有效积温对中华常春藤生长的影响

通过调查中华常春藤的生境和测量茎的生长情况,总结了中华常春藤的种植特点和分枝方式,同时利用SPSS软件的回归分析建立回归模型,并根据建立的模型用Excel软件绘制坐标图。结果表明:中华常春藤主枝生长完毕所需的有效积温为2 718.34℃/d;茎伸长量随有效积温延续的变化呈"S"形曲线,而茎伸长速度变化趋于钟罩型单峰曲线。再利用此结果估算出:在理想的生长条件下采用三角形定植方式定植中华常春藤为宜,并且植株与植株之间的最佳定植密度为510 mm×510 mm。     

气调袋保鲜青菜头(茎瘤芥)的研究

检测青菜头贮藏过程中MA袋内气体指标变化和贮藏后的品质指标,并进一步探讨贮藏效果最佳MA袋的袋装量和硅窗面积对青菜头贮藏保鲜效果的影响。结果表明,PE硅窗袋、PVC硅窗袋和SCA袋内气体指标在一定时间后达到相对平衡,定期换气袋内气体呈不稳定周期性变化。PVC硅窗袋贮藏效果较好,PE硅窗袋和SCA袋次之,定期换气袋较差。当袋装量15 kg,硅窗面积为110 mm×75 mm时,PVC硅窗袋贮藏保鲜效果达到最好。     

Energy requirements of Dorper×thin-tailed Han crossbred ewes during non-pregnancy and lactation

This experiment was conducted to investigate the energy requirement of Dorper×thin-tailed Han crossbred ewes during non-pregnancy and lactation. Fifteen ewes after parturition were randomly assigned to three treatments: ad libitum(100%) feed intake and 80 or 60% ad libitum intake, and another nine non-pregnant ewes were assigned to a blank control group. Digestibility trials were performed in the non-pregnant ewes and in the lactating ewes on the 20 th, 50 th, and 80 th d of lactation. In parallel with the digestibility trial, a respirometry experiment was conducted to determine the methane and carbon dioxide production with an open-circuit respirometry system that was equipped with respiratory chambers. The net energy(NE) and metabolizable energy(ME) requirements for maintenance and growth were calculated using the carbon and nitrogen balance method. The results revealed that the carbon(C) and nitrogen(N) excretions and energy losses at faeces and urine, as well as the output of methane and CO2, increased significantly with decreasing feed intake(P0.01). The apparent digestibilities of C in th e stages of non-pregnancy and early, middle and late lactation were 55.8–58.3%, 62.5–73.8%, 64.8–71.3%, and 61.7–65.0%, respectively, and the apparent digestibilities of N were 45.2–51.3%, 73.7–82.7%, 72.8–80.5%, and 73.6–76.5%, respectively. The corresponding energy apparent digestibilities were 52.0–56.3%, 60.7–76.6%, 61.0–68.8%, and 61.4–67.7%, respectively. The ME/DE(digestible energy) values were 79.5–85.9%, 79.4–83.5%, 81.0%–85.3% and 78.6–82.9%, respectively. The maintenance requirements of NE, ME, and the efficiencies of ME utilisation for maintenance during the stages of non-pregnancy and early, middle and late lactation were 215.5, 253.1, 247.7, and 244.7 k J kg~(–1) BW0.75 d, and 372.4, 327.1, 320.9, and 362.0 k J kg~(–1) BW0.75 d, and 0.58, 0.77, 0.77, and 0.68, respectively. The ME requirement for the growth of non-pregnant ewes was 31.3 MJ kg~(–1) BW gain.     

输水渡槽最优断面优化设计

采用非线性模型,提出了输水渡槽的最优园底直角横断面。在同样水力条件下,最优园底直角断面的湿周是矩形最优断面的83.7%。     

Antiproliferation effects of 188Re labeled monoclonal antibody on prostate cancer cells in vitro

AIM: To investigate the effect of 188Re labeled monoclonal antibody on prostatic specific membrane antigen 7E11C5.3,radioimmunotherapy for the treatment of human prostate cancer cell line LNCaP in vitro.METHODS: 188Re-7E11C5.3 was prepared by direct 2-mercaptoethanol reduction method.Labeling efficiency and radiochemical purity was measured by paper chromatography.Immunoreactive fraction was determined by linear extrapolation.Cytotoxicity to LNCaP cells was determined by MTT assay.RESULTS: The labeling yield of 188Re-7E11C5.3 was (93.16±2.18)%,the radiochemical purity was (95.62±0.48)%,and the immunoreactive fraction was (74.86±1.86)%.The inhibitory effect of 188Re-7E11C5.3 on cell proliferation of LNCaP cells was significantly higher than that of 188Re-mIgG or 188ReO-4.The 50% inhibitory doses (IC50) of 188Re-7E11C5.3,188Re-mIgG,and 188ReO-4 were (23.38±3.73)×107 Bq/L,(59.21±8.02)×107 Bq/L and (68.89±10.91)×107 Bq/L,respectively.CONCLUSION: 188Re-7E11C5.3 can effectively inhibit the growth of in vitro cultured prostate cancer cells and shows much potential for prostate cancer radioimmunotherapy.     

超抗原及其在兽医科学上的应用

超抗原以极低的浓度就能引起机体免疫细胞、细胞因子和抗体的巨大变化,引起机体一系列病理生理过程。鉴于超抗原的这种特性,医学上已成功将其用于癌症和病毒病的辅助治疗。在兽医科学上,超抗原有望在兽医病理学、兽医药理学、兽医临床治疗和兽医预防方面得到广泛的应用。