Isolation and Identification of BPIV3 Genotype C

To identify the infection agents from Ningxia Hui Autonomous region, where feedlot cattle indicated bovine respiratory disease complex (BRDC), the M gene of the bovine parainfluenza virus type 3 was amplified by RT-PCR.The PCR product was ligated to pMD18-T vector and cloned to E.coli DH5α.The positive clones were sequenced and compared with the reference strains in GenBank by the molecular biology software.Sequence alignment results showed that a BPIV3 strain was isolated from the samples and named NX49, the M gene of NX49 included 1 056 nucleotides.Evolutionary analysis showed that the NX49 belonged to BPIV3 C genotype and shared 99.4% nucleotide identity with that of the SD0835 isolated in Shandong province.The characterization of the NX49 demonstrated that it was sensitive to temperature, acid and organic matter.The presence of Mg²⁺ showed no protection against the treatment at high temperature.The HA test suggested that the NX49 enables to agglutinate the guinea pig RBC at 4 ℃ and the titer was 1∶4.The study isolated a BPIV3 genotype C strain successfully, which facilitate the study of molecular evolution and epidemiology of BPIV3 in China.     

Isolation and Identification of Pathogenic Bacteria Causing Dairy Cow Mastitis in Liaoning and Drug Sensibility Test of Pathogenic E.coli

In order to understand the main bacterial pathogen species causing dairy cow mastitis in Liaoning, as well as the characteristics of drug sensitivity of the pathogenic E.coli,the milk samples from 75 dairy cows with clinical manifestations for mastitis in certain large-scale dairy farm in Liaoning were collected.The bacteria in milk were cultured and isolated with biochemical methods and in vitro drug sensitivity tests were processed with the isolated E.coli strains.The results showed that the main bacterial pathogen for dairy cow mastitis were E.coli（separation rate 58.7%),S.aureus（64.0%）and S.agalactiae（54.7%),and multiple infection including double and triple infection were identified.The drug sensitivity tests on the isolated E.coli indicated that the E.coli isolates were highly resistant to sulfonamides（resistance rate>85%）and chloramphenicol（resistance rate>30%),and they were relatively low resistant to ampicillin（9.5%),ciprofloxacin（9.5%),ceftiofur（7.1%）and ofloxacin（4.8%).The results was able to provide reliable theoretical basis for prevention and control of dairy cow mastitis in Liaoning area.     

重庆三峡库区肉牛结核病布鲁菌病的调查

采用牛提纯结核菌素皮内变态反应结合γ-干扰素试验、虎红平板凝集试验、试管凝结试验及ELISA法分别对重庆三峡库区肉牛结核病、布鲁菌病进行了抽样检测。结果显示,该区域肉牛结核病阳性检出率为1.2%,布鲁菌病阳性检出率为1.04%。其中,库区东北部区县肉牛结核病、布鲁菌病的阳性检出率均为最高,分别为1.83%、1.14%;3种肉牛养殖规模条件下,个体散养农户牛群结核病、布鲁菌病的阳性检出率均为最高,分别为2.21%、2.94%;所调查的3个年龄段牛群中,19月龄以上牛群结核病、布鲁菌病的阳性检出率最高,分别为2.91%、4.85%。     

麦草畏、水杨酸对西洋参成熟种胚体细胞胚发生的影响

以西洋参成熟种胚为试材,以不加激素和添加0.5mg/L 2,4-D的MS培养基为对照,研究了不同浓度(0.05~2.00mg/L)麦草畏、水杨酸对西洋参体胚发生的影响。结果表明:未添加激素的空白培养基没有愈伤和体胚发生;在附加2,4-D的培养基中愈伤和体胚发生率为100%;5个浓度的麦草畏和水杨酸均能诱导出体胚,其中1.00mg/L麦草畏的效果最佳,体胚发生率为56.60%,水杨酸处理下体胚发生率最高为7.50%;在各种浓度的水杨酸中种胚生长量比对照小且褐化严重。说明麦草畏、水杨酸均可诱导西洋参成熟种胚体细胞胚发生,诱导能力2,4-D麦草畏水杨酸;水杨酸对种胚生长有抑制作用。     

西瓜嫁接砧木对南方根结线虫的抗性鉴定

以16种西瓜砧木为试材,采用聚类分析和隶属函数分析方法,研究了南方根结线虫(Meloidogyne incognita)对不同砧木的生长指标和抗病指标的影响。结果表明:抗性最强的是"青农2号",隶属函数总值为6.26,抗性最弱的是"青农3号",隶属函数总值为1.48。结论认为"青农2号"为西瓜嫁接的最佳砧木,该试验为合理筛选和利用西瓜砧木提供了理论和技术参考。     

去花对菊芋数量学性状的影响

以白皮菊芋为试材,通过对现蕾期菊芋去除1/4花、1/3花、1/2花、全部去除和不去花等5个处理,测定叶片、小花、分枝和块茎数量等相关指标,探讨了去花对菊芋数量学性状的影响规律,为提高菊芋块茎生物产量提供理论依据。结果表明:去花1/3、1/2和全株去花提高了单株菊芋块茎数量,降低单株块茎质量,1/4去花对菊芋单株块茎数量和单个块茎质量影响不显著;去花处理降低了二级分枝和一级分枝的数量;去花处理增加了单株叶片的数量和单片叶片的质量;随着去花数量的增加,单株小花数量呈现"降-升-降"的变化规律,单个小花质量呈现"降-升"的变化趋势。     

野生蝉花液体菌种培养条件的优化

以野生蝉花菌种为试材,采用液体培养法,研究了碳源、氮源、微量营养和pH对野生蝉花菌种生长的影响,以期为标准化、规模化、产业化人工栽培蝉花提供优质种源。结果表明:将野生蝉花菌株接种到液体培养基中,在19℃,转速120r·min-1恒温振荡摇床内培养,蝉花菌种的最适液体培养基组分为可溶性淀粉20g,奶粉10g,磷酸二氢钾0.5g,硫酸镁1g,维生素B1210片,水1 000mL,最适pH 5.0~6.0。     

我国果树玻璃化试管苗特征、成因、机理及控制研究进展

该研究统计了我国已报道的出现玻璃化试管苗的果树种类多达50种,并基于近年果树工作者的相关研究,总结并系统论述了玻璃化试管苗的生理生化特性、形态解剖学特征、DNA的MSAP分析、成因、发生机理及控制措施,对系统研究果树试管苗玻璃化现象、增加转基因植株的遗传稳定性、保存和恢复珍贵材料无性系、提高次生代谢物产量和消除毒性及工厂化育苗都具有十分重要的意义。     

地熊蜂为重庆地区设施番茄授粉的效果研究

为评价地熊蜂在重庆地区设施番茄的授粉效果,在重庆市荣昌区龙舌嘴村开展授粉效果比较试验,分析地熊蜂授粉对设施番茄产量、果实品质及畸形果率的影响。试验结果显示,经地熊蜂授粉的设施番茄平均产量为2 206.44 kg/667 m2,与未授粉组相比显著提高了104.95%,授粉组的单果质量、果纵径、果最大横径、可溶性固形物含量、VC含量及总糖含量显著高于未授粉组。由此,认为地熊蜂适宜为重庆地区设施番茄授粉,且能够显著提高重庆地区设施番茄的产量与品质。