全文获取类型
收费全文 | 12187篇 |
免费 | 907篇 |
国内免费 | 3篇 |
专业分类
林业 | 1234篇 |
农学 | 449篇 |
基础科学 | 53篇 |
2764篇 | |
综合类 | 811篇 |
农作物 | 515篇 |
水产渔业 | 812篇 |
畜牧兽医 | 5037篇 |
园艺 | 289篇 |
植物保护 | 1133篇 |
出版年
2023年 | 104篇 |
2022年 | 134篇 |
2021年 | 245篇 |
2020年 | 240篇 |
2019年 | 166篇 |
2018年 | 571篇 |
2017年 | 533篇 |
2016年 | 488篇 |
2015年 | 403篇 |
2014年 | 465篇 |
2013年 | 693篇 |
2012年 | 948篇 |
2011年 | 893篇 |
2010年 | 480篇 |
2009年 | 373篇 |
2008年 | 753篇 |
2007年 | 717篇 |
2006年 | 582篇 |
2005年 | 606篇 |
2004年 | 559篇 |
2003年 | 511篇 |
2002年 | 431篇 |
2001年 | 289篇 |
2000年 | 253篇 |
1999年 | 226篇 |
1998年 | 76篇 |
1997年 | 65篇 |
1996年 | 46篇 |
1995年 | 63篇 |
1994年 | 44篇 |
1993年 | 40篇 |
1992年 | 89篇 |
1991年 | 73篇 |
1990年 | 67篇 |
1989年 | 72篇 |
1988年 | 56篇 |
1987年 | 52篇 |
1986年 | 54篇 |
1985年 | 49篇 |
1984年 | 48篇 |
1983年 | 37篇 |
1979年 | 40篇 |
1978年 | 22篇 |
1974年 | 27篇 |
1973年 | 24篇 |
1972年 | 25篇 |
1971年 | 30篇 |
1970年 | 35篇 |
1969年 | 28篇 |
1968年 | 30篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
71.
Clinical and in vitro efficacy of amoxicillin against bacteria associated with feline skin wounds and abscesses 总被引:1,自引:0,他引:1
Roy J Messier S Labrecque O Cox WR 《The Canadian veterinary journal. La revue veterinaire canadienne》2007,48(6):607-611
A clinical trial involving 122 cats with infected skin wounds or abscesses presented to 10 veterinary clinics was conducted to evaluate the efficacy of 2 oral amoxicillin drug products (a paste and a suspension). A 2nd objective of the study was to identify bacteria involved in such infections and verify their in vitro sensitivity to amoxicillin. Samples of wound exudate were harvested at the time of presentation and submitted for aerobic and anaerobic culture. The sensitivity to amoxicillin of isolates thought to be infecting agents was tested, using a standard minimum inhibitory concentration method. Pasteuralla multocida and obligate anaerobes of the genera Prevotella, Fusobacterium, and Porphyromonas were the most frequently isolated pathogens. Overall, their in vitro susceptibility to amoxicillin was very good. Both drug products were clinically efficacious with a global success rate of 95.1% for cats administered oral amoxicillin at 11-22 mg/kg bodyweight (mean 13.8 mg/kg bodyweight) twice daily for 7 to 10 days. 相似文献
72.
73.
Olsén L Ingvast-Larsson C Bondesson U Broström H Tjälve H Larsson P 《Journal of veterinary pharmacology and therapeutics》2007,30(3):194-200
The pharmacokinetics of the histamine H(1)-antagonist cetirizine and the effects of pretreatment with the antiparasitic macrocyclic lactone ivermectin on the pharmacokinetics of cetirizine were studied in horses. After oral administration of cetirizine at 0.2 mg/kg bw, the mean terminal half-life was 3.4 h (range 2.9-3.7 h) and the maximal plasma concentration 132 ng/mL (101-196 ng/mL). The time to reach maximal plasma concentration was 0.7 h (0.5-0.8 h). Ivermectin (0.2 mg/kg bw) given orally 1.5 h before cetirizine did not affect its pharmacokinetics. However, ivermectin pretreatment 12 h before cetirizine increased the area under the plasma concentration-time curve by 60%. The maximal plasma concentration, terminal half-life and mean residence time also increased significantly following the 12 h pretreatment. Ivermectin is an inhibitor of P-glycoprotein, which is a major drug efflux transporter in cellular membranes at various sites. The elevated plasma levels of cetirizine following the pretreatment with ivermectin may mainly be due to decreased renal secretion, related to inhibition of the P-glycoprotein in the proximal tubular cells of the kidney. The pharmacokinetic properties of cetirizine have characteristics which are suitable for an antihistamine, and this substance may be a useful drug in horses. 相似文献
74.
