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11.
Tabouret G Lacroux C Andreoletti O Bergeaud JP Hailu-Tolosa Y Hoste H Prevot F Grisez C Dorchies P Jacquiet P 《Veterinary research》2003,34(2):231-241
Cellular and humoral local responses were investigated following repetitive artificial Oestrus ovis infections in lambs. The presence of larvae induced a huge local recruitment of either leucocytes (T and B lymphocytes, macrophages) or granulocytes (eosinophils, mast cells and globule leucocytes). This cellular response was more pronounced in the ethmoid and sinus (development sites of second and third instar larvae) than in the septum or turbinates where first instar larvae migrate. Infected lambs produced Oestrus ovis specific IgG and IgA antibodies in their mucus. This local humoral response was mainly directed against larval salivary gland antigens and not against larval digestive tract antigens. Compared to the control animals, the sinusal mucosa of infected animals was extremely thickened and the epithelium exhibited hyperplasia, metaplasia and eosinophilic exocytosis. The possible roles of these local immune responses in the regulation of O. ovis larvae populations in sheep are discussed. 相似文献
12.
Cytotoxic constituents from Plumbago zeylanica 总被引:5,自引:0,他引:5
Nguyen AT Malonne H Duez P Vanhaelen-Fastre R Vanhaelen M Fontaine J 《Fitoterapia》2004,75(5):500-504
The bioassay-guided fractionation of the dichloromethane extract of aerial parts of Plumbago zeylanica led to the isolation of beta-sitosterol, beta-sitosteryl-3beta-glucopyranoside, beta-sitosteryl-3beta-glucopyranoside-6'-O-palmitate (1), lupenone, lupeol acetate, plumbagin and trilinolein. Compound 1 showed cytotoxic activity against MCF7 and Bowes cancer cell lines (IC50 113 microM and 152 microM, respectively), beta-sitosterol inhibited Bowes cell growth (IC50 36.5 microM) and plumbagin was cytotoxic against MCF7 and Bowes cells (IC50 1.28 microM and 1.39 microM, respectively). 相似文献
13.
Christelle Navarro Nolwenn Crastes Elodie Benizeau David McGahie 《Irish veterinary journal》2015,68(1)
Background
Atopic dermatitis (AD) is the most common canine allergic skin disease and can significantly affect the quality of life of affected dogs. Treating canine AD with ciclosporin has been a subject of great interest in recent years. Many studies have provided substantial evidence of ciclosporin efficacy and safety in canine AD management, and for several years ciclosporin has been recognised as a major component of canine AD multimodal therapy. As a chronic condition, canine AD requires life-long medical management and treatment success relies in large part on product ease of administration. Two studies were conducted to assess the comparative voluntary acceptance and consumption of Cyclavance® (Virbac), a new oral liquid formulation of ciclosporin, and Atopica® (Novartis) either added to a small quantity of kibbles (study 1) or administered directly into the dog’s mouth (study 2).Results
Over the course of the two studies 70 dogs assessed each of the ciclosporin formulations and 320 individual tests were performed for each tested product. Immediate prehension (in less than 2 seconds) occurred significantly more often with Cyclavance® (90.6% of the tests) than with Atopica® (14.4% of the tests) when products were mixed with 30 grams of dry food (p < 0.001). Moreover, Cyclavance® was significantly more often easily accepted than Atopica® (99.3% vs 27.1% of the tests, respectively) when products were administered directly into the dogs’ mouth (p < 0.0001). Cyclavance® was also more often totally consumed (98.3% of the tests) than Atopica® (2.2% of the tests) when mixed with a small amount of food (p < 0.001). However, both products were totally consumed once administered directly into the dogs’ mouth.Conclusions
By facilitating cicloporin administration and consumption, Cyclavance® liquid formulation offers an interesting alternative to capsules that may improve dosing compliance and therefore the ability to benefit from the therapeutic effects in the long-term treatment of canine AD. 相似文献14.
Bienvenu Kambashi Christelle Boudry Pascale Picron Honoré Kiatoko Jérôme Bindelle 《Tropical animal health and production》2014,46(8):1497-1502
The effects of four tropical forage legume hays (Vigna unguiculata, Psophocarpus scandens, Pueraria phaseoloides and Stylosanthes guianensis) on voluntary feed intake (VFI) and their nutritive value were studied in growing pigs using a corn-soybean meal-based diet containing varying proportions of forage legume hays (0, 10, 20 and 40 % or 0, 12.5 and 25 % for VFI and nutritive value determination, respectively). There was no difference in VFI between species (P?>?0.20), but a linear response to forage inclusion level (P?0.05) was observed decreasing from 126 for 0 % to approximately 84 g/kg of body weight for the 40 % forage diets, except for V. unguiculata, where the response was quadratic (P?=?0.01). All four forage species linearly decreased the total tract apparent digestibility (TTAD) from 0.76 to 0.61, 0.80 to 0.68, 0.54 to 0.40 and 0.58 to 0.31 except for S. guianensis (0.44) for DM, N, NDF and N retention, respectively. Differences in digestibility (P?0.05) between species were also observed. Due to their negative influence on the overall digestibility, the contribution of hays should not exceed 12.5 %, except for S. guianensis, in which N retention remained quite high (0.44) at the highest inclusion level (25 %). P. phaseoloides hay should be avoided in pigs as it combines the lowest VFI with the lowest nutrient digestibility. 相似文献
15.
