相对于主要由人工系统组成的城市的其他部分,如道路、建筑等基础设施,城市公园的自然性和文化性更为凸显,城市公园对展现城市的地域特色具有不可替代的重要作用.城市公园地域特色塑造的关键问题在于立足于现状,把握地域在自然、文化和经济技术等方面的特点,根据各城市不同的情况,因地制宜,把握时代性,认真探求不同地域不同城市具有地域特色的城市公园建设之路.     

雪腐格氏霉粗毒素导致小麦叶组织膜渗透性和超微结构的变化初报

 雪腐格氏霉[Gerlachia nivalis,异名:雪腐镰刀菌(Fusarium nivalis)]是引致小麦雪霉叶枯病的病原菌,国内外学者均有该菌能够产生毒素(Gn-毒素)的报告。本试验就该菌毒素对不同抗性小麦品种叶组织细胞膜渗透性和组织超微结构的影响进行了初步研究,为进一步研究该病菌的致病机理奠定了基础。