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101.
102.
[目的]为深入了解贵州省农村信息化服务现状,以期更科学地探索与设计贵州省农村信息化服务模式,为广大农民提供优质的信息服务.[方法]对贵州省所有地州市典型县份、典型乡(镇)和行政村的农户以进村入户、现场调查、走访、座谈和发放问卷等方式进行实地调查和数据采集,并利用SPSS 19.0统计分析软件对数据进行分析处理.[结果]贵州省农村信息化服务基础设施建设成效显著、信息传播渠道呈立体化发展、信息服务内容丰富.但也存在基础设施运行效率不高、信息传播渠道不够畅通、信息资源质量不高、信息支付意愿不强等问题.[建议]应加大基础设施建设力度、提高基础设施利用率,整合各种信息传播途径、畅通信息服务渠道,加大信息资源建设力度、提高信息内容质量,加强教育力度、提高信息意识,以推动贵州省农村信息化服务快速发展. 相似文献
103.
Improvements of TKC Technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice Plants
He Yubing Zhu Min Wang Lihao Wu Junhua Wang Qiaoyan Wang Rongchen Zhao Yunde 《水稻科学》2019,26(2):109-117
Elimination of the CRISPR/Cas9 constructs in edited plants is a prerequisite for assessing genetic stability, conducting phenotypic characterization, and applying for commercialization of the plants. However, removal of the CRISPR/Cas9 transgenes by genetic segregation and by backcross is laborious and time consuming. We previously reported the development of the transgene killer CRISPR (TKC) technology that uses a pair of suicide genes to trigger self-elimination of the transgenes without compromising gene editing efficiency. The TKC technology enables isolation of transgene-free CRISPR-edited plants within a single generation, greatly accelerating crop improvements. Here, we presented two new TKC vectors that show great efficiency in both editing the target gene and in undergoing self-elimination of the transgenes. The new vectors replaced the CaMV35S promoter used in our previous TKC vector with two rice promoters to drive one of the suicide genes, providing advantages over our previous TKC vector under certain conditions. The vectors reported here offered more options and flexibility to conduct gene editing experiments in rice. 相似文献
104.
105.
Kai WANG Hongze SHAO Zhihua PEI Guixue HU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):125-128
The aim of this experiment was to develop a loop-mediated isothermal amplification (LAMP)
assay and to research the recent epidemiology of contagious ecthyma in Jilin Province,
China, using the assay. A LAMP assay targeting a highly conserved region of the F1L gene
was developed to detect contagious ecthyma virus (CEV). Three hundred and sixty-five cases
from 64 flocks in 9 different areas of Jilin Province, China, from 2011 to 2014 were
tested using the LAMP assay. The results showed that the sensitivity of the LAMP assay was
100 copies of the standard plasmid, which is 100-fold higher than the sensitivity of PCR.
No cross-reactivity was observed with capripoxvirus, fowlpox virus, foot-and-mouth disease
virus serotype O, foot-and-mouth disease virus serotype Asia I and bluetongue virus. The
average positive rate was 19.73% (72/365), and the positive rate was highest in lambs aged
1–6 months. Our results demonstrated that CEV infection was very widespread in the flocks
of Jilin Province and that the LAMP assay allows for easy, rapid, accurate and sensitive
detection of CEV infection. 相似文献
106.
