全文获取类型
收费全文 | 6639篇 |
免费 | 309篇 |
国内免费 | 749篇 |
专业分类
林业 | 789篇 |
农学 | 1051篇 |
基础科学 | 608篇 |
1187篇 | |
综合类 | 1873篇 |
农作物 | 297篇 |
水产渔业 | 269篇 |
畜牧兽医 | 846篇 |
园艺 | 247篇 |
植物保护 | 530篇 |
出版年
2024年 | 19篇 |
2023年 | 73篇 |
2022年 | 202篇 |
2021年 | 277篇 |
2020年 | 231篇 |
2019年 | 252篇 |
2018年 | 163篇 |
2017年 | 218篇 |
2016年 | 223篇 |
2015年 | 297篇 |
2014年 | 273篇 |
2013年 | 313篇 |
2012年 | 440篇 |
2011年 | 407篇 |
2010年 | 433篇 |
2009年 | 361篇 |
2008年 | 387篇 |
2007年 | 357篇 |
2006年 | 346篇 |
2005年 | 359篇 |
2004年 | 149篇 |
2003年 | 175篇 |
2002年 | 90篇 |
2001年 | 126篇 |
2000年 | 154篇 |
1999年 | 210篇 |
1998年 | 184篇 |
1997年 | 149篇 |
1996年 | 141篇 |
1995年 | 109篇 |
1994年 | 101篇 |
1993年 | 86篇 |
1992年 | 88篇 |
1991年 | 61篇 |
1990年 | 72篇 |
1989年 | 50篇 |
1988年 | 45篇 |
1987年 | 27篇 |
1986年 | 14篇 |
1985年 | 5篇 |
1984年 | 6篇 |
1983年 | 9篇 |
1982年 | 6篇 |
1981年 | 6篇 |
1980年 | 3篇 |
排序方式: 共有7697条查询结果,搜索用时 0 毫秒
51.
利用pH调节法制备罗非鱼分离鱼蛋白.在临界pH条件下对罗非鱼肌肉蛋白进行处理得到肌肉溶解蛋白(Solution Muscle Protein,SMP),再利用等电点沉淀法对罗非鱼肌肉溶解蛋白进行分离得到肌肉分离蛋白(Isolated Muscle Protein,IMP),考查SMP的溶解率、黏度及IMP凝胶制品和常规中性漂洗(Conventional Wash)处理得到的蛋白凝胶制品的凝胶强度.以罗非鱼肌肉蛋白质在不同pH下的处理、转谷酰胺酶、食盐及凝胶化温度为因素,凝胶强度为目标参数进行正交试验设计.结果表明,与常规中性漂洗法相比, pH调节法制备的罗非鱼分离鱼蛋白具有回收效果好、功能性强的特点. 相似文献
52.
为探究怀山药皮(Chinese yam peel,YP)对淇河鲫免疫的影响,选取平均体重为(106.76±3.74)g的淇河鲫240尾,随机分为对照组(NC)、0.5%怀山药皮添加组(LYP)、1%怀山药皮添加组(MYP)、2%怀山药皮添加组(HYP)4组,养殖8周,检测实验鱼生长指标、血清生化指标及免疫相关基因的表达情况。结果显示:添加怀山药皮对淇河鲫生长指标没有显著性影响;血液生化指标中谷草转氨酶活性在MYP组显著性降低,碱性磷酸酶的活性显著升高;与NC组相比,怀山药皮添加组肝脏细胞膜与核膜完整性增强,胞质破碎,核溶解、偏移现象减轻;与NC组相比,添加怀山药皮上调了抑炎因子(IL-10)、紧密连接蛋白基因(Claudin-1)、抗氧化基因(CAT和GST)和TLR通路基因(TLR4)mRNA表达量。以上结果表明,饲料中添加怀山药皮,能在一定程度上增强鱼体免疫力。 相似文献
53.
以江苏省吴江市八坼镇区域养殖场加州鲈鱼病鱼的细菌性病原体为研究对象。通过分离纯化,形态学观察,结合16S rRNA基因序列同源性检索,对该病原菌进行初步判断。同时进行药敏实验,了解该病原菌对各种抗生素的耐药性和敏感程度。研究结果:分离纯化得到2株纯菌株L2F和L2P2,革兰染色均呈红色,阴性杆状菌。16S rDNA序列分析,L2F和L2P2菌株与迟缓爱德华氏菌(Edwardsiella tarda)的同源性达99%。初步判断该菌为迟缓爱德华氏菌。药敏实验结果,该菌对磺胺异恶唑、链霉素和利福平等药物不敏感或耐药,对左氧氟沙星、诺氟沙星、恩诺沙星、强力霉素、氟苯尼考、头孢哌酮相对敏感。 相似文献
54.
