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321.
Subramaniam R Herndon CN Shanthalingam S Dassanayake RP Bavananthasivam J Potter KA Knowles DP Foreyt WJ Srikumaran S 《Veterinary microbiology》2011,153(3-4):332-338
The molecular and cellular basis for the enhanced lung pathology and mortality caused by Mannheimia haemolytica in bighorn sheep (BHS, Ovis canadenesis), in comparison to domestic sheep (DS, Ovis aries), is not clear. Polymorphonuclear leukocytes (PMNs) of BHS are four- to eight-fold more susceptible to M. haemolytica leukotoxin-induced cytolysis, which is likely to reduce the number of functional phagocytes in the lung. We hypothesized that enhanced lung pathology is due to defective clearance of M. haemolytica from the lungs of BHS. To test this hypothesis, M. haemolytica (1 × 10(7) colony forming units [cfu]) were inoculated intra-tracheally into three groups each of BHS and DS, which were euthanized and necropsied at 4, 12, and 18 h post-inoculation (hpi). Bacterial and leukocyte counts were performed on broncho-alveolar lavage fluid (BALF) collected at necropsy. BALF from BHS euthanized at 4 and 12 hpi contained a significantly higher number of M. haemolytica than that from DS. More importantly, DS did not have any bacteria in BALF at 18 hpi, while the BHS still had significant numbers. As expected, the BHS did exhibit more extensive lung lesions at 12 and 18 hpi when compared to DS. At 18 hpi, necrotic PMNs were observed in the lesional lung tissues of BHS, but not DS. Furthermore, BALF from BHS had significantly lower titers of antibodies to Lkt and surface antigens of M. haemolytica, than that of DS. These findings suggest that the enhanced pathology in BHS lungs is due to defective clearance of M. haemolytica from the lungs. 相似文献
322.
323.
Simpson CJ Mansfield CS Milne ME Hodge PJ 《Journal of Feline Medicine and Surgery》2011,13(10):787-792
A 6-year-old male neutered cat presented with blindness, lethargy, polydipsia, hyposthenuria and severe hypernatraemia. Central diabetes insipidus was demonstrated by means of a low measured anti-diuretic hormone (ADH) concentration in the face of hypernatraemia, and clinical response to supplementation with desmopressin. Magnetic resonance imaging of the brain showed a discrete mass in the region of the hypothalamus. The cat was euthanased and post-mortem histological examination demonstrated B cell lymphoma involving the brain, optic nerves, urinary bladder wall and diaphragm. To the authors' knowledge, this case report is the first to describe central diabetes insipidus caused by central nervous system lymphoma in the cat. 相似文献
324.
Pillai RS Bhattacharyya SN Artus CG Zoller T Cougot N Basyuk E Bertrand E Filipowicz W 《Science (New York, N.Y.)》2005,309(5740):1573-1576
MicroRNAs (miRNAs) are approximately 21-nucleotide-long RNA molecules regulating gene expression in multicellular eukaryotes. In metazoa, miRNAs act by imperfectly base-pairing with the 3' untranslated region of target messenger RNAs (mRNAs) and repressing protein accumulation by an unknown mechanism. We demonstrate that endogenous let-7 microribonucleoproteins (miRNPs) or the tethering of Argonaute (Ago) proteins to reporter mRNAs in human cells inhibit translation initiation. M(7)G-cap-independent translation is not subject to repression, suggesting that miRNPs interfere with recognition of the cap. Repressed mRNAs, Ago proteins, and miRNAs were all found to accumulate in processing bodies. We propose that localization of mRNAs to these structures is a consequence of translational repression. 相似文献
325.
Buckley M Walker A Ho SY Yang Y Smith C Ashton P Oates JT Cappellini E Koon H Penkman K Elsworth B Ashford D Solazzo C Andrews P Strahler J Shapiro B Ostrom P Gandhi H Miller W Raney B Zylber MI Gilbert MT Prigodich RV Ryan M Rijsdijk KF Janoo A Collins MJ 《Science (New York, N.Y.)》2008,319(5859):33; author reply 33
We used authentication tests developed for ancient DNA to evaluate claims by Asara et al. (Reports, 13 April 2007, p. 280) of collagen peptide sequences recovered from mastodon and Tyrannosaurus rex fossils. Although the mastodon samples pass these tests, absence of amino acid composition data, lack of evidence for peptide deamidation, and association of alpha1(I) collagen sequences with amphibians rather than birds suggest that T. rex does not. 相似文献
326.
Atkinson NJ Dew TP Orfila C Urwin PE 《Journal of agricultural and food chemistry》2011,59(17):9673-9682
Induction of abiotic stress in tomato plants has been proposed as a mechanism for improving the nutritional quality of fruits. However, the occurrence of biotic stress can interfere with normal abiotic stress responses. In this study, the combined effect of water stress and infection with plant-parasitic nematodes on the nutritional quality of tomato was investigated. Plants were exposed to one or both stresses, and the levels of phenolic compounds, carotenoids, and sugars in fruits were analyzed as well as physiological responses. Levels of carotenoids lycopene and β-carotene were lower in water-stressed tomatoes but exhibited a different response pattern under combined stress. Nematode stress was associated with increased flavonoid levels, albeit with reduced yields, while chlorogenic acid was increased by nematodes, water stress, and the combined stress. Sugar levels were higher only in tomatoes exposed to both stresses. These results emphasize the importance of studying plant stress factors in combination. 相似文献
327.