S.‐H. Wang H. Zhang Q. Zhang G.‐M. Jin S.‐J. Jiang D. Jiang Q.‐Y. He Z.‐P. Li 《Journal of Agronomy and Crop Science》2011,197(6):418-429
The effects of various copper (Cu) concentrations on the antioxidative system in the roots of Medicago sativa were explored. The results indicated that the Cu content of the roots reached a value of 854 μg g?1 DW at 10 μm Cu and a value of 4415 μg g?1 DW at 100 μm Cu, suggesting that M. sativa has better ability to tolerate and accumulate Cu than other Cu‐bioaccumulators, and is a potential plant for phytoremediation. Treatment with Cu resulted in a significant increment in the levels of H2O2, O2˙? and OH˙. The reduced form of ascorbate and glutathione reached a peak at 30 μm Cu, and was followed by a sharp depletion to a lower level than that of the control. In contrast, the levels of the oxidised forms of ascorbate and glutathione showed a progressive increment with increasing Cu concentrations, suggesting that the antioxidant system was unable to cope with Cu stress at higher Cu levels. Under the Cu concentrations tested, the activity of catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2) increased at lower Cu concentrations, and then decreased, reaching a maximum at 30 μm of Cu for APX and GR, at 10 μm for CAT, whereas the activities of guaiacol peroxidase (POD, EC 1.11.1.7) were gradually increased with increasing Cu concentrations. PAGE analysis of superoxide dismutase (SOD, EC 1.1.5.1.1) revealed that one band is a Mn‐SOD and five bands are identified as Cu, Zn‐SOD, whereas Fe‐SOD isoforms were not found in the roots of alfalfa. Cu at 10–100 μm increased the intensity of constitutive isozymes of CAT, APX and POD, whereas it decreased the intensity of isozymes of glucose‐6‐phosphate dehydrogenase (G6PDH, EC 1.1.1.49) significantly. The activities of lipoxygenases (LOX, EC 1.13.11.12) were gradually augmented with increasing Cu concentrations, demonstrating that LOXs are probably involved in production of lipid hydroperoxides and superoxide anion. There was a continuous and pronounced enhancement in the activity of esterase (EST, EC 3.1.1.1) in roots treated with 10–30 Cu μm , whereas EST activity in roots exposed to above 30 μm Cu declined, suggesting that EST plays a protective role under lower Cu concentrations stress. 相似文献
107.
本文着重分析了当前我国图书出版发行环境中出现的一些新的变化、新的特点和新的问题,并提出了在图书馆采访工作中,针对这些问题可采取相应的措施。 相似文献
108.
赤霉素对植物的生长发育具有重要的调控作用。综述了赤霉素调控块茎形态建成的研究进展。块茎形态建成是一个复杂的生理过程,受遗传、坏境等因素共同调控,其中,激素调控发挥重要作用。虽然多种激素参与调控块茎的形态建成,但它们的效果最终均依赖于赤霉素含量。一般认为赤霉素抑制或延缓块茎的形成,但对块茎生长膨大的作用有不同的看法。已明确植物体内赤霉素转导的GA-GID1-DELLA路径,但是否在块茎形态建成过程中发挥作用还不清楚。最后提出块茎形态建成可能分为块茎启动和生长膨大两个不同阶段,分属于两个独立的调控系统,同时存在不同于GA-GID1-DELLA信号通路的赤霉素途径,赤霉素在其中发挥的作用不同,机制也不同。 相似文献
109.
克深三维区目前处于勘探开发的前期,需要明确地应力场及地下储层裂缝的分布规律,指导开发工作,但该区存在地层埋深大、取心困难、成像测井资料缺乏等实际情况,应力场研究及裂缝预测难度大。利用有限元分析方法,以井点现今地应力值为约束进行了克深三维区现今地应力场的数值模拟,同时建立了应力应变与裂缝参数的定量关系模型,利用古今应力场结合法对克深三维区的裂缝参数进行了预测,模拟结果显示构造高部位和断层带是低应力值区及裂缝的有利发育区。裂缝的数值模拟结果与测井解释结果对比表明,预测结果与实钻吻合度较高,裂缝预测效果较好。 相似文献
110.
为分析进口越南屠宰用活猪传入重大动物疫病的风险,探讨进口越南屠宰用活猪的可行性,参照世界动物卫生组织以及澳大利亚、新西兰等的进口风险分析技术,采用定性评估方法,开展非洲猪瘟、口蹄疫、猪瘟、猪繁殖与呼吸综合征等4种进境一类重大动物疫病传入释放评估、接触发生评估和后果评估。评估认为,非洲猪瘟、口蹄疫、猪瘟、猪繁殖与呼吸综合征等疫病具有"高"传入风险,可随活猪引进病原体,并且能够对我国养猪业产生不利影响,因此目前不能接受其进口。针对"高"传入风险,需要制定严格的降低疫病传入风险管理措施,包括严格产地要求、加强境外预检、指定口岸入境、强化隔离检疫、建立生物隔离区。本研究对我国动物产品进口贸易政策及检疫措施的制定提供了有力参考。 相似文献