大蒜为百合科葱属植物蒜的鳞茎。大蒜的幼苗、花茎、鳞茎均为广大群众所喜食,不仅可以生食和做成调味品,还可以做成糖醋蒜、罐头和大蒜油等产品出口创汇。大蒜栽培历史有2000多年,大蒜是人们日常生活不可缺少的辛香类蔬菜。大蒜含有丰富的糖类、蛋白质、脂肪、粗纤维,数量元素、维生素和大蒜素,营养与保健价值极高,被誉为“天然广谱杀菌素”。 相似文献
55.
1、苹果腐烂病 1.1为害症状苹果树腐烂病有溃疡型、枝枯型两种.溃疡型病斑是冬春发病盛期和夏季在极度衰弱树上发生的典型症状.病斑最初在外表,不易被发现,揭开枝干表皮后,可见暗褐色湿润状小斑或黄褐色干斑,当环境条件适宜时,在夏秋落皮层上出现稍带红褐色、稍湿润的小溃疡斑. 相似文献
56.
57.
58.
59.
Youji Wang Menghong Hu Weimin Wang Ling Cao Yi Yang Biping Lü Rongrong Yao 《Aquaculture International》2008,16(6):539-549
The diel feeding rhythm and ontogenesis during early life stage of loach Misgurnus anguillicaudatus were investigated under experimental conditions (light: L 06:00–18:00, D 18:00–06:00 h). Morphological and behavioral developments
of loach from newly hatched to 40 days after hatching were observed. Larvae were able to prey on daphnia 3–4 days after hatching
at 23 ± 0.5°C. As the larvae grew, they showed an increasing feeding capacity and a distinct feeding rhythm. Feeding intensity
and incidence for day-4 larvae were highest at 10:00 and 16:00 h. The highest levels of feeding intensity for day-12 larvae
occurred at 08:00, 12:00, and 18:00 h as did feeding incidence. By day 20, when the larvae metamorphosed, the highest levels
of feeding intensity occurred at 06:00, 18:00, and 24:00 h and were concurrent with the highest feeding incidence. After metamorphosis,
feeding capacity had again increased considerably and, in contrast to the earlier stages before day 20, feeding intensity
for day-30 juveniles peaked at 05:00 and 20:00 h, about 1–2 h after the maximum feeding incidence. The feeding rhythm of loach
juveniles at day 40 was almost the same as the day-30 juveniles. The estimated maximum daily feeding rates were 43.1%, 33.4%,
19.0%, 12.8%, and 5.8% of body weight on days 4, 12, 20, 30, and 40, respectively. Thus, loach was found to have different
feeding rhythms in the pre- and post-metamorphosis stages, with the highest feeding activity in daytime during the larval
planktonic stage before metamorphosis, and intensely nocturnal feeding behavior during the juvenile benthic stage after metamorphosis. 相似文献
60.
Jia-Rui Wu Fang-Ji Xu Wei Cao Wei Zhang Chuan-Chao Dai 《Archives of Agronomy and Soil Science》2019,65(2):240-252
Previously, the application of the fungal endophyte Phomopsis liquidambari B3 exhibited notable effect in alleviating soil obstacles caused by continuous cropping of peanut. It was supposed that P. liquidambari B3 could enhance the efficiency of nodulation and nitrogen fixation by enriching the diversity of nodular endophytic bacteria under continous monocropping system. To verify this hypothesis, a pot experiment was conducted under natural conditions. Four treatments were set as follow: discontinuous cropping soil (NS), continuous cropping soil (CK), continuous cropping soil inoculate with actived P. liquidambari B3 (CSB3), and continuous cropping soil inoculate with sterilized P. liquidambari B3 (CSIB3). As a result, a total of 120 isolates were obtained. Our study clearly declared that the addition of fungal endophyte effectively enriched the genetic diversity and the community composition in CSB3. In contrast, a low genetic diversity level and simplex community structure were exhibited in CK and CSIB3. Meanwhile, the positive percentage rate and corresponding community composition of the plant growth-promoting isolates in NS and CSB3 were larger and more abundant than the other. This is the first time to describe the effects of fungal endophytes on the diversity of the nodular culturable endophytic bacteria associated with continuous cropping of peanut. 相似文献