16.
Guillaume Sallé Carole Moreno Simon Boitard Julien Ruesche Aurélie Tircazes-Secula Frédéric Bouvier Mathias Aletru Jean-Louis Weisbecker Fran?oise Prévot Jean-Paul Bergeaud Cathy Trumel Christelle Grisez Emmanuel Liénard Philippe Jacquiet 《Veterinary research》2014,45(1):68
This study reports a functional characterization of a limited segment (QTL) of sheep chromosome 12 associated with resistance to the abomasal nematode Haemonchus contortus. The first objective was to validate the identified QTL through the comparison of genetically susceptible (N) and resistant (R) sheep produced from Martinik × Romane back-cross sheep. The R and N genotype groups were then experimentally infected with 10 000 H. contortus larvae and measured for FEC (every three days from 18 to 30 days post-challenge), haematocrit, worm burden and fertility. Significant differences in FEC and haematocrit drop were found between R and N sheep. In addition, the female worms recovered from R sheep were less fecund. The second step of the characterization was to investigate functional mechanisms associated with the QTL, thanks to a gene expression analysis performed on the abomasal mucosa and the abomasal lymph node. The gene expression level of a candidate gene lying within the QTL region (PAPP-A2) was measured. In addition, putative interactions between the chromosome segment under study and the top ten differentially expressed genes between resistant MBB and susceptible RMN sheep highlighted in a previous microarray experiment were investigated. We found an induction of Th-2 related cytokine genes expression in the abomasal mucosa of R sheep. Down-regulation of the PAPP-A2 gene expression was observed between naïve and challenged sheep although no differential expression was recorded between challenged R and N sheep. The genotyping of this limited region should contribute to the ability to predict the intrinsic resistance level of sheep. 相似文献
17.
Sylvie Dufour Maïté Montero Nadine Le Belle Marc Bassompierre Judy A. King Robert P. Millar Richard E. Peter Yves-Alain Fontaine 《Fish physiology and biochemistry》1993,11(1-6):99-106
Using specific radioimmunoassays for the two GnRH molecular forms present in the European eel, Anguilla anguilla, (mGnRH and cGnRH II), we compared their distributions in the pituitary and different parts of the brain of female silver eels, as well as the modifications of their levels in experimentally matured female eels (treated with carp pituitary extract). In control eels, mGnRH levels were higher than cGnRH II levels in the pituitary, olfactory lobes and telencephalon, di- and mesencephalon, while the opposite was found in the posterior part of the brain (met- and myelencephalon). Experimental sexual maturation of the gonads significantly increased mGnRH levels in the pituitary and anterior parts of the brain; such a positive effect was not observed on the low cGnRH II levels, which were, in contrast, reduced. These data indicate that the positive feedback of gonadal hormones on GnRH, that we previously demonstrated, would specifically affect the mGnRH form. The differential distribution and control of mGnRH and cGnRH II suggest that these two forms have different physiological roles in the eel. The large increase in mGnRH during sexual maturation suggests the prime implication of this form in the neuroendocrine control of reproduction. 相似文献
18.
N. Wang H. Migaud L. Acerete J.N. Gardeur L. Tort P. Fontaine 《Fish physiology and biochemistry》2003,28(1-4):523-524
Perch breeders showed high mortality rates during the spawning season. This could be linked to immunosuppressive effects of environmental conditions or reproduction. 相似文献
19.
20.
We tested whether synchronous, long‐term fluctuations in Atlantic bluefin tuna (Thunnus thynnus) trap catches, collected from the ancestral Mediterranean and Atlantic trap fishery, might be related to large‐scale environmental change. Nine time series of trap catches of more than 80 yr long were compared with long time series of three preselected environmental variables, i.e. the North Atlantic Oscillation (NAO), the Length of the Day Index (LOD, a proxy of the atmospheric circulation index) and the temperature. Spectral analyses of trap catches, LOD and temperature displayed similar spectra with peaks at low frequencies, whereas those of the NAO exhibited a broad band spectrum. Regression analyses and tests of correlation did not reveal any clear relationship between trap catches on the one hand and NAO and LOD on the other hand. In contrast, long‐term fluctuations in trap catches appear to be closely and negatively related to long‐term trends in temperature. Underlying processes that could explain such a relationship are discussed, with special focus on changes in migration patterns of the Atlantic bluefin tuna. 相似文献