Eyles A Smith D Pinkard EA Smith I Corkrey R Elms S Beadle C Mohammed C 《Tree physiology》2011,31(6):592-603
The phloem-feeding aphid Essigella californica represents a potential threat to the productivity of Pinus radiata plantations in south-eastern Australia. Five- and nine-year-old field trials were used to characterize the effects of artificial and natural aphid-induced (E. californica) defoliation, respectively, on shoot photosynthesis and growth. Photosynthetic capacity (A(max)) was significantly greater following a 25% (D25) (13.8 μmol m(-2) s(-1)) and a 50% (D50) (15.9 μmol m(-2) s(-1)) single-event upper-crown artificial defoliation, 3 weeks after defoliation than in undefoliated control trees (12.9 μmol m(-2) s(-1)). This response was consistently observed for up to 11 weeks after the defoliation event; by Week 16, there was no difference in A(max) between control and defoliated trees. In the D50 treatment, this increased A(max) was not sufficient to fully compensate for the foliage loss as evidenced by the reduced diameter increment (by 15%) in defoliated trees 36 weeks after defoliation. In contrast, diameter increment of trees in the D25 treatment was unaffected by defoliation. The A(max) of trees experiencing upper-crown defoliation by natural and repeated E. californica infestations varied, depending on host genotype. Despite clear differences in defoliation levels between resistant and susceptible genotypes (17 vs. 35% of tree crown defoliated, respectively), growth of susceptible genotypes was not significantly different from that of resistant genotypes. The observed increases in A(max) in the lower crown of the canopy following attack suggested that susceptible genotypes were able to partly compensate for the loss of foliage by compensatory photosynthesis. The capacity of P. radiata to regulate photosynthesis in response to natural aphid-induced defoliation provides evidence that the impact of E. californica attack on stem growth will be less than expected, at least for up to 35% defoliation. 相似文献
328.
Graziela Sponchiado Eliana Mara Fortunato de Lucena Reynaldo Any Caroline B. de Andrade Eliane Carvalho de Vasconcelos M?nica Lucia Adam C??ntia Mara Ribas de Oliveira 《Water, air, and soil pollution》2011,218(1-4):353-360
Pharmacologically active substances used in the treatment of human and animal illnesses may usually enter the aquatic environment via effluents from sewage treatment plants, as they are not completely biodegraded or removed during waste water treatment. 17??-Estradiol genotoxicity was evaluated in Oreochromis niloticus (family Cichlidae) using micronucleus test, other nuclear abnormalities assessment, and the comet assay with erythrocytes. Fish were exposed to aqueous systems contaminated with 6 ng/L 17??-estradiol for three periods: 24 h, 48 h, and 10 days. The results showed that 17??-estradiol has genotoxic potential in different periods, since significant differences (P?=?0.036) were observed in the micronucleus frequencies of the 10-day exposure groups in relation to the control group. Also, the same was observed when comparing the nuclear abnormality frequencies (P?=?0.018) of the 24-h exposure group with the negative control group, and when using comet assay (P?<?0.001) for 48-h evaluations. The tested concentration of the 17??-estradiol gave rise to mutagenic and genotoxic effects on the blood cells of O. niloticus, therefore the substance being considered a clastogenic chemical contaminant on both acute and chronic exposures. The assessment using a combination of micronucleus test, nuclear abnormalities, and comet assays proved to be suitable and useful in the genotoxicity testing of 17??-estradiol at nanograms per liter. 相似文献
329.
A technique for searching full model space that was applied to measurements of anomalously split normal modes showed a robust pattern of P-wave and S-wave anisotropy in the inner core. The parameter describing P-wave anisotropy changes sign around a radius of 400 kilometers, whereas S-wave anisotropy is small in the upper two-thirds of the inner core and becomes negative at greater depths. Our results agree with observed travel-time anomalies of rays traveling at epicentral distances varying from 150 degrees to 180 degrees. The models may be explained by progressively tilted hexagonal close-packed iron in the upper half of the inner core and could suggest a different iron phase in the center. 相似文献
330.
Whalen PJ Kagan J Cook RG Davis FC Kim H Polis S McLaren DG Somerville LH McLean AA Maxwell JS Johnstone T 《Science (New York, N.Y.)》2004,306(5704):2061
The amygdala was more responsive to fearful (larger) eye whites than to happy (smaller) eye whites presented in a masking paradigm that mitigated subjects' awareness of their presence and aberrant nature. These data demonstrate that the amygdala is responsive to elements of. 相似文献