Recombinant bovine interferon-gamma enhances expression of class I and class II bovine lymphocyte antigens 总被引:2,自引:0,他引:2
F Walrand F Picard K McCullough S Martinod D Lévy 《Veterinary immunology and immunopathology》1989,22(4):379-383
Recombinant bovine interferon-gamma augments expression of class I and class II histocompatibility antigens on the surface membrane of bovine lymphocytes. Immunofluorescence techniques using a series of monoclonal anti-HLA antibodies demonstrate that this enhancement is detectable as early as 24 h after incubation with rBoIFN, while maximum surface expression is obtained within 3-5 days. A concentration as low as 10 units of rBoIFN is effective. Such results may be useful for characterizing the BoLA gene products. 相似文献
75.
Ruiz-Fons F Fernández-de-Mera IG Acevedo P Höfle U Vicente J de la Fuente J Gortazár C 《Veterinary parasitology》2006,140(1-2):133-142
Commercial hunting of Spanish wild ungulates has made them an important economic resource. Wild ungulates may have an important role in the maintenance of ixodid tick populations, and also as reservoirs of pathogens. We studied the ixodid ticks that parasitize Iberian red deer and European wild boar from Spain. Ixodid ticks (n=6,336) were collected from 431 Iberian red deer and 142 wild boar in different regions of Spain. We found 10 different ixodid tick species parasitizing Iberian red deer, mainly Hyalomma marginatum marginatum (63.7%), Rhipicephalus (Boophilus) annulatus (7.9%) and R. bursa (7.5%). R. (Boophilus) annulatus was only collected in the province of Cádiz (southern Spain). We found 8 ixodid tick species on the wild boar, mainly Hy. m. marginatum (68.7%), R. bursa (14.6%) and Dermacentor marginatus (9.3%). We found one adult Hy. marginatum rufipes and one adult Hy. anatolicum excavatum parasitizing wild boar from south-central Spain. Mean prevalence of ixodid ticks was 41.3+/-0.08% (n=475) and 31+/-0.09% (n=284) and intensity of parasitization was 13.9+/-0.2 (n=283) and 13.6+/-0.3 (n=130) ticks/animal for Iberian red deer and wild boar, respectively. Only 5 of the 13 ixodid tick species found were shared by Iberian red deer and wild boar. This finding could indicate a host preference when Iberian red deer and wild boar share common habitats. In both Iberian red deer and wild boar from south-central Spain the monthly relative frequencies of Hy. m. marginatum and R. bursa presented an inverse pattern. The highest Hy. m. marginatum relative frequencies coincided with the lowest R. bursa relative frequencies along the year. R. bursa and I. ricinus were present in areas from northern to southern Spain while Hyalomma sp. and D. marginatus were exclusively collected in the two southern thirds of Spain. Haemaphysalis sp. and D. reticulatus were collected in northern Spain. Hy. m. marginatum and R. bursa were present during the whole year in red deer and wild boar from south-central Spain, showing more than one life cycle per year. These results are important for understanding the role of wild ungulates in the maintenance of tick infestations and to improve tick control programmes. 相似文献
76.
Cryptosporidia, widely distributed protozoan parasites of vertebrates have recently attracted increasing interest due to several serious waterborne outbreaks, the life-threatening nature of infection in immunocompromised patients, and the realization of economic losses caused by these pathogens in livestock. Genetic polymorphism within Cryptosporidium spp. is being detected at a continuously growing rate, owing to the widespread use of modern molecular techniques. The aim of this paper is to review the current status of taxonomy, genotyping, molecular phylogeny, and characterization of cryptosporidia, and to highlight the need for polyphasic typing, i.e. an integrated approach comprising standardized morphologic, biologic, and molecular methods for describing Cryptosporidium species and isolates, and for establishing "virtual" reference strains. 相似文献
77.
Herholz CP Gerber V Tschudi P Straub R Imhof A Busato A 《American journal of veterinary research》2003,64(3):338-345
OBJECTIVE: To investigate whether volumetric capnography indices could be used to differentiate between horses without recurrent airway obstruction (RAO) and horses with RAO that were in clinical remission or that had clinically apparent RAO. ANIMALS: 70 adult Swiss Warmblood horses (20 used for pleasure riding and 50 used for dressage or show jumping). PROCEDURE: Horses were allocated to 4 groups on the basis of history, clinical signs, results of endoscopy, and cytologic findings (group 1, 21 healthy horses; group 2, 22 horses with RAO that were in remission; group 3, 16 horses with mild RAO; group 4, 11 horses with exacerbated RAO). Expiratory volume and CO2 curves were recorded by use of a computerized ultrasonic spirometer. Volumetric capnograms were plotted, and derived indices were calculated. RESULTS: Dead-space volume (VD) was calculated by use of the Bohr equation (VD(Bohr)) and for physiologic VD (VD(phys)). Ratios for VD(Bohr) to expiratory tidal volume (VT) and VD(phys) to V(T) as well as an index of effective CO2 elimination were significantly different among groups of horses. Age and use of the horses also significantly affected volumetric capnography indices. CONCLUSIONS AND CLINICAL RELEVANCE: Ratios of VD(Bohr) to VT and VD(phys) to VT as well as an index of effective CO2 elimination were sufficiently sensitive measures to distinguish between healthy horses and horses with RAO in remission. To optimize the ability of volumetric capnography indices to differentiate among horses in heterogeneous populations, it is important to account for effects of age and specific use of the horses. 相似文献
78.
Horses may be exposed to aflatoxin B(1) (AFB(1)) via inhalation of mouldy dust, leading to high exposure of olfactory and respiratory tissues. In the present study the metabolic activation of AFB(1) was examined in olfactory and respiratory tissues in horse. The results showed covalent binding of AFB(1)-metabolites in sustentacular cells and cells of Bowman's glands in the olfactory mucosa, in some cells of the surface epithelium of nasal respiratory, tracheal, bronchial and bronchiolar mucosa and in some glands in these areas. Immunohistochemistry revealed that cells expressing proteins reacting with CYP 3A4- and CYP 2A6/2B6-antibodies had a similar distribution as those having capacity to activate AFB(1). Our data indicate that the cell-specific activation of AFB(1) correlates with presence of some CYP-enzymes in olfactory and respiratory tissues in horse. 相似文献
79.
Our objective in this cross-sectional study was to determine the seroprevalence in 1996 of commercial dairy herds in central Chile positive to bovine viral diarrhea (BVD) virus, by the detection of antibodies in bulk-tank milk samples. Samples were obtained from 501 dairy farms ( approximately 50,000 cows) from the Metropolitan, VI, VII and VIII regions of Chile. An indirect ELISA was used to detect BVD antibodies. An absorbance (A450) > or = 0.55 was considered positive. The apparent proportion of positive herds ranged from 71.2 to 83% by region and did not differ by region. We concluded that exposure to BVD was widely distributed in the central area of Chile in 1996. 相似文献
80.
Kóbori L Németh T Nemes B Dallos G Sótonyi P Fehérvári I Patonai A Slooff MJ Járay J De Jong KP 《Acta veterinaria Hungarica》2003,51(4):529-537
Hepatic artery thrombosis is a major cause of graft failure in liver transplantation. Use of donor interponates are common, but results are controversial because of necrosis or thrombosis after rejection. Reperfusion injury, hypoxia and free radical production determinate the survival. The aim of the study was to create an 'ideal' arterial interponate. Autologous, tubular graft lined with mesothelial cells, prepared from the posterior rectus fascia sheath, was used for iliac artery replacement in eight mongrel dogs for six months under immunosuppression. Patency rate was followed by Doppler ultrasound. Eight grafts remained patent and another two are patent after one year. The patency rate was good (median Doppler flow: 370 cm/sec) and there was no necrosis, thrombosis or aneurysmatic formation. The grafts showed viable morphology with neoangiogenesis, appearance of elastin, smooth muscle and endothelial cells. Electron microscopy showed intact mitochondrial structures without signs of hypoxia. Tissue oxygenation was good in all cases with normal (< 30 ng/ml) myeloperoxidase production. In conclusion, this autologous graft presents good long-term patency rate. Viability, arterialisation and low thrombogenicity are prognostic factors indicating usability of the graft in the clinical practice without the risk of rejection. Further investigations such as cell cultures and standardisation are necessary. 